Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= S06A01NCLL0003_M04
(560 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 320 1e-88
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 318 4e-88
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 159 2e-40
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 100 2e-22
SPAC3F10.06c |||initiator methionine tRNA 2'-O-ribosyl phosphate... 29 0.35
SPCC18.03 |||shuttle craft like transcriptional regulator|Schizo... 26 3.3
SPBC2D10.16 |||sequence orphan|Schizosaccharomyces pombe|chr 2||... 26 3.3
SPCC1442.04c |||conserved fungal protein|Schizosaccharomyces pom... 26 4.4
SPCC18B5.08c |||isoleucine-tRNA ligase|Schizosaccharomyces pombe... 26 4.4
SPBC1826.01c |mot1||TATA-binding protein associated factor Mot1|... 26 4.4
SPCC16C4.09 |sts5|orb4|RNB-like protein|Schizosaccharomyces pomb... 25 5.8
SPBC29A10.16c |||cytochrome b5 |Schizosaccharomyces pombe|chr 2|... 25 7.6
>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 455
Score = 320 bits (785), Expect = 1e-88
Identities = 142/183 (77%), Positives = 163/183 (89%)
Frame = +1
Query: 10 DYGKKSKLEFAIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDI 189
+YGKKS L+F++YPAPQVST+VVEPYNS+LTTH TL++SDC FMVDNEA YDICRRNLDI
Sbjct: 164 EYGKKSNLQFSVYPAPQVSTSVVEPYNSVLTTHATLDNSDCTFMVDNEACYDICRRNLDI 223
Query: 190 ERPTYTNLNRLIGQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLVTYAPVISAE 369
ERPTY NLNRLI Q+VSSITASLRF G+LNVDL EFQTNLVPYPRIHFPLVTY+P++SA
Sbjct: 224 ERPTYENLNRLIAQVVSSITASLRFAGSLNVDLNEFQTNLVPYPRIHFPLVTYSPIVSAA 283
Query: 370 KAYHEQLSVAEITNACFEPANQMVNCDPRHGKYMACCMLYRGDVVPNDVNAAIATIKTKR 549
KA+HE SV EITN CFEP NQMV CDPR G+YMA C+LYRGDV+P DV AA+ +IK++R
Sbjct: 284 KAFHESNSVQEITNQCFEPYNQMVKCDPRTGRYMATCLLYRGDVIPRDVQAAVTSIKSRR 343
Query: 550 TIQ 558
TIQ
Sbjct: 344 TIQ 346
>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 449
Score = 318 bits (781), Expect = 4e-88
Identities = 142/183 (77%), Positives = 162/183 (88%)
Frame = +1
Query: 10 DYGKKSKLEFAIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDI 189
+Y KKSKL+F++YPAPQVST+VVEPYNS+LTTH TL+ +DC FMVDNE+ YDICRRNLDI
Sbjct: 160 EYTKKSKLQFSVYPAPQVSTSVVEPYNSVLTTHATLDLADCTFMVDNESCYDICRRNLDI 219
Query: 190 ERPTYTNLNRLIGQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLVTYAPVISAE 369
ERP+Y NLNRLI Q+VSSITASLRF+G+LNVDL EFQTNLVPYPRIHFPLVTYAP++SA
Sbjct: 220 ERPSYENLNRLIAQVVSSITASLRFEGSLNVDLAEFQTNLVPYPRIHFPLVTYAPIVSAA 279
Query: 370 KAYHEQLSVAEITNACFEPANQMVNCDPRHGKYMACCMLYRGDVVPNDVNAAIATIKTKR 549
KA+HE SV EITN CFEP NQMV CDPR G+YMA C+LYRGDV+P DV AA+ TIK KR
Sbjct: 280 KAFHESNSVQEITNQCFEPYNQMVKCDPRAGRYMATCLLYRGDVIPRDVQAAVTTIKAKR 339
Query: 550 TIQ 558
