Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= S06A01NCLL0002_J13
(511 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC11E3.04c |ubc13|spu13|ubiquitin conjugating enzyme Ubc13|Sch... 62 4e-11
SPBC2D10.20 |ubc1||ubiquitin conjugating enzyme Ubc1|Schizosacch... 57 1e-09
SPAC18B11.07c |rhp6|ubc2|Rad6 homolog, ubiquitin conjugating enz... 54 9e-09
SPBC1198.09 |ubc16||ubiquitin conjugating enzyme Ubc16|Schizosac... 54 9e-09
SPBC119.02 |ubc4||ubiquitin conjugating enzyme Ubc4|Schizosaccha... 54 1e-08
SPBC211.07c |ubc8||ubiquitin conjugating enzyme Ubc8|Schizosacch... 53 3e-08
SPBC1105.09 |ubc15||ubiquitin conjugating enzyme Ubc15|Schizosac... 47 2e-06
SPCC777.10c |ubc12||ubiquitin conjugating enzyme Ubc12|Schizosac... 44 2e-05
SPAC1250.03 |ubc14||ubiquitin conjugating enzyme Ubc14|Schizosac... 43 2e-05
SPBP16F5.04 |ubc3|ubcp3|ubiquitin conjugating enzyme Ubc3|Schizo... 42 4e-05
SPCC1259.15c |ubc11|ubcdp, ubcp4|ubiquitin conjugating enzyme E2... 40 2e-04
SPAC10F6.05c |ubc6||ubiquitin conjugating enzyme Ubc6|Schizosacc... 31 0.10
SPBC12D12.01 |sad1|SPBC16H5.01c|spindle pole body protein Sad1|S... 28 0.71
SPAC4A8.16c |tif33|SPAC823.01c|translation initiation factor eIF... 27 2.2
SPBC27B12.11c |||transcription factor |Schizosaccharomyces pombe... 27 2.2
SPBC365.02c |cox10||protoheme IX farnesyltransferase|Schizosacch... 27 2.2
SPBC1539.10 |||ribosome biogenesis protein Nop16 |Schizosaccharo... 26 2.9
SPBC14F5.07 |||ER-localized ubiquitin ligase |Schizosaccharomyce... 26 3.8
SPBC146.09c |lsd1|swm1, saf110|histone demethylase SWIRM1|Schizo... 25 5.0
>SPAC11E3.04c |ubc13|spu13|ubiquitin conjugating enzyme
Ubc13|Schizosaccharomyces pombe|chr 1|||Manual
Length = 148
Score = 62.5 bits (145), Expect = 4e-11
Identities = 30/92 (32%), Positives = 47/92 (51%)
Frame = +2
Query: 233 VARELRRLALHPPPGVKLQLRDDDFTDVLALIDGPAETPYAGGTFRVRLVLGREFPASAP 412
+ +E+ L PPPG+ +D+ ++GP ++ Y GG F + L L E+P P
Sbjct: 7 IIKEIETLTRDPPPGIVAAPTEDNLRYFKITMEGPQQSAYEGGKFHLELFLPDEYPMMPP 66
Query: 413 RAYFLNAPCSTPDVSPDTGEVCVNTLKRDWRP 508
FL P+V G +C++TLK+DW P
Sbjct: 67 NVRFL-TKIYHPNVD-KLGRICLSTLKKDWSP 96
>SPBC2D10.20 |ubc1||ubiquitin conjugating enzyme
Ubc1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 217
Score = 57.2 bits (132), Expect = 1e-09
Identities = 28/92 (30%), Positives = 48/92 (52%)
Frame = +2
Query: 233 VARELRRLALHPPPGVKLQLRDDDFTDVLALIDGPAETPYAGGTFRVRLVLGREFPASAP 412
+A+EL + G+++ +DD + + + GP TPY GG F V + + ++P P
Sbjct: 9 IAKELADVQQDKQAGIQVWTINDDISHLKGMFRGPEGTPYEGGYFVVDIEIPIDYPFRPP 68
Query: 413 RAYFLNAPCSTPDVSPDTGEVCVNTLKRDWRP 508
+ F + P+VS TG +C++ LK W P
Sbjct: 69 KMNF-DTKIYHPNVSSQTGAICLDILKDQWSP 99
>SPAC18B11.07c |rhp6|ubc2|Rad6 homolog, ubiquitin conjugating enzyme
