Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= S06A01NCLL0004_I03
(471 letters)
Database: uniref50
1,657,284 sequences; 575,637,011 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
UniRef50_Q9UK41 Cluster: Vacuolar protein sorting-associated pro... 126 3e-28
UniRef50_Q8WZL1 Cluster: Vps28 protein; n=1; Yarrowia lipolytica... 75 1e-12
UniRef50_Q5KNG8 Cluster: Vps28 protein, putative; n=1; Filobasid... 73 2e-12
UniRef50_Q9NA26 Cluster: Vacuolar protein sorting-associated pro... 71 1e-11
UniRef50_Q9S9T7 Cluster: Vacuolar protein sorting-associated pro... 71 2e-11
UniRef50_Q4P529 Cluster: Putative uncharacterized protein; n=1; ... 69 7e-11
UniRef50_Q02767 Cluster: Vacuolar protein sorting-associated pro... 56 4e-07
UniRef50_A4RRM3 Cluster: Predicted protein; n=2; Ostreococcus|Re... 55 7e-07
UniRef50_A2WVR1 Cluster: Putative uncharacterized protein; n=3; ... 52 8e-06
UniRef50_Q86E08 Cluster: Clone ZZZ359 mRNA sequence; n=1; Schist... 46 6e-04
UniRef50_Q6BWP7 Cluster: Debaryomyces hansenii chromosome B of s... 46 6e-04
UniRef50_Q383K5 Cluster: Vacuolar sorting-associated protein-lik... 44 0.002
UniRef50_Q4Q0P5 Cluster: Vacuolar protein sorting-associated pro... 43 0.004
UniRef50_O13872 Cluster: Vacuolar protein sorting-associated pro... 39 0.048
UniRef50_A7AS71 Cluster: Vacuolar sorting protein 28, putative; ... 35 0.78
UniRef50_Q8Y9S8 Cluster: Lmo0445 protein; n=3; Listeria monocyto... 33 3.2
UniRef50_A2ZH03 Cluster: Putative uncharacterized protein; n=2; ... 33 3.2
UniRef50_Q3ABJ2 Cluster: Putative prophage LambdaCh01, scaffold ... 32 5.5
UniRef50_Q2TYZ1 Cluster: Predicted protein; n=2; Aspergillus|Rep... 31 9.7
UniRef50_Q1E2I4 Cluster: Putative uncharacterized protein; n=1; ... 31 9.7
>UniRef50_Q9UK41 Cluster: Vacuolar protein sorting-associated
protein 28 homolog; n=36; Eumetazoa|Rep: Vacuolar
protein sorting-associated protein 28 homolog - Homo
sapiens (Human)
Length = 221
Score = 126 bits (303), Expect = 3e-28
Identities = 57/77 (74%), Positives = 67/77 (87%)
Frame = +2
Query: 239 RPELYEEVKLYRNAREREKHDNMAELYAVVSTLQHLEKAYMRDCVRAQEYTAACSRLLVQ 418
+PELYEEVKLY+NAREREK+DNMAEL+AVV T+Q LEKAY++DCV EYTAACSRLLVQ
Sbjct: 17 KPELYEEVKLYKNAREREKYDNMAELFAVVKTMQALEKAYIKDCVSPSEYTAACSRLLVQ 76
Query: 419 YKVAFKQVQADEFPNIE 469
YK AF+QVQ E +I+
Sbjct: 77 YKAAFRQVQGSEISSID 93
>UniRef50_Q8WZL1 Cluster: Vps28 protein; n=1; Yarrowia
lipolytica|Rep: Vps28 protein - Yarrowia lipolytica
(Candida lipolytica)
Length = 246
Score = 74.5 bits (175), Expect = 1e-12
Identities = 30/63 (47%), Positives = 49/63 (77%)
