Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= S06A01NCLL0002_M22
(531 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC1556.01c |rad50|SPAP4C9.01c|DNA repair protein Rad50|Schizos... 134 6e-33
SPAC31A2.05c |mis4||cohesin loading factor Mis4|Schizosaccharomy... 28 0.76
SPAC1952.16 |rga9||RhoGAp, GTPase activator towards Rho/Rac/Cdc4... 27 1.3
SPCC162.08c |nup211||nuclear pore complex associated protein|Sch... 27 1.7
SPAC222.07c |hri2||eIF2 alpha kinase Hri2|Schizosaccharomyces po... 27 1.7
SPAC10F6.10 |||protein kinase, RIO family |Schizosaccharomyces p... 27 2.3
SPAC1006.04c |mcp3|mug7|sequence orphan|Schizosaccharomyces pomb... 26 3.0
SPBC14C8.02 |tim44||TIM23 translocase complex subunit Tim44|Schi... 26 3.0
SPBC13E7.05 |gpi14||pig-M|Schizosaccharomyces pombe|chr 2|||Manual 26 4.0
SPAC19E9.02 |fin1||serine/threonine protein kinase Fin1|Schizosa... 26 4.0
SPAC23D3.10c |eng2||endo-1,3-beta-glucanase Eng2|Schizosaccharom... 25 5.3
SPBC3D6.07 |gpi3||pig-A|Schizosaccharomyces pombe|chr 2|||Manual 25 5.3
SPBC18E5.10 |||iron sulfur cluster assembly protein |Schizosacch... 25 7.0
SPCC5E4.06 |smc6|rad18|Smc5-6 complex SMC subunit Smc6|Schizosac... 25 9.3
SPAC29A4.08c |prp19|cwf8|ubiquitin-protein ligase E4 |Schizosacc... 25 9.3
SPBC28F2.12 |rpb1||DNA-directed RNA polymerase II large subunit|... 25 9.3
SPBC1734.05c |spf31||DNAJ protein Spf31|Schizosaccharomyces pomb... 25 9.3
SPBC36B7.09 |gcn2|ppk28, ppk28, SPBP18G5.01|eIF2 alpha kinase Gc... 25 9.3
SPAC4A8.05c |myp2|myo3|myosin II heavy chain |Schizosaccharomyce... 25 9.3
>SPAC1556.01c |rad50|SPAP4C9.01c|DNA repair protein
Rad50|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1290
Score = 134 bits (325), Expect = 6e-33
Identities = 72/175 (41%), Positives = 104/175 (59%)
Frame = +2
Query: 5 EALISQQTKLFREKATMEGRLSELKTTLKTFRQELKKSLTKDIEKTFREKIYELHVTKAI 184
+ L S+ L E+A + G +L+ ++ ++EL KD ++ FR ++ + T
Sbjct: 1045 QILKSKYGALNAERAGLLGECKQLENSITKDKEELNMEF-KDADERFRRQLIKTKTTGKA 1103
Query: 185 DKDIRDYAVALEKCLIEFHREKMENINLIIRELWRKIYRGNDIDYIEIKTEGSMNIDSER 364
++D+ YA AL+ +++ H KM IN I+ ELW++ Y G DID I I+++
Sbjct: 1104 NEDLGKYAKALDVAIMQLHSMKMNEINRIVDELWKQTYCGTDIDTILIRSDSE---GKGN 1160
Query: 365 RKYDYRVVQSKNGMEIDMRGRCSAGQKV*ACLIIRLALAETFSSRFGVLALDEPT 529
R Y+YRV K E+DMRGRCSAGQKV AC+IIRLALAE G+LALDEPT
Sbjct: 1161 RTYNYRVCMVKGDAELDMRGRCSAGQKVLACIIIRLALAECLGVNCGILALDEPT 1215
>SPAC31A2.05c |mis4||cohesin loading factor Mis4|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1583
Score = 28.3 bits (60), Expect = 0.76
