Samia BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= S06A01NCLL0008_B07
         (527 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC1687.22c |puf3|SPAC222.02c|RNA-binding protein Puf3 |Schizos...    74   2e-14
SPAC4G9.05 |mpf1||meiotic PUF family protein 1|Schizosaccharomyc...    49   5e-07
SPAC6G9.14 |||RNA-binding protein|Schizosaccharomyces pombe|chr ...    47   2e-06
SPAC4G8.03c |||RNA-binding protein|Schizosaccharomyces pombe|chr...    46   5e-06
SPCC1682.08c |||RNA-binding protein Mcp2|Schizosaccharomyces pom...    45   6e-06
SPBP35G2.14 |||RNA-binding protein|Schizosaccharomyces pombe|chr...    29   0.32 
SPCP1E11.11 |||Puf family RNA-binding protein|Schizosaccharomyce...    27   1.3  
SPCC663.10 |||methyltransferase, DUF1613 family |Schizosaccharom...    26   4.0  
SPAC806.08c |mod21||gamma tubulin complex subunit Mod21|Schizosa...    25   5.3  
SPCC1840.02c |bgs4|orb11, cwg1|1,3-beta-glucan synthase subunit ...    25   7.0  

>SPAC1687.22c |puf3|SPAC222.02c|RNA-binding protein Puf3
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 732

 Score = 73.7 bits (173), Expect = 2e-14
 Identities = 31/69 (44%), Positives = 49/69 (71%)
 Frame = +1

Query: 67  NDNREFFSALHVMMKDQYANYVVQKMIDVAEPTQRKVLMHKIRPHVGSLRKYTYGKHIIA 246
           ++  E  S L +MMKD+YANYV+QK++D +   +R +L+  I PH+  L+K+TYGKH+I 
Sbjct: 651 SEKAENCSILMLMMKDKYANYVIQKLLDASPEEERDLLISYIYPHISVLKKFTYGKHLIM 710

Query: 247 KLEKFFMKA 273
            +E+F  K+
Sbjct: 711 SVERFRQKS 719



 Score = 42.7 bits (96), Expect = 3e-05
 Identities = 21/54 (38%), Positives = 32/54 (59%)
 Frame = +1

Query: 88  SALHVMMKDQYANYVVQKMIDVAEPTQRKVLMHKIRPHVGSLRKYTYGKHIIAK 249
           S L +MM D + NYVVQK  +     Q+++L+ +I+ HV SL    YG  ++ K
Sbjct: 437 SCLQLMM-DIFGNYVVQKYFEFGNEKQKQILLSQIKGHVFSLSLQMYGCRVVQK 489



 Score = 33.1 bits (72), Expect = 0.026
 Identities = 13/50 (26%), Positives = 30/50 (60%)
 Frame = +1

Query: 94  LHVMMKDQYANYVVQKMIDVAEPTQRKVLMHKIRPHVGSLRKYTYGKHII 243
           +HV+    Y   V+Q+ I+    ++RK+++ ++ PH+  L +  YG +++
Sbjct: 546 IHVLSAHPYGCRVIQRAIEHCH-SERKLIIEELLPHILKLTQDQYGNYVV 594



 Score = 32.7 bits (71), Expect = 0.035
 Identities = 13/46 (28%), Positives = 25/46 (54%)
 Frame = +1

Query: 112 DQYANYVVQKMIDVAEPTQRKVLMHKIRPHVGSLRKYTYGKHIIAK 249
           DQ  N+V+QK I+  +    + ++  +RP +  L  + YG  +I +
Sbjct: 516 DQNGNHVIQKAIECIDTGHLQFILRALRPQIHVLSAHPYGCRVIQR 561



 Score = 29.5 bits (63), Expect = 0.32
 Identities = 11/49 (22%), Positives = 26/49 (53%)
 Frame = +1

Query: 103 MMKDQYANYVVQKMIDVAEPTQRKVLMHKIRPHVGSLRKYTYGKHIIAK 249
           + +DQY NYVVQ ++     + +K +   +  H+  L  + +  +++ +
Sbjct: 584 LTQDQYGNYVVQHILRTGSESDKKYIFDLMIDHLLFLSCHKFASNVVER 632


>SPAC4G9.05 |mpf1||meiotic PUF family protein 1|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 581