Frame = +2
Query: 248 LYEEVKLYRNAREREKHDNMAELYAVVSTLQHLEKAYMRDCVRAQEYTAACSRLLVQYKV 427
L EEV+LY + +ERE+++N+AELY+++ +L +LEKA+++D V Q+Y++ C+RLL QY
Sbjct: 21 LNEEVRLYSSPKERERYENLAELYSIIVSLDYLEKAFLKDSVSQQDYSSICTRLLSQYNT 80
Query: 428 AFK 436
K
Sbjct: 81 LLK 83
>UniRef50_Q5KNG8 Cluster: Vps28 protein, putative; n=1;
Filobasidiella neoformans|Rep: Vps28 protein, putative -
Cryptococcus neoformans (Filobasidiella neoformans)
Length = 184
Score = 73.3 bits (172), Expect = 2e-12
Identities = 35/74 (47%), Positives = 51/74 (68%)
Frame = +2
Query: 248 LYEEVKLYRNAREREKHDNMAELYAVVSTLQHLEKAYMRDCVRAQEYTAACSRLLVQYKV 427
L EEV+L+ EREK +N+A LY+++ +L++LE+AY+RD V +EY AC +LL QYK
Sbjct: 4 LDEEVRLWTTNAEREKTENLATLYSIIVSLEYLERAYVRDSVSGKEYAPACLKLLAQYKS 63
Query: 428 AFKQVQADEFPNIE 469
K V AD+ +E
Sbjct: 64 LMKLV-ADDIGGVE 76
>UniRef50_Q9NA26 Cluster: Vacuolar protein sorting-associated
protein 28 homolog; n=2; Caenorhabditis|Rep: Vacuolar
protein sorting-associated protein 28 homolog -
Caenorhabditis elegans
Length = 210
Score = 70.9 bits (166), Expect = 1e-11
Identities = 32/72 (44%), Positives = 47/72 (65%)
Frame = +2
Query: 227 MQDTRPELYEEVKLYRNAREREKHDNMAELYAVVSTLQHLEKAYMRDCVRAQEYTAACSR 406
M L EV+L+ N ERE+ +N++EL+AV++ L+HLEK + RD V A EY + C +
Sbjct: 1 MSSQNANLMREVRLFENHSEREQMENLSELFAVLNALEHLEKMFSRDHVSADEYKSECFK 60
Query: 407 LLVQYKVAFKQV 442
L+ QYKV + V
Sbjct: 61 LIDQYKVTMRLV 72
>UniRef50_Q9S9T7 Cluster: Vacuolar protein sorting-associated
protein 28 homolog 1; n=4; Eukaryota|Rep: Vacuolar
protein sorting-associated protein 28 homolog 1 -
Arabidopsis thaliana (Mouse-ear cress)
Length = 210
Score = 70.5 bits (165), Expect = 2e-11
Identities = 31/71 (43%), Positives = 47/71 (66%)
Frame = +2
Query: 257 EVKLYRNAREREKHDNMAELYAVVSTLQHLEKAYMRDCVRAQEYTAACSRLLVQYKVAFK 436
EVKL+ + RERE ++N AEL+A++ + LEKAY+RD + EY + C +L+V +K
Sbjct: 3 EVKLWNDKREREMYENFAELFAIIKATEKLEKAYIRDLINPSEYESECQKLIVHFKTLSA 62
Query: 437 QVQADEFPNIE 469
++ D PNIE
Sbjct: 63 TLK-DTVPNIE 72
>UniRef50_Q4P529 Cluster: Putative uncharacterized protein; n=1;
Ustilago maydis|Rep: Putative uncharacterized protein -
Ustilago maydis (Smut fungus)
Length = 170
Score = 68.5 bits (160), Expect = 7e-11
Identities = 28/68 (41%), Positives = 45/68 (66%)
Frame = +2
Query: 251 YEEVKLYRNAREREKHDNMAELYAVVSTLQHLEKAYMRDCVRAQEYTAACSRLLVQYKVA 430
YEE +LY N +RE+++NMA L++++ L +LE+AY+R+ + +EY C+RLL Q K
Sbjct: 12 YEEARLYSNNHDRERYENMATLFSLIVALDYLERAYVRESISEKEYAPTCTRLLAQCKTM 71
Query: 431 FKQVQADE 454
K + E
Sbjct: 72 LKLIVDQE 79
