BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= S06A01NCLL0002_J10 (434 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC11E3.15 |rpl22|SPAP8A3.01|60S ribosomal protein L22|Schizosa... 84 1e-17 SPBC8D2.06 |||isoleucine-tRNA ligase |Schizosaccharomyces pombe|... 29 0.41 SPAC1834.02 |aro1||pentafunctional aromatic polypeptide Aro1 |Sc... 25 3.8 SPAC1486.05 |nup189||nucleoporin Nup189|Schizosaccharomyces pomb... 25 6.7 SPCC622.10c |||exocyst complex subunit Sec5 |Schizosaccharomyces... 24 8.8 SPBC543.08 |||phosphoinositide biosynthesis protein |Schizosacch... 24 8.8 SPBC14F5.10c |||ubiquitin-protein ligase E3 |Schizosaccharomyces... 24 8.8 >SPAC11E3.15 |rpl22|SPAP8A3.01|60S ribosomal protein L22|Schizosaccharomyces pombe|chr 1|||Manual Length = 117 Score = 83.8 bits (198), Expect = 1e-17 Identities = 43/98 (43%), Positives = 57/98 (58%), Gaps = 1/98 (1%) Frame = +3 Query: 144 ISLKFTIDCTHPAEDSILDVGNFEKYLKERVKVEGKTNNLGNHVVIARD-KTKVAINADI 320 +S K+ ID T D I DV FEKYL +R+KV+GKT NLG+ VV++R+ +K+A+ A I Sbjct: 8 VSNKYIIDATAAVNDKIFDVAAFEKYLIDRIKVDGKTGNLGSSVVVSREGSSKIAVIAHI 67 Query: 321 PFSXXXXXXXXXXXXXXXXXXDWLRVVASAHDSYELRY 434 FS DWLRVV++ YELRY Sbjct: 68 DFSGRYLKYLTKKFLKKHSLRDWLRVVSTKKGVYELRY 105 >SPBC8D2.06 |||isoleucine-tRNA ligase |Schizosaccharomyces pombe|chr 2|||Manual Length = 1064 Score = 28.7 bits (61), Expect = 0.41 Identities = 19/62 (30%), Positives = 35/62 (56%), Gaps = 1/62 (1%) Frame = -2 Query: 274 T*LPRLFVLPSTLTRSFKYFSKLPTSRMLSSAGCVQSMVNLRLIF-LLAPLPRILPPFTP 98 T +P+L L +T + F++ R+ G ++++ L ++F +L L RI+ PFTP Sbjct: 714 TVVPQLLGLIEEMTNWYIRFNR---RRLKGEDGEIETINALNVLFEVLFTLVRIMGPFTP 770 Query: 97 FL 92 F+ Sbjct: 771 FI 772 >SPAC1834.02 |aro1||pentafunctional aromatic polypeptide Aro1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1573 Score = 25.4 bits (53), Expect = 3.8 Identities = 9/30 (30%), Positives = 19/30 (63%) Frame = +3 Query: 234 VKVEGKTNNLGNHVVIARDKTKVAINADIP 323 +K+ G NNL +++ + +T++ + DIP Sbjct: 1210 IKLVGMANNLNDNLELEEFRTRITNSMDIP 1239 >SPAC1486.05 |nup189||nucleoporin Nup189|Schizosaccharomyces pombe|chr 1|||Manual Length = 1778 Score = 24.6 bits (51), Expect = 6.7 Identities = 9/25 (36%), Positives = 15/25 (60%) Frame = +3 Query: 198 DVGNFEKYLKERVKVEGKTNNLGNH 272 + GN +KY + +KV GK ++ H Sbjct: 955 EAGNLKKYDQPNLKVSGKNDSFVTH 979 >SPCC622.10c |||exocyst complex subunit Sec5 |Schizosaccharomyces pombe|chr 3|||Manual Length = 815 Score = 24.2 bits (50), Expect = 8.8 Identities = 8/22 (36%), Positives = 14/22 (63%) Frame = +1 Query: 10 SATRVLLPRWQLQRSPSQRKAS 75 +ATR ++ W LQ +P + A+ Sbjct: 500 AATRCIVDTWMLQHAPQSKSAN 521 >SPBC543.08 |||phosphoinositide biosynthesis protein |Schizosaccharomyces pombe|chr 2|||Manual Length = 250 Score = 24.2 bits (50), Expect = 8.8 Identities = 12/28 (42%), Positives = 15/28 (53%), Gaps = 1/28 (3%) Frame = +1 Query: 337 ISNT*PSVTSRKTIFGT-GSEWWLLHMT 417 I N TS K +FG G WW+L +T Sbjct: 180 ILNNGIKATSTKVLFGLLGLWWWMLFVT 207 >SPBC14F5.10c |||ubiquitin-protein ligase E3 |Schizosaccharomyces pombe|chr 2|||Manual Length = 486 Score = 24.2 bits (50), Expect = 8.8 Identities = 7/21 (33%), Positives = 13/21 (61%) Frame = +2 Query: 68 RPVTPEDW*ERSKRWQNSRQR 130 R P++W ER K W+ +++ Sbjct: 229 RDFYPDNWLERQKSWEEEKEQ 249 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 1,724,015 Number of Sequences: 5004 Number of extensions: 31588 Number of successful extensions: 85 Number of sequences better than 10.0: 7 Number of HSP's better than 10.0 without gapping: 83 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 84 length of database: 2,362,478 effective HSP length: 67 effective length of database: 2,027,210 effective search space used: 156095170 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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