TIQ
Sbjct: 340 TIQ 342
>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 448
Score = 159 bits (387), Expect = 2e-40
Identities = 72/183 (39%), Positives = 113/183 (61%)
Frame = +1
Query: 4 RGDYGKKSKLEFAIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNL 183
R +Y + F++ PAP+ S VVEPYN+ L+ H +E+SD F +DNEA+ I L
Sbjct: 156 REEYPDRMMATFSVAPAPKSSDTVVEPYNATLSMHQLVENSDETFCIDNEALSSIFANTL 215
Query: 184 DIERPTYTNLNRLIGQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLVTYAPVIS 363
I+ P+Y +LN L+ +++ +T S RF G LN DL + N+VP+PR+HF +V +AP+ +
Sbjct: 216 KIKSPSYDDLNHLVSAVMAGVTTSFRFPGELNSDLRKLAVNMVPFPRLHFFMVGFAPLAA 275
Query: 364 AEKAYHEQLSVAEITNACFEPANQMVNCDPRHGKYMACCMLYRGDVVPNDVNAAIATIKT 543
+ + +SV E+T F+ N MV DPRHG+Y+ L+RG V +V+ I +++T
Sbjct: 276 IGSSSFQAVSVPELTQQMFDANNMMVAADPRHGRYLTVAALFRGKVSMKEVDEQIRSVQT 335
Query: 544 KRT 552
K +
Sbjct: 336 KNS 338
>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 446
Score = 100 bits (239), Expect = 2e-22
Identities = 55/184 (29%), Positives = 98/184 (53%), Gaps = 5/184 (2%)
Frame = +1
Query: 13 YGKKSKLEFAIYPAPQ-VSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDI 189
Y KK ++++P Q VS VV+PYNS+L ++D ++DN A+ I L
Sbjct: 161 YPKKIIQTYSVFPNSQSVSDVVVQPYNSLLALKRLTLNADSVVVLDNAALAHIAADRLHT 220
Query: 190 ERPTYTNLNRLIGQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLVTYAPVIS-- 363
+ PT+ N+L+ ++S+ T +LR+ G +N DL +L+P PR HF L +Y P +
Sbjct: 221 QNPTFHQQNQLVSTVMSASTTTLRYPGYMNNDLVSIIASLIPSPRCHFLLTSYTPFTNQQ 280
Query: 364 -AEKAYHEQLSVAEITNACFEPANQMVNCDP-RHGKYMACCMLYRGDVVPNDVNAAIATI 537
E + +V ++ P NQMV+ +P + +++ + +G+ P DV+ ++ I
Sbjct: 281 VEEAKAIRKTTVLDVMRRLLLPKNQMVSVNPSKKSCFISILDIIQGEADPADVHKSLLRI 340
Query: 538 KTKR 549
+ +R
Sbjct: 341 RERR 344
>SPAC3F10.06c |||initiator methionine tRNA 2'-O-ribosyl phosphate
transferase |Schizosaccharomyces pombe|chr 1|||Manual
Length = 453
Score = 29.5 bits (63), Expect = 0.35
Identities = 11/38 (28%), Positives = 18/38 (47%)
Frame = +2
Query: 128 IVLSWSTMKPSMTSAAVTWILNARPTPTSIVLSARSYH 241
++ S+ T KP + TW +N R P S+ + H
Sbjct: 37 VIASYPTFKPVVNERCGTWYVNRRHAPISVYFKSTDGH 74
>SPCC18.03 |||shuttle craft like transcriptional
regulator|Schizosaccharomyces pombe|chr 3|||Manual
Length = 1077
Score = 26.2 bits (55), Expect = 3.3
Identities = 13/42 (30%), Positives = 17/42 (40%)
Frame = +1
Query: 403 ITNACFEPANQMVNCDPRHGKYMACCMLYRGDVVPNDVNAAI 528
+ ACFEP N C H K C L + D N ++
Sbjct: 361 LCGACFEPINAKCYCG-LHSKTYPCSSLPSPSISKKDENGSV 401
>SPBC2D10.16 |||sequence orphan|Schizosaccharomyces pombe|chr
2|||Manual
Length = 135
Score = 26.2 bits (55), Expect = 3.3
Identities = 12/32 (37%), Positives = 19/32 (59%)
Frame = -3
