BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= S06A01NCLL0003_C17
(459 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 248 3e-67
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 243 9e-66
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 128 5e-31
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 97 1e-21
SPAC3F10.06c |||initiator methionine tRNA 2'-O-ribosyl phosphate... 29 0.26
SPBC2D10.16 |||sequence orphan|Schizosaccharomyces pombe|chr 2||... 26 2.4
SPCC18B5.08c |||isoleucine-tRNA ligase|Schizosaccharomyces pombe... 26 3.2
SPBC1826.01c |mot1||TATA-binding protein associated factor Mot1|... 26 3.2
SPAC1486.04c |alm1||medial ring protein Alm1|Schizosaccharomyces... 25 5.6
SPAC1805.15c |pub2||ubiquitin-protein ligase Pub2|Schizosaccharo... 25 7.3
SPAC521.04c |||calcium permease |Schizosaccharomyces pombe|chr 1... 25 7.3
SPBC651.01c |nog1|SPBC725.18c|GTP binding protein Nog1 |Schizosa... 24 9.7
SPBC582.05c |brc1||BRCT domain protein Brc1|Schizosaccharomyces ... 24 9.7
SPCC962.06c |bpb1|sf1|zinc finger protein Bpb1|Schizosaccharomyc... 24 9.7
SPBC21.06c |cdc7|pld1, its10|serine/threonine protein kinase Cdc... 24 9.7
SPBC1921.01c |rpl3701|rpl37-1, rpl37|60S ribosomal protein L37|S... 24 9.7
>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 455
Score = 248 bits (607), Expect = 3e-67
Identities = 111/150 (74%), Positives = 129/150 (86%)
Frame = +1
Query: 10 DRIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAPQVST 189
+RIR++AD C+GLQGFL+FH LL+ERL+++YGKKS L+F++YPAPQVST
Sbjct: 124 ERIRRMADNCSGLQGFLVFHSFGGGTGSGLGALLLERLNMEYGKKSNLQFSVYPAPQVST 183
Query: 190 AVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLIGQIVSSIT 369
+VVEPYNS+LTTH TL++SDC FMVDNEA YDICRRNLDIERPTY NLNRLI Q+VSSIT
Sbjct: 184 SVVEPYNSVLTTHATLDNSDCTFMVDNEACYDICRRNLDIERPTYENLNRLIAQVVSSIT 243
Query: 370 ASLRFDGALNVDLTEFQTNLVPYPRIHFPL 459
ASLRF G+LNVDL EFQTNLVPYPRIHFPL
Sbjct: 244 ASLRFAGSLNVDLNEFQTNLVPYPRIHFPL 273
>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 449
Score = 243 bits (595), Expect = 9e-66
Identities = 108/150 (72%), Positives = 129/150 (86%)
Frame = +1
Query: 10 DRIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAPQVST 189
D+IR++AD C+GLQGFL+FH LL+ERL+++Y KKSKL+F++YPAPQVST
Sbjct: 120 DKIRRIADNCSGLQGFLVFHSFGGGTGSGFGALLLERLAMEYTKKSKLQFSVYPAPQVST 179
Query: 190 AVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLIGQIVSSIT 369
+VVEPYNS+LTTH TL+ +DC FMVDNE+ YDICRRNLDIERP+Y NLNRLI Q+VSSIT
Sbjct: 180 SVVEPYNSVLTTHATLDLADCTFMVDNESCYDICRRNLDIERPSYENLNRLIAQVVSSIT 239
Query: 370 ASLRFDGALNVDLTEFQTNLVPYPRIHFPL 459
ASLRF+G+LNVDL EFQTNLVPYPRIHFPL
Sbjct: 240 ASLRFEGSLNVDLAEFQTNLVPYPRIHFPL 269
>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 448
Score = 128 bits (308), Expect = 5e-31
Identities = 57/148 (38%), Positives = 87/148 (58%)
Frame = +1
Query: 10 DRIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAPQVST 189
