Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= S06A01NCLL0002_J21
(563 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC4F6.12 |||LIM domain|Schizosaccharomyces pombe|chr 2|||Manual 82 5e-17
SPAC29A4.11 |rga3||GTPase activating protein Rga3|Schizosaccharo... 56 3e-09
SPBC3F6.05 |rga1||GTPase activating protein Rga1|Schizosaccharom... 55 8e-09
SPBC28E12.03 |rga4||GTPase activating protein Rga4|Schizosacchar... 43 3e-05
SPAPJ691.02 |||yippee-like protein|Schizosaccharomyces pombe|chr... 29 0.36
SPAC17A2.12 |||ATP-dependent DNA helicase|Schizosaccharomyces po... 27 1.9
SPCC4B3.18 |||phosphopantothenate-cysteine ligase|Schizosaccharo... 27 1.9
SPAC688.11 |end4|sla2|Huntingtin-interacting protein homolog|Sch... 26 3.3
SPBC4.06 |||acid phosphatase |Schizosaccharomyces pombe|chr 2|||... 26 4.4
SPBC106.12c |||THO complex subunit |Schizosaccharomyces pombe|ch... 25 5.8
SPAC18G6.09c |||sequence orphan|Schizosaccharomyces pombe|chr 1|... 25 7.7
SPBC17A3.04c |||methionine-tRNA ligase |Schizosaccharomyces pomb... 25 7.7
>SPBC4F6.12 |||LIM domain|Schizosaccharomyces pombe|chr 2|||Manual
Length = 438
Score = 82.2 bits (194), Expect = 5e-17
Identities = 45/159 (28%), Positives = 64/159 (40%), Gaps = 1/159 (0%)
Frame = +1
Query: 10 SYKDKHWHEACFLCAKCRVSLVDKQFGSKLDKIYCGNCYDAQFASRCDGCGEVFRAGTKK 189
S K H CF C C +L F + K YC Y QF+ RC C +
Sbjct: 272 SASGKKLHPQCFKCDTCSQNLEHVGFYYREGKFYCHLDYHEQFSPRCKHCKTPIE--DQA 329
Query: 190 MEYKTRQWHEKCFCCVVCKNPIGTK-SFIPREQEIYCAGCYEDKFATRCVKCNKIITQGG 366
+ +HE C C I R+ +C CY++K+A +C KC K I
Sbjct: 330 VHINNDWFHENHHFCAGCSEVFNVNIPCIYRDDLYWCQTCYDNKYAVKCKKCRKPILGIS 389
Query: 367 VTYKNEPWHRECFTCTHCNTSLAGQRFTSRDEKPYCAEC 483
V + +H +C+TC CN L + + + P C C
Sbjct: 390 VKGSDGEYHSQCWTCGACNALLGDEGYFMIENTPICRPC 428
Score = 74.9 bits (176), Expect = 7e-15
Identities = 40/140 (28%), Positives = 58/140 (41%), Gaps = 1/140 (0%)
Frame = +1
Query: 124 YDAQFASRCDGCGEVFRAGTKKMEYKTRQWHEKCFCCVVCKNPIGTKSFIPREQEIYCAG 303
Y C CG RAG + + ++ H +CF C C + F RE + YC
Sbjct: 250 YRGNSEKSCHSCGGSLRAG-RIISASGKKLHPQCFKCDTCSQNLEHVGFYYREGKFYCHL 308
Query: 304 CYEDKFATRCVKCNKIITQGGVTYKNEPWHRECFTCTHCNTSL-AGQRFTSRDEKPYCAE 480
Y ++F+ RC C I V N+ +H C C+ RD+ +C
Sbjct: 309 DYHEQFSPRCKHCKTPIEDQAVHINNDWFHENHHFCAGCSEVFNVNIPCIYRDDLYWCQT 368
Query: 481 CFGELFAKRCTSCTKPITGI 540
C+ +A +C C KPI GI
Sbjct: 369 CYDNKYAVKCKKCRKPILGI 388
>SPAC29A4.11 |rga3||GTPase activating protein