 Score = 48.8 bits (111), Expect = 5e-07
 Identities = 21/55 (38%), Positives = 35/55 (63%)
 Frame = +1

Query: 94  LHVMMKDQYANYVVQKMIDVAEPTQRKVLMHKIRPHVGSLRKYTYGKHIIAKLEK 258
           L  +  +QY NY+VQ ++ VA P+Q  ++   ++ H+ SLR + YG+ I A +EK
Sbjct: 524 LQEIASNQYGNYIVQYLLQVATPSQINLMAEHLKKHMVSLRGHKYGQRIAALVEK 578



 Score = 34.3 bits (75), Expect = 0.011
 Identities = 12/49 (24%), Positives = 28/49 (57%)
 Frame = +1

Query: 103 MMKDQYANYVVQKMIDVAEPTQRKVLMHKIRPHVGSLRKYTYGKHIIAK 249
           +MK+++ N+++QK  + +   Q +   + ++ HV  L    +G H++ K
Sbjct: 299 LMKNKFGNFLIQKCFEYSTEAQLQSFSYFLKKHVKELSIDAFGSHVLQK 347


>SPAC6G9.14 |||RNA-binding protein|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 681

 Score = 47.2 bits (107), Expect = 2e-06
 Identities = 19/52 (36%), Positives = 35/52 (67%)
 Frame = +1

Query: 103 MMKDQYANYVVQKMIDVAEPTQRKVLMHKIRPHVGSLRKYTYGKHIIAKLEK 258
           +++D +ANYV+Q  +D A   QR  L+ +I+P + S++    G+ I++KLE+
Sbjct: 606 LLRDSFANYVIQTALDNASVKQRAELVERIKPLIPSIKNTPCGRRILSKLER 657



 Score = 33.1 bits (72), Expect = 0.026
 Identities = 19/52 (36%), Positives = 30/52 (57%), Gaps = 2/52 (3%)
 Frame = +1

Query: 112 DQYANYVVQKMID-VAEPTQRKVLMHKIRPHVGSLRKYTYGKHIIAK-LEKF 261
           + +    +QK+ID V+ P Q   +++ +RP+V  L K   G H+I K L KF
Sbjct: 426 NMHGTRALQKIIDLVSSPDQISCIVNALRPNVVLLTKDLNGNHVIQKCLNKF 477



 Score = 31.9 bits (69), Expect = 0.061
 Identities = 15/53 (28%), Positives = 26/53 (49%)
 Frame = +1

Query: 94  LHVMMKDQYANYVVQKMIDVAEPTQRKVLMHKIRPHVGSLRKYTYGKHIIAKL 252
           L V+M D + NY+ QK+ + A   QR   +  I P +  +    +G   + K+
Sbjct: 384 LAVLMVDAFGNYLCQKLFEHASEAQRSTFIQIIAPKLVPISFNMHGTRALQKI 436



 Score = 28.7 bits (61), Expect = 0.57
 Identities = 13/38 (34%), Positives = 22/38 (57%)
 Frame = +1

Query: 130 VVQKMIDVAEPTQRKVLMHKIRPHVGSLRKYTYGKHII 243
           VVQ+  D A P Q + L+  I PH  +L +  +G +++
Sbjct: 505 VVQRCFDHASPAQIEQLVEHIVPHALTLVQDAFGNYVL 542


>SPAC4G8.03c |||RNA-binding protein|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 780

 Score = 45.6 bits (103), Expect = 5e-06
 Identities = 18/56 (32%), Positives = 37/56 (66%)
 Frame = +1

Query: 85  FSALHVMMKDQYANYVVQKMIDVAEPTQRKVLMHKIRPHVGSLRKYTYGKHIIAKL 252
           F  +  ++ D YANYV+Q+ ++VA+ +Q+ +++  I   +  ++   +G+HI+AKL
Sbjct: 719 FPNIEQLLDDCYANYVMQRFLNVADESQKFLILRSISHVIPKIQNTRHGRHILAKL 774



 Score = 28.7 bits (61), Expect = 0.57
 Identities = 16/57 (28%), Positives = 25/57 (43%)
 Frame = +1

Query: 82  FFSALHVMMKDQYANYVVQKMIDVAEPTQRKVLMHKIRPHVGSLRKYTYGKHIIAKL 252
           F   L  + KDQY    +QK++D        +   +IR  V  L    +G ++  KL
Sbjct: 463 FLGHLSTICKDQYGCRYLQKLLDENPKVNASLFFPEIRQSVVQLMIDPFGNYMCQKL 519


