Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= S06A01NCLL0003_M23
(514 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 271 4e-74
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 266 1e-72
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 141 6e-35
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 105 5e-24
SPAC3F10.06c |||initiator methionine tRNA 2'-O-ribosyl phosphate... 29 0.31
SPBC2D10.16 |||sequence orphan|Schizosaccharomyces pombe|chr 2||... 26 2.9
SPAC1486.04c |alm1||medial ring protein Alm1|Schizosaccharomyces... 26 2.9
SPCC18B5.08c |||isoleucine-tRNA ligase|Schizosaccharomyces pombe... 26 2.9
SPBC1826.01c |mot1||TATA-binding protein associated factor Mot1|... 26 3.8
SPAC1805.15c |pub2||ubiquitin-protein ligase Pub2|Schizosaccharo... 25 8.8
SPAC521.04c |||calcium permease |Schizosaccharomyces pombe|chr 1... 25 8.8
>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 455
Score = 271 bits (665), Expect = 4e-74
Identities = 121/168 (72%), Positives = 145/168 (86%)
Frame = +3
Query: 9 IGKEIVDLVLDRIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEF 188
+GKE++D VL+RIR++AD C+GLQGFL+FH LL+ERL+++YGKKS L+F
Sbjct: 114 VGKEMIDSVLERIRRMADNCSGLQGFLVFHSFGGGTGSGLGALLLERLNMEYGKKSNLQF 173
Query: 189 AIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNR 368
++YPAPQVST+VVEPYNS+LTTH TL++SDC FMVDNEA YDICRRNLDIERPTY NLNR
Sbjct: 174 SVYPAPQVSTSVVEPYNSVLTTHATLDNSDCTFMVDNEACYDICRRNLDIERPTYENLNR 233
Query: 369 LIGQIVSSITASLRFDGALNVDLTEFQTNLVP*PRIHFPLVTYAPVIS 512
LI Q+VSSITASLRF G+LNVDL EFQTNLVP PRIHFPLVTY+P++S
Sbjct: 234 LIAQVVSSITASLRFAGSLNVDLNEFQTNLVPYPRIHFPLVTYSPIVS 281
>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 449
Score = 266 bits (652), Expect = 1e-72
Identities = 119/168 (70%), Positives = 144/168 (85%)
Frame = +3
Query: 9 IGKEIVDLVLDRIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEF 188
+GKE+VD V D+IR++AD C+GLQGFL+FH LL+ERL+++Y KKSKL+F
Sbjct: 110 VGKELVDEVTDKIRRIADNCSGLQGFLVFHSFGGGTGSGFGALLLERLAMEYTKKSKLQF 169
Query: 189 AIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNR 368
++YPAPQVST+VVEPYNS+LTTH TL+ +DC FMVDNE+ YDICRRNLDIERP+Y NLNR
Sbjct: 170 SVYPAPQVSTSVVEPYNSVLTTHATLDLADCTFMVDNESCYDICRRNLDIERPSYENLNR 229
Query: 369 LIGQIVSSITASLRFDGALNVDLTEFQTNLVP*PRIHFPLVTYAPVIS 512
LI Q+VSSITASLRF+G+LNVDL EFQTNLVP PRIHFPLVTYAP++S
Sbjct: 230 LIAQVVSSITASLRFEGSLNVDLAEFQTNLVPYPRIHFPLVTYAPIVS 277
>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 448
Score = 141 bits (341), Expect = 6e-35
Identities = 65/165 (39%), Positives = 98/165 (59%)
Frame = +3
Query: 12 GKEIVDLVLDRIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFA 191
G E+ D VLD +R+ A+ C LQGF + H LL+ ++ +Y + F+
Sbjct: 109 GAELADAVLDVVRREAEACDALQGFQLTHSLGGGTGSGMGTLLLSKIREEYPDRMMATFS 168
Query: 192 IYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRL 371
+ PAP+ S VVEPYN+ L+ H +E+SD F +DNEA+ I L I+ P+Y +LN L
Sbjct: 169 VAPAPKSSDTVVEPYNATLSMHQLVENSDETFCIDNEALSSIFANTLKIKSPSYDDLNHL 228
Query: 372 IGQIVSSITASLRFDGALNVDLTEFQTNLVP*PRIHFPLVTYAPV 506
+ +++ +T S RF G LN DL + N+VP PR+HF +V +AP+
Sbjct: 229 VSAVMAGVTTSFRFPGELNSDLRKLAVNMVPFPRLHFFMVGFAPL 273
>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 446
Score = 105 bits (251), Expect = 5e-24
Identities = 54/164 (32%), Positives = 87/164 (53%), Gaps = 1/164 (0%)
Frame = +3
Query: 15 KEIVDLVLDRIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAI 194
+ I + ++D I + AD L+GF + H L+ERL+ Y KK +++