Rga3|Schizosaccharomyces pombe|chr 1|||Manual
Length = 969
Score = 56.4 bits (130), Expect = 3e-09
Identities = 27/115 (23%), Positives = 43/115 (37%), Gaps = 3/115 (2%)
Frame = +1
Query: 148 CDGCGEVFRAGTKKMEYKTRQWHEKCFCCVVCKNPIGTKSFI---PREQEIYCAGCYEDK 318
C CG+ F+ + + WH+ CFCC C + + + C+ C
Sbjct: 19 CFRCGQAFQRRETPISFGGHMWHKDCFCCTKCDKGLEHSDQMLVQTSDGRPVCSSC---- 74
Query: 319 FATRCVKCNKIITQGGVTYKNEPWHRECFTCTHCNTSLAGQRFTSRDEKPYCAEC 483
A C C I + + +HRECF C C + F + +C +C
Sbjct: 75 -AHTCTACRMRIKDYALMSGYDSYHRECFRCHDCRKQIIDSNFKRDNRTIFCNDC 128
Score = 41.1 bits (92), Expect = 1e-04
Identities = 28/110 (25%), Positives = 44/110 (40%), Gaps = 3/110 (2%)
Frame = +1
Query: 10 SYKDKHWHEACFLCAKCRVSLV--DKQFGSKLD-KIYCGNCYDAQFASRCDGCGEVFRAG 180
S+ WH+ CF C KC L D+ D + C +C A C C +
Sbjct: 34 SFGGHMWHKDCFCCTKCDKGLEHSDQMLVQTSDGRPVCSSC-----AHTCTACRMRIKDY 88
Query: 181 TKKMEYKTRQWHEKCFCCVVCKNPIGTKSFIPREQEIYCAGCYEDKFATR 330
Y + +H +CF C C+ I +F + I+C C + + +R
Sbjct: 89 ALMSGYDS--YHRECFRCHDCRKQIIDSNFKRDNRTIFCNDCKQVRHPSR 136
>SPBC3F6.05 |rga1||GTPase activating protein
Rga1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1150
Score = 54.8 bits (126), Expect = 8e-09
Identities = 36/134 (26%), Positives = 54/134 (40%), Gaps = 6/134 (4%)
Frame = +1
Query: 148 CDGCGEVFRAGTKKMEYKTRQWHEKCFCCVVCKNPIGTKSF-----IPREQEIYCAGCYE 312
C CG+V + + +H +CF C C + + +K F +Q C Y
Sbjct: 116 CASCGQVISG--QYVRALGNIYHLECFRCHDCNSLVASKFFPIDDPTLNKQVPLCETDYF 173
Query: 313 DKFATRCVKCNKIITQGGVTYKNEPWHRECFTCTHCNTSLA-GQRFTSRDEKPYCAECFG 489
+ C C + +T N+ +H E FTC+ C T + + K YC +
Sbjct: 174 RRLDLLCASCGMALRGYYITALNKKFHIEHFTCSLCYTVFGPNDSYYEYEGKVYCHYHYS 233
Query: 490 ELFAKRCTSCTKPI 531
LFA RC C PI
Sbjct: 234 TLFAARCCGCDGPI 247
Score = 37.1 bits (82), Expect = 0.002
Identities = 20/74 (27%), Positives = 30/74 (40%), Gaps = 5/74 (6%)
Frame = +1
Query: 331 CVKCNKIITQGGVTYKNEPWHRECFTCTHCNTSLAGQRF-----TSRDEKPYCAECFGEL 495
C C ++I+ V +H ECF C CN+ +A + F T + P C +
Sbjct: 116 CASCGQVISGQYVRALGNIYHLECFRCHDCNSLVASKFFPIDDPTLNKQVPLCETDYFRR 175
Query: 496 FAKRCTSCTKPITG 537
C SC + G
Sbjct: 176 LDLLCASCGMALRG 189
Score = 34.7 bits (76), Expect = 0.009
Identities = 22/72 (30%), Positives = 31/72 (43%), Gaps = 3/72 (4%)
Frame = +1
Query: 19 DKHWHEACFLCAKC-RVSLVDKQFGSKLDKIYCGNCYDAQFASRCDGC-GEVFRAGTKKM 192
+K +H F C+ C V + + K+YC Y FA+RC GC G + R +
Sbjct: 196 NKKFHIEHFTCSLCYTVFGPNDSYYEYEGKVYCHYHYSTLFAARCCGCDGPILRQFVEVY 255
