Samia BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= S06A01NCLL0008_E24
         (465 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC4F6.12 |||LIM domain|Schizosaccharomyces pombe|chr 2|||Manual      52   3e-08
SPAC29A4.11 |rga3||GTPase activating protein Rga3|Schizosaccharo...    40   1e-04
SPBC3F6.05 |rga1||GTPase activating protein Rga1|Schizosaccharom...    39   4e-04
SPBC28E12.03 |rga4||GTPase activating protein Rga4|Schizosacchar...    34   0.012
SPAC32A11.02c |||conserved fungal protein|Schizosaccharomyces po...    27   1.1  
SPCC4B3.18 |||phosphopantothenate-cysteine ligase|Schizosaccharo...    27   1.4  
SPAC144.05 |||ATP-dependent DNA helicase|Schizosaccharomyces pom...    27   1.9  
SPCC74.06 |mak3|phk2|histidine kinase Mak3 |Schizosaccharomyces ...    26   2.5  
SPAC18G6.09c |||sequence orphan|Schizosaccharomyces pombe|chr 1|...    25   5.7  
SPBC29A10.10c |||tRNA-splicing endonuclease positive effector |S...    25   7.5  
SPBC6B1.05c |||ubiquitin-like conjugating enzyme|Schizosaccharom...    24   9.9  

>SPBC4F6.12 |||LIM domain|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 438

 Score = 52.4 bits (120), Expect = 3e-08
 Identities = 33/113 (29%), Positives = 48/113 (42%), Gaps = 5/113 (4%)
 Frame = +2

Query: 137 KFCCALRDRCQLGDPR----KTIAGTKKMEYKTRQWHEKCFCCVVCKNPIGTK-SFIPRE 301
           KF C L D  +   PR    KT    + +      +HE    C  C          I R+
Sbjct: 303 KFYCHL-DYHEQFSPRCKHCKTPIEDQAVHINNDWFHENHHFCAGCSEVFNVNIPCIYRD 361

Query: 302 QEIYCAGCYEDKFATRCVKCNKIITQGGVTYKNEPWHRECFTCPHCNTSLAGQ 460
              +C  CY++K+A +C KC K I    V   +  +H +C+TC  CN  L  +
Sbjct: 362 DLYWCQTCYDNKYAVKCKKCRKPILGISVKGSDGEYHSQCWTCGACNALLGDE 414



 Score = 46.4 bits (105), Expect = 2e-06
 Identities = 21/73 (28%), Positives = 32/73 (43%)
 Frame = +2

Query: 224 RQWHEKCFCCVVCKNPIGTKSFIPREQEIYCAGCYEDKFATRCVKCNKIITQGGVTYKNE 403
           ++ H +CF C  C   +    F  RE + YC   Y ++F+ RC  C   I    V   N+
Sbjct: 276 KKLHPQCFKCDTCSQNLEHVGFYYREGKFYCHLDYHEQFSPRCKHCKTPIEDQAVHINND 335

Query: 404 PWHRECFTCPHCN 442
            +H     C  C+
Sbjct: 336 WFHENHHFCAGCS 348


>SPAC29A4.11 |rga3||GTPase activating protein
           Rga3|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 969

 Score = 40.3 bits (90), Expect = 1e-04
 Identities = 20/77 (25%), Positives = 29/77 (37%), Gaps = 3/77 (3%)
 Frame = +2

Query: 230 WHEKCFCCVVCKNPIGTKSFI---PREQEIYCAGCYEDKFATRCVKCNKIITQGGVTYKN 400
           WH+ CFCC  C   +     +     +    C+ C     A  C  C   I    +    
Sbjct: 40  WHKDCFCCTKCDKGLEHSDQMLVQTSDGRPVCSSC-----AHTCTACRMRIKDYALMSGY 94

Query: 401 EPWHRECFTCPHCNTSL 451
           + +HRECF C  C   +
Sbjct: 95  DSYHRECFRCHDCRKQI 111



 Score = 31.5 bits (68), Expect = 0.065
 Identities = 11/41 (26%), Positives = 21/41 (51%), Gaps = 2/41 (4%)
 Frame = +2