D +R+ A+ C LQGF + H LL+ ++ +Y + F++ PAP+ S
Sbjct: 118 DVVRREAEACDALQGFQLTHSLGGGTGSGMGTLLLSKIREEYPDRMMATFSVAPAPKSSD 177
Query: 190 AVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLIGQIVSSIT 369
VVEPYN+ L+ H +E+SD F +DNEA+ I L I+ P+Y +LN L+ +++ +T
Sbjct: 178 TVVEPYNATLSMHQLVENSDETFCIDNEALSSIFANTLKIKSPSYDDLNHLVSAVMAGVT 237
Query: 370 ASLRFDGALNVDLTEFQTNLVPYPRIHF 453
S RF G LN DL + N+VP+PR+HF
Sbjct: 238 TSFRFPGELNSDLRKLAVNMVPFPRLHF 265
>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 446
Score = 97.1 bits (231), Expect = 1e-21
Identities = 51/151 (33%), Positives = 79/151 (52%), Gaps = 1/151 (0%)
Frame = +1
Query: 10 DRIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAPQ-VS 186
D I + AD L+GF + H L+ERL+ Y KK ++++P Q VS
Sbjct: 120 DMIDREADGSDSLEGFSLLHSIAGGTGSGLGSFLLERLNDRYPKKIIQTYSVFPNSQSVS 179
Query: 187 TAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLIGQIVSSI 366
VV+PYNS+L ++D ++DN A+ I L + PT+ N+L+ ++S+
Sbjct: 180 DVVVQPYNSLLALKRLTLNADSVVVLDNAALAHIAADRLHTQNPTFHQQNQLVSTVMSAS 239
Query: 367 TASLRFDGALNVDLTEFQTNLVPYPRIHFPL 459
T +LR+ G +N DL +L+P PR HF L
Sbjct: 240 TTTLRYPGYMNNDLVSIIASLIPSPRCHFLL 270
>SPAC3F10.06c |||initiator methionine tRNA 2'-O-ribosyl phosphate
transferase |Schizosaccharomyces pombe|chr 1|||Manual
Length = 453
Score = 29.5 bits (63), Expect = 0.26
Identities = 11/38 (28%), Positives = 18/38 (47%)
Frame = +2
Query: 248 IVLSWSTMKPSMTSAAVTWILNARPTPTSIVLSARSYH 361
++ S+ T KP + TW +N R P S+ + H
Sbjct: 37 VIASYPTFKPVVNERCGTWYVNRRHAPISVYFKSTDGH 74
>SPBC2D10.16 |||sequence orphan|Schizosaccharomyces pombe|chr
2|||Manual
Length = 135
Score = 26.2 bits (55), Expect = 2.4
Identities = 12/32 (37%), Positives = 19/32 (59%)
Frame = -1
Query: 402 DIEGAVETQRGGNR*YDLADKTIEVGVGRAFN 307
DIE E GN+ ++L +K I++ G+ FN
Sbjct: 58 DIEAFAEYALDGNKYFNLINKIIDMQDGKRFN 89
>SPCC18B5.08c |||isoleucine-tRNA ligase|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 973
Score = 25.8 bits (54), Expect = 3.2
Identities = 15/58 (25%), Positives = 24/58 (41%)
Frame = +2
Query: 260 WSTMKPSMTSAAVTWILNARPTPTSIVLSARSYHRLPPLCVSTAPSMSI*RSSRPTWC 433
W T KP + A W ++ + + + S +PP S A + + RP WC
Sbjct: 428 WRTHKPLILRATPQWFISLENERKTAIKALDSVKMIPP--NSRARLLGF-LNGRPEWC 482
>SPBC1826.01c |mot1||TATA-binding protein associated factor
Mot1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1953
Score = 25.8 bits (54), Expect = 3.2
Identities = 13/21 (61%), Positives = 13/21 (61%)
Frame = +2
Query: 305 ILNARPTPTSIVLSARSYHRL 367
I NAR T V S RSYHRL
Sbjct: 1499 IKNARAKLTKAVKSLRSYHRL 1519
>SPAC1486.04c |alm1||medial ring protein Alm1|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1727
Score = 25.0 bits (52), Expect = 5.6
Identities = 19/68 (27%), Positives = 33/68 (48%), Gaps = 1/68 (1%)
Frame = -1
Query: 219 KDRVVGLYNSSGHLGCGIDSELQFRLLAVVNGESLHEERSETRTGATTERVEDEESL-EA 43
KD++ L N + HLG + S Q + E + + +++ RV++E + E
Sbjct: 146 KDQIEALQNENSHLGEQVQSAHQAL-------SDIEERKKQHMFASSSSRVKEEILVQEK 198
