Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= S06A01NCLL0005_N05
(460 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC4A8.08c |vas1||mitochondrial valine-tRNA ligase Vas1|Schizos... 96 2e-21
SPBC1709.02c |vas2|SPBC1734.18c|valine-tRNA ligase Vas2 |Schizos... 95 5e-21
SPBC8D2.06 |||isoleucine-tRNA ligase |Schizosaccharomyces pombe|... 45 6e-06
SPCC18B5.08c |||isoleucine-tRNA ligase|Schizosaccharomyces pombe... 39 3e-04
SPAC4G8.09 |||mitochondrial leucine-tRNA ligase|Schizosaccharomy... 32 0.048
SPBC2D10.14c |myo51||myosin type V|Schizosaccharomyces pombe|chr... 26 2.4
SPAC3F10.11c |abc2||glutathione S-conjugate-exporting ATPase Abc... 26 3.2
SPAC688.08 |srb8|med12|mediator complex subunit Srb8 |Schizosacc... 25 4.2
SPCC777.15 |||tRNA dihydrouridine synthase Dus4 |Schizosaccharom... 25 4.2
SPAC2C4.07c |||ribonuclease II |Schizosaccharomyces pombe|chr 1|... 25 5.6
SPAC1327.01c ||SPAC1783.09c, SPAC18G6.16c|transcription factor, ... 25 7.3
SPBC16C6.10 |chp2||chromodomain protein 2|Schizosaccharomyces po... 25 7.3
SPAPB8E5.04c |||phosphatidylglycerol/phosphatidylinositol transf... 25 7.3
SPBC17A3.04c |||methionine-tRNA ligase |Schizosaccharomyces pomb... 25 7.3
SPAC2G11.07c |ptc3||protein phosphatase 2C Ptc3|Schizosaccharomy... 24 9.7
SPCC1235.04c |||FAD synthetase|Schizosaccharomyces pombe|chr 3||... 24 9.7
>SPAC4A8.08c |vas1||mitochondrial valine-tRNA ligase
Vas1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 950
Score = 96.3 bits (229), Expect = 2e-21
Identities = 47/106 (44%), Positives = 63/106 (59%), Gaps = 1/106 (0%)
Frame = +2
Query: 125 PHAMTLPICSRSGDVIEHLPKEQWFLSCSDMNERAANLVKNNSLKIQPDRFIKHWLDWSK 304
PH++ L +CSR+GDVIE + QW+LS + + L +N LK+ P K W W K
Sbjct: 375 PHSLILSVCSRTGDVIEPVMVPQWYLSVDSLKKEV--LKSSNKLKLVPSLARKEWDSWFK 432
Query: 305 DDKDWCISRQLWWGHQIPAYKCNANKDIIWIAA-NDETSAKLGASK 439
DWCISRQ+WWGHQIP +K ++ WIAA N E + +L K
Sbjct: 433 KMGDWCISRQIWWGHQIPVWK--ILEEDKWIAAPNYEKALQLSVGK 476
>SPBC1709.02c |vas2|SPBC1734.18c|valine-tRNA ligase Vas2
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 980
Score = 95.1 bits (226), Expect = 5e-21
Identities = 40/115 (34%), Positives = 64/115 (55%)
Frame = +2
Query: 29 ENVENFDNYQRYDCRQMXXXXXXXXXXXXXXXPHAMTLPICSRSGDVIEHLPKEQWFLSC 208
EN F +R+ R + M +P+C ++ D+IE + K QW+++
Sbjct: 417 ENCGEFAGMKRFTARVKVVERLKELGLFVGTKENPMVIPLCGKTSDIIEPVMKPQWWVNQ 476
Query: 209 SDMNERAANLVKNNSLKIQPDRFIKHWLDWSKDDKDWCISRQLWWGHQIPAYKCN 373
+M AA +VK+ ++I PD + ++ W ++ +DWCISRQLWWGH+IPAY N
Sbjct: 477 KEMAAAAAEVVKSGEIEIAPDMSRREFIRWMENIQDWCISRQLWWGHRIPAYFVN 531
>SPBC8D2.06 |||isoleucine-tRNA ligase |Schizosaccharomyces pombe|chr
2|||Manual
Length = 1064
Score = 44.8 bits (101), Expect = 6e-06
Identities = 27/99 (27%), Positives = 48/99 (48%), Gaps = 1/99 (1%)