Query: 193 EYKTRQ-WHEKC 225
Q WH C
Sbjct: 256 RNGVSQNWHVPC 267
>SPBC28E12.03 |rga4||GTPase activating protein
Rga4|Schizosaccharomyces pombe|chr 2|||Manual
Length = 933
Score = 43.2 bits (97), Expect = 3e-05
Identities = 21/70 (30%), Positives = 35/70 (50%), Gaps = 3/70 (4%)
Frame = +1
Query: 331 CVKC-NKIITQGGVTYKNEPWHRECFTCTHCNTSL--AGQRFTSRDEKPYCAECFGELFA 501
C+KC + + V + + WH +CF C +CN L + + F+ D+K + F +L
Sbjct: 24 CIKCWESVPSTSQVWFGGKCWHSDCFKCVNCNKKLDPSSEDFSQDDQK----QIFCKLCV 79
Query: 502 KRCTSCTKPI 531
C C+ PI
Sbjct: 80 DICNGCSTPI 89
Score = 35.1 bits (77), Expect = 0.007
Identities = 27/115 (23%), Positives = 47/115 (40%), Gaps = 3/115 (2%)
Frame = +1
Query: 148 CDGCGEVFRAGTKKMEYKTRQWHEKCFCCVVCKNPI--GTKSFIPREQ-EIYCAGCYEDK 318
C C E + T ++ + + WH CF CV C + ++ F +Q +I+C C +
Sbjct: 24 CIKCWESVPS-TSQVWFGGKCWHSDCFKCVNCNKKLDPSSEDFSQDDQKQIFCKLCVD-- 80
Query: 319 FATRCVKCNKIITQGGVTYKNEPWHRECFTCTHCNTSLAGQRFTSRDEKPYCAEC 483
C C+ I + ++ H C C++C + F + YC C
Sbjct: 81 ---ICNGCSTPICEFNAVSNSQANH-PC--CSNCRAFINSNAFGKFKGQHYCLPC 129
Score = 32.3 bits (70), Expect = 0.051
Identities = 27/99 (27%), Positives = 37/99 (37%), Gaps = 4/99 (4%)
Frame = +1
Query: 22 KHWHEACFLCAKCRVSL--VDKQFGSKLDK-IYCGNCYDAQFASRCDGCGEVFRAGTKKM 192
K WH CF C C L + F K I+C C D C+GC T
Sbjct: 42 KCWHSDCFKCVNCNKKLDPSSEDFSQDDQKQIFCKLCVDI-----CNGC------STPIC 90
Query: 193 EYKTRQWHEKCF-CCVVCKNPIGTKSFIPREQEIYCAGC 306
E+ + CC C+ I + +F + + YC C
Sbjct: 91 EFNAVSNSQANHPCCSNCRAFINSNAFGKFKGQHYCLPC 129
>SPAPJ691.02 |||yippee-like protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 131
Score = 29.5 bits (63), Expect = 0.36
Identities = 24/86 (27%), Positives = 32/86 (37%)
Frame = +1
Query: 7 GSYKDKHWHEACFLCAKCRVSLVDKQFGSKLDKIYCGNCYDAQFASRCDGCGEVFRAGTK 186
G Y H C++CAKC+ L K G L Y G A R + V K
Sbjct: 2 GRYYPVHLKSRCYVCAKCKTHLAFK--GHLLSHDYRGKNGPACLFKRVE---NVIEMEPK 56
Query: 187 KMEYKTRQWHEKCFCCVVCKNPIGTK 264
+ T ++ + C C IG K
Sbjct: 57 TEQMSTGRFIVRHIHCCRCHTYIGWK 82
>SPAC17A2.12 |||ATP-dependent DNA helicase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 897
Score = 27.1 bits (57), Expect = 1.9
Identities = 10/27 (37%), Positives = 14/27 (51%)
Frame = +1
Query: 403 FTCTHCNTSLAGQRFTSRDEKPYCAEC 483
FTC C + L GQ++ S A+C
Sbjct: 625 FTCQECMSMLVGQKYGSSSTSTIIAKC 651
>SPCC4B3.18 |||phosphopantothenate-cysteine
ligase|Schizosaccharomyces pombe|chr 3|||Manual
Length = 316
Score = 27.1 bits (57), Expect = 1.9