Query: 335 KFATRCVKCNKIIT--QGGVTYKNEPWHRECFTCPHCNTSL 451
           K +T C +C +     +  +++    WH++CF C  C+  L
Sbjct: 14  KGSTVCFRCGQAFQRRETPISFGGHMWHKDCFCCTKCDKGL 54



 Score = 28.3 bits (60), Expect = 0.61
 Identities = 11/40 (27%), Positives = 20/40 (50%)
 Frame = +2

Query: 230 WHEKCFCCVVCKNPIGTKSFIPREQEIYCAGCYEDKFATR 349
           +H +CF C  C+  I   +F    + I+C  C + +  +R
Sbjct: 97  YHRECFRCHDCRKQIIDSNFKRDNRTIFCNDCKQVRHPSR 136


>SPBC3F6.05 |rga1||GTPase activating protein
           Rga1|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 1150

 Score = 38.7 bits (86), Expect = 4e-04
 Identities = 22/71 (30%), Positives = 34/71 (47%), Gaps = 5/71 (7%)
 Frame = +2

Query: 224 RQWHEKCFCCVVCKNPIGTK-SFIPREQEIYCAGCYEDKFATRCVKCN-KIITQGGVTYK 397
           +++H + F C +C    G   S+   E ++YC   Y   FA RC  C+  I+ Q    Y+
Sbjct: 197 KKFHIEHFTCSLCYTVFGPNDSYYEYEGKVYCHYHYSTLFAARCCGCDGPILRQFVEVYR 256

Query: 398 N---EPWHREC 421
           N   + WH  C
Sbjct: 257 NGVSQNWHVPC 267



 Score = 35.1 bits (77), Expect = 0.005
 Identities = 21/77 (27%), Positives = 32/77 (41%), Gaps = 5/77 (6%)
 Frame = +2

Query: 230 WHEKCFCCVVCKNPIGTKSF-----IPREQEIYCAGCYEDKFATRCVKCNKIITQGGVTY 394
           +H +CF C  C + + +K F        +Q   C   Y  +    C  C   +    +T 
Sbjct: 135 YHLECFRCHDCNSLVASKFFPIDDPTLNKQVPLCETDYFRRLDLLCASCGMALRGYYITA 194

Query: 395 KNEPWHRECFTCPHCNT 445
            N+ +H E FTC  C T
Sbjct: 195 LNKKFHIEHFTCSLCYT 211



 Score = 31.9 bits (69), Expect = 0.050
 Identities = 12/37 (32%), Positives = 19/37 (51%)
 Frame = +2

Query: 350 CVKCNKIITQGGVTYKNEPWHRECFTCPHCNTSLAGQ 460
           C  C ++I+   V      +H ECF C  CN+ +A +
Sbjct: 116 CASCGQVISGQYVRALGNIYHLECFRCHDCNSLVASK 152


>SPBC28E12.03 |rga4||GTPase activating protein
           Rga4|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 933

 Score = 33.9 bits (74), Expect = 0.012
 Identities = 12/35 (34%), Positives = 19/35 (54%), Gaps = 1/35 (2%)
 Frame = +2

Query: 350 CVKC-NKIITQGGVTYKNEPWHRECFTCPHCNTSL 451
           C+KC   + +   V +  + WH +CF C +CN  L
Sbjct: 24  CIKCWESVPSTSQVWFGGKCWHSDCFKCVNCNKKL 58



 Score = 31.9 bits (69), Expect = 0.050
 Identities = 19/85 (22%), Positives = 37/85 (43%), Gaps = 3/85 (3%)
 Frame = +2

Query: 185 KTIAGTKKMEYKTRQWHEKCFCCVVCKNPI--GTKSFIPREQ-EIYCAGCYEDKFATRCV 355
           +++  T ++ +  + WH  CF CV C   +   ++ F   +Q +I+C  C +      C 
Sbjct: 29  ESVPSTSQVWFGGKCWHSDCFKCVNCNKKLDPSSEDFSQDDQKQIFCKLCVD-----ICN 83

