Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= S06A01NCLL0003_N14
(461 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC1F5.02 |||protein disulfide isomerase|Schizosaccharomyces po... 121 6e-29
SPAC13F5.05 |||thioredoxin family protein|Schizosaccharomyces po... 73 2e-14
SPAC17H9.14c |||protein disulfide isomerase|Schizosaccharomyces ... 63 2e-11
SPAC959.05c |||protein disulfide isomerase |Schizosaccharomyces ... 45 6e-06
SPBC3D6.13c |||protein disulfide isomerase |Schizosaccharomyces ... 41 8e-05
SPBC12D12.07c |trx2||mitochondrial thioredoxin Trx2|Schizosaccha... 41 8e-05
SPAC7D4.07c |trx1||cytosolic thioredoxin Trx1 |Schizosaccharomyc... 39 4e-04
SPBC577.08c |txl1|trx3|thioredoxin-like I protein Txl1|Schizosac... 36 0.004
SPBC26H8.06 |grx4||glutaredoxin Grx4|Schizosaccharomyces pombe|c... 28 0.60
SPAC2F3.12c |||conserved eukaryotic protein|Schizosaccharomyces ... 26 2.4
SPAC1D4.14 |tho2|SPAC22F3.14c|THO complex subunit Tho2 |Schizosa... 25 7.4
SPAC664.07c |rad9||checkpoint clamp complex protein Rad9|Schizos... 24 9.8
>SPAC1F5.02 |||protein disulfide isomerase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 492
Score = 121 bits (291), Expect = 6e-29
Identities = 61/146 (41%), Positives = 90/146 (61%), Gaps = 1/146 (0%)
Frame = +1
Query: 13 CSAAEEDVLDLTDSDFSSVLDQHDTALVMFYAPWCGHCKRLKPEYAVAAGILKKDDPPVS 192
C++AE V + + ++ +V FYAPWCGHCK L PEY AA L+KD +S
Sbjct: 19 CASAE--VPKVNKEGLNELITADKVLMVKFYAPWCGHCKALAPEYESAADELEKDG--IS 74
Query: 193 LAKVDCTEGGKSTCEKFSVSGYPTLKIFRKGELSSDYNGPRESNGIVKYMRAQVGPSSRD 372
L +VDCTE G C ++S+ GYPTL +F+ G+ S Y+GPR+ + +VKYMR Q+ P+ +
Sbjct: 75 LVEVDCTEEG-DLCSEYSIRGYPTLNVFKNGKQISQYSGPRKHDALVKYMRKQLLPTVKP 133
Query: 373 LLTLADFEAFTAK-DDVVVIGFFEKE 447
++ E F K DD+ V+ FF+ +
Sbjct: 134 -ISKDTLENFVEKADDLAVVAFFKDQ 158
Score = 69.7 bits (163), Expect = 2e-13
Identities = 37/90 (41%), Positives = 51/90 (56%), Gaps = 1/90 (1%)
Frame = +1
Query: 22 AEEDVLDLTDSDFSS-VLDQHDTALVMFYAPWCGHCKRLKPEYAVAAGILKKDDPPVSLA 198
++ED++ L +F V+D+ LV FYAPWCGHCK L P Y A DD V +A
Sbjct: 353 SQEDLVVLVADNFDDIVMDETKDVLVEFYAPWCGHCKNLAPTYEKLAEEY-SDDSNVVVA 411
Query: 199 KVDCTEGGKSTCEKFSVSGYPTLKIFRKGE 288
K+D TE S S+SG+PT+ F+ +
Sbjct: 412 KIDATENDISV----SISGFPTIMFFKAND 437
>SPAC13F5.05 |||thioredoxin family protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 363
Score = 73.3 bits (172), Expect = 2e-14
Identities = 41/123 (33%), Positives = 65/123 (52%), Gaps = 6/123 (4%)
Frame = +1
Query: 31 DVLDLTDSDFSSVLDQHDTALVMFYAPWCGHCKRLKPEYAVAAGILKKDDPPVSLAKVDC 210
+ ++L +F + +LV+FYAPWCG+CK+L P Y A L P + VDC
Sbjct: 32 NTIELNSKNFRKFVKAKGPSLVVFYAPWCGYCKKLVPTYQKLASNLHSLLP---VTAVDC 88
Query: 211 -TEGGKSTCEKFSVSGYPTLKIF---RKGE--LSSDYNGPRESNGIVKYMRAQVGPSSRD 372
+ ++ C ++ V G+PT+K+ KG S+DYNG R + K++ + PS
Sbjct: 89 DADQNRAVCSQYQVQGFPTIKLVYPSSKGSSLSSTDYNGDRSYKSLQKFVSDSI-PSKVK 147
Query: 373 LLT 381
+LT
Sbjct: 148 ILT 150
>SPAC17H9.14c |||protein disulfide isomerase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 359
