Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= S06A01NCLL0004_F02
(587 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 221 5e-59
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 135 4e-33
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 129 3e-31
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 103 1e-23
SPCP1E11.06 |apl4||AP-1 adaptor complex gamma subunit Apl4 |Schi... 27 2.7
SPBC1D7.05 |byr2|ste8, SPBC2F12.01|MAP kinase kinase kinase Byr2... 26 4.7
SPAC644.07 |||Rieske ISP assembly protein|Schizosaccharomyces po... 25 8.2
SPCC777.15 |||tRNA dihydrouridine synthase Dus4 |Schizosaccharom... 25 8.2
>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 448
Score = 221 bits (541), Expect = 5e-59
Identities = 102/139 (73%), Positives = 119/139 (85%)
Frame = +2
Query: 11 DRIMNTYSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLSTP 190
DR+M T+SV P+PK SDTVVEPYNATLS+HQLVEN+DET+CIDNEAL I TLK+ +P
Sbjct: 161 DRMMATFSVAPAPKSSDTVVEPYNATLSMHQLVENSDETFCIDNEALSSIFANTLKIKSP 220
Query: 191 TYGDLNHLVSLTMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSRQ 370
+Y DLNHLVS M+GVTT RFPG+LN+DLRKLAVNMVPFPRLHFFM GFAPL + GS
Sbjct: 221 SYDDLNHLVSAVMAGVTTSFRFPGELNSDLRKLAVNMVPFPRLHFFMVGFAPLAAIGSSS 280
Query: 371 YRALTVPELTQQMFDAKNM 427
++A++VPELTQQMFDA NM
Sbjct: 281 FQAVSVPELTQQMFDANNM 299
Score = 70.5 bits (165), Expect = 2e-13
Identities = 29/41 (70%), Positives = 39/41 (95%)
Frame = +1
Query: 460 RYLTVAAIFRGRMSMKDVDEQMLNIQKQNSSYFVEWIPNNV 582
RYLTVAA+FRG++SMK+VDEQ+ ++Q +NS+YFVEWIP+NV
Sbjct: 309 RYLTVAALFRGKVSMKEVDEQIRSVQTKNSAYFVEWIPDNV 349
>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 455
Score = 135 bits (327), Expect = 4e-33
Identities = 60/139 (43%), Positives = 91/139 (65%)
Frame = +2
Query: 29 YSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLSTPTYGDLN 208
+SV P+P+VS +VVEPYN+ L+ H ++N+D T+ +DNEA YDIC R L + PTY +LN
Sbjct: 173 FSVYPAPQVSTSVVEPYNSVLTTHATLDNSDCTFMVDNEACYDICRRNLDIERPTYENLN 232
Query: 209 HLVSLTMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSRQYRALTV 388
L++ +S +T LRF G LN DL + N+VP+PR+HF + ++P+ S + + +V
Sbjct: 233 RLIAQVVSSITASLRFAGSLNVDLNEFQTNLVPYPRIHFPLVTYSPIVSAAKAFHESNSV 292
Query: 389 PELTQQMFDAKNMHRRPCD 445
E+T Q F+ N + CD
Sbjct: 293 QEITNQCFEPYNQMVK-CD 310
Score = 35.5 bits (78), Expect = 0.006
Identities = 15/47 (31%), Positives = 26/47 (55%)
Frame = +1
Query: 445 PRVTSRYLTVAAIFRGRMSMKDVDEQMLNIQKQNSSYFVEWIPNNVK 585
PR T RY+ ++RG + +DV + +I+ + + FV+W P K
Sbjct: 311 PR-TGRYMATCLLYRGDVIPRDVQAAVTSIKSRRTIQFVDWCPTGFK 356
>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 449
Score = 129 bits (312), Expect = 3e-31
Identities = 58/139 (41%), Positives = 89/139 (64%)
Frame = +2
Query: 29 YSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLSTPTYGDLN 208
+SV P+P+VS +VVEPYN+ L+ H ++ D T+ +DNE+ YDIC R L + P+Y +LN
Sbjct: 169 FSVYPAPQVSTSVVEPYNSVLTTHATLDLADCTFMVDNESCYDICRRNLDIERPSYENLN 228
Query: 209 HLVSLTMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSRQYRALTV 388
L++ +S +T LRF G LN DL + N+VP+PR+HF + +AP+ S + + +V
Sbjct: 229 RLIAQVVSSITASLRFEGSLNVDLAEFQTNLVPYPRIHFPLVTYAPIVSAAKAFHESNSV 288