Query: 356 KCNKIITQGGVTYKNEPWHRECFTC 430
            C+  I +      ++  H  C  C
Sbjct: 84  GCSTPICEFNAVSNSQANHPCCSNC 108


>SPAC32A11.02c |||conserved fungal protein|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 851

 Score = 27.5 bits (58), Expect = 1.1
 Identities = 15/48 (31%), Positives = 24/48 (50%)
 Frame = +2

Query: 11  SRLHLTMKTVGQDRIRRARENNEDVAILSMFLPNSTADRENKKFCCAL 154
           S LH + K  G+D   R   + E+ A+ ++ LP+    R  +K   AL
Sbjct: 651 SNLHKSSKEKGKDSQVRVALHKENTALNNIVLPSGNLQRSEEKRRAAL 698


>SPCC4B3.18 |||phosphopantothenate-cysteine
           ligase|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 316

 Score = 27.1 bits (57), Expect = 1.4
 Identities = 14/37 (37%), Positives = 23/37 (62%)
 Frame = -3

Query: 406 RLVLVRHTALSDYLVTLNAASGKLVFIAPGAVDLLFA 296
           RL+++ +T L++YL  L A + +L  IA  A+  L A
Sbjct: 143 RLIMIPYTTLTEYLWYLKAIAERLQIIASRALFYLAA 179


>SPAC144.05 |||ATP-dependent DNA helicase|Schizosaccharomyces
            pombe|chr 1|||Manual
          Length = 1375

 Score = 26.6 bits (56), Expect = 1.9
 Identities = 14/42 (33%), Positives = 19/42 (45%), Gaps = 8/42 (19%)
 Frame = +2

Query: 350  CVKCNKIITQGGVT--------YKNEPWHRECFTCPHCNTSL 451
            C+ C  II QG +T        +  E W +   +CP C T L
Sbjct: 1092 CIICRDIIKQGFITTCGHLYCSFCLEAWLKHSSSCPMCKTKL 1133


>SPCC74.06 |mak3|phk2|histidine kinase Mak3 |Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 2344

 Score = 26.2 bits (55), Expect = 2.5
 Identities = 13/27 (48%), Positives = 16/27 (59%), Gaps = 4/27 (14%)
 Frame = -2

Query: 191 SFSSGHRADIYPLK----HNRTSCSPY 123
           S+S G+R DIY L     H  + CSPY
Sbjct: 209 SYSVGYRTDIYSLGVLFFHYLSDCSPY 235


>SPAC18G6.09c |||sequence orphan|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 312

 Score = 25.0 bits (52), Expect = 5.7
 Identities = 13/33 (39%), Positives = 20/33 (60%)
 Frame = -3

Query: 361 TLNAASGKLVFIAPGAVDLLFARDKALRTDRVL 263
           TLN+ +  +   +  A+DLLF RD+  R  R+L
Sbjct: 275 TLNSPNNGIHCHSTSALDLLFHRDREQRLFRML 307


>SPBC29A10.10c |||tRNA-splicing endonuclease positive effector
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 1944

 Score = 24.6 bits (51), Expect = 7.5
 Identities = 9/17 (52%), Positives = 12/17 (70%)
 Frame = -3

Query: 328 IAPGAVDLLFARDKALR 278
           + PG + LLF+RD  LR
Sbjct: 188 LVPGVISLLFSRDDELR 204


>SPBC6B1.05c |||ubiquitin-like conjugating
           enzyme|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 649

 Score = 24.2 bits (50), Expect = 9.9
 Identities = 10/16 (62%), Positives = 12/16 (75%)
 Frame = +1

Query: 241 MFLLRRMQEPDRYEEL 288
           MF+LR + EPD  EEL
Sbjct: 606 MFVLRAINEPDYVEEL 621


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,796,051
Number of Sequences: 5004
Number of extensions: 34779
Number of successful extensions: 101
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 91
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 100
length of database: 2,362,478
effective HSP length: 67
effective length of database: 2,027,210
effective search space used: 176367270
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)