BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= S06A01NCLL0006_I18
(531 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC23A1.12c |||phenylalanine-tRNA ligase beta subunit |Schizosa... 28 1.00
SPAC22F3.12c |rgs1||regulator of G-protein signaling Rgs1|Schizo... 27 1.3
SPCC132.03 |||sequence orphan|Schizosaccharomyces pombe|chr 3|||... 26 4.0
SPCC74.06 |mak3|phk2|histidine kinase Mak3 |Schizosaccharomyces ... 25 7.0
SPBC1271.10c |||membrane transporter|Schizosaccharomyces pombe|c... 25 9.3
SPAC22F3.10c |gcs1|apd1|glutamate-cysteine ligase Gcs1 |Schizosa... 25 9.3
SPBC713.03 |||D-lactate dehydrogenase |Schizosaccharomyces pombe... 25 9.3
>SPAC23A1.12c |||phenylalanine-tRNA ligase beta subunit
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 589
Score = 27.9 bits (59), Expect = 1.00
Identities = 15/43 (34%), Positives = 23/43 (53%)
Frame = -2
Query: 266 LEDVTVKYCFELEKHIYPANSSLGYGCESHQLRTCDSIRQRTA 138
+ED+ + Y ++ KH YPA+ S+ +G R D IR A
Sbjct: 354 MEDLGIAYGYDNLKHTYPAH-SVTFGKPFEVNRLADIIRNEVA 395
>SPAC22F3.12c |rgs1||regulator of G-protein signaling
Rgs1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 481
Score = 27.5 bits (58), Expect = 1.3
Identities = 11/32 (34%), Positives = 18/32 (56%)
Frame = -3
Query: 418 LVSKYCLHNDHSESPVLHVIKNKHSGPIDVYV 323
+VS++ HN H+ L+ K HS P+ V +
Sbjct: 161 VVSEFLQHNGHNSQAELYASKWNHSAPVIVSI 192
>SPCC132.03 |||sequence orphan|Schizosaccharomyces pombe|chr
3|||Manual
Length = 176
Score = 25.8 bits (54), Expect = 4.0
Identities = 10/16 (62%), Positives = 13/16 (81%)
Frame = +3
Query: 309 VQVPIT*TSMGPLCLF 356
+Q PIT T MGP+CL+
Sbjct: 85 LQKPITCTPMGPICLW 100
>SPCC74.06 |mak3|phk2|histidine kinase Mak3 |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 2344
Score = 25.0 bits (52), Expect = 7.0
Identities = 11/30 (36%), Positives = 18/30 (60%)
Frame = -3
Query: 463 NSLKVTIEVYT*KKKLVSKYCLHNDHSESP 374
N LKVT++V+ + + Y N++ ESP
Sbjct: 2166 NFLKVTLQVFKSNEDTCNFYSYVNEYGESP 2195
>SPBC1271.10c |||membrane transporter|Schizosaccharomyces pombe|chr
2|||Manual
Length = 581
Score = 24.6 bits (51), Expect = 9.3
Identities = 10/42 (23%), Positives = 21/42 (50%)
Frame = -3
Query: 193 TDASHISCVRATASANVPHSQIQPVLDKHFHWLALITVYPSN 68
+DA S ++ + N + +QP+ K +W + + P+N
Sbjct: 279 SDAMDDSKMKVDYTVNEISNAVQPIYSKPSYWKRIALITPAN 320
>SPAC22F3.10c |gcs1|apd1|glutamate-cysteine ligase Gcs1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 669
Score = 24.6 bits (51), Expect = 9.3
Identities = 13/42 (30%), Positives = 19/42 (45%), Gaps = 4/42 (9%)
Frame = -2
Query: 263 EDVTVKYCFELEKHIYPANSSLGYGC----ESHQLRTCDSIR 150
ED + L+ HIY + G GC + Q ++CD R
Sbjct: 285 EDANARDGAALDNHIYMDSMGFGMGCCCLQITFQAKSCDEAR 326
>SPBC713.03 |||D-lactate dehydrogenase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 526
Score = 24.6 bits (51), Expect = 9.3
Identities = 16/46 (34%), Positives = 24/46 (52%)
Frame = -3
Query: 478 DFSLPNSLKVTIEVYT*KKKLVSKYCLHNDHSESPVLHVIKNKHSG 341
D SLP L V ++ KK + ++ L +D E PV+ V+ H G
Sbjct: 402 DVSLP--LPVLYDLVNDTKKRLIEFNLLDDTPEHPVIDVVGFGHMG 445
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,236,818
Number of Sequences: 5004
Number of extensions: 46221
Number of successful extensions: 122
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 119
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 122
length of database: 2,362,478
effective HSP length: 68
effective length of database: 2,022,206
effective search space used: 218398248
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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