Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= S06A01NCLL0004_E22
(306 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 135 2e-33
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 80 6e-17
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 70 7e-14
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 64 4e-12
SPAC21E11.08 |lcb2|SPAC2C4.02|serine palmitoyltransferase |Schiz... 29 0.15
SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||... 28 0.36
SPBC19G7.16 |iws1||transcription elongation factor complex subun... 27 0.62
SPBC1685.05 |||serine protease |Schizosaccharomyces pombe|chr 2|... 26 1.1
SPAC1805.16c |||purine nucleoside phosphorylase |Schizosaccharom... 26 1.1
SPAC11E3.05 |||ubiquitin-protein ligase E3|Schizosaccharomyces p... 26 1.4
SPCC74.02c |||mRNA cleavage and polyadenylation specificity fact... 25 3.3
SPAC30C2.07 |||sequence orphan|Schizosaccharomyces pombe|chr 1||... 23 7.7
SPCC1450.10c |||iron hydrogenase large subunit |Schizosaccharomy... 23 7.7
SPAPB1E7.04c |||chitinase |Schizosaccharomyces pombe|chr 1|||Manual 23 7.7
>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 448
Score = 135 bits (326), Expect = 2e-33
Identities = 57/75 (76%), Positives = 66/75 (88%)
Frame = +2
Query: 80 MREIVHIQAGQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERINVYYNEASGGKYV 259
MREIVHIQAGQCGNQ+GA FW I+DEHG+D G YHG S+ Q ER+NVY+NEA+GGKYV
Sbjct: 1 MREIVHIQAGQCGNQVGAAFWSTIADEHGLDSAGIYHGTSEAQHERLNVYFNEAAGGKYV 60
Query: 260 PRAILVDLEPGTMDS 304
PRA+LVDLEPGTMD+
Sbjct: 61 PRAVLVDLEPGTMDA 75
>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 446
Score = 80.2 bits (189), Expect = 6e-17
Identities = 33/74 (44%), Positives = 53/74 (71%)
Frame = +2
Query: 83 REIVHIQAGQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERINVYYNEASGGKYVP 262
REI+ +QAGQCGNQIG++FW+ + EHGI P G + ++R +V++ ++ +Y+P
Sbjct: 3 REIITLQAGQCGNQIGSQFWQQLCLEHGIGPDGTLESFATEGVDRKDVFFYQSDDTRYIP 62
Query: 263 RAILVDLEPGTMDS 304
RAIL+DLEP +++
Sbjct: 63 RAILIDLEPRVVNN 76
>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 449
Score = 70.1 bits (164), Expect = 7e-14
Identities = 34/76 (44%), Positives = 45/76 (59%), Gaps = 2/76 (2%)
Frame = +2
Query: 80 MREIVHIQAGQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLE--RINVYYNEASGGK 253
MREI+ I GQ G QIG WE+ EHGI P G + ++ Q + +++E GK
Sbjct: 1 MREIISIHVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGK 60
Query: 254 YVPRAILVDLEPGTMD 301
YVPR+I VDLEP +D
Sbjct: 61 YVPRSIYVDLEPNVID 76
>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 455
Score = 64.1 bits (149), Expect = 4e-12
Identities = 31/80 (38%), Positives = 44/80 (55%), Gaps = 6/80 (7%)
Frame = +2
Query: 80 MREIVHIQAGQCGNQIGAKFWEIISDEHGIDPTG------AYHGDSDLQLERINVYYNEA 241
MRE++ + GQ G QIG WE+ EHGI P G H ++ + +++E
Sbjct: 1 MREVISVHVGQAGVQIGNACWELYCLEHGIGPDGFPTENSEVHKNNSYLNDGFGTFFSET 60
Query: 242 SGGKYVPRAILVDLEPGTMD 301
GK+VPR+I VDLEP +D
Sbjct: 61 GQGKFVPRSIYVDLEPNVID 80
>SPAC21E11.08 |lcb2|SPAC2C4.02|serine palmitoyltransferase
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 603
Score = 29.1 bits (62), Expect = 0.15
Identities = 14/51 (27%), Positives = 27/51 (52%)
Frame = -3
Query: 166 AVLIGDDLPELSSNLVSALSGLDVHDFPHFDISELNKLLNTNIFCSARTTH 14
+++I D+L S + LSG ++ + H D+++L ++L I TH
Sbjct: 273 SLIISDELNHTSIRFGARLSGANIRVYKHNDMTDLERVLREVISQGQPRTH 323
>SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr
2|||Manual
Length = 534
Score = 27.9 bits (59), Expect = 0.36
Identities = 16/49 (32%), Positives = 27/49 (55%)
Frame = +3