Score = 62.9 bits (146), Expect = 2e-11
Identities = 35/112 (31%), Positives = 57/112 (50%), Gaps = 3/112 (2%)
Frame = +1
Query: 31 DVLDLTDSDFSSV-LDQHDTALVMFYAPWCGHCKRLKPEYAVAAGILKKDDPPVSLAKVD 207
+V++L +F V +D LV FYA WCG+CKRL P Y G + K++P V + K++
Sbjct: 141 NVVELDSLNFDKVVMDDKKDVLVEFYADWCGYCKRLAPTYE-TLGKVFKNEPNVEIVKIN 199
Query: 208 CTEGGKSTCEKFSVSGYPTLKIFRKGELSSD--YNGPRESNGIVKYMRAQVG 357
+ V+ +PT+K F K + Y G R +++Y+ + G
Sbjct: 200 -ADVFADIGRLHEVASFPTIKFFPKDDKDKPELYEGDRSLESLIEYINKKSG 250
Score = 60.5 bits (140), Expect = 1e-10
Identities = 31/98 (31%), Positives = 51/98 (52%), Gaps = 1/98 (1%)
Frame = +1
Query: 88 ALVMFYAPWCGHCKRLKPEYAVAAGILKKDDPPVSLAKVDCTEGGKSTCEKFSVSGYPTL 267
AL+ FYA WCGHCK L P Y G L +D V + K+D + +K+ ++G+PTL
Sbjct: 42 ALIEFYATWCGHCKSLAPVYE-ELGALFEDHNDVLIGKID-ADTHSDVADKYHITGFPTL 99
Query: 268 KIF-RKGELSSDYNGPRESNGIVKYMRAQVGPSSRDLL 378
F G Y+ R+ + + +++ + G R ++
Sbjct: 100 IWFPPDGSEPVQYSNARDVDSLTQFVSEKTGIKKRKIV 137
>SPAC959.05c |||protein disulfide isomerase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 632
Score = 44.8 bits (101), Expect = 6e-06
Identities = 28/94 (29%), Positives = 46/94 (48%)
Frame = +1
Query: 58 FSSVLDQHDTALVMFYAPWCGHCKRLKPEYAVAAGILKKDDPPVSLAKVDCTEGGKSTCE 237
F + + A VMF + HC+ AV + I + D + +A+V+C E K C
Sbjct: 189 FGNEIMSKTRAFVMFVS--LKHCEDCFHWEAVWSSITRNTDERLKMAQVNCDE-EKEMCN 245
Query: 238 KFSVSGYPTLKIFRKGELSSDYNGPRESNGIVKY 339
F + +PT ++F +G S YNGP + ++ Y
Sbjct: 246 HFHIKKFPTFRVF-QGFDSIQYNGPLKYQQLLSY 278
>SPBC3D6.13c |||protein disulfide isomerase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 726
Score = 41.1 bits (92), Expect = 8e-05
Identities = 27/112 (24%), Positives = 53/112 (47%), Gaps = 4/112 (3%)
Frame = +1
Query: 43 LTDSDFSSVLDQHDTALVMFYAPWCGHCKRLKP--EYAVAAGILKKDDPPVSLAKVDCTE 216
LTD+D S + + T + +Y P CG CKRL P + V + + +VDC++
Sbjct: 31 LTDNDLESEVSK-GTWFIKYYLPSCGACKRLGPMWDNMVEKAKEQVEGSNFHFGEVDCSK 89
Query: 217 GGKSTCEKFSVSGYPTLKIFRKGELSSD--YNGPRESNGIVKYMRAQVGPSS 366
S+C ++ PTL +++ GE+ + + ++ ++ + P +
Sbjct: 90 -ELSSCA--NIRAVPTLYLYQNGEIVEEVPFGASTSEASLLDFVETHLNPDT 138
Score = 39.1 bits (87), Expect = 3e-04
Identities = 28/134 (20%), Positives = 57/134 (42%)
Frame = +1
Query: 49 DSDFSSVLDQHDTALVMFYAPWCGHCKRLKPEYAVAAGILKKDDPPVSLAKVDCTEGGKS 228
D+D + L + + FY+ C C + + A ++ +++A ++C K
Sbjct: 288 DADIDAALTDKEGWFIQFYSSECDDCDDVSTAWYAMANRMRGK---LNVAHINCAVS-KR 343
Query: 229 TCEKFSVSGYPTLKIFRKGELSSDYNGPRESNGIVKYMRAQVGPSSRDLLTLADFEAFTA 408
C+++S+ +PT +F K E +Y G +V + R+ + L D
Sbjct: 344 ACKQYSIQYFPTF-LFFKEEAFVEYVGLPNEGDLVSFAEEAANFEIRE-VELLDTVNAEK 401
Query: 409 KDDVVVIGFFEKES 450
DV + F++ +S
Sbjct: 402 NGDVFFLYFYDDDS 415
>SPBC12D12.07c |trx2||mitochondrial thioredoxin
Trx2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 121
Score = 41.1 bits (92), Expect = 8e-05
Identities = 24/78 (30%), Positives = 37/78 (47%)
Frame = +1
Query: 55 DFSSVLDQHDTALVMFYAPWCGHCKRLKPEYAVAAGILKKDDPPVSLAKVDCTEGGKSTC 234
D+++ + +V FYA WCG CK LKP L + + S V+ +
Sbjct: 27 DYNTRISADKVTVVDFYADWCGPCKYLKPFLEK----LSEQNQKASFIAVN-ADKFSDIA 81
