Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= S06A01NCLL0004_H08
(546 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC794.12c |mae2||malic enzyme|Schizosaccharomyces pombe|chr 3|... 73 4e-14
SPAC750.08c |||NAD-dependent malic enzyme|Schizosaccharomyces po... 68 1e-12
SPAC22E12.16c |pik1||phosphatidylinositol kinase Pik1|Schizosacc... 29 0.59
SPBC21.06c |cdc7|pld1, its10|serine/threonine protein kinase Cdc... 27 1.8
SPBC19C7.09c |uve1|uvde|endonuclease Uve1 |Schizosaccharomyces p... 25 5.5
SPAC13G6.03 |gpi7||GPI anchor biosynthesis protein Gpi7 |Schizos... 25 7.3
SPBC12C2.13c |fnx1|SPBC21D10.04c|MFS multidrug efflux transporte... 25 7.3
SPBC776.09 |ste13||ATP-dependent RNA helicase Ste13|Schizosaccha... 25 9.6
SPBC30B4.04c |sol1||SWI/SNF complex subunit Sol1|Schizosaccharom... 25 9.6
SPBC36B7.03 |sec63||ER protein translocation subcomplex subunit ... 25 9.6
SPBC16E9.02c |||CUE domain protein Cue5 |Schizosaccharomyces pom... 25 9.6
>SPCC794.12c |mae2||malic enzyme|Schizosaccharomyces pombe|chr
3|||Manual
Length = 565
Score = 72.5 bits (170), Expect = 4e-14
Identities = 42/145 (28%), Positives = 75/145 (51%), Gaps = 6/145 (4%)
Frame = +3
Query: 69 IWMVDSRGLIVKNRPEGGLNEHKEKFAKDHPPVRT------LAEVVDLITPSVLIGAAAI 230
++M+D GL+++ + + K KD L + L+ P++L+G +
Sbjct: 334 LFMIDRCGLLLERHAKIATDGQKPFLKKDSDFKEVPSGDINLESAIALVKPTILLGCSGQ 393
Query: 231 GGAFTPDILRKMGACNDRPVIFALSNPTSKAECTAEEAYSNTDDRAIFASGSPFPEYRTK 410
G FT +R+M +RP+IF +SNPT+ E ++ +D +A+ A+GSP P +
Sbjct: 394 PGKFTEKAIREMSKHVERPIIFPISNPTTLMEAKPDQIDKWSDGKALIATGSPLPPL-NR 452
Query: 411 DGRVLRTGQGNNSYIFPGLALGIIC 485
+G+ Q NN+ ++P ALG+ C
Sbjct: 453 NGKKYVISQCNNALLYP--ALGVAC 475
>SPAC750.08c |||NAD-dependent malic enzyme|Schizosaccharomyces
pombe|chr 1||Partial|Manual
Length = 228
Score = 67.7 bits (158), Expect = 1e-12
Identities = 36/104 (34%), Positives = 58/104 (55%)
Frame = +3
Query: 174 LAEVVDLITPSVLIGAAAIGGAFTPDILRKMGACNDRPVIFALSNPTSKAECTAEEAYSN 353
L + LI P+VL+G + G FT +R+M P+IF +SNPT+ E +
Sbjct: 38 LETAISLIKPTVLLGCSGQPGKFTEKAIREMSKHVKHPIIFPISNPTTLMEAKPVQIDEW 97
Query: 354 TDDRAIFASGSPFPEYRTKDGRVLRTGQGNNSYIFPGLALGIIC 485
++ +A+ A+GSP P T++G+ Q NN+ ++P ALG+ C
Sbjct: 98 SNGKALMATGSPLPPL-TRNGKEYVISQCNNALLYP--ALGVAC 138
>SPAC22E12.16c |pik1||phosphatidylinositol kinase
Pik1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 851
Score = 28.7 bits (61), Expect = 0.59
Identities = 8/35 (22%), Positives = 23/35 (65%)
Frame = +3
Query: 165 VRTLAEVVDLITPSVLIGAAAIGGAFTPDILRKMG 269
+++ ++ + +TP++++ +GG P++L+K G
Sbjct: 135 IQSQQKISENVTPALILSGIMLGGVCVPELLKKAG 169
>SPBC21.06c |cdc7|pld1, its10|serine/threonine protein kinase
Cdc7|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1062
Score = 27.1 bits (57), Expect = 1.8
Identities = 11/21 (52%), Positives = 14/21 (66%), Gaps = 1/21 (4%)
Frame = +3
Query: 111 PEGGLNEHKEKFAKDH-PPVR 170
P+G +NEHK +K H PP R