E2 Rhp6|Schizosaccharomyces pombe|chr 1|||Manual
Length = 151
Score = 54.4 bits (125), Expect = 9e-09
Identities = 30/96 (31%), Positives = 50/96 (52%)
Frame = +2
Query: 221 ALNSVARELRRLALHPPPGVKLQLRDDDFTDVLALIDGPAETPYAGGTFRVRLVLGREFP 400
A + R+ +R+ PP GV D+ A+I GPA+TP+ GTF++ L ++P
Sbjct: 5 ARRRLMRDFKRMQQDPPAGVSASPVSDNVMLWNAVIIGPADTPFEDGTFKLVLSFDEQYP 64
Query: 401 ASAPRAYFLNAPCSTPDVSPDTGEVCVNTLKRDWRP 508
P F++ P+V + GE+C++ L+ W P
Sbjct: 65 NKPPLVKFVST-MFHPNVYAN-GELCLDILQNRWSP 98
>SPBC1198.09 |ubc16||ubiquitin conjugating enzyme
Ubc16|Schizosaccharomyces pombe|chr 2|||Manual
Length = 160
Score = 54.4 bits (125), Expect = 9e-09
Identities = 27/71 (38%), Positives = 38/71 (53%)
Frame = +2
Query: 296 DDDFTDVLALIDGPAETPYAGGTFRVRLVLGREFPASAPRAYFLNAPCSTPDVSPDTGEV 475
D D A+I+GP ETPY GG + + + + +P S P YF P++S GEV
Sbjct: 31 DVDMFHWKAVIEGPTETPYEGGQWVLDIHVHEGYPISPPSVYF-QTKIVHPNISWTNGEV 89
Query: 476 CVNTLKRDWRP 508
C++ LK W P
Sbjct: 90 CMDILKTHWSP 100
>SPBC119.02 |ubc4||ubiquitin conjugating enzyme
Ubc4|Schizosaccharomyces pombe|chr 2|||Manual
Length = 147
Score = 54.0 bits (124), Expect = 1e-08
Identities = 30/96 (31%), Positives = 45/96 (46%)
Frame = +2
Query: 221 ALNSVARELRRLALHPPPGVKLQLRDDDFTDVLALIDGPAETPYAGGTFRVRLVLGREFP 400
AL + REL L PP DD A I GPA++PYAGG F + + ++P
Sbjct: 2 ALKRINRELADLGKDPPSSCSAGPVGDDLFHWQATIMGPADSPYAGGVFFLSIHFPTDYP 61
Query: 401 ASAPRAYFLNAPCSTPDVSPDTGEVCVNTLKRDWRP 508
P+ F P+++ G +C++ L+ W P
Sbjct: 62 FKPPKVNF-TTRIYHPNIN-SNGSICLDILRDQWSP 95
>SPBC211.07c |ubc8||ubiquitin conjugating enzyme
Ubc8|Schizosaccharomyces pombe|chr 2|||Manual
Length = 184
Score = 52.8 bits (121), Expect = 3e-08
Identities = 23/76 (30%), Positives = 43/76 (56%)
Frame = +2
Query: 281 KLQLRDDDFTDVLALIDGPAETPYAGGTFRVRLVLGREFPASAPRAYFLNAPCSTPDVSP 460
++ L +D+ + GP+ETPY+GG ++V + L E+P +P F+N P++
Sbjct: 21 EVTLVNDNMQEFYVRFHGPSETPYSGGIWKVHVELPSEYPWKSPSIGFVNR-IFHPNIDE 79
Query: 461 DTGEVCVNTLKRDWRP 508
+G VC++ + + W P
Sbjct: 80 LSGSVCLDVINQTWSP 95
>SPBC1105.09 |ubc15||ubiquitin conjugating enzyme
Ubc15|Schizosaccharomyces pombe|chr 2|||Manual
Length = 167
Score = 46.8 bits (106), Expect = 2e-06
Identities = 27/85 (31%), Positives = 44/85 (51%), Gaps = 1/85 (1%)
Frame = +2
Query: 239 RELRRLALHPPPGVKLQLRDD-DFTDVLALIDGPAETPYAGGTFRVRLVLGREFPASAPR 415
++L+ + +PP G + L DD + +I GP +T Y GG F L +++P P+
Sbjct: 12 KQLKEIQKNPPQGFSVGLVDDKSIFEWEVMIIGPEDTLYEGGFFHATLSFPQDYPLMPPK 71
Query: 416 AYFLNAPCSTPDVSPDTGEVCVNTL 490
F P+V P+ GEVC++ L
Sbjct: 72 MKF-TTEIWHPNVHPN-GEVCISIL 94
>SPCC777.10c |ubc12||ubiquitin conjugating enzyme
Ubc12|Schizosaccharomyces pombe|chr 3|||Manual
Length = 177
Score = 43.6 bits (98), Expect = 2e-05
Identities = 29/104 (27%), Positives = 49/104 (47%)
Frame = +2