Query: 235 EKFSVSGYPTLKIFRKGE 288
+K V PT+ +FRKG+
Sbjct: 82 QKNGVYALPTMVLFRKGQ 99
>SPAC7D4.07c |trx1||cytosolic thioredoxin Trx1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 103
Score = 38.7 bits (86), Expect = 4e-04
Identities = 21/79 (26%), Positives = 37/79 (46%)
Frame = +1
Query: 52 SDFSSVLDQHDTALVMFYAPWCGHCKRLKPEYAVAAGILKKDDPPVSLAKVDCTEGGKST 231
S+F S++ Q +V F+A WCG CK + P++ + + KVD + +
Sbjct: 9 SEFKSIVCQDKLVVVDFFATWCGPCKAIAPKFEQFSNTYS----DATFIKVDVDQLSEIA 64
Query: 232 CEKFSVSGYPTLKIFRKGE 288
E V P+ +++ GE
Sbjct: 65 AEA-GVHAMPSFFLYKNGE 82
>SPBC577.08c |txl1|trx3|thioredoxin-like I protein
Txl1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 290
Score = 35.5 bits (78), Expect = 0.004
Identities = 20/62 (32%), Positives = 26/62 (41%)
Frame = +1
Query: 103 YAPWCGHCKRLKPEYAVAAGILKKDDPPVSLAKVDCTEGGKSTCEKFSVSGYPTLKIFRK 282
YA WCG CK + P ++ A K P AKV+ E + V PT F
Sbjct: 27 YADWCGPCKAISPLFSQLAS--KYASPKFVFAKVNVDE-QRQIASGLGVKAMPTFVFFEN 83
Query: 283 GE 288
G+
Sbjct: 84 GK 85
>SPBC26H8.06 |grx4||glutaredoxin Grx4|Schizosaccharomyces pombe|chr
2|||Manual
Length = 244
Score = 28.3 bits (60), Expect = 0.60
Identities = 18/73 (24%), Positives = 30/73 (41%)
Frame = +1
Query: 91 LVMFYAPWCGHCKRLKPEYAVAAGILKKDDPPVSLAKVDCTEGGKSTCEKFSVSGYPTLK 270
L+ FYAPW CK++ + A KD K++ E E F V+ P
Sbjct: 24 LLNFYAPWAAPCKQMNQVFDQFA----KDTKNAVFLKIE-AEKFSDIAESFDVNAVPLFV 78
Query: 271 IFRKGELSSDYNG 309
+ ++ + +G
Sbjct: 79 LIHGAKVLARISG 91
>SPAC2F3.12c |||conserved eukaryotic protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 279
Score = 26.2 bits (55), Expect = 2.4
Identities = 13/34 (38%), Positives = 17/34 (50%)
Frame = +1
Query: 223 KSTCEKFSVSGYPTLKIFRKGELSSDYNGPRESN 324
KS EK + Y LK+ + E + DYN P N
Sbjct: 37 KSPLEKEIANEYEALKVTERKEDTQDYNEPELHN 70
>SPAC1D4.14 |tho2|SPAC22F3.14c|THO complex subunit Tho2
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1628
Score = 24.6 bits (51), Expect = 7.4
Identities = 10/22 (45%), Positives = 13/22 (59%)
Frame = -1
Query: 257 YPDTENFSHVLLPPSVQSTFAN 192
YP+ N H LL S+ S +AN
Sbjct: 436 YPELSNLYHKLLHISISSIYAN 457
>SPAC664.07c |rad9||checkpoint clamp complex protein
Rad9|Schizosaccharomyces pombe|chr 1|||Manual
Length = 426
Score = 24.2 bits (50), Expect = 9.8
Identities = 11/25 (44%), Positives = 13/25 (52%)
Frame = +1
Query: 220 GKSTCEKFSVSGYPTLKIFRKGELS 294
G S C + V G P L F KG+ S
Sbjct: 265 GSSICAYYGVPGKPILLTFAKGKNS 289
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.317 0.135 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,835,689
Number of Sequences: 5004
Number of extensions: 35194
Number of successful extensions: 93
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 85
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 88
length of database: 2,362,478
effective HSP length: 67
effective length of database: 2,027,210
effective search space used: 174340060
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.6 bits)
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