BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= S06A01NCLL0003_M24
(415 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC970.05 |rpl3601|rpl36-1|60S ribosomal protein L36|Schizosacc... 58 4e-10
SPBC405.07 |rpl3602|rpl36-2, rpl36|60S ribosomal protein L36|Sch... 58 4e-10
SPBC36B7.09 |gcn2|ppk28, ppk28, SPBP18G5.01|eIF2 alpha kinase Gc... 31 0.094
SPBC119.05c |||Wiskott-Aldrich syndrome homolog binding protein ... 29 0.29
SPAC4F8.15 |itr1|SPAC7D4.01|myo-inositol transporter Itr1|Schizo... 27 1.2
SPBC3E7.12c |chr1|cfh4|chitin synthase regulatory factor |Schizo... 27 1.2
SPAC10F6.06 |vip1||RNA-binding protein Vip1|Schizosaccharomyces ... 26 2.0
SPCC1795.08c |||histone acetyltransferase complex subunit |Schiz... 25 3.5
SPAPB1A10.06c |||ATP-dependent RNA helicase Dhr1 |Schizosaccharo... 25 4.7
SPAC2F3.16 |||ubiquitin-protein ligase E3 |Schizosaccharomyces p... 25 6.1
SPBC646.02 |cwf11||complexed with Cdc5 protein Cwf11 |Schizosacc... 25 6.1
SPBC26H8.12 |||cytochrome c heme lyase|Schizosaccharomyces pombe... 25 6.1
SPCC553.04 |cyp9||WD repeat containing cyclophilin family peptid... 25 6.1
SPBC8D2.20c |sec31||COPII-coated vesicle component Sec31 |Schizo... 24 8.1
SPCC613.12c |raf1|dos1, cmc1, clr8|Rik1-associated factor Raf1|S... 24 8.1
>SPCC970.05 |rpl3601|rpl36-1|60S ribosomal protein
L36|Schizosaccharomyces pombe|chr 3|||Manual
Length = 99
Score = 58.4 bits (135), Expect = 4e-10
Identities = 30/57 (52%), Positives = 39/57 (68%)
Frame = +1
Query: 193 VEKFVGHAQYEKRAMELLKVSKDKRALKFLKRRLGTHIRAKRKREELSNVLTQMRKA 363
V + G A YE+R MEL++ S+DKRA K K+RLGT RAK K EEL++V+ R A
Sbjct: 42 VREVAGFAPYERRVMELIRNSQDKRARKLAKKRLGTLKRAKGKIEELTSVIQSSRLA 98
>SPBC405.07 |rpl3602|rpl36-2, rpl36|60S ribosomal protein
L36|Schizosaccharomyces pombe|chr 2|||Manual
Length = 99
Score = 58.4 bits (135), Expect = 4e-10
Identities = 30/57 (52%), Positives = 39/57 (68%)
Frame = +1
Query: 193 VEKFVGHAQYEKRAMELLKVSKDKRALKFLKRRLGTHIRAKRKREELSNVLTQMRKA 363
V + G A YE+R MEL++ S+DKRA K K+RLGT RAK K EEL++V+ R A
Sbjct: 42 VREVAGFAPYERRVMELIRNSQDKRARKLAKKRLGTLKRAKGKIEELTSVIQSSRLA 98
Score = 24.2 bits (50), Expect = 8.1
Identities = 14/29 (48%), Positives = 18/29 (62%), Gaps = 1/29 (3%)
Frame = +2
Query: 95 KGITRTK-PIKIRPARLKGIQTKHSKFVR 178
KG T TK + RP+R KG +K + FVR
Sbjct: 11 KGKTLTKRQLPERPSRRKGHLSKRTAFVR 39
>SPBC36B7.09 |gcn2|ppk28, ppk28, SPBP18G5.01|eIF2 alpha kinase Gcn2
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1576
Score = 30.7 bits (66), Expect = 0.094
Identities = 21/59 (35%), Positives = 30/59 (50%), Gaps = 1/59 (1%)
Frame = +1
Query: 205 VGHAQYEKRAMELLKVSKDKRALKFLKRRLGTHIRAK-RKREELSNVLTQMRKAAATPS 378
+ HA ++ L+VSKD KRR+ THI + +R LS V Q+R + PS
Sbjct: 1120 INHADILSSILDYLQVSKD-------KRRMATHILGQINQRLTLSQVRNQLRIESLVPS 1171
>SPBC119.05c |||Wiskott-Aldrich syndrome homolog binding protein
Lsb1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 296
Score = 29.1 bits (62), Expect = 0.29
Identities = 22/70 (31%), Positives = 34/70 (48%)
Frame = +1
Query: 175 PRILASVEKFVGHAQYEKRAMELLKVSKDKRALKFLKRRLGTHIRAKRKREELSNVLTQM 354
P+ +SVEK G AM L + S +KR + ++ G + A +K+ E S+V +
Sbjct: 66 PKRKSSVEKRAGSVASAVAAMSLSQNSGEKRTPEEPRKLPG--VPAPQKQSEASSVNSST 123
Query: 355 RKAAATPSPP 384
K PS P
Sbjct: 124 EKLPPPPSYP 133
>SPAC4F8.15 |itr1|SPAC7D4.01|myo-inositol transporter
Itr1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 575
Score = 27.1 bits (57), Expect = 1.2
Identities = 11/31 (35%), Positives = 18/31 (58%), Gaps = 1/31 (3%)
Frame = -3
Query: 389 WRGGLGVAAAFLICVSTLLSSSRF-LFARMC 300
W G LG+ A+FL +S + + F L+ +C
Sbjct: 488 WAGNLGIGASFLTLMSEITPTGTFALYGGLC 518
>SPBC3E7.12c |chr1|cfh4|chitin synthase regulatory factor
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 456
Score = 27.1 bits (57), Expect = 1.2
