Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= S06A01NCLL0003_E10
(472 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|ch... 86 2e-18
SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces pomb... 82 5e-17
SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces... 76 2e-15
SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr 3|||Ma... 50 2e-07
SPAC926.03 |rlc1||myosin II regulatory light chain |Schizosaccha... 48 7e-07
SPAC1687.14c |||EF hand family protein, unknown role|Schizosacch... 28 0.83
SPAC1565.07c |||TATA binding protein interacting protein |Schizo... 25 4.4
SPAC1687.05 |pli1||SUMO E3 ligase Pli1|Schizosaccharomyces pombe... 25 5.8
>SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|chr
1|||Manual
Length = 150
Score = 86.2 bits (204), Expect = 2e-18
Identities = 45/117 (38%), Positives = 71/117 (60%), Gaps = 2/117 (1%)
Frame = +1
Query: 127 SEDQLAEFQEAFQLFDSRGDGKIHVAQIGDALRALGQNPTESDVKKFTLHLKPDER--IS 300
+++Q+AEF+EAF LFD DG I ++G +R+LGQ+PT ++++ + D I
Sbjct: 7 TDEQIAEFREAFSLFDRDQDGNITSNELGVVMRSLGQSPTAAELQDMINEVDADGNGTID 66
Query: 301 FEVFLPIYQAISKARSGDTANDFIEGLRHFDKDGNGFISSAELRHLLSTLGEKLSDD 471
F FL + K + D + E + FDKDGNG+I+ EL H+L++LGE+LS +
Sbjct: 67 FTEFLTM--MARKMKDTDNEEEVREAFKVFDKDGNGYITVEELTHVLTSLGERLSQE 121
Score = 33.5 bits (73), Expect = 0.017
Identities = 15/42 (35%), Positives = 26/42 (61%)
Frame = +1
Query: 133 DQLAEFQEAFQLFDSRGDGKIHVAQIGDALRALGQNPTESDV 258
D E +EAF++FD G+G I V ++ L +LG+ ++ +V
Sbjct: 82 DNEEEVREAFKVFDKDGNGYITVEELTHVLTSLGERLSQEEV 123
>SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 141
Score = 81.8 bits (193), Expect = 5e-17
Identities = 43/108 (39%), Positives = 64/108 (59%)
Frame = +1
Query: 148 FQEAFQLFDSRGDGKIHVAQIGDALRALGQNPTESDVKKFTLHLKPDERISFEVFLPIYQ 327
+++AF LFD G G+I IGD LRA GQNPT +++ + L P E + E FL +
Sbjct: 8 YKQAFSLFDRHGTGRIPKTSIGDLLRACGQNPTLAEITEIESTL-PAE-VDMEQFLQVLN 65
Query: 328 AISKARSGDTANDFIEGLRHFDKDGNGFISSAELRHLLSTLGEKLSDD 471
+ +F++G + FDKD G I ELR++L++LGEKLS++
Sbjct: 66 RPNGFDMPGDPEEFVKGFQVFDKDATGMIGVGELRYVLTSLGEKLSNE 113
Score = 27.5 bits (58), Expect = 1.1
Identities = 12/38 (31%), Positives = 21/38 (55%)
Frame = +1
Query: 145 EFQEAFQLFDSRGDGKIHVAQIGDALRALGQNPTESDV 258
EF + FQ+FD G I V ++ L +LG+ + ++
Sbjct: 78 EFVKGFQVFDKDATGMIGVGELRYVLTSLGEKLSNEEM 115
>SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 143
Score = 76.2 bits (179), Expect = 2e-15
Identities = 41/115 (35%), Positives = 68/115 (59%)
Frame = +1
Query: 127 SEDQLAEFQEAFQLFDSRGDGKIHVAQIGDALRALGQNPTESDVKKFTLHLKPDERISFE 306
S++Q E +EAF L+D DG I + +G LR+LG N T++++ K + L + I +
Sbjct: 4 SKEQTDEMKEAFVLYDIDKDGLIPTSHVGSVLRSLGINVTDAELAKLSNEL--GDAIDEK 61
Query: 307 VFLPIYQAISKARSGDTANDFIEGLRHFDKDGNGFISSAELRHLLSTLGEKLSDD 471
F+ +K R ++ ++I+ R FDKD +G+I +A+ + TLGEKLSD+
Sbjct: 62 KFMSFVS--NKLRETESEEEYIKAFRVFDKDNSGYIETAKFADYMKTLGEKLSDN 114
Score = 35.9 bits (79), Expect = 0.003
Identities = 16/65 (24%), Positives = 35/65 (53%)
Frame = +1
Query: 145 EFQEAFQLFDSRGDGKIHVAQIGDALRALGQNPTESDVKKFTLHLKPDERISFEVFLPIY 324
E+ +AF++FD G I A+ D ++ LG+ ++++V+ P SF+ + +
Sbjct: 79 EYIKAFRVFDKDNSGYIETAKFADYMKTLGEKLSDNEVQLMVQEADPTNSGSFDYYDFVQ 138
Query: 325 QAISK 339
+ ++K
Sbjct: 139 RIMAK 143
>SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr
3|||Manual
Length = 176
Score = 49.6 bits (113), Expect = 2e-07
Identities = 35/116 (30%), Positives = 51/116 (43%), Gaps = 2/116 (1%)
Frame = +1
Query: 127 SEDQLAEFQEAFQLFDSRGDGKIHVAQIGDALRALGQNPTESDVKKFTLHLKPDER--IS 300
