Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= S06A01NCLL0005_D10
(528 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 237 1e-63
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 135 3e-33
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 130 9e-32
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 128 7e-31
SPBC19G7.16 |iws1||transcription elongation factor complex subun... 27 1.7
SPAC6C3.08 |||gankyrin|Schizosaccharomyces pombe|chr 1|||Manual 27 1.7
SPAC29E6.01 |pof11|SPAC30.05, mug156|F-box protein Pof11|Schizos... 27 2.3
SPAC8F11.09c |nnt1||nicotinamide N-methyltransferase Nnt1 |Schiz... 25 5.3
SPAC23A1.19c ||SPAC26H5.01c|RecQ type DNA helicase Hrq1 |Schizos... 25 7.0
SPAC29B12.01 |ino80|SPAC3G6.12|SNF2 family helicase Ino80|Schizo... 25 7.0
SPAC27D7.03c |mei2||RNA-binding protein involved in meiosis Mei2... 25 9.2
SPBC3B8.04c |||membrane transporter|Schizosaccharomyces pombe|ch... 25 9.2
SPAC1D4.11c |lkh1|kic1|dual specificity protein kinase Lkh1|Schi... 25 9.2
>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 448
Score = 237 bits (579), Expect = 1e-63
Identities = 101/134 (75%), Positives = 118/134 (88%)
Frame = +2
Query: 71 MREIVHIQAGQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERINVYYNEASGGKYV 250
MREIVHIQAGQCGNQ+GA FW I+DEHG+D G YHG S+ Q ER+NVY+NEA+GGKYV
Sbjct: 1 MREIVHIQAGQCGNQVGAAFWSTIADEHGLDSAGIYHGTSEAQHERLNVYFNEAAGGKYV 60
Query: 251 PRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWANGHYTEGAELVDSVLDVV 430
PRA+LVDLEPGTMD+V+SG FG +FRPDN ++GQSGAGN WA GHYTEGAEL D+VLDVV
Sbjct: 61 PRAVLVDLEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGAELADAVLDVV 120
Query: 431 RKESESCDCLQGFQ 472
R+E+E+CD LQGFQ
Sbjct: 121 RREAEACDALQGFQ 134
>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 449
Score = 135 bits (327), Expect = 3e-33
Identities = 62/135 (45%), Positives = 85/135 (62%), Gaps = 2/135 (1%)
Frame = +2
Query: 71 MREIVHIQAGQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLER--INVYYNEASGGK 244
MREI+ I GQ G QIG WE+ EHGI P G + ++ Q + +++E GK
Sbjct: 1 MREIISIHVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGK 60
Query: 245 YVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWANGHYTEGAELVDSVLD 424
YVPR+I VDLEP +D VR+GP+ +F P+ + G+ A NN+A GHYT G ELVD V D
Sbjct: 61 YVPRSIYVDLEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHYTVGKELVDEVTD 120
Query: 425 VVRKESESCDCLQGF 469
+R+ +++C LQGF
Sbjct: 121 KIRRIADNCSGLQGF 135
>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 455
Score = 130 bits (315), Expect = 9e-32
Identities = 59/139 (42%), Positives = 86/139 (61%), Gaps = 6/139 (4%)
Frame = +2
Query: 71 MREIVHIQAGQCGNQIGAKFWEIISDEHGIDPTG------AYHGDSDLQLERINVYYNEA 232
MRE++ + GQ G QIG WE+ EHGI P G H ++ + +++E
Sbjct: 1 MREVISVHVGQAGVQIGNACWELYCLEHGIGPDGFPTENSEVHKNNSYLNDGFGTFFSET 60
Query: 233 SGGKYVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWANGHYTEGAELVD 412
GK+VPR+I VDLEP +D VR+GP+ +F P+ V G+ A NN+A GHYT G E++D
Sbjct: 61 GQGKFVPRSIYVDLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHYTVGKEMID 120
Query: 413 SVLDVVRKESESCDCLQGF 469
SVL+ +R+ +++C LQGF
Sbjct: 121 SVLERIRRMADNCSGLQGF 139
>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 446
Score = 128 bits (308), Expect = 7e-31
Identities = 53/134 (39%), Positives = 92/134 (68%), Gaps = 2/134 (1%)
Frame = +2
Query: 74 REIVHIQAGQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERINVYYNEASGGKYVP 253
REI+ +QAGQCGNQIG++FW+ + EHGI P G + ++R +V++ ++ +Y+P
Sbjct: 3 REIITLQAGQCGNQIGSQFWQQLCLEHGIGPDGTLESFATEGVDRKDVFFYQSDDTRYIP 62
Query: 254 RAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQS--GAGNNWANGHYTEGAELVDSVLDV 427
RAIL+DLEP ++++ S +G ++ P+N + ++ GAGNNWANG Y+ + + ++D+
Sbjct: 63 RAILIDLEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNWANG-YSHAERIFEDIMDM 121