Query: 90 SCTSRPDNAETRLELSSGRSSPMSTASTPPVLTTATQTCSWSASMFITM 236
S +SR + + LSS S P+ TAS+ +LT+++ T S +T+
Sbjct: 427 STSSRSSSHSSSHSLSSLSSKPILTASSSSLLTSSSHTYERSTVYVVTV 475
>SPBC19G7.16 |iws1||transcription elongation factor complex subunit
Iws1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 428
Score = 27.1 bits (57), Expect = 0.62
Identities = 10/34 (29%), Positives = 22/34 (64%)
Frame = +2
Query: 119 NQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERI 220
N++G E+++++ +DPT A + DLQ++ +
Sbjct: 133 NELGENEEEVLTEQKQLDPTLAAKKELDLQMDAV 166
>SPBC1685.05 |||serine protease |Schizosaccharomyces pombe|chr
2|||Manual
Length = 997
Score = 26.2 bits (55), Expect = 1.1
Identities = 19/53 (35%), Positives = 27/53 (50%)
Frame = -3
Query: 175 GVDAVLIGDDLPELSSNLVSALSGLDVHDFPHFDISELNKLLNTNIFCSARTT 17
G+D +IG+D E S L S L+ LD P++ I N NT + +A T
Sbjct: 176 GIDIRIIGNDAGEKLSILSSTLARLD-RPAPNYGIDNYND-FNTFYYQAASGT 226
>SPAC1805.16c |||purine nucleoside phosphorylase
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 315
Score = 26.2 bits (55), Expect = 1.1
Identities = 11/35 (31%), Positives = 19/35 (54%)
Frame = -3
Query: 166 AVLIGDDLPELSSNLVSALSGLDVHDFPHFDISEL 62
A++ G L L+S L + + + D PHF +S +
Sbjct: 44 AIICGSGLGTLASGLSAPVYEVPYEDIPHFHVSHV 78
>SPAC11E3.05 |||ubiquitin-protein ligase E3|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1323
Score = 25.8 bits (54), Expect = 1.4
Identities = 19/78 (24%), Positives = 28/78 (35%)
Frame = -3
Query: 283 EVDEDGSGHVLASGGLIVINIDXXXXXXXXXXVSTGGVDAVLIGDDLPELSSNLVSALSG 104
+VDE SG ++ IN++ S G + + SN S L
Sbjct: 911 DVDESNSGSIVFPKSKTSINLNNINSASASIMGSRFGAADIFMSKRPSTKGSNRPSILLS 970
Query: 103 LDVHDFPHFDISELNKLL 50
HDF I ++K L
Sbjct: 971 KPSHDFHVVRIISMSKYL 988
>SPCC74.02c |||mRNA cleavage and polyadenylation specificity factor
complex associated protein|Schizosaccharomyces pombe|chr
3|||Manual
Length = 710
Score = 24.6 bits (51), Expect = 3.3
Identities = 13/35 (37%), Positives = 19/35 (54%), Gaps = 1/35 (2%)
Frame = -3
Query: 181 TGGVDAVLIGDDLPE-LSSNLVSALSGLDVHDFPH 80
+G + + + DL E L L+SA GL+ H F H
Sbjct: 31 SGKPEFINLSPDLEENLDEKLMSAFPGLEPHVFQH 65
>SPAC30C2.07 |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 842
Score = 23.4 bits (48), Expect = 7.7
Identities = 10/24 (41%), Positives = 14/24 (58%)
Frame = +2
Query: 197 SDLQLERINVYYNEASGGKYVPRA 268
S L +E I+ Y A KY+PR+
Sbjct: 635 SFLDIENIDEYLESAENMKYLPRS 658
>SPCC1450.10c |||iron hydrogenase large subunit |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 538
Score = 23.4 bits (48), Expect = 7.7
Identities = 11/29 (37%), Positives = 16/29 (55%), Gaps = 1/29 (3%)
Frame = -1
Query: 177 VGSMPCSSEMISQN-LAPIWFPHCPAWMC 94
V S S+ I++N + PI CP W+C
Sbjct: 195 VHSNTSSNSGINENAVLPILSSSCPGWIC 223
>SPAPB1E7.04c |||chitinase |Schizosaccharomyces pombe|chr 1|||Manual
Length = 1236
Score = 23.4 bits (48), Expect = 7.7
Identities = 8/26 (30%), Positives = 21/26 (80%)
Frame = +3
Query: 147 SSPMSTASTPPVLTTATQTCSWSASM 224
SSP++++S+ P+ ++++ ++SAS+
Sbjct: 688 SSPVASSSSSPIPSSSSLVSTYSASL 713
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,119,538
Number of Sequences: 5004
Number of extensions: 18959
Number of successful extensions: 73
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 70
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 71
length of database: 2,362,478
effective HSP length: 63
effective length of database: 2,047,226
effective search space used: 77794588
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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