Query: 197 NAENVCPQALNSVARELRRLALHPPPGVKLQLRDDDFTDVLALIDGPAETPYAGGTFRVR 376
NA + P + + +++ L + P + D +VL L P E Y GG F+ R
Sbjct: 17 NASGISPAQIR-IQKDVTDLEI--PSTMSTSWPDPIKLNVLHLEIRPDEGYYKGGKFKFR 73
Query: 377 LVLGREFPASAPRAYFLNAPCSTPDVSPDTGEVCVNTLKRDWRP 508
+ + +P P+ LN P++ + G VC+N L++DW P
Sbjct: 74 IQIDDNYPHDPPKVKCLNK-IYHPNIDIE-GNVCLNILRQDWNP 115
>SPAC1250.03 |ubc14||ubiquitin conjugating enzyme
Ubc14|Schizosaccharomyces pombe|chr 1|||Manual
Length = 155
Score = 43.2 bits (97), Expect = 2e-05
Identities = 26/94 (27%), Positives = 42/94 (44%)
Frame = +2
Query: 215 PQALNSVARELRRLALHPPPGVKLQLRDDDFTDVLALIDGPAETPYAGGTFRVRLVLGRE 394
P + + +E L HP P +++ L DD+ GP+++ YAGG F L +
Sbjct: 6 PSSSRRLTKEYSDLREHPIPDIRVNLVDDNLFHWACTALGPSDSVYAGGKFHFSLKFPLD 65
Query: 395 FPASAPRAYFLNAPCSTPDVSPDTGEVCVNTLKR 496
+P P F P+ + G VC+ LK+
Sbjct: 66 YPFQPPTIEF-TTRIYHPNFDSE-GNVCLAILKQ 97
>SPBP16F5.04 |ubc3|ubcp3|ubiquitin conjugating enzyme
Ubc3|Schizosaccharomyces pombe|chr 2|||Manual
Length = 166
Score = 42.3 bits (95), Expect = 4e-05
Identities = 28/91 (30%), Positives = 41/91 (45%), Gaps = 1/91 (1%)
Frame = +2
Query: 221 ALNSVARELRRLALHPPPGVKL-QLRDDDFTDVLALIDGPAETPYAGGTFRVRLVLGREF 397
AL + +E + L + P G+ +DDF LI GP TP+ GG + L ++
Sbjct: 6 ALRRLMKEYKELTENGPDGITAGPSNEDDFFTWDCLIQGPDGTPFEGGLYPATLKFPSDY 65
Query: 398 PASAPRAYFLNAPCSTPDVSPDTGEVCVNTL 490
P P F P+V D G VC++ L
Sbjct: 66 PLGPPTLKF-ECEFFHPNVYKD-GTVCISIL 94
>SPCC1259.15c |ubc11|ubcdp, ubcp4|ubiquitin conjugating enzyme E2-C
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 176
Score = 40.3 bits (90), Expect = 2e-04
Identities = 25/88 (28%), Positives = 40/88 (45%), Gaps = 1/88 (1%)
Frame = +2
Query: 242 ELRRLALHPPPGVK-LQLRDDDFTDVLALIDGPAETPYAGGTFRVRLVLGREFPASAPRA 418
EL L + PG+ D + I GP++T Y G F++ + +P S P
Sbjct: 36 ELMSLMMSNTPGISAFPDSDSNLLHWAGTITGPSDTYYEGLKFKISMSFPANYPYSPPTI 95
Query: 419 YFLNAPCSTPDVSPDTGEVCVNTLKRDW 502
F +P P+V +G +C++ LK W
Sbjct: 96 IF-TSPMWHPNVDM-SGNICLDILKDKW 121
>SPAC10F6.05c |ubc6||ubiquitin conjugating enzyme
Ubc6|Schizosaccharomyces pombe|chr 1|||Manual
Length = 227
Score = 31.1 bits (67), Expect = 0.10
Identities = 16/64 (25%), Positives = 29/64 (45%)
Frame = +2
Query: 221 ALNSVARELRRLALHPPPGVKLQLRDDDFTDVLALIDGPAETPYAGGTFRVRLVLGREFP 400
A + +E L +P V + ++ + +I GP +TPY GG + L+ ++P
Sbjct: 6 AYKRLMKEYLALQKNPVELVDAKPATENILEWHYIITGPPDTPYEGGQYHGTLIFPPDYP 65
Query: 401 ASAP 412
P
Sbjct: 66 FKPP 69
>SPBC12D12.01 |sad1|SPBC16H5.01c|spindle pole body protein
Sad1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 514
Score = 28.3 bits (60), Expect = 0.71
Identities = 11/23 (47%), Positives = 15/23 (65%)
Frame = -2
Query: 420 YARGADAGNSRPSTSRTRNVPPA 352
Y+R + NS+PS S N+PPA
Sbjct: 195 YSRSFSSDNSQPSNSPVPNIPPA 217