>UniRef50_Q02767 Cluster: Vacuolar protein sorting-associated
protein 28; n=10; Saccharomycetales|Rep: Vacuolar
protein sorting-associated protein 28 - Saccharomyces
cerevisiae (Baker's yeast)
Length = 242
Score = 56.0 bits (129), Expect = 4e-07
Identities = 26/78 (33%), Positives = 47/78 (60%), Gaps = 3/78 (3%)
Frame = +2
Query: 230 QDTRPELYEEVKLYRNA---REREKHDNMAELYAVVSTLQHLEKAYMRDCVRAQEYTAAC 400
QD ++EV L+ N+ +++E + ++E+Y++V TL H+EKAY++D + +YT
Sbjct: 12 QDISQLFHDEVPLFDNSITSKDKEVIETLSEIYSIVITLDHVEKAYLKDSIDDTQYTNTV 71
Query: 401 SRLLVQYKVAFKQVQADE 454
+LL Q+KV +E
Sbjct: 72 DKLLKQFKVYLNSQNKEE 89
>UniRef50_A4RRM3 Cluster: Predicted protein; n=2; Ostreococcus|Rep:
Predicted protein - Ostreococcus lucimarinus CCE9901
Length = 218
Score = 55.2 bits (127), Expect = 7e-07
Identities = 27/72 (37%), Positives = 44/72 (61%)
Frame = +2
Query: 254 EEVKLYRNAREREKHDNMAELYAVVSTLQHLEKAYMRDCVRAQEYTAACSRLLVQYKVAF 433
E V+L+R ER+++D+ A LYA+ L+ LE+AY+R V A+ Y AC L ++K
Sbjct: 11 ECVRLWRTTDERKRYDDFATLYALARALEKLERAYVRSSVDAKAYERACVDLTSKFK-TL 69
Query: 434 KQVQADEFPNIE 469
+ V D P+++
Sbjct: 70 RSVLRDSVPDLD 81
>UniRef50_A2WVR1 Cluster: Putative uncharacterized protein; n=3;
Oryza sativa|Rep: Putative uncharacterized protein -
Oryza sativa subsp. indica (Rice)
Length = 253
Score = 51.6 bits (118), Expect = 8e-06
Identities = 21/47 (44%), Positives = 34/47 (72%)
Frame = +2
Query: 281 REREKHDNMAELYAVVSTLQHLEKAYMRDCVRAQEYTAACSRLLVQY 421
RERE +++A+LYA++ + LE+AY+RD V A +Y A C +L+ Q+
Sbjct: 50 RERELLESLADLYAIIKATEKLERAYVRDLVSAADYEAECLKLISQF 96
>UniRef50_Q86E08 Cluster: Clone ZZZ359 mRNA sequence; n=1;
Schistosoma japonicum|Rep: Clone ZZZ359 mRNA sequence -
Schistosoma japonicum (Blood fluke)
Length = 214
Score = 45.6 bits (103), Expect = 6e-04
Identities = 20/30 (66%), Positives = 23/30 (76%)
Frame = +2
Query: 380 QEYTAACSRLLVQYKVAFKQVQADEFPNIE 469
Q AACS+LLVQY+ AFKQVQ DEF +E
Sbjct: 36 QSIHAACSKLLVQYEAAFKQVQGDEFSTVE 65
>UniRef50_Q6BWP7 Cluster: Debaryomyces hansenii chromosome B of
strain CBS767 of Debaryomyces hansenii; n=3;
Saccharomycetaceae|Rep: Debaryomyces hansenii chromosome
B of strain CBS767 of Debaryomyces hansenii -
Debaryomyces hansenii (Yeast) (Torulaspora hansenii)
Length = 280
Score = 45.6 bits (103), Expect = 6e-04
Identities = 17/54 (31%), Positives = 39/54 (72%), Gaps = 1/54 (1%)
Frame = +2
Query: 296 HDNMAELYAVVSTLQHLEKAYMRDCVRAQE-YTAACSRLLVQYKVAFKQVQADE 454
+D++AE+Y++V+ ++ +EK++++D + ++ YT+ RL+ QY++ K Q D+
Sbjct: 47 YDSLAEIYSIVTVMEMIEKSFLKDYITDKDKYTSTTLRLINQYQIIIKGFQEDQ 100