Identities = 14/37 (37%), Positives = 23/37 (62%)
Frame = -3
Query: 387 RLVLVRHTALSDYLVTLNAASGKLVFIAPGAVDLLFA 277
RL+++ +T L++YL L A + +L IA A+ L A
Sbjct: 143 RLIMIPYTTLTEYLWYLKAIAERLQIIASRALFYLAA 179
>SPAC688.11 |end4|sla2|Huntingtin-interacting protein
homolog|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1092
Score = 26.2 bits (55), Expect = 3.3
Identities = 18/50 (36%), Positives = 24/50 (48%), Gaps = 2/50 (4%)
Frame = -3
Query: 180 SSTEHLPTSI--ASAGELSIIAVAAVDLVQFGAKLLIHQGDAALGAEEAC 37
SS EHL + SA ++A + V F +K+ H DAA EAC
Sbjct: 976 SSFEHLIVACNGVSAATAQLVAASRVK-ANFASKVQDHLEDAAKAVTEAC 1024
>SPBC4.06 |||acid phosphatase |Schizosaccharomyces pombe|chr
2|||Manual
Length = 462
Score = 25.8 bits (54), Expect = 4.4
Identities = 19/59 (32%), Positives = 25/59 (42%)
Frame = -3
Query: 447 EPLSGERGVAVGASEALAVPRLVLVRHTALSDYLVTLNAASGKLVFIAPGAVDLLFARD 271
+PL R + + P V+ H LS L T+NAA G V P D + RD
Sbjct: 214 DPLLAGRASEMMSQFMNGQPVRVVSSHPRLSGLLDTINAAIGSHVDFNPNLRDEQWLRD 272
>SPBC106.12c |||THO complex subunit |Schizosaccharomyces pombe|chr
2|||Manual
Length = 274
Score = 25.4 bits (53), Expect = 5.8
Identities = 12/27 (44%), Positives = 13/27 (48%)
Frame = +3
Query: 375 QERAVAPRVLHLHPLQHLARRTAVHVE 455
QE+ PR HL QH R V VE
Sbjct: 64 QEQDAHPRTTHLQKRQHSENRFGVRVE 90
>SPAC18G6.09c |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 312
Score = 25.0 bits (52), Expect = 7.7
Identities = 13/33 (39%), Positives = 20/33 (60%)
Frame = -3
Query: 342 TLNAASGKLVFIAPGAVDLLFARDKALRTDRVL 244
TLN+ + + + A+DLLF RD+ R R+L
Sbjct: 275 TLNSPNNGIHCHSTSALDLLFHRDREQRLFRML 307
>SPBC17A3.04c |||methionine-tRNA ligase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 782
Score = 25.0 bits (52), Expect = 7.7
Identities = 10/23 (43%), Positives = 12/23 (52%), Gaps = 1/23 (4%)
Frame = +1
Query: 112 CGNC-YDAQFASRCDGCGEVFRA 177
C C YD +CDGCG + A
Sbjct: 363 CPKCGYDDARGDQCDGCGGLLNA 385
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,261,427
Number of Sequences: 5004
Number of extensions: 46502
Number of successful extensions: 175
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 151
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 166
length of database: 2,362,478
effective HSP length: 69
effective length of database: 2,017,202
effective search space used: 238029836
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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