Frame = +2
Query: 143 PICSRSGDVIEHLPKEQWFLSCSDM-NERAANLVKNNSLKIQPDRFIKHWLDWSKDDKDW 319
P C RS + + WF+ ++ NE N++ + + Q R K + +W K+ +DW
Sbjct: 394 PFCWRSDTPLIYRAVPSWFVRVKEITNEMVENVMSTHWVP-QNIRD-KRFANWLKNARDW 451
Query: 320 CISRQLWWGHQIPAYKCNANKDIIWIAANDETSAKLGAS 436
ISR +WG IP + + ++++ I + E G S
Sbjct: 452 NISRNRYWGTPIPLWVSDDYEEVVCIGSIKELEELSGVS 490
>SPCC18B5.08c |||isoleucine-tRNA ligase|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 973
Score = 39.1 bits (87), Expect = 3e-04
Identities = 21/56 (37%), Positives = 28/56 (50%)
Frame = +2
Query: 191 QWFLSCSDMNERAANLVKNNSLKIQPDRFIKHWLDWSKDDKDWCISRQLWWGHQIP 358
QWF+S NER + +S+K+ P L + +WCISRQ WG IP
Sbjct: 441 QWFISLE--NERKTAIKALDSVKMIPPNSRARLLGFLNGRPEWCISRQRAWGLPIP 494
>SPAC4G8.09 |||mitochondrial leucine-tRNA ligase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 874
Score = 31.9 bits (69), Expect = 0.048
Identities = 12/20 (60%), Positives = 13/20 (65%)
Frame = +2
Query: 311 KDWCISRQLWWGHQIPAYKC 370
KDW ISRQ +WG IP C
Sbjct: 428 KDWLISRQRFWGTPIPMVHC 447
Score = 28.3 bits (60), Expect = 0.60
Identities = 15/48 (31%), Positives = 26/48 (54%)
Frame = +2
Query: 155 RSGDVIEHLPKEQWFLSCSDMNERAANLVKNNSLKIQPDRFIKHWLDW 298
RSG ++E QWFL SD +++ + ++ +L PD+ K +W
Sbjct: 204 RSGAIVEKKNLRQWFLKISDYSDQLLDDLE--TLPKWPDKVKKMQRNW 249
>SPBC2D10.14c |myo51||myosin type V|Schizosaccharomyces pombe|chr
2|||Manual
Length = 1471
Score = 26.2 bits (55), Expect = 2.4
Identities = 15/56 (26%), Positives = 23/56 (41%), Gaps = 4/56 (7%)
Frame = +2
Query: 173 EHLPKEQWFLSCSDM-NERAANLVKNNSLKIQPDRFIKHWLDW---SKDDKDWCIS 328
EH K C + NE+ + K++ + ++K LDW D CIS
Sbjct: 444 EHFEKNSMEQFCINYANEKLQQEFNKHVFKLEQEEYVKEGLDWRLIEYSDNQGCIS 499
>SPAC3F10.11c |abc2||glutathione S-conjugate-exporting ATPase
Abc2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1463
Score = 25.8 bits (54), Expect = 3.2
Identities = 11/15 (73%), Positives = 11/15 (73%)
Frame = -3
Query: 347 GPTTIVLIYTNLYHL 303
GP IVL TNLYHL
Sbjct: 379 GPFQIVLALTNLYHL 393
>SPAC688.08 |srb8|med12|mediator complex subunit Srb8
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1233
Score = 25.4 bits (53), Expect = 4.2
Identities = 11/34 (32%), Positives = 19/34 (55%)
Frame = +2
Query: 194 WFLSCSDMNERAANLVKNNSLKIQPDRFIKHWLD 295
WF+ C+ +NE + L K + I ++K+W D
Sbjct: 180 WFIRCAGVNEARSFLRKVQTTDI--TEWVKNWTD 211
>SPCC777.15 |||tRNA dihydrouridine synthase Dus4
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 326
Score = 25.4 bits (53), Expect = 4.2
Identities = 10/15 (66%), Positives = 12/15 (80%)
Frame = -1
Query: 313 FIIFRPIEPMFDESV 269
F++ RP EPMF ESV
Sbjct: 306 FVVRRPDEPMFGESV 320
>SPAC2C4.07c |||ribonuclease II |Schizosaccharomyces pombe|chr
1|||Manual
Length = 927
Score = 25.0 bits (52), Expect = 5.6