Sbjct: 1033 PQGPINEHKSPISKPHMPPPR 1053
>SPBC19C7.09c |uve1|uvde|endonuclease Uve1 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 599
Score = 25.4 bits (53), Expect = 5.5
Identities = 11/39 (28%), Positives = 15/39 (38%)
Frame = +2
Query: 200 PICSHRRGGYRRRVHSGHTAQDGRLQRPTRHIRALEPDQ 316
P SH + GY H + G+L H + P Q
Sbjct: 319 PFASHAKYGYTLEFAQSHLEEVGKLANKYNHRLTMHPGQ 357
>SPAC13G6.03 |gpi7||GPI anchor biosynthesis protein Gpi7
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 758
Score = 25.0 bits (52), Expect = 7.3
Identities = 12/35 (34%), Positives = 17/35 (48%)
Frame = +3
Query: 279 DRPVIFALSNPTSKAECTAEEAYSNTDDRAIFASG 383
++ F+L N S A +AY+ D IFA G
Sbjct: 637 EQVAFFSLGNSNSLATVDLSQAYTGLDSYNIFAVG 671
>SPBC12C2.13c |fnx1|SPBC21D10.04c|MFS multidrug efflux
transporter|Schizosaccharomyces pombe|chr 2|||Manual
Length = 531
Score = 25.0 bits (52), Expect = 7.3
Identities = 9/20 (45%), Positives = 12/20 (60%)
Frame = -2
Query: 497 DDPGADDAERESREYVGVVP 438
DDP DD R RE + ++P
Sbjct: 461 DDPDKDDLIRRLRESISIIP 480
>SPBC776.09 |ste13||ATP-dependent RNA helicase
Ste13|Schizosaccharomyces pombe|chr 2|||Manual
Length = 485
Score = 24.6 bits (51), Expect = 9.6
Identities = 15/37 (40%), Positives = 21/37 (56%)
Frame = +2
Query: 260 QDGRLQRPTRHIRALEPDQQGGVHRRGGLQQHRRPRY 370
Q+G RP + R P +GG +R G Q +R+PRY
Sbjct: 445 QEGYHNRPNNN-RGGHP--RGGGNRGGYRQSNRQPRY 478
>SPBC30B4.04c |sol1||SWI/SNF complex subunit
Sol1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 865
Score = 24.6 bits (51), Expect = 9.6
Identities = 12/37 (32%), Positives = 18/37 (48%), Gaps = 1/37 (2%)
Frame = -3
Query: 544 QLEPRQLGA*SHPDISTIPAQM-MPSASPGNM*ELFP 437
Q+ P Q+G +HP + P P+ GN +L P
Sbjct: 149 QIPPNQIGINAHPAVRQTPQPAPSPNTPSGNANQLTP 185
>SPBC36B7.03 |sec63||ER protein translocation subcomplex subunit
Sec63 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 611
Score = 24.6 bits (51), Expect = 9.6
Identities = 15/37 (40%), Positives = 19/37 (51%)
Frame = +3
Query: 387 PFPEYRTKDGRVLRTGQGNNSYIFPGLALGIICAGIV 497
PF +YR KD +V R YIF L LG + G +
Sbjct: 49 PFQKYRPKDLKVQRKSIFRLRYIF--LILGWLAIGFL 83
>SPBC16E9.02c |||CUE domain protein Cue5 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 569
Score = 24.6 bits (51), Expect = 9.6
Identities = 11/20 (55%), Positives = 13/20 (65%)
Frame = +3
Query: 318 KAECTAEEAYSNTDDRAIFA 377
KA T +EA+ NTDD I A
Sbjct: 20 KARATLKEAFPNTDDAIIRA 39
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,651,197
Number of Sequences: 5004
Number of extensions: 29429
Number of successful extensions: 95
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 91
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 95
length of database: 2,362,478
effective HSP length: 69
effective length of database: 2,017,202
effective search space used: 225926624
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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