+E+Q + EAF+LFDS D I ++ A+RALG N +S+V K + +
Sbjct: 32 TEEQRQDINEAFKLFDSDKDNAIDYHELRAAMRALGFNAEKSEVLKILRDFDKTGKGYLQ 91
Query: 301 FEVFLPIYQAISKARSGDTANDFIEGLRHFDKDGNGFISSAELRHLLSTLGEKLSD 468
E F+ + K D + FD D G IS LR + L E + D
Sbjct: 92 MEDFVRV--MTEKIVERDPLEEIKRAFELFDDDETGKISLRNLRRVAKELNENIDD 145
Score = 30.7 bits (66), Expect = 0.12
Identities = 18/65 (27%), Positives = 31/65 (47%), Gaps = 2/65 (3%)
Frame = +1
Query: 133 DQLAEFQEAFQLFDSRGDGKIHVAQIGDALRALGQNPTESDVKKF--TLHLKPDERISFE 306
D L E + AF+LFD GKI + + + L +N + +++ L D I+ +
Sbjct: 107 DPLEEIKRAFELFDDDETGKISLRNLRRVAKELNENIDDQELEAMIEEFDLDQDGEINEQ 166
Query: 307 VFLPI 321
F+ I
Sbjct: 167 EFIAI 171
>SPAC926.03 |rlc1||myosin II regulatory light chain
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 184
Score = 48.0 bits (109), Expect = 7e-07
Identities = 32/115 (27%), Positives = 53/115 (46%)
Frame = +1
Query: 118 AGYSEDQLAEFQEAFQLFDSRGDGKIHVAQIGDALRALGQNPTESDVKKFTLHLKPDERI 297
A + Q+ E +EAF L D GDG I + L +L Q+ +E + + P I
Sbjct: 40 AQLTSSQIQELKEAFALLDKDGDGNIGREDVKTMLTSLNQDASEDSINHMFESINPP--I 97
Query: 298 SFEVFLPIYQAISKARSGDTANDFIEGLRHFDKDGNGFISSAELRHLLSTLGEKL 462
+ FL ++ S ND +E FD +G I + +R LS++G+++
Sbjct: 98 NLAAFLTAMGSMLCRIS--PRNDLLEAFSTFDDTQSGKIPISTMRDALSSMGDRM 150
Score = 30.3 bits (65), Expect = 0.15
Identities = 16/45 (35%), Positives = 24/45 (53%), Gaps = 4/45 (8%)
Frame = +1
Query: 154 EAFQLFDSRGDGKIHVAQIGDALRALGQ----NPTESDVKKFTLH 276
EAF FD GKI ++ + DAL ++G ES ++ +T H
Sbjct: 121 EAFSTFDDTQSGKIPISTMRDALSSMGDRMDPQEVESILRSYTSH 165
Score = 27.1 bits (57), Expect = 1.4
Identities = 11/33 (33%), Positives = 20/33 (60%)
Frame = +1
Query: 373 EGLRHFDKDGNGFISSAELRHLLSTLGEKLSDD 471
E DKDG+G I +++ +L++L + S+D
Sbjct: 52 EAFALLDKDGDGNIGREDVKTMLTSLNQDASED 84
>SPAC1687.14c |||EF hand family protein, unknown
role|Schizosaccharomyces pombe|chr 1|||Manual
Length = 76
Score = 27.9 bits (59), Expect = 0.83
Identities = 18/64 (28%), Positives = 30/64 (46%)
Frame = +1
Query: 145 EFQEAFQLFDSRGDGKIHVAQIGDALRALGQNPTESDVKKFTLHLKPDERISFEVFLPIY 324
E +EAF LFD G I + + LG+N T+ ++ + ++S E F ++
Sbjct: 13 EAEEAFDLFDVTHKGYIDFEDLRRSCAQLGENLTKEQLQLMLDLAGTNGKVSREEFAELW 72
Query: 325 QAIS 336
IS
Sbjct: 73 IHIS 76
>SPAC1565.07c |||TATA binding protein interacting protein
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1220
Score = 25.4 bits (53), Expect = 4.4
Identities = 17/55 (30%), Positives = 30/55 (54%), Gaps = 3/55 (5%)
Frame = +1
Query: 304 EVFLPIYQAISKARSGDTANDFI--EGLRHFDKDGNGFISS-AELRHLLSTLGEK 459
++F+ + I+K +G + I + RH DK GN F ++ EL +L +G+K
Sbjct: 643 DIFMSVTD-ITKIENGTKIYEEILQDCCRHIDKSGNEFTTAYLELLEVLLKVGQK 696
>SPAC1687.05 |pli1||SUMO E3 ligase Pli1|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 727
Score = 25.0 bits (52), Expect = 5.8
Identities = 15/55 (27%), Positives = 26/55 (47%)
Frame = +1
Query: 199 VAQIGDALRALGQNPTESDVKKFTLHLKPDERISFEVFLPIYQAISKARSGDTAN 363
+ Q+ D LR G + + + T + ERI+ E ++A+ KA GD +
Sbjct: 23 IPQLKDILRVFGLRLSGTKAELITRIKQLIERIAIENNTTSWEALKKAIDGDVTS 77
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,982,409
Number of Sequences: 5004
Number of extensions: 38547
Number of successful extensions: 114
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 100
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 108
length of database: 2,362,478
effective HSP length: 67
effective length of database: 2,027,210
effective search space used: 180421690
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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