Identities = 24/99 (24%), Positives = 41/99 (41%)
Frame = -3
Query: 352 NVHATFSFNLDIINIIPSVNLSPEFPNNKVNIFHLLPVEFNQTLFQCNGVISYVFVYGFG 173
N+ A +FN DII+ IPS+ ++N L V FN F C G IS + +
Sbjct: 657 NIPAPTNFN-DIISFIPSIT--------RLN--ELSQVSFNHFYFLCKGDISLENILPYN 705
Query: 172 YMKLIDLFSESLFNILSQALFQFLPECFECSLEFTKPSF 56
Y K + + + C + +++ ++ F
Sbjct: 706 YNKWFSFLLQLRKVCNDSEALKIIDNCIDKNMQKSQEGF 744
>SPAC1952.16 |rga9||RhoGAp, GTPase activator towards
Rho/Rac/Cdc42-like small GTPases|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 684
Score = 27.5 bits (58), Expect = 1.3
Identities = 21/73 (28%), Positives = 34/73 (46%)
Frame = +2
Query: 65 LSELKTTLKTFRQELKKSLTKDIEKTFREKIYELHVTKAIDKDIRDYAVALEKCLIEFHR 244
LS + +L+ E SL + + + IY + T D D R Y++ +KCL+ H
Sbjct: 510 LSSITCSLRLCLLEFPDSLIRSSFYDYFKAIYTTY-TDFEDLDHRLYSI--KKCLLHLHS 566
Query: 245 EKMENINLIIREL 283
+ + IIR L
Sbjct: 567 TPLHILEEIIRHL 579
>SPCC162.08c |nup211||nuclear pore complex associated
protein|Schizosaccharomyces pombe|chr 3|||Manual
Length = 1837
Score = 27.1 bits (57), Expect = 1.7
Identities = 21/65 (32%), Positives = 33/65 (50%)
Frame = +2
Query: 32 LFREKATMEGRLSELKTTLKTFRQELKKSLTKDIEKTFREKIYELHVTKAIDKDIRDYAV 211
L +EK +E +L E TF+Q++ SL +++E +E V +A K+ R A
Sbjct: 1364 LEKEKQELETKLQETAKETDTFKQQV-NSLNEEVENLKKE------VEQANTKNTRLAAA 1416
Query: 212 ALEKC 226
EKC
Sbjct: 1417 WNEKC 1421
>SPAC222.07c |hri2||eIF2 alpha kinase Hri2|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 639
Score = 27.1 bits (57), Expect = 1.7
Identities = 16/48 (33%), Positives = 27/48 (56%), Gaps = 1/48 (2%)
Frame = -3
Query: 487 CFCESQSYNKTRSNFLTSATSATHIDLHT-IFRLNNSIVVFAAFRVNV 347
CF + +SY R++FL+ I LHT +FR+ + V++ VN+
Sbjct: 366 CFDDLESYLIRRNHFLSFPLCKEQIQLHTDLFRMIINGVMYVHEGVNL 413
>SPAC10F6.10 |||protein kinase, RIO family |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 521
Score = 26.6 bits (56), Expect = 2.3
Identities = 15/63 (23%), Positives = 29/63 (46%)
Frame = +2
Query: 5 EALISQQTKLFREKATMEGRLSELKTTLKTFRQELKKSLTKDIEKTFREKIYELHVTKAI 184
E ++ +T++ K G +SE+ + T ++ T + K F KIY+ +
Sbjct: 123 EQVLDPRTRMILLKLINNGTISEINGCISTGKEANVYHATNEDGKHFAIKIYKTSILVFK 182
Query: 185 DKD 193
D+D
Sbjct: 183 DRD 185
>SPAC1006.04c |mcp3|mug7|sequence orphan|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 952
Score = 26.2 bits (55), Expect = 3.0
Identities = 17/52 (32%), Positives = 25/52 (48%)
Frame = +2
Query: 5 EALISQQTKLFREKATMEGRLSELKTTLKTFRQELKKSLTKDIEKTFREKIY 160
E IS+ ++ REKA + S + QE +S KD+E FR+ Y
Sbjct: 398 ELKISRLSESLREKAGLLEFQSSVSEQRDLLYQEQIQSSIKDMENVFRKNEY 449
