Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= S06A01NCLL0008_E15
(514 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC354.12 |gpd3||glyceraldehyde 3-phosphate dehydrogenase Gpd3|... 217 1e-57
SPBC32F12.11 |tdh1|gpd1|glyceraldehyde-3-phosphate dehydrogenase... 213 2e-56
SPBC1718.06 |msp1|mgm1|mitochondrial GTPase Msp1|Schizosaccharom... 29 0.41
SPCC1827.03c |||acetyl-CoA ligase |Schizosaccharomyces pombe|chr... 27 1.6
SPCC825.01 |||ribosome biogenesis ATPase, Arb family |Schizosacc... 27 2.2
SPAC22F3.03c |rdh54|tid1, mug34|ATP-dependent DNA helicase Rdh54... 26 2.9
SPBC29A10.15 |orc1|orp1, cdc30|origin recognition complex subuni... 25 5.0
SPBC660.13c |ssb1|rpa1, rad11|DNA replication factor A subunit S... 25 6.7
SPAC19A8.08 |upf2||nonsense-mediated decay protein Upf2|Schizosa... 25 8.8
SPCC584.03c |||RanGTP-binding protein |Schizosaccharomyces pombe... 25 8.8
SPBP4H10.10 |||rhomboid family protease|Schizosaccharomyces pomb... 25 8.8
>SPBC354.12 |gpd3||glyceraldehyde 3-phosphate dehydrogenase
Gpd3|Schizosaccharomyces pombe|chr 2|||Manual
Length = 335
Score = 217 bits (529), Expect = 1e-57
Identities = 104/148 (70%), Positives = 121/148 (81%), Gaps = 1/148 (0%)
Frame = +3
Query: 72 KIGINGFGRIGRLVLRASIDKGA-DVVAINDPFIGLDYMVYLFQYDSTHGRFKGTVEAVD 248
K+GINGFGRIGR+VLR +I G VVA+NDPFI LDYM Y+F+YDSTHGRF+G+VE
Sbjct: 5 KVGINGFGRIGRIVLRNAILTGKIQVVAVNDPFIDLDYMAYMFKYDSTHGRFEGSVETKG 64
Query: 249 GHLVVNGKKIAVFSERDPHAIPWGQAGAEYVVESTGVFTTTDKASAHLVGGAKKVIISAP 428
G LV++G I V +ERDP I W +GAEYV+ESTGVFTT + ASAHL GGAK+VIISAP
Sbjct: 65 GKLVIDGHSIDVHNERDPANIKWSASGAEYVIESTGVFTTKETASAHLKGGAKRVIISAP 124
Query: 429 SADAPMFVVGVNLEAYDPSYKVISNASC 512
S DAPMFVVGVNLE ++PS KVISNASC
Sbjct: 125 SKDAPMFVVGVNLEKFNPSEKVISNASC 152
>SPBC32F12.11 |tdh1|gpd1|glyceraldehyde-3-phosphate dehydrogenase
Tdh1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 336
Score = 213 bits (519), Expect = 2e-56
Identities = 102/148 (68%), Positives = 120/148 (81%), Gaps = 1/148 (0%)
Frame = +3
Query: 72 KIGINGFGRIGRLVLR-ASIDKGADVVAINDPFIGLDYMVYLFQYDSTHGRFKGTVEAVD 248
K+GINGFGRIGR+VLR A + K VVAINDPFI L+YM Y+F+YDSTHGRF G+VE D
Sbjct: 5 KVGINGFGRIGRIVLRNALVAKTIQVVAINDPFIDLEYMAYMFKYDSTHGRFDGSVEIKD 64
Query: 249 GHLVVNGKKIAVFSERDPHAIPWGQAGAEYVVESTGVFTTTDKASAHLVGGAKKVIISAP 428
G LV++G I V +ERDP I W +GA+YV+ESTGVFTT + ASAHL GGAK+VIISAP
Sbjct: 65 GKLVIDGNAIDVHNERDPADIKWSTSGADYVIESTGVFTTQETASAHLKGGAKRVIISAP 124
Query: 429 SADAPMFVVGVNLEAYDPSYKVISNASC 512
S DAPM+VVGVN E ++PS KVISNASC
Sbjct: 125 SKDAPMYVVGVNEEKFNPSEKVISNASC 152
>SPBC1718.06 |msp1|mgm1|mitochondrial GTPase
Msp1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 903
Score = 29.1 bits (62), Expect = 0.41
Identities = 22/72 (30%), Positives = 32/72 (44%), Gaps = 4/72 (5%)
Frame = +3
Query: 45 KYTLTIIMSKIGINGFGRIGRLVLRASIDKGADVVAINDPF----IGLDYMVYLFQYDST 212
KY L +S + G GR+ L++ SI K D + PF + Y++ Q D
Sbjct: 627 KYKLDSCVSTLTRMGLGRVSTLLVTDSISKCIDEITKASPFADHPAAMQYIMNAAQ-DIL 685
Query: 213 HGRFKGTVEAVD 248
RF T E V+
Sbjct: 686 RRRFHATSEQVE 697
>SPCC1827.03c |||acetyl-CoA ligase |Schizosaccharomyces pombe|chr
3|||Manual
Length = 512
Score = 27.1 bits (57), Expect = 1.6
Identities = 10/20 (50%), Positives = 15/20 (75%)
Frame = +1
Query: 79 ELTVLVASVAWCSVLRLIRV 138