Query: 389 PELTQQMFDAKNMHRRPCD 445
E+T Q F+ N + CD
Sbjct: 289 QEITNQCFEPYNQMVK-CD 306
Score = 32.7 bits (71), Expect = 0.041
Identities = 14/47 (29%), Positives = 24/47 (51%)
Frame = +1
Query: 445 PRVTSRYLTVAAIFRGRMSMKDVDEQMLNIQKQNSSYFVEWIPNNVK 585
PR RY+ ++RG + +DV + I+ + + FV+W P K
Sbjct: 307 PRA-GRYMATCLLYRGDVIPRDVQAAVTTIKAKRTIQFVDWCPTGFK 352
>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 446
Score = 103 bits (248), Expect = 1e-23
Identities = 54/141 (38%), Positives = 81/141 (57%), Gaps = 4/141 (2%)
Frame = +2
Query: 14 RIMNTYSVVP-SPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLSTP 190
+I+ TYSV P S VSD VV+PYN+ L++ +L N D +DN AL I L P
Sbjct: 164 KIIQTYSVFPNSQSVSDVVVQPYNSLLALKRLTLNADSVVVLDNAALAHIAADRLHTQNP 223
Query: 191 TYGDLNHLVSLTMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSRQ 370
T+ N LVS MS TT LR+PG +N DL + +++P PR HF + + P T++ +
Sbjct: 224 TFHQQNQLVSTVMSASTTTLRYPGYMNNDLVSIIASLIPSPRCHFLLTSYTPFTNQQVEE 283
Query: 371 YRAL---TVPELTQQMFDAKN 424
+A+ TV ++ +++ KN
Sbjct: 284 AKAIRKTTVLDVMRRLLLPKN 304
>SPCP1E11.06 |apl4||AP-1 adaptor complex gamma subunit Apl4
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 865
Score = 26.6 bits (56), Expect = 2.7
Identities = 22/68 (32%), Positives = 32/68 (47%)
Frame = +2
Query: 101 QLVENTDETYCIDNEALYDICFRTLKLSTPTYGDLNHLVSLTMSGVTTCLRFPGQLNADL 280
Q+ NTD + N LY RT+ DLN SL + GV +F G + +
Sbjct: 301 QVCTNTDSSRNAGNAILYQ-AVRTIL-------DLNSDSSLRVLGVNILAKFLGNRDNNT 352
Query: 281 RKLAVNMV 304
R +A+NM+
Sbjct: 353 RYVALNML 360
>SPBC1D7.05 |byr2|ste8, SPBC2F12.01|MAP kinase kinase kinase
Byr2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 659
Score = 25.8 bits (54), Expect = 4.7
Identities = 15/49 (30%), Positives = 23/49 (46%), Gaps = 4/49 (8%)
Frame = -3
Query: 540 LLDVKHLLIDVLH----RHTTAEDGCDGEVAARDAXSHGRRCMFLASNI 406
L+ VK +++D + RH D GE+A SH +L SN+
Sbjct: 419 LMAVKQVILDSVSESKDRHAKLLDALAGEIALLQELSHEHIVQYLGSNL 467
>SPAC644.07 |||Rieske ISP assembly protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 449
Score = 25.0 bits (52), Expect = 8.2
Identities = 14/35 (40%), Positives = 16/35 (45%)
Frame = +2
Query: 116 TDETYCIDNEALYDICFRTLKLSTPTYGDLNHLVS 220
T Y + E YDIC L T LNHL+S
Sbjct: 256 TSFLYALAGELDYDICVLNLAEKGLTDDRLNHLLS 290
>SPCC777.15 |||tRNA dihydrouridine synthase Dus4
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 326
Score = 25.0 bits (52), Expect = 8.2
Identities = 10/18 (55%), Positives = 12/18 (66%)
Frame = +1
Query: 193 LWRSQSLSLPHHVWRHHL 246
LW S S SL H++ HHL
Sbjct: 257 LWYSTSYSLNFHLFYHHL 274
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,406,127
Number of Sequences: 5004
Number of extensions: 46753
Number of successful extensions: 188
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 179
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 188
length of database: 2,362,478
effective HSP length: 69
effective length of database: 2,017,202
effective search space used: 254167452
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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