>SPBC14C8.02 |tim44||TIM23 translocase complex subunit
Tim44|Schizosaccharomyces pombe|chr 2|||Manual
Length = 427
Score = 26.2 bits (55), Expect = 3.0
Identities = 14/32 (43%), Positives = 19/32 (59%)
Frame = +2
Query: 23 QTKLFREKATMEGRLSELKTTLKTFRQELKKS 118
QT +F + A + S +K + TFR ELKKS
Sbjct: 22 QTSIFSQNAAPQ---SPIKVFMDTFRAELKKS 50
>SPBC13E7.05 |gpi14||pig-M|Schizosaccharomyces pombe|chr 2|||Manual
Length = 815
Score = 25.8 bits (54), Expect = 4.0
Identities = 11/28 (39%), Positives = 17/28 (60%)
Frame = -3
Query: 160 IDLFSESLFNILSQALFQFLPECFECSL 77
++L+ ES F + +LF FLP+ C L
Sbjct: 680 LNLYYESSFGAKASSLFAFLPQLSLCML 707
>SPAC19E9.02 |fin1||serine/threonine protein kinase
Fin1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 722
Score = 25.8 bits (54), Expect = 4.0
Identities = 17/65 (26%), Positives = 35/65 (53%), Gaps = 3/65 (4%)
Frame = +2
Query: 2 HEALISQQTKL-FREKATMEGRLSELKTTLKTFRQELKKSLTKDIEKTFR--EKIYELHV 172
H+ LI + L FRE+ + R SEL+ + + + ++ L +++EK R + Y+ H+
Sbjct: 305 HQMLIQLENDLQFREQR-LSARESELENVIASRLAQREEILRRELEKQLRDMDARYQRHM 363
Query: 173 TKAID 187
++
Sbjct: 364 QTVVN 368
>SPAC23D3.10c |eng2||endo-1,3-beta-glucanase
Eng2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 706
Score = 25.4 bits (53), Expect = 5.3
Identities = 9/15 (60%), Positives = 11/15 (73%)
Frame = -2
Query: 254 PSSPCGIQSNTFPVQ 210
PS P G+ SN FP+Q
Sbjct: 22 PSPPLGLTSNLFPIQ 36
>SPBC3D6.07 |gpi3||pig-A|Schizosaccharomyces pombe|chr 2|||Manual
Length = 456
Score = 25.4 bits (53), Expect = 5.3
Identities = 13/63 (20%), Positives = 31/63 (49%), Gaps = 1/63 (1%)
Frame = -3
Query: 496 GAKCFCESQSY-NKTRSNFLTSATSATHIDLHTIFRLNNSIVVFAAFRVNVHATFSFNLD 320
G K + +Y ++ +LT+ + ++ LHT++R F+ F + + N++
Sbjct: 28 GHKVIVITHAYKDRVGVRYLTNGLTVYYVPLHTVYRETTFPSFFSFFPIFRNIVIRENIE 87
Query: 319 IIN 311
I++
Sbjct: 88 IVH 90
>SPBC18E5.10 |||iron sulfur cluster assembly protein
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 452
Score = 25.0 bits (52), Expect = 7.0
Identities = 10/30 (33%), Positives = 19/30 (63%)
Frame = +2
Query: 182 IDKDIRDYAVALEKCLIEFHREKMENINLI 271
I KD + V ++KC+I+ + +K+ NL+
Sbjct: 156 IRKDYYEETVMMQKCIIQAYAKKLLGKNLL 185
>SPCC5E4.06 |smc6|rad18|Smc5-6 complex SMC subunit
Smc6|Schizosaccharomyces pombe|chr 3|||Manual
Length = 1140
Score = 24.6 bits (51), Expect = 9.3
Identities = 18/72 (25%), Positives = 38/72 (52%), Gaps = 2/72 (2%)
Frame = +2
Query: 44 KATMEGRLSELKTTLKTFRQELK--KSLTKDIEKTFREKIYELHVTKAIDKDIRDYAVAL 217
++ ++G++S + + + E KS +DI KTF E++ +DI++ A