>SPAC4A8.16c |tif33|SPAC823.01c|translation initiation factor
eIF3c|Schizosaccharomyces pombe|chr 1|||Manual
Length = 918
Score = 26.6 bits (56), Expect = 2.2
Identities = 12/34 (35%), Positives = 20/34 (58%)
Frame = +1
Query: 91 YIYIILNKGLIDN*KEALQDASEGVSPGQWRMCD 192
YI L G +N +E + AS+ ++ G+WR C+
Sbjct: 731 YIDRQLFVGPPENTREYIMQASKALADGEWRRCE 764
>SPBC27B12.11c |||transcription factor |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 738
Score = 26.6 bits (56), Expect = 2.2
Identities = 13/31 (41%), Positives = 16/31 (51%)
Frame = -1
Query: 253 ATQFSRHAVQRLGAHILRVRHRTSSTGLDSH 161
AT S+H V+R H LR H GL+ H
Sbjct: 643 ATDSSQHVVERPCIHSLRGIHGQQDGGLEQH 673
>SPBC365.02c |cox10||protoheme IX
farnesyltransferase|Schizosaccharomyces pombe|chr
2|||Manual
Length = 387
Score = 26.6 bits (56), Expect = 2.2
Identities = 16/53 (30%), Positives = 26/53 (49%), Gaps = 2/53 (3%)
Frame = +2
Query: 215 PQALNSVARELRRLALHPPPGVKLQLRDDDFT--DVLALIDGPAETPYAGGTF 367
PQ L +++ ++ L+P P L+L T VL+ + A PY G +F
Sbjct: 65 PQVLTFLSKRMQAAPLYPKPSAFLELGKPRLTVLVVLSTMSSYALAPYPGLSF 117
>SPBC1539.10 |||ribosome biogenesis protein Nop16
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 209
Score = 26.2 bits (55), Expect = 2.9
Identities = 14/37 (37%), Positives = 21/37 (56%)
Frame = +2
Query: 236 ARELRRLALHPPPGVKLQLRDDDFTDVLALIDGPAET 346
+ EL L PPG + RDDD +++ +I G A+T
Sbjct: 84 SEELDELKKSLPPGQAIVRRDDD-GNIIEIIHGEAKT 119
>SPBC14F5.07 |||ER-localized ubiquitin ligase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 1242
Score = 25.8 bits (54), Expect = 3.8
Identities = 13/35 (37%), Positives = 17/35 (48%)
Frame = +1
Query: 379 SAGTRIPRVCAARVFPKRAVFHPRRITGHGRGVRE 483
+A I RVC P +FHP + TG R V +
Sbjct: 2 NADDEICRVCRCEGAPDSPLFHPCKCTGSIRYVHQ 36
>SPBC146.09c |lsd1|swm1, saf110|histone demethylase
SWIRM1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1000
Score = 25.4 bits (53), Expect = 5.0
Identities = 17/54 (31%), Positives = 26/54 (48%), Gaps = 3/54 (5%)
Frame = +2
Query: 341 ETPYAGGTFRVRLVLGREFPASAPR---AYFLNAPCSTPDVSPDTGEVCVNTLK 493
E Y G + L+LG + + + A LN+ STP P + CV+T+K
Sbjct: 492 ENVYPGSIPNLNLLLGENVASYSFKHGMADMLNSLASTPSPLPILFDQCVHTVK 545
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,035,910
Number of Sequences: 5004
Number of extensions: 38629
Number of successful extensions: 134
Number of sequences better than 10.0: 19
Number of HSP's better than 10.0 without gapping: 126
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 129
length of database: 2,362,478
effective HSP length: 68
effective length of database: 2,022,206
effective search space used: 204242806
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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