Samia BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= S06A01NCLL0008_B22
         (606 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC167.07c ||SPAC57A7.03c|ubiquitin-protein ligase E3 |Schizosa...    49   5e-07
SPAC1805.15c |pub2||ubiquitin-protein ligase Pub2|Schizosaccharo...    39   7e-04
SPAC11G7.02 |pub1||ubiquitin-protein ligase E3|Schizosaccharomyc...    38   9e-04
SPBP8B7.27 |mug30||ubiquitin-protein ligase E3|Schizosaccharomyc...    37   0.003
SPAC12B10.01c ||SPAC31F12.02c, SPAC637.15c|ubiquitin-protein lig...    32   0.057
SPAC25H1.08c |||ribosome biogenesis protein Sqt1|Schizosaccharom...    29   0.40 
SPBC1685.06 |cid11||poly|Schizosaccharomyces pombe|chr 2|||Manual      27   2.1  
SPBC776.09 |ste13||ATP-dependent RNA helicase Ste13|Schizosaccha...    26   4.9  
SPCC1494.10 ||SPCC70.01|transcription factor |Schizosaccharomyce...    26   4.9  
SPCC18B5.08c |||isoleucine-tRNA ligase|Schizosaccharomyces pombe...    25   8.6  
SPAC20H4.04 |mfh2||ATP-dependent 3' to 5' DNA helicase |Schizosa...    25   8.6  

>SPAC167.07c ||SPAC57A7.03c|ubiquitin-protein ligase E3
            |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1029

 Score = 49.2 bits (112), Expect = 5e-07
 Identities = 26/58 (44%), Positives = 39/58 (67%), Gaps = 4/58 (6%)
 Frame = +3

Query: 45   GFQP-MPSVTIRP--ADDAHLPTANTCISRLYIPLYSSRHVLKHKLLLAIKT-KNFGF 206
            GF+  MPS  IR    D+  LPTA+TC++ L +P+YS++  L+ KLL A+++   FGF
Sbjct: 971  GFKALMPSFCIRVNGEDETRLPTASTCVNLLKLPMYSTKQTLRDKLLTAVRSGVGFGF 1028


>SPAC1805.15c |pub2||ubiquitin-protein ligase
           Pub2|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 671

 Score = 38.7 bits (86), Expect = 7e-04
 Identities = 19/39 (48%), Positives = 27/39 (69%), Gaps = 1/39 (2%)
 Frame = +3

Query: 90  AHLPTANTCISRLYIPLYSSRHVLKHKLLLAI-KTKNFG 203
           + LP A+TC +RL IP Y+S+  L+ KL +AI +T  FG
Sbjct: 631 SQLPKAHTCFNRLDIPPYNSKEELEQKLTIAIQETAGFG 669


>SPAC11G7.02 |pub1||ubiquitin-protein ligase E3|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 767

 Score = 38.3 bits (85), Expect = 9e-04
 Identities = 21/47 (44%), Positives = 31/47 (65%), Gaps = 2/47 (4%)
 Frame = +3

Query: 69  TIRPADDAH-LPTANTCISRLYIPLYSSRHVLKHKLLLAI-KTKNFG 203
           TI  A + + LP A+TC +RL +P Y+S+  L HKL +A+ +T  FG
Sbjct: 719 TIEKAGEPNKLPKAHTCFNRLDLPPYTSKKDLDHKLSIAVEETIGFG 765


>SPBP8B7.27 |mug30||ubiquitin-protein ligase E3|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 807

 Score = 36.7 bits (81), Expect = 0.003
 Identities = 29/66 (43%), Positives = 36/66 (54%), Gaps = 1/66 (1%)
 Frame = +3

Query: 12  TGSPALPAS-EEGFQPMPSVTIRPADDAHLPTANTCISRLYIPLYSSRHVLKHKLLLAIK 188
           TGS  +PA+    FQ   SV + P  D  LP ++TC + L I  YSSR  LK KL  A+ 
Sbjct: 742 TGSDRIPATGAHNFQLRISV-LGPDSD-QLPISHTCFNHLCIWEYSSREKLKKKLDTALL 799

Query: 189 TKNFGF 206
             N GF
Sbjct: 800 ETN-GF 804


>SPAC12B10.01c ||SPAC31F12.02c, SPAC637.15c|ubiquitin-protein ligase
            E3 |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1647