Sbjct: 366 QSDIDGKISSKEEVIGRAKGETDTTKSKFEDIVKTFDGYRSEMNDVDIQKRDIQNSINAA 425
Query: 218 EKCLIEFHREKM 253
+ CL + +RE++
Sbjct: 426 KSCL-DVYREQL 436
>SPAC29A4.08c |prp19|cwf8|ubiquitin-protein ligase E4
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 488
Score = 24.6 bits (51), Expect = 9.3
Identities = 15/37 (40%), Positives = 20/37 (54%)
Frame = +2
Query: 104 ELKKSLTKDIEKTFREKIYELHVTKAIDKDIRDYAVA 214
+LK SL +EKTF+E + TK K D AV+
Sbjct: 157 QLKTSLRSTVEKTFQELSSKRKRTKLQPKWATDDAVS 193
>SPBC28F2.12 |rpb1||DNA-directed RNA polymerase II large
subunit|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1752
Score = 24.6 bits (51), Expect = 9.3
Identities = 9/15 (60%), Positives = 11/15 (73%)
Frame = -3
Query: 79 LEFTKPSFHCGFLSE 35
+E KP FH GFLS+
Sbjct: 86 IELAKPVFHIGFLSK 100
>SPBC1734.05c |spf31||DNAJ protein Spf31|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 209
Score = 24.6 bits (51), Expect = 9.3
Identities = 10/21 (47%), Positives = 14/21 (66%)
Frame = +3
Query: 384 LFNLKMVWRSICVADVALVRK 446
LF+LK+ WR I +AD R+
Sbjct: 122 LFDLKVRWREILIADEVARRR 142
>SPBC36B7.09 |gcn2|ppk28, ppk28, SPBP18G5.01|eIF2 alpha kinase Gcn2
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1576
Score = 24.6 bits (51), Expect = 9.3
Identities = 15/47 (31%), Positives = 26/47 (55%), Gaps = 1/47 (2%)
Frame = -3
Query: 232 NQTLFQCNGVISYVFVYGFGYMKLI-DLFSESLFNILSQALFQFLPE 95
N LF +G ++ + FG+ + + D+ + FNI SQ++ LPE
Sbjct: 379 NVVLFH-SGHRTFAKLMDFGFTRTLRDMNASHPFNINSQSITNILPE 424
>SPAC4A8.05c |myp2|myo3|myosin II heavy chain |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 2104
Score = 24.6 bits (51), Expect = 9.3
Identities = 23/85 (27%), Positives = 42/85 (49%), Gaps = 3/85 (3%)
Frame = +2
Query: 17 SQQTKLFREKATMEGRLSELKTTLKTFRQELKKSLTKDI-EKTFREKIYE-LHVTKAID- 187
S +K R K + GRL E++ + F Q L ++ + ++K YE L+V K+ D
Sbjct: 1649 SSLSKAERSKNYLTGRLQEVEELARGF-QTTNADLQNELADAVVKQKEYEVLYVEKSNDY 1707
Query: 188 KDIRDYAVALEKCLIEFHREKMENI 262
+ L K + EFH +++++
Sbjct: 1708 NTLLLQKEKLMKQIDEFHVIRVQDL 1732
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,949,152
Number of Sequences: 5004
Number of extensions: 38367
Number of successful extensions: 164
Number of sequences better than 10.0: 19
Number of HSP's better than 10.0 without gapping: 157
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 162
length of database: 2,362,478
effective HSP length: 68
effective length of database: 2,022,206
effective search space used: 218398248
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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