Sbjct: 112 ERIFEDIMDMIDREADGSDSLEGFSLLHSIAGGTGSGLGSFLLERLNDRYPKKIIQTYSV 171
Query: 195 YPAPQ-VSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRL 371
+P Q VS VV+PYNS+L ++D ++DN A+ I L + PT+ N+L
Sbjct: 172 FPNSQSVSDVVVQPYNSLLALKRLTLNADSVVVLDNAALAHIAADRLHTQNPTFHQQNQL 231
Query: 372 IGQIVSSITASLRFDGALNVDLTEFQTNLVP*PRIHFPLVTYAP 503
+ ++S+ T +LR+ G +N DL +L+P PR HF L +Y P
Sbjct: 232 VSTVMSASTTTLRYPGYMNNDLVSIIASLIPSPRCHFLLTSYTP 275
>SPAC3F10.06c |||initiator methionine tRNA 2'-O-ribosyl phosphate
transferase |Schizosaccharomyces pombe|chr 1|||Manual
Length = 453
Score = 29.5 bits (63), Expect = 0.31
Identities = 11/38 (28%), Positives = 18/38 (47%)
Frame = +1
Query: 277 IVLSWSTMKPSMTSAAVTWILNARPTPTSIVLSARSYH 390
++ S+ T KP + TW +N R P S+ + H
Sbjct: 37 VIASYPTFKPVVNERCGTWYVNRRHAPISVYFKSTDGH 74
>SPBC2D10.16 |||sequence orphan|Schizosaccharomyces pombe|chr
2|||Manual
Length = 135
Score = 26.2 bits (55), Expect = 2.9
Identities = 12/32 (37%), Positives = 19/32 (59%)
Frame = -3
Query: 431 DIEGAVETQRGGNR*YDLADKTIEVGVGRAFN 336
DIE E GN+ ++L +K I++ G+ FN
Sbjct: 58 DIEAFAEYALDGNKYFNLINKIIDMQDGKRFN 89
>SPAC1486.04c |alm1||medial ring protein Alm1|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1727
Score = 26.2 bits (55), Expect = 2.9
Identities = 20/73 (27%), Positives = 34/73 (46%), Gaps = 1/73 (1%)
Frame = -3
Query: 248 KDRVVGLYNSSGHLGCGIDSELQFRLLAVVNGESLHEERSETRTGATTERVEDEESL-EA 72
KD++ L N + HLG + S Q + E + + +++ RV++E + E
Sbjct: 146 KDQIEALQNENSHLGEQVQSAHQAL-------SDIEERKKQHMFASSSSRVKEEILVQEK 198
Query: 71 CALVSEFADAVED 33
ALVS+ A D
Sbjct: 199 SALVSDLASLQSD 211
>SPCC18B5.08c |||isoleucine-tRNA ligase|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 973
Score = 26.2 bits (55), Expect = 2.9
Identities = 16/60 (26%), Positives = 25/60 (41%)
Frame = +1
Query: 289 WSTMKPSMTSAAVTWILNARPTPTSIVLSARSYHRLPPLCVSTAPSMSI*RSSRPTWCPS 468
W T KP + A W ++ + + + S +PP S A + + RP WC S
Sbjct: 428 WRTHKPLILRATPQWFISLENERKTAIKALDSVKMIPP--NSRARLLGF-LNGRPEWCIS 484
>SPBC1826.01c |mot1||TATA-binding protein associated factor
Mot1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1953
Score = 25.8 bits (54), Expect = 3.8
Identities = 13/21 (61%), Positives = 13/21 (61%)
Frame = +1
Query: 334 ILNARPTPTSIVLSARSYHRL 396
I NAR T V S RSYHRL
Sbjct: 1499 IKNARAKLTKAVKSLRSYHRL 1519
>SPAC1805.15c |pub2||ubiquitin-protein ligase
Pub2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 671
Score = 24.6 bits (51), Expect = 8.8
Identities = 9/23 (39%), Positives = 13/23 (56%)
Frame = +2
Query: 185 VRYLSRTPGVHCCCRALQLYPYN 253
V ++S+ P H C L + PYN
Sbjct: 627 VGHISQLPKAHTCFNRLDIPPYN 649
>SPAC521.04c |||calcium permease |Schizosaccharomyces pombe|chr
1|||Manual
Length = 881
Score = 24.6 bits (51), Expect = 8.8
Identities = 11/31 (35%), Positives = 15/31 (48%)
Frame = -3
Query: 167 AVVNGESLHEERSETRTGATTERVEDEESLE 75
AVVNG S H + + + + ESLE
Sbjct: 662 AVVNGNSQHHHNRDDASSGLSSNGSENESLE 692
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,198,549
Number of Sequences: 5004
Number of extensions: 43154
Number of successful extensions: 159
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 155
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 158
length of database: 2,362,478
effective HSP length: 68
effective length of database: 2,022,206
effective search space used: 206265012
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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