Query: 282 DIEGAVETQRGGNR*YDLADKTIEVGVGRAFN 187
DIE E GN+ ++L +K I++ G+ FN
Sbjct: 58 DIEAFAEYALDGNKYFNLINKIIDMQDGKRFN 89
>SPCC1442.04c |||conserved fungal protein|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 409
Score = 25.8 bits (54), Expect = 4.4
Identities = 29/127 (22%), Positives = 51/127 (40%), Gaps = 1/127 (0%)
Frame = +1
Query: 73 VVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLIGQIVSSITA 252
V E +I + L SD +NE YDI +N + E N I + + +
Sbjct: 47 VEEVVEAINGSPDLLSESDVENAKNNEHTYDIDNKNFNFEPKNSVNKTETIREELDQAAS 106
Query: 253 SLRFDGALNVDLTE-FQTNLVPYPRIHFPLVTYAPVISAEKAYHEQLSVAEITNACFEPA 429
+ + L++ E F+ +V T A + A+ H + + E+ E A
Sbjct: 107 KILIE-TLDLHQKEGFEETIVTEE------ATDAVDLEAKGTTHGENDLDEVPTELNELA 159
Query: 430 NQMVNCD 450
N++V+ D
Sbjct: 160 NEVVDVD 166
>SPCC18B5.08c |||isoleucine-tRNA ligase|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 973
Score = 25.8 bits (54), Expect = 4.4
Identities = 15/58 (25%), Positives = 24/58 (41%)
Frame = +2
Query: 140 WSTMKPSMTSAAVTWILNARPTPTSIVLSARSYHRLPPLCVSTAPSMSI*RSSRPTWC 313
W T KP + A W ++ + + + S +PP S A + + RP WC
Sbjct: 428 WRTHKPLILRATPQWFISLENERKTAIKALDSVKMIPP--NSRARLLGF-LNGRPEWC 482
>SPBC1826.01c |mot1||TATA-binding protein associated factor
Mot1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1953
Score = 25.8 bits (54), Expect = 4.4
Identities = 13/21 (61%), Positives = 13/21 (61%)
Frame = +2
Query: 185 ILNARPTPTSIVLSARSYHRL 247
I NAR T V S RSYHRL
Sbjct: 1499 IKNARAKLTKAVKSLRSYHRL 1519
>SPCC16C4.09 |sts5|orb4|RNB-like protein|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 1066
Score = 25.4 bits (53), Expect = 5.8
Identities = 11/27 (40%), Positives = 15/27 (55%)
Frame = -3
Query: 534 GSDSRVDVIGHDVTAVQHAAGHVFAVT 454
G+D + GH V +Q A G VF+ T
Sbjct: 421 GADEKPKYAGHVVAVLQRAPGQVFSGT 447
>SPBC29A10.16c |||cytochrome b5 |Schizosaccharomyces pombe|chr
2|||Manual
Length = 124
Score = 25.0 bits (52), Expect = 7.6
Identities = 14/50 (28%), Positives = 20/50 (40%)
Frame = -3
Query: 534 GSDSRVDVIGHDVTAVQHAAGHVFAVTRVTVHHLVGGLEASVRDLRYREL 385
G D +D G D T GH A + +G L+ + R +EL
Sbjct: 41 GLDIMLDYAGQDATKAYQDIGHSIAADELLEEMYIGDLKPGTEE-RLKEL 89
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,325,062
Number of Sequences: 5004
Number of extensions: 47633
Number of successful extensions: 147
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 144
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 147
length of database: 2,362,478
effective HSP length: 69
effective length of database: 2,017,202
effective search space used: 236012634
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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