>UniRef50_Q383K5 Cluster: Vacuolar sorting-associated protein-like,
putative; n=2; Trypanosoma|Rep: Vacuolar
sorting-associated protein-like, putative - Trypanosoma
brucei
Length = 218
Score = 43.6 bits (98), Expect = 0.002
Identities = 19/68 (27%), Positives = 40/68 (58%)
Frame = +2
Query: 257 EVKLYRNAREREKHDNMAELYAVVSTLQHLEKAYMRDCVRAQEYTAACSRLLVQYKVAFK 436
EV + ER+ + +A+L+A++ ++ +EKA +RD + ++Y++ +RLL +YK
Sbjct: 2 EVAFTISPGERQHVEYLADLFAIILAIEKVEKATLRDIITQEQYSSTITRLLDKYKSTVT 61
Query: 437 QVQADEFP 460
++ P
Sbjct: 62 YLEQSRNP 69
>UniRef50_Q4Q0P5 Cluster: Vacuolar protein sorting-associated
protein-like protein; n=3; Leishmania|Rep: Vacuolar
protein sorting-associated protein-like protein -
Leishmania major
Length = 209
Score = 42.7 bits (96), Expect = 0.004
Identities = 19/59 (32%), Positives = 33/59 (55%)
Frame = +2
Query: 284 EREKHDNMAELYAVVSTLQHLEKAYMRDCVRAQEYTAACSRLLVQYKVAFKQVQADEFP 460
+R+ D +A+L+AV+ ++ +EKA RD + +Y + RLL +YK +Q P
Sbjct: 10 DRQHIDYLADLFAVIIAIEQIEKANRRDLINPNQYDSTVRRLLEKYKNTVAHLQGARNP 68
>UniRef50_O13872 Cluster: Vacuolar protein sorting-associated
protein 28 homolog; n=1; Schizosaccharomyces pombe|Rep:
Vacuolar protein sorting-associated protein 28 homolog -
Schizosaccharomyces pombe (Fission yeast)
Length = 248
Score = 39.1 bits (87), Expect = 0.048
Identities = 13/53 (24%), Positives = 33/53 (62%)
Frame = +2
Query: 275 NAREREKHDNMAELYAVVSTLQHLEKAYMRDCVRAQEYTAACSRLLVQYKVAF 433
+ + ++ ++++ LY+++ L+ LEKA+ +D V ++ + C L+ Q++ F
Sbjct: 21 HTKNQQVREDLSILYSILVALEQLEKAFTKDAVSTSDFNSTCELLIQQWESCF 73
>UniRef50_A7AS71 Cluster: Vacuolar sorting protein 28, putative;
n=1; Babesia bovis|Rep: Vacuolar sorting protein 28,
putative - Babesia bovis
Length = 222
Score = 35.1 bits (77), Expect = 0.78
Identities = 13/38 (34%), Positives = 24/38 (63%)
Frame = +2
Query: 299 DNMAELYAVVSTLQHLEKAYMRDCVRAQEYTAACSRLL 412
D +A +Y+++ L+HLE +++ V +EY C+ LL
Sbjct: 17 DRLANVYSLLQALEHLEDSFISGYVTEKEYAEECNELL 54
>UniRef50_Q8Y9S8 Cluster: Lmo0445 protein; n=3; Listeria
monocytogenes|Rep: Lmo0445 protein - Listeria
monocytogenes
Length = 495
Score = 33.1 bits (72), Expect = 3.2
Identities = 15/37 (40%), Positives = 25/37 (67%)
Frame = +2
Query: 212 YFYTNMQDTRPELYEEVKLYRNAREREKHDNMAELYA 322
YF+ + +TRP+LYE ++ R A E K +N +++YA
Sbjct: 264 YFFYLLMETRPKLYENKEVARRALEPHKKNN-SDVYA 299
>UniRef50_A2ZH03 Cluster: Putative uncharacterized protein; n=2;
Oryza sativa (indica cultivar-group)|Rep: Putative
uncharacterized protein - Oryza sativa subsp. indica
(Rice)
Length = 429
Score = 33.1 bits (72), Expect = 3.2