Query: 42 CALVSEFA 19
ALVS+ A
Sbjct: 199 SALVSDLA 206
>SPAC1805.15c |pub2||ubiquitin-protein ligase
Pub2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 671
Score = 24.6 bits (51), Expect = 7.3
Identities = 9/23 (39%), Positives = 13/23 (56%)
Frame = +3
Query: 156 VRYLSRTPGVHCCCRALQLYPYN 224
V ++S+ P H C L + PYN
Sbjct: 627 VGHISQLPKAHTCFNRLDIPPYN 649
>SPAC521.04c |||calcium permease |Schizosaccharomyces pombe|chr
1|||Manual
Length = 881
Score = 24.6 bits (51), Expect = 7.3
Identities = 11/31 (35%), Positives = 15/31 (48%)
Frame = -1
Query: 138 AVVNGESLHEERSETRTGATTERVEDEESLE 46
AVVNG S H + + + + ESLE
Sbjct: 662 AVVNGNSQHHHNRDDASSGLSSNGSENESLE 692
>SPBC651.01c |nog1|SPBC725.18c|GTP binding protein Nog1
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 642
Score = 24.2 bits (50), Expect = 9.7
Identities = 13/48 (27%), Positives = 23/48 (47%)
Frame = -1
Query: 168 IDSELQFRLLAVVNGESLHEERSETRTGATTERVEDEESLEACALVSE 25
+D E++ +LLA+ E E + E ++EE LE + + E
Sbjct: 445 VDPEIEAKLLALDEEEERLEREGYYDSDQEIEDADEEEVLEKASRIRE 492
>SPBC582.05c |brc1||BRCT domain protein Brc1|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 878
Score = 24.2 bits (50), Expect = 9.7
Identities = 9/32 (28%), Positives = 16/32 (50%)
Frame = +1
Query: 346 GQIVSSITASLRFDGALNVDLTEFQTNLVPYP 441
G ++ + L+ +G L +D +TN P P
Sbjct: 500 GSTITGVNRELQDEGRLEIDAKSSKTNTPPSP 531
>SPCC962.06c |bpb1|sf1|zinc finger protein Bpb1|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 587
Score = 24.2 bits (50), Expect = 9.7
Identities = 16/56 (28%), Positives = 27/56 (48%)
Frame = +2
Query: 263 STMKPSMTSAAVTWILNARPTPTSIVLSARSYHRLPPLCVSTAPSMSI*RSSRPTW 430
ST S TS+ W A+P ++ + + + P +AP++S+ SS P W
Sbjct: 413 STSVSSSTSSPAPW---AKPASSAAPSNPAPWQQ--PAAPQSAPALSMNPSSLPPW 463
>SPBC21.06c |cdc7|pld1, its10|serine/threonine protein kinase
Cdc7|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1062
Score = 24.2 bits (50), Expect = 9.7
Identities = 10/16 (62%), Positives = 11/16 (68%)
Frame = -2
Query: 215 IEL*GSTTAVDTWGAG 168
IEL G+TTA D W G
Sbjct: 176 IELVGATTASDIWSVG 191
>SPBC1921.01c |rpl3701|rpl37-1, rpl37|60S ribosomal protein
L37|Schizosaccharomyces pombe|chr 2|||Manual
Length = 108
Score = 24.2 bits (50), Expect = 9.7
Identities = 12/26 (46%), Positives = 14/26 (53%)
Frame = +2
Query: 296 VTWILNARPTPTSIVLSARSYHRLPP 373
V W +RP S V+ AR H LPP
Sbjct: 67 VIWGTVSRPHGNSGVVRARFTHNLPP 92
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,964,851
Number of Sequences: 5004
Number of extensions: 37360
Number of successful extensions: 137
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 134
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 136
length of database: 2,362,478
effective HSP length: 67
effective length of database: 2,027,210
effective search space used: 172312850
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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