+L+V VA +AWC RL+R+
Sbjct: 122 KLSVAVAELAWCPKSRLVRI 141
>SPCC825.01 |||ribosome biogenesis ATPase, Arb family
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 822
Score = 26.6 bits (56), Expect = 2.2
Identities = 12/28 (42%), Positives = 19/28 (67%)
Frame = -3
Query: 272 LSVYDEVSIYGLNGTLETTVSRIILEKV 189
L + V++ G NG +TT+ ++ILEKV
Sbjct: 617 LDLKSRVALVGPNGAGKTTLIKLILEKV 644
>SPAC22F3.03c |rdh54|tid1, mug34|ATP-dependent DNA helicase
Rdh54|Schizosaccharomyces pombe|chr 1|||Manual
Length = 811
Score = 26.2 bits (55), Expect = 2.9
Identities = 11/42 (26%), Positives = 22/42 (52%)
Frame = -3
Query: 134 LINRSTEHQATDATKTVNSDFRHDYGKSIFSQRSHSTFTLHK 9
LI + +HQ+T+ TKT + H+ ++ + S ++K
Sbjct: 11 LIESTIKHQSTNYTKTETATTSHEVSENANEHKGKSNIDINK 52
>SPBC29A10.15 |orc1|orp1, cdc30|origin recognition complex subunit
Orc1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 707
Score = 25.4 bits (53), Expect = 5.0
Identities = 12/27 (44%), Positives = 18/27 (66%), Gaps = 2/27 (7%)
Frame = -2
Query: 303 GDPSQRTQL--SSFRLRRGVHLRPQRY 229
G P++R L +F +RRGVHL+ +Y
Sbjct: 163 GIPTKRKDLFPCNFFIRRGVHLKVNKY 189
>SPBC660.13c |ssb1|rpa1, rad11|DNA replication factor A subunit Ssb1
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 609
Score = 25.0 bits (52), Expect = 6.7
Identities = 19/72 (26%), Positives = 36/72 (50%), Gaps = 10/72 (13%)
Frame = +3
Query: 45 KYTLTIIMS----KIGINGFGRIGRLVLRASIDKGADVVAINDPFIGLD------YMVYL 194
+Y +TI + ++ +N F +G+L++ + D+ D+ ND ++ YM Y+
Sbjct: 508 RYIITIAVGDHTGQLWLNVFDDVGKLIMHKTADELNDLQE-NDENAFMNCMAEACYMPYI 566
Query: 195 FQYDSTHGRFKG 230
FQ + FKG
Sbjct: 567 FQCRAKQDNFKG 578
>SPAC19A8.08 |upf2||nonsense-mediated decay protein
Upf2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1049
Score = 24.6 bits (51), Expect = 8.8
Identities = 19/72 (26%), Positives = 32/72 (44%)
Frame = -3
Query: 347 FNNVFSTSLTPWNSMGIPLREHSYLLSVYDEVSIYGLNGTLETTVSRIILEKVDHVVKTN 168
+N + +NS+ R S L + E I+ ++ TLE+ S + DHV K
Sbjct: 684 YNTIMEVWNIKYNSLNALARLLSALYKFHPEFCIHVIDDTLESLFSAV--NNSDHVEK-Q 740
Query: 167 ERIIDSNNIGTL 132
+R+ + I L
Sbjct: 741 KRLAQARFISEL 752
>SPCC584.03c |||RanGTP-binding protein |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 551
Score = 24.6 bits (51), Expect = 8.8
Identities = 12/28 (42%), Positives = 15/28 (53%)
Frame = -3
Query: 374 ISGCEHTG*FNNVFSTSLTPWNSMGIPL 291
+S H+G F T L+PWN IPL
Sbjct: 445 VSSSRHSGIFAT--PTFLSPWNIKNIPL 470
>SPBP4H10.10 |||rhomboid family protease|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 392
Score = 24.6 bits (51), Expect = 8.8
Identities = 12/29 (41%), Positives = 17/29 (58%)
Frame = -3
Query: 332 STSLTPWNSMGIPLREHSYLLSVYDEVSI 246
S S +S +PLREH L +VY +V +
Sbjct: 50 SLSSVDVSSPSLPLREHLPLKNVYSQVPL 78
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,235,078
Number of Sequences: 5004
Number of extensions: 46602
Number of successful extensions: 165
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 158
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 163
length of database: 2,362,478
effective HSP length: 68
effective length of database: 2,022,206
effective search space used: 206265012
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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