Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= S06A01NCLL0003_E05
(429 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC1223.08c |dfr1||dihydrofolate reductase Dfr1|Schizosaccharom... 69 4e-13
SPAC22A12.06c |||serine hydrolase|Schizosaccharomyces pombe|chr ... 52 4e-08
SPAC23A1.12c |||phenylalanine-tRNA ligase beta subunit |Schizosa... 29 0.40
SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1... 26 2.2
SPAC30D11.01c ||SPAC56F8.01|alpha-glucosidase|Schizosaccharomyce... 26 2.2
SPBPB10D8.02c |||arylsulfatase |Schizosaccharomyces pombe|chr 2|... 26 2.2
SPBC16H5.03c |fub2|uba2|SUMO E1-like activator enzyme Fub2|Schiz... 25 6.6
SPAC3H1.11 |hsr1||transcription factor Hsr1|Schizosaccharomyces ... 24 8.7
SPCC550.10 |meu8||betaine aldehyde dehydrogenase |Schizosaccharo... 24 8.7
>SPCC1223.08c |dfr1||dihydrofolate reductase
Dfr1|Schizosaccharomyces pombe|chr 3|||Manual
Length = 461
Score = 68.5 bits (160), Expect = 4e-13
Identities = 52/161 (32%), Positives = 81/161 (50%), Gaps = 23/161 (14%)
Frame = +1
Query: 13 HGYRQNGALYRGKIGSFRKAVGKYAQLTFLSAPHKVVNEDG--------------GGDED 150
HG+ Q+G ++ K+GS +K + KYA+L F + P V +E+ GG+++
Sbjct: 11 HGWIQSGPVFSKKMGSVQKYLSKYAELHFPTGP-VVADEEADPNDEEEKKRLAALGGEQN 69
Query: 151 AMSW-WFNAED--NTFSGKCLGGPAIGFEETLRLIEKVVKDHGPFDGFMGFSQCACLVGL 321
+ WF ED NT+ ++E+L I + +++ GPFDG +GFSQ A + +
Sbjct: 70 GGKFGWFEVEDFKNTYG---------SWDESLECINQYMQEKGPFDGLIGFSQGAGIGAM 120
Query: 322 LAAMQQKGYLPY------SFKFAIFASGFRSGSLLHKGFYD 426
LA M Q G P FKF +F GFR+ FY+
Sbjct: 121 LAQMLQPGQPPNPYVQHPPFKFVVFVGGFRAEKPEFDHFYN 161
>SPAC22A12.06c |||serine hydrolase|Schizosaccharomyces pombe|chr
1|||Manual
Length = 429
Score = 52.0 bits (119), Expect = 4e-08
Identities = 38/140 (27%), Positives = 64/140 (45%), Gaps = 14/140 (10%)
Frame = +1
Query: 13 HGYRQNGALYRGKIGSFRKAVGKYAQLTFLSAP---HKVVNEDGGGDEDAMSWWFNAEDN 183
HGY ++G L+ K+ + R+ + F + P K +E G DA+S F++
Sbjct: 13 HGYAESGELFSVKLRALRERMADSVDFYFPTGPIELDKAKDELNGSGFDALSTVFSSSPA 72
Query: 184 TFS------GKCLGGPAIGFEETLRLIEKVVKDHGPFDGFMGFSQCACLVGLLAAM---- 333
+ + + + + +K+HGPFDG +GFSQ L LAA+
Sbjct: 73 SHRRGWWRINEYADTKQLEPTKAFEYLASYIKEHGPFDGILGFSQGTNLAANLAALVTIP 132
Query: 334 -QQKGYLPYSFKFAIFASGF 390
Q+ + F+FA+F SG+
Sbjct: 133 KYQEYFSQPPFRFALFFSGY 152
>SPAC23A1.12c |||phenylalanine-tRNA ligase beta subunit
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 589
Score = 28.7 bits (61), Expect = 0.40
Identities = 15/58 (25%), Positives = 29/58 (50%), Gaps = 2/58 (3%)
Frame = +1
Query: 34 ALYRGKIGSFRKAVGKYAQLTFLSAPHKVVNEDGGGDEDAMSWWFNAEDNT--FSGKC 201
A++ G F + G ++ + +++N D DA+ +W AED++ F G+C
Sbjct: 488 AIFAGLNSGFEQIHGLLDRVMLMLNTKRIMNPK---DSDAVGYWIEAEDDSTFFPGRC 542
>SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr
1|||Manual
Length = 574
Score = 26.2 bits (55), Expect = 2.2
Identities = 11/26 (42%), Positives = 17/26 (65%)
Frame = -2
Query: 242 SLKVSSKPIAGPPRHFPLNVLSSALN 165
S K S+ P+ PP + ++ L+SALN
Sbjct: 529 STKTSNPPVEAPPSNNLMDALASALN 554
>SPAC30D11.01c ||SPAC56F8.01|alpha-glucosidase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 993
Score = 26.2 bits (55), Expect = 2.2
Identities = 17/47 (36%), Positives = 23/47 (48%), Gaps = 7/47 (14%)
Frame = +1
Query: 286 MGFSQCACLVGLLAAMQQKGYL-------PYSFKFAIFASGFRSGSL 405
+G+ A G + +QQ GY PYS A+ +GF SGSL
Sbjct: 853 LGYVNIAVRGGNIIPLQQPGYTTYESRNNPYSLLIAMDNNGFASGSL 899
>SPBPB10D8.02c |||arylsulfatase |Schizosaccharomyces pombe|chr
2|||Manual
Length = 554
Score = 26.2 bits (55), Expect = 2.2
Identities = 11/24 (45%), Positives = 14/24 (58%)
Frame = +2
Query: 38 YIAEKLVHSVKPLENMHSLHSYLP 109
Y AEKL+ +K E S +YLP
Sbjct: 192 YFAEKLIDQLKNREKSQSFFAYLP 215
>SPBC16H5.03c |fub2|uba2|SUMO E1-like activator enzyme
Fub2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 628
Score = 24.6 bits (51), Expect = 6.6
Identities = 9/17 (52%), Positives = 10/17 (58%)
Frame = -2
Query: 326 ANRPTRQAHCENPMNPS 276
A RPTR HCE P+
Sbjct: 411 AKRPTRVLHCEKTCKPN 427
>SPAC3H1.11 |hsr1||transcription factor Hsr1|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 582
Score = 24.2 bits (50), Expect = 8.7
Identities = 12/31 (38%), Positives = 14/31 (45%)
Frame = -2
Query: 107 ADKNVSCAYFPTALRNEPIFPRYKAPFCLYP 15
A NV+C FPT + N P P L P
Sbjct: 255 AQPNVNCENFPTTVPNYPFQQPSYNPNALVP 285
>SPCC550.10 |meu8||betaine aldehyde dehydrogenase
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 500
Score = 24.2 bits (50), Expect = 8.7
Identities = 9/16 (56%), Positives = 11/16 (68%)
Frame = +1
Query: 301 CACLVGLLAAMQQKGY 348
C C+VG L +QKGY
Sbjct: 368 CKCVVGGLPRSEQKGY 383
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,882,384
Number of Sequences: 5004
Number of extensions: 38639
Number of successful extensions: 91
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 89
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 90
length of database: 2,362,478
effective HSP length: 66
effective length of database: 2,032,214
effective search space used: 154448264
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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