Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= S06A01NCLL0004_O04
(558 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces pomb... 77 2e-15
SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|ch... 75 9e-15
SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces... 55 6e-09
SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr 3|||Ma... 29 0.61
SPBP35G2.06c |nup131|Nup133a|nucleoporin Nup131|Schizosaccharomy... 27 2.5
SPBC215.01 ||SPBC3B9.20|GTPase activating protein|Schizosaccharo... 27 2.5
SPAC10F6.02c |prp22||ATP-dependent RNA helicase Prp22|Schizosacc... 25 5.7
SPAC17G6.12 |cul1|pcu1|cullin 1|Schizosaccharomyces pombe|chr 1|... 25 10.0
>SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 141
Score = 77.0 bits (181), Expect = 2e-15
Identities = 44/135 (32%), Positives = 75/135 (55%)
Frame = +3
Query: 96 AFSIYDFEGKGKIDAYNLGDLLRALNSNPTLATVXXXXXXXXXXXXXXXIEEFLPIYSQA 275
AFS++D G G+I ++GDLLRA NPTLA + +E+FL + ++
Sbjct: 11 AFSLFDRHGTGRIPKTSIGDLLRACGQNPTLAEITEIESTLPAEVD---MEQFLQVLNRP 67
Query: 276 KKDKDQGCYEDFLECLKLYDKNENGLMLGAELTHTLLALGEKLNDSEVAEITKDCMDPED 455
G E+F++ +++DK+ G++ EL + L +LGEKL++ E+ E+ K
Sbjct: 68 NGFDMPGDPEEFVKGFQVFDKDATGMIGVGELRYVLTSLGEKLSNEEMDELLKGV---PV 124
Query: 456 EDGMIPYAPFLKKVL 500
+DGM+ Y F++ +L
Sbjct: 125 KDGMVNYHDFVQMIL 139
>SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|chr
1|||Manual
Length = 150
Score = 74.5 bits (175), Expect = 9e-15
Identities = 46/147 (31%), Positives = 75/147 (51%), Gaps = 1/147 (0%)
Frame = +3
Query: 60 DLSKNDIERASFAFSIYDFEGKGKIDAYNLGDLLRALNSNPTLATVXXXXXXXXXXXXXX 239
+L+ I AFS++D + G I + LG ++R+L +PT A +
Sbjct: 5 NLTDEQIAEFREAFSLFDRDQDGNITSNELGVVMRSLGQSPTAAELQDMINEVDADGNGT 64
Query: 240 X-IEEFLPIYSQAKKDKDQGCYEDFLECLKLYDKNENGLMLGAELTHTLLALGEKLNDSE 416
EFL + ++ KD D E+ E K++DK+ NG + ELTH L +LGE+L+ E
Sbjct: 65 IDFTEFLTMMARKMKDTDNE--EEVREAFKVFDKDGNGYITVEELTHVLTSLGERLSQEE 122
Query: 417 VAEITKDCMDPEDEDGMIPYAPFLKKV 497
VA++ ++ D DG+I Y F + +
Sbjct: 123 VADMIREA--DTDGDGVINYEEFSRVI 147
>SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 143
Score = 55.2 bits (127), Expect = 6e-09
Identities = 35/145 (24%), Positives = 70/145 (48%)
Frame = +3
Query: 66 SKNDIERASFAFSIYDFEGKGKIDAYNLGDLLRALNSNPTLATVXXXXXXXXXXXXXXXI 245
SK + AF +YD + G I ++G +LR+L N T A +
Sbjct: 4 SKEQTDEMKEAFVLYDIDKDGLIPTSHVGSVLRSLGINVTDAELAKLSNELGDAIDEKKF 63
Query: 246 EEFLPIYSQAKKDKDQGCYEDFLECLKLYDKNENGLMLGAELTHTLLALGEKLNDSEVAE 425
F+ + K ++ E++++ +++DK+ +G + A+ + LGEKL+D+EV
Sbjct: 64 MSFV-----SNKLRETESEEEYIKAFRVFDKDNSGYIETAKFADYMKTLGEKLSDNEVQL 118
Query: 426 ITKDCMDPEDEDGMIPYAPFLKKVL 500
+ ++ DP + G Y F+++++
Sbjct: 119 MVQEA-DPTN-SGSFDYYDFVQRIM 141
>SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr
3|||Manual
Length = 176
Score = 28.7 bits (61), Expect = 0.61
Identities = 15/45 (33%), Positives = 24/45 (53%)
Frame = +3
Query: 303 EDFLECLKLYDKNENGLMLGAELTHTLLALGEKLNDSEVAEITKD 437
+D E KL+D +++ + EL + ALG SEV +I +D
Sbjct: 37 QDINEAFKLFDSDKDNAIDYHELRAAMRALGFNAEKSEVLKILRD 81
Score = 27.5 bits (58), Expect = 1.4
Identities = 12/39 (30%), Positives = 21/39 (53%)
Frame = +3
Query: 63 LSKNDIERASFAFSIYDFEGKGKIDAYNLGDLLRALNSN 179
+ ++ +E AF ++D + GKI NL + + LN N
Sbjct: 104 VERDPLEEIKRAFELFDDDETGKISLRNLRRVAKELNEN 142
>SPBP35G2.06c |nup131|Nup133a|nucleoporin Nup131|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 1142
Score = 26.6 bits (56), Expect = 2.5
Identities = 10/22 (45%), Positives = 14/22 (63%)
Frame = +3
Query: 390 LGEKLNDSEVAEITKDCMDPED 455
L EK+ D + I DC+DP+D
Sbjct: 777 LAEKVKDFQTMVILLDCLDPKD 798
>SPBC215.01 ||SPBC3B9.20|GTPase activating
protein|Schizosaccharomyces pombe|chr 2|||Manual
Length = 834
Score = 26.6 bits (56), Expect = 2.5
Identities = 12/29 (41%), Positives = 18/29 (62%), Gaps = 1/29 (3%)
Frame = +3
Query: 54 MSDLSKNDIER-ASFAFSIYDFEGKGKID 137
+++L D+ R SF F +YDF G G +D
Sbjct: 605 IAELKFRDVMRNISFIFELYDFNGDGFMD 633
>SPAC10F6.02c |prp22||ATP-dependent RNA helicase
Prp22|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1168
Score = 25.4 bits (53), Expect = 5.7
Identities = 10/30 (33%), Positives = 19/30 (63%)
Frame = +3
Query: 273 AKKDKDQGCYEDFLECLKLYDKNENGLMLG 362
AK+D +GCY+ +E +Y + +G++ G
Sbjct: 1062 AKRDSHEGCYKTIVENAPVY-MHPSGVLFG 1090
>SPAC17G6.12 |cul1|pcu1|cullin 1|Schizosaccharomyces pombe|chr
1|||Manual
Length = 767
Score = 24.6 bits (51), Expect = 10.0
Identities = 9/22 (40%), Positives = 12/22 (54%)
Frame = +3
Query: 288 DQGCYEDFLECLKLYDKNENGL 353
DQ C ED + +L + NGL
Sbjct: 305 DQNCSEDIIRMYRLMSRTPNGL 326
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,169,669
Number of Sequences: 5004
Number of extensions: 39657
Number of successful extensions: 115
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 108
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 110
length of database: 2,362,478
effective HSP length: 69
effective length of database: 2,017,202
effective search space used: 233995432
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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