Identities = 22/63 (34%), Positives = 36/63 (57%), Gaps = 2/63 (3%)
Frame = -3
Query: 466 NIWKLISLHLFECNFILHKEATTSCSV--FLSPDTISHVSFLKVL*CADHSIELSHIVML 293
++ LI+L L +C L + + + CS+ P+ + H+ +LKV+ C SIE SH + L
Sbjct: 188 DLGNLINLFLAKCP-ALERLSLSHCSMTDLNIPNPLCHLQYLKVVNCCVQSIE-SHAMSL 245
Query: 292 FTF 284
TF
Sbjct: 246 TTF 248
>UniRef50_Q3ABJ2 Cluster: Putative prophage LambdaCh01, scaffold
protein; n=1; Carboxydothermus hydrogenoformans
Z-2901|Rep: Putative prophage LambdaCh01, scaffold
protein - Carboxydothermus hydrogenoformans (strain
Z-2901 / DSM 6008)
Length = 196
Score = 32.3 bits (70), Expect = 5.5
Identities = 23/70 (32%), Positives = 34/70 (48%)
Frame = +2
Query: 236 TRPELYEEVKLYRNAREREKHDNMAELYAVVSTLQHLEKAYMRDCVRAQEYTAACSRLLV 415
T+ EL E + R REREK + AEL LQ L++A M + + Q R+L
Sbjct: 32 TQEEL-ERIIAERLKREREKFKDYAELKKAAEELQKLKEAQMTEQEKLQAKLQEYERILQ 90
Query: 416 QYKVAFKQVQ 445
+ + K+ Q
Sbjct: 91 EKERETKEAQ 100
>UniRef50_Q2TYZ1 Cluster: Predicted protein; n=2; Aspergillus|Rep:
Predicted protein - Aspergillus oryzae
Length = 1374
Score = 31.5 bits (68), Expect = 9.7
Identities = 18/81 (22%), Positives = 45/81 (55%), Gaps = 4/81 (4%)
Frame = +2
Query: 224 NMQDTRPELYEEVKLYRNARE---REKHDNMAELYAVVSTLQHLEKAYMRDCV-RAQEYT 391
N+ + R +L E+++ + E R KHD E+ + ++++ ++A+ ++C R QE
Sbjct: 791 NLDEKRKDLEEKLEESERSNEELTRNKHDLEEEVRTLNTSMKEAQEAHEKECERREQEKV 850
Query: 392 AACSRLLVQYKVAFKQVQADE 454
A + + +F++++A++
Sbjct: 851 DALATQKREMTESFEEIKAED 871
>UniRef50_Q1E2I4 Cluster: Putative uncharacterized protein; n=1;
Coccidioides immitis|Rep: Putative uncharacterized
protein - Coccidioides immitis
Length = 662
Score = 31.5 bits (68), Expect = 9.7
Identities = 15/34 (44%), Positives = 20/34 (58%)
Frame = +2
Query: 248 LYEEVKLYRNAREREKHDNMAELYAVVSTLQHLE 349
L EEVKL R+A+ER K + YA+ + LE
Sbjct: 178 LKEEVKLERDAKERAKQEAFERAYALAGLTEELE 211
Database: uniref50
Posted date: Oct 5, 2007 11:19 AM
Number of letters in database: 575,637,011
Number of sequences in database: 1,657,284
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 420,982,837
Number of Sequences: 1657284
Number of extensions: 7779648
Number of successful extensions: 21580
Number of sequences better than 10.0: 20
Number of HSP's better than 10.0 without gapping: 20822
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 21575
length of database: 575,637,011
effective HSP length: 94
effective length of database: 419,852,315
effective search space used: 26030843530
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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