 Score = 32.3 bits (70), Expect = 0.057
 Identities = 24/64 (37%), Positives = 32/64 (50%), Gaps = 6/64 (9%)
 Frame = +3

Query: 12   TGSPALPASE-EGFQPMPSVTIRPAD-----DAHLPTANTCISRLYIPLYSSRHVLKHKL 173
            TGS  LP     G  P  +V  R  +     D +LP+  TC++ L +P YSS  VL  +L
Sbjct: 1574 TGSRKLPIGGFAGLNPPLTVVRRLNEPPYVPDDYLPSVMTCVNYLKLPEYSSSEVLGSRL 1633

Query: 174  LLAI 185
              AI
Sbjct: 1634 SKAI 1637


>SPAC25H1.08c |||ribosome biogenesis protein
           Sqt1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 399

 Score = 29.5 bits (63), Expect = 0.40
 Identities = 15/57 (26%), Positives = 28/57 (49%), Gaps = 2/57 (3%)
 Frame = +3

Query: 324 CICVSL--FIFHYNYLYVKFRLYVLHDQNIHDWVYLTE**YSITLCYNNTLMRWSAQ 488
           C C S+   +  Y+   +KFR  + H+Q + D  +L    Y +T C +  + +W  +
Sbjct: 300 CACASVDGTVALYDSASLKFRKSLPHEQAVIDCKFLPNTPYLLTACADCVIRKWDVR 356


>SPBC1685.06 |cid11||poly|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 478

 Score = 27.1 bits (57), Expect = 2.1
 Identities = 12/36 (33%), Positives = 20/36 (55%)
 Frame = +1

Query: 187 KLRTLVSSKFKSVILSLSFFKPSHGRLPLYSIDIDL 294
           KLRT++S++ K   L L  F  +   L +   D+D+
Sbjct: 74  KLRTILSTEIKDAKLDLFVFGSTENNLAIQQSDVDV 109


>SPBC776.09 |ste13||ATP-dependent RNA helicase
           Ste13|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 485

 Score = 25.8 bits (54), Expect = 4.9
 Identities = 13/38 (34%), Positives = 21/38 (55%)
 Frame = +1

Query: 199 LVSSKFKSVILSLSFFKPSHGRLPLYSIDIDLSNKNFV 312
           L+S +F  +I  L  + P + ++ LYS    L  KNF+
Sbjct: 198 LLSPEFTPIIEQLLSYFPKNRQISLYSATFPLIVKNFM 235


>SPCC1494.10 ||SPCC70.01|transcription factor |Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 964

 Score = 25.8 bits (54), Expect = 4.9
 Identities = 15/51 (29%), Positives = 25/51 (49%), Gaps = 4/51 (7%)
 Frame = +3

Query: 15  GSPALPASEEGFQPMPS---VTIRP-ADDAHLPTANTCISRLYIPLYSSRH 155
           G+  +P  + G  P+ S   V+++P ADD    T    +    +P Y+ RH
Sbjct: 456 GNTPIPVKQVGGMPLQSPLPVSMKPSADDHSRATPTRSVEAPTLPSYAPRH 506


>SPCC18B5.08c |||isoleucine-tRNA ligase|Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 973

 Score = 25.0 bits (52), Expect = 8.6
 Identities = 8/17 (47%), Positives = 12/17 (70%)
 Frame = -3

Query: 316 IIQNFCLINLYQWNTAA 266
           +   FCL NL+ WNT++
Sbjct: 689 LTSRFCLGNLHDWNTSS 705


>SPAC20H4.04 |mfh2||ATP-dependent 3' to 5' DNA helicase
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 783

 Score = 25.0 bits (52), Expect = 8.6
 Identities = 11/41 (26%), Positives = 23/41 (56%)
 Frame = +3

Query: 285 YRFIKQKFCIILDCICVSLFIFHYNYLYVKFRLYVLHDQNI 407
           + F++QK  I ++C C+++  +    L+V  R Y    +N+
Sbjct: 332 FNFLRQKKLIPINCECLNIKAY---TLFVSLRKYSFSSKNV 369


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,529,676
Number of Sequences: 5004
Number of extensions: 53285
Number of successful extensions: 123
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 121
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 123
length of database: 2,362,478
effective HSP length: 69
effective length of database: 2,017,202
effective search space used: 266270664
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)