>SPCC1682.08c |||RNA-binding protein Mcp2|Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 703

 Score = 45.2 bits (102), Expect = 6e-06
 Identities = 17/50 (34%), Positives = 34/50 (68%)
 Frame = +1

Query: 112 DQYANYVVQKMIDVAEPTQRKVLMHKIRPHVGSLRKYTYGKHIIAKLEKF 261
           DQY NY++Q++I + +P ++ +++  I+ H+ SLR   YG+ +   +EK+
Sbjct: 636 DQYGNYLIQQIIQLGQPAEKNLVITHIKKHMVSLRGSKYGQKVAYLVEKW 685


>SPBP35G2.14 |||RNA-binding protein|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 1060

 Score = 29.5 bits (63), Expect = 0.32
 Identities = 12/50 (24%), Positives = 24/50 (48%)
 Frame = +1

Query: 103 MMKDQYANYVVQKMIDVAEPTQRKVLMHKIRPHVGSLRKYTYGKHIIAKL 252
           +  D   N VVQK+ D      +++++ +I PH+  +  +  G     K+
Sbjct: 776 LSSDHLGNTVVQKLFDYCSDPVKEMMLERIAPHLAQIGIHKNGTWAAQKI 825


>SPCP1E11.11 |||Puf family RNA-binding protein|Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 642

 Score = 27.5 bits (58), Expect = 1.3
 Identities = 12/38 (31%), Positives = 22/38 (57%)
 Frame = +1

Query: 130 VVQKMIDVAEPTQRKVLMHKIRPHVGSLRKYTYGKHII 243
           V  K+  +A    RKV++  +RP++    K +YG H++
Sbjct: 363 VAMKLFALANAKDRKVMLKSLRPYLIETAKDSYG-HLV 399


>SPCC663.10 |||methyltransferase, DUF1613 family
           |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 502

 Score = 25.8 bits (54), Expect = 4.0
 Identities = 14/54 (25%), Positives = 25/54 (46%)
 Frame = +1

Query: 76  REFFSALHVMMKDQYANYVVQKMIDVAEPTQRKVLMHKIRPHVGSLRKYTYGKH 237
           R  F   +V++KD+YA  +V   ++  +P +       I   +  L K TY  +
Sbjct: 221 RNKFQDTYVILKDKYAKQLVDNWVEKTDPGKHVFEDLAIAAFLIELWKQTYSSN 274


>SPAC806.08c |mod21||gamma tubulin complex subunit
           Mod21|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 618

 Score = 25.4 bits (53), Expect = 5.3
 Identities = 21/84 (25%), Positives = 36/84 (42%), Gaps = 1/84 (1%)
 Frame = +1

Query: 13  HHATRNERALLIDELCGFNDNREFFSALHVMMKDQYANYVVQKMIDVAEPTQRKVLMH-K 189
           HH +      ++ EL  +  N EFF        D +     Q   +  EP    V ++ +
Sbjct: 205 HHMSEEILRSIMTELADYGSNLEFFRQKLTSPSDSFFKSDDQSTKN--EPFLSVVFIYPQ 262

Query: 190 IRPHVGSLRKYTYGKHIIAKLEKF 261
           I+PH+ S  K T+ + I  + E +
Sbjct: 263 IKPHLLSCLKNTHQELIKLETEVY 286


>SPCC1840.02c |bgs4|orb11, cwg1|1,3-beta-glucan synthase subunit
            Bgs4|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 1955

 Score = 25.0 bits (52), Expect = 7.0
 Identities = 13/29 (44%), Positives = 16/29 (55%), Gaps = 1/29 (3%)
 Frame = -1

Query: 428  CTITNIHIM-YKRRGEGHPRRRISLLVPR 345
            C +T   I  YKRR  G P  +IS+  PR
Sbjct: 1588 CRLTRTRITGYKRRVLGQPSDKISMDTPR 1616


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,895,956
Number of Sequences: 5004
Number of extensions: 34840
Number of successful extensions: 89
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 76
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 89
length of database: 2,362,478
effective HSP length: 68
effective length of database: 2,022,206
effective search space used: 216376042
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)