Query: 428 VRKESESCDCLQGF 469
+ +E++ D L+GF
Sbjct: 122 IDREADGSDSLEGF 135
>SPBC19G7.16 |iws1||transcription elongation factor complex subunit
Iws1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 428
Score = 27.1 bits (57), Expect = 1.7
Identities = 10/34 (29%), Positives = 22/34 (64%)
Frame = +2
Query: 110 NQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERI 211
N++G E+++++ +DPT A + DLQ++ +
Sbjct: 133 NELGENEEEVLTEQKQLDPTLAAKKELDLQMDAV 166
>SPAC6C3.08 |||gankyrin|Schizosaccharomyces pombe|chr 1|||Manual
Length = 234
Score = 27.1 bits (57), Expect = 1.7
Identities = 13/30 (43%), Positives = 18/30 (60%)
Frame = +2
Query: 374 ANGHYTEGAELVDSVLDVVRKESESCDCLQ 463
A GH G ELV + D +RK+SE+ L+
Sbjct: 183 AEGHPDVGVELVRAGADTLRKDSENHTALE 212
>SPAC29E6.01 |pof11|SPAC30.05, mug156|F-box protein
Pof11|Schizosaccharomyces pombe|chr 1|||Manual
Length = 506
Score = 26.6 bits (56), Expect = 2.3
Identities = 14/35 (40%), Positives = 19/35 (54%), Gaps = 1/35 (2%)
Frame = +2
Query: 305 GPFGQIFRPDNFVFGQSGAG-NNWANGHYTEGAEL 406
GP+G +F P F+F +G NW+ Y E A L
Sbjct: 157 GPYGTMFLPQQFIFDSNGRPLLNWSY-LYKEHAHL 190
>SPAC8F11.09c |nnt1||nicotinamide N-methyltransferase Nnt1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 255
Score = 25.4 bits (53), Expect = 5.3
Identities = 13/32 (40%), Positives = 16/32 (50%)
Frame = -1
Query: 363 PAPDCPKTKLSGRNICPKGPERTESMVPGSRS 268
P+ PK L R I P GPE + + GS S
Sbjct: 20 PSTPPPKEVLHTRVIVPNGPEEIKLRLVGSHS 51
>SPAC23A1.19c ||SPAC26H5.01c|RecQ type DNA helicase Hrq1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1063
Score = 25.0 bits (52), Expect = 7.0
Identities = 12/44 (27%), Positives = 20/44 (45%)
Frame = +2
Query: 326 RPDNFVFGQSGAGNNWANGHYTEGAELVDSVLDVVRKESESCDC 457
RP +F G++ G + E D ++ + + ESCDC
Sbjct: 955 RPSRLIF-YDNCGDSSGAGLCNKAYEHTDELITMAIERIESCDC 997
>SPAC29B12.01 |ino80|SPAC3G6.12|SNF2 family helicase
Ino80|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1604
Score = 25.0 bits (52), Expect = 7.0
Identities = 11/36 (30%), Positives = 19/36 (52%)
Frame = -2
Query: 437 LFGLRQELSRLAQRPLCSVHWPSCYRRRTVRKQSCQ 330
L +QE++R + C +W S R+ +RK C+
Sbjct: 903 LHNWQQEITRFVPKLKCIPYWGSTKDRKILRKFWCR 938
>SPAC27D7.03c |mei2||RNA-binding protein involved in meiosis
Mei2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 750
Score = 24.6 bits (51), Expect = 9.2
Identities = 17/67 (25%), Positives = 32/67 (47%), Gaps = 1/67 (1%)
Frame = +3
Query: 312 SDRYSALTTLFSDSPAPVTTGPMDTTQRALS*STQFLT*SEK-NPNHVIVYKDSNSHTLS 488
++ Y +TL P+P+ + T+ + S S +LT ++ PN V + N++ S
Sbjct: 45 NEHYPPKSTLLLSGPSPIRNIQLSATKSSESNSIDYLTDTQNIFPNFV---NNENNYQFS 101
Query: 489 EAVPAPV 509
A P+
Sbjct: 102 TAPLNPI 108
>SPBC3B8.04c |||membrane transporter|Schizosaccharomyces pombe|chr
2|||Manual
Length = 867
Score = 24.6 bits (51), Expect = 9.2
Identities = 13/31 (41%), Positives = 15/31 (48%)
Frame = -1
Query: 330 GRNICPKGPERTESMVPGSRSTRMARGTYLP 238
G NI PER + +S RMAR LP
Sbjct: 106 GNNINYPTPERLQKSSASRKSGRMARSQELP 136
>SPAC1D4.11c |lkh1|kic1|dual specificity protein kinase
Lkh1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 690
Score = 24.6 bits (51), Expect = 9.2
Identities = 12/28 (42%), Positives = 15/28 (53%)
Frame = +3
Query: 441 PNHVIVYKDSNSHTLSEAVPAPVWAHYL 524
P VIV +DS S L+ +P P H L
Sbjct: 23 PQEVIVIEDSASPRLTPNLPPPFSVHQL 50
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,970,267
Number of Sequences: 5004
Number of extensions: 37041
Number of successful extensions: 108
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 104
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 105
length of database: 2,362,478
effective HSP length: 68
effective length of database: 2,022,206
effective search space used: 216376042
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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