Identities = 11/29 (37%), Positives = 19/29 (65%), Gaps = 1/29 (3%)
Frame = -3
Query: 263 LSFDTLSNSIALFSYCACP-TNFSTEAKI 180
+S+D+LS+ +++ S+ CP T F T I
Sbjct: 79 MSYDSLSDDVSVLSFLDCPLTTFETAPSI 107
>SPAC10F6.06 |vip1||RNA-binding protein Vip1|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 257
Score = 26.2 bits (55), Expect = 2.0
Identities = 13/30 (43%), Positives = 16/30 (53%)
Frame = -3
Query: 227 FSYCACPTNFSTEAKIRGQT*SVWFECPLA 138
FS+C +N STE QT + FE P A
Sbjct: 23 FSFCGKVSNISTEKSGETQTAKIQFERPSA 52
>SPCC1795.08c |||histone acetyltransferase complex subunit
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 985
Score = 25.4 bits (53), Expect = 3.5
Identities = 11/36 (30%), Positives = 19/36 (52%)
Frame = +3
Query: 300 THPREEEA*RAQQRTNTDEEGGRNTKPTTPPLTLSE 407
T P E + + + +E T+PT PP+T++E
Sbjct: 235 TKPVEVATDQVESKEVKKKEVSETTEPTAPPVTVAE 270
>SPAPB1A10.06c |||ATP-dependent RNA helicase Dhr1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1183
Score = 25.0 bits (52), Expect = 4.7
Identities = 16/58 (27%), Positives = 33/58 (56%)
Frame = +1
Query: 181 ILASVEKFVGHAQYEKRAMELLKVSKDKRALKFLKRRLGTHIRAKRKREELSNVLTQM 354
IL V K + ++ +LLK ++ + K K+RL +I K K+EE+++++ ++
Sbjct: 56 ILIDVPKEERQKRKQELKDQLLKENEGSISSK-KKKRLDKYIENKLKKEEVASLIAKL 112
>SPAC2F3.16 |||ubiquitin-protein ligase E3 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 425
Score = 24.6 bits (51), Expect = 6.1
Identities = 9/29 (31%), Positives = 15/29 (51%)
Frame = -2
Query: 246 EQFHCPLLILCVPNKLFNGSQNSRTNLEC 160
+ FHC LC+P +FN + + +C
Sbjct: 241 DYFHCKTCGLCLPISVFNTHRCIERSTDC 269
>SPBC646.02 |cwf11||complexed with Cdc5 protein Cwf11
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1284
Score = 24.6 bits (51), Expect = 6.1
Identities = 11/37 (29%), Positives = 19/37 (51%)
Frame = -3
Query: 296 PRRRFKNLRARLSFDTLSNSIALFSYCACPTNFSTEA 186
P ++ AR F+T+ ++ C P+N STE+
Sbjct: 658 PNAGLNSIYARNLFNTVEQLQSVLPNCHVPSNLSTES 694
>SPBC26H8.12 |||cytochrome c heme lyase|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 377
Score = 24.6 bits (51), Expect = 6.1
Identities = 8/20 (40%), Positives = 11/20 (55%)
Frame = +1
Query: 43 RNWSAKRPQNDKDIRWQKGY 102
+NW PQ + W+KGY
Sbjct: 219 KNWEYPSPQQMYNAMWRKGY 238
>SPCC553.04 |cyp9||WD repeat containing cyclophilin family
peptidyl-prolyl cis-trans isomerase
Cyp9|Schizosaccharomyces pombe|chr 3|||Manual
Length = 610
Score = 24.6 bits (51), Expect = 6.1
Identities = 12/33 (36%), Positives = 17/33 (51%)
Frame = -2
Query: 132 GLILMGFVRVIPFLPADIFVVLWPFRRPIAISN 34
G IL+G +I DI + L+P P A+ N
Sbjct: 449 GNILLGKAAIIHTTQGDISIKLYPEEAPKAVQN 481
>SPBC8D2.20c |sec31||COPII-coated vesicle component Sec31
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1224
Score = 24.2 bits (50), Expect = 8.1
Identities = 11/33 (33%), Positives = 20/33 (60%)
Frame = -3
Query: 266 RLSFDTLSNSIALFSYCACPTNFSTEAKIRGQT 168
R++ +L +++FS + T+ S EA I+G T
Sbjct: 318 RVAVASLEGKVSIFSIQSTNTDKSQEASIKGAT 350
>SPCC613.12c |raf1|dos1, cmc1, clr8|Rik1-associated factor
Raf1|Schizosaccharomyces pombe|chr 3|||Manual
Length = 638
Score = 24.2 bits (50), Expect = 8.1
Identities = 10/26 (38%), Positives = 16/26 (61%)
Frame = -1
Query: 358 SSSVLVRC*ALHASSSRGCVCRGDAS 281
S V++ + H S++RGC+ DAS
Sbjct: 406 SDKVIIGYDSFHTSNNRGCLALFDAS 431
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,698,308
Number of Sequences: 5004
Number of extensions: 33198
Number of successful extensions: 123
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 120
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 123
length of database: 2,362,478
effective HSP length: 66
effective length of database: 2,032,214
effective search space used: 144287194
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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