Identities = 11/30 (36%), Positives = 18/30 (60%)
Frame = +3
Query: 270 TDSSNIGSIGLKMIKIGVYQDNCGGAIKFL 359
T+ SN G++ +KI + D GGA++ L
Sbjct: 247 TEKSNFLQNGVEKVKIKDHDDELGGAMEHL 276
>SPAC1327.01c ||SPAC1783.09c, SPAC18G6.16c|transcription factor,
zf-fungal binuclear cluster type |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 977
Score = 24.6 bits (51), Expect = 7.3
Identities = 12/30 (40%), Positives = 16/30 (53%)
Frame = -2
Query: 258 FNELFLTRFAALSFISLHDKNHCSFGRCSM 169
F+ L+ AAL +SLHD C G+ M
Sbjct: 486 FDRPDLSLVAALLLLSLHDSGTCETGKSWM 515
>SPBC16C6.10 |chp2||chromodomain protein 2|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 380
Score = 24.6 bits (51), Expect = 7.3
Identities = 10/22 (45%), Positives = 12/22 (54%)
Frame = +3
Query: 24 RWKTSRTSTIINVTIADKCSLK 89
+WK ST N+ I KC LK
Sbjct: 347 KWKNGYVSTHDNIIIHQKCPLK 368
>SPAPB8E5.04c |||phosphatidylglycerol/phosphatidylinositol transfer
protein |Schizosaccharomyces pombe|chr 1|||Manual
Length = 188
Score = 24.6 bits (51), Expect = 7.3
Identities = 12/39 (30%), Positives = 18/39 (46%)
Frame = +2
Query: 194 WFLSCSDMNERAANLVKNNSLKIQPDRFIKHWLDWSKDD 310
WF S S ++ +LV + S KI + DW + D
Sbjct: 22 WFSSFSFGESKSTDLVSSTSEKIPGANPASYCADWDRGD 60
>SPBC17A3.04c |||methionine-tRNA ligase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 782
Score = 24.6 bits (51), Expect = 7.3
Identities = 11/37 (29%), Positives = 17/37 (45%), Gaps = 1/37 (2%)
Frame = +2
Query: 296 WSKDD-KDWCISRQLWWGHQIPAYKCNANKDIIWIAA 403
W K+ + CI+R L WG +P + +W A
Sbjct: 443 WLKEGLRPRCITRDLKWGTPVPLEEFKGKVLYVWFDA 479
>SPAC2G11.07c |ptc3||protein phosphatase 2C Ptc3|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 414
Score = 24.2 bits (50), Expect = 9.7
Identities = 13/40 (32%), Positives = 20/40 (50%)
Frame = +3
Query: 180 YQRNSGSYRAVI*TKGLQILLKITH*KSNLTDSSNIGSIG 299
Y N+G R V+ +KG+ L H SN + + I + G
Sbjct: 133 YCANAGDSRTVLGSKGIAKPLSADHKPSNEAEKARICAAG 172
>SPCC1235.04c |||FAD synthetase|Schizosaccharomyces pombe|chr
3|||Manual
Length = 265
Score = 24.2 bits (50), Expect = 9.7
Identities = 12/37 (32%), Positives = 20/37 (54%), Gaps = 1/37 (2%)
Frame = -2
Query: 264 WIFNELF-LTRFAALSFISLHDKNHCSFGRCSMTSPD 157
W + E++ L + SL+D+ + S G S TSP+
Sbjct: 175 WSYTEIWDLLLETNTKYCSLYDRGYTSLGGVSDTSPN 211
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,816,208
Number of Sequences: 5004
Number of extensions: 36346
Number of successful extensions: 91
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 88
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 90
length of database: 2,362,478
effective HSP length: 67
effective length of database: 2,027,210
effective search space used: 172312850
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
- SilkBase 1999-2023 -