Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= S06A01NCLL0002_I03
(409 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At4g34555.1 68417.m04910 40S ribosomal protein S25, putative 93 7e-20
At4g39200.1 68417.m05550 40S ribosomal protein S25 (RPS25E) ribo... 91 2e-19
At2g21580.1 68415.m02567 40S ribosomal protein S25 (RPS25B) 91 2e-19
At2g16360.1 68415.m01872 40S ribosomal protein S25 (RPS25A) 85 2e-17
At5g20320.1 68418.m02418 DEAD/DEAH box helicase, putative simila... 31 0.22
At1g69430.1 68414.m07978 hypothetical protein 28 2.1
At3g09040.1 68416.m01063 pentatricopeptide (PPR) repeat-containi... 28 2.8
At2g17250.1 68415.m01992 expressed protein weak similarity to Ri... 28 2.8
At1g48180.1 68414.m05378 expressed protein ; expression supporte... 27 3.7
At2g22180.1 68415.m02634 hydroxyproline-rich glycoprotein family... 27 4.8
At1g24706.1 68414.m03104 expressed protein 27 4.8
At5g50140.1 68418.m06210 ankyrin repeat family protein contains ... 26 8.5
At5g37570.1 68418.m04526 pentatricopeptide (PPR) repeat-containi... 26 8.5
>At4g34555.1 68417.m04910 40S ribosomal protein S25, putative
Length = 108
Score = 93.1 bits (221), Expect = 7e-20
Identities = 43/72 (59%), Positives = 55/72 (76%)
Frame = +3
Query: 120 LNNQVLFDKPTYEKLYKEVPQYKLITPAVVSERLKIRGSLARRALVELRDKGLIKQVVQH 299
+NN VLFD+ TY+KL E P++KLITP+++S+RL+I GSLARRA+ EL KG I+ V H
Sbjct: 37 VNNMVLFDQATYDKLLSEAPKFKLITPSILSDRLRINGSLARRAIRELMAKGTIRMVSAH 96
Query: 300 HGQVIYTRATKG 335
Q IYTRAT G
Sbjct: 97 SSQQIYTRATHG 108
>At4g39200.1 68417.m05550 40S ribosomal protein S25 (RPS25E)
ribosomal protein S25, Lycopersicon esculentum,
PIR2:S40089
Length = 108
Score = 91.5 bits (217), Expect = 2e-19
Identities = 41/70 (58%), Positives = 55/70 (78%)
Frame = +3
Query: 120 LNNQVLFDKPTYEKLYKEVPQYKLITPAVVSERLKIRGSLARRALVELRDKGLIKQVVQH 299
+NN VLFD+ TY+KL E P++KLITP+++S+R++I GSLARRA+ EL KG+I+ V H
Sbjct: 37 VNNMVLFDQATYDKLLTEAPKFKLITPSILSDRMRINGSLARRAIRELMAKGVIRMVAAH 96
Query: 300 HGQVIYTRAT 329
Q IYTRAT
Sbjct: 97 SSQQIYTRAT 106
>At2g21580.1 68415.m02567 40S ribosomal protein S25 (RPS25B)
Length = 108
Score = 91.5 bits (217), Expect = 2e-19
Identities = 42/70 (60%), Positives = 55/70 (78%)
Frame = +3
Query: 120 LNNQVLFDKPTYEKLYKEVPQYKLITPAVVSERLKIRGSLARRALVELRDKGLIKQVVQH 299
+NN VLFD+ TY+KL E P++KLITP+++S+R++I GSLARRA+ EL KGLI+ V H
Sbjct: 37 VNNMVLFDQGTYDKLLTEAPKFKLITPSILSDRMRINGSLARRAIRELMAKGLIRMVSAH 96
Query: 300 HGQVIYTRAT 329
Q IYTRAT
Sbjct: 97 SSQQIYTRAT 106
>At2g16360.1 68415.m01872 40S ribosomal protein S25 (RPS25A)
Length = 125
Score = 84.6 bits (200), Expect = 2e-17
Identities = 39/70 (55%), Positives = 53/70 (75%)
Frame = +3
Query: 120 LNNQVLFDKPTYEKLYKEVPQYKLITPAVVSERLKIRGSLARRALVELRDKGLIKQVVQH 299
+NN VLFD+ TY+KL E P++KLITP+++S+RL+I GSLAR+A+ +L KG I+ V H
Sbjct: 53 VNNMVLFDQATYDKLMSEAPKFKLITPSILSDRLRINGSLARKAIRDLMVKGTIRMVSTH 112
Query: 300 HGQVIYTRAT 329
Q I TRAT
Sbjct: 113 SSQQINTRAT 122
>At5g20320.1 68418.m02418 DEAD/DEAH box helicase, putative similar to
CAF protein [Arabidopsis thaliana] GI:6102610; contains
Pfam profiles PF00270: DEAD/DEAH box helicase, PF00271:
Helicase conserved C-terminal domain, PF03368: Domain of
unknown function, PF00636: RNase3 domain, PF00035:
Double-stranded RNA binding motif
Length = 1676
Score = 31.5 bits (68), Expect = 0.22
Identities = 21/66 (31%), Positives = 33/66 (50%), Gaps = 1/66 (1%)
Frame = -3
Query: 299 VLDYLFD*TFISKFDKCSPGKGASDLQPFR-DNSGCDQLVLWYFLIQFLICGFVKQHLVV 123
VLDYL F + F K PG+ +DL+ +N + + + L +FL C + H V+
Sbjct: 1310 VLDYLMTSYFFTVFPKLKPGQ-LTDLRSLSVNNEALANVAVSFSLKRFLFCESIYLHEVI 1368
Query: 122 *LVTDF 105
T+F
Sbjct: 1369 EDYTNF 1374
>At1g69430.1 68414.m07978 hypothetical protein
Length = 350
Score = 28.3 bits (60), Expect = 2.1
Identities = 9/28 (32%), Positives = 18/28 (64%)
Frame = -2
Query: 162 VSHMWVCQTTLGCLTCHGLFLLTTSSSW 79
++++W+C + CLT +FL+ SS+
Sbjct: 172 ITYLWICTVIVVCLTSFCVFLVAVCSSF 199
>At3g09040.1 68416.m01063 pentatricopeptide (PPR) repeat-containing
protein contains INTERPRO:IPR002885 PPR repeats
Length = 1028
Score = 27.9 bits (59), Expect = 2.8
Identities = 22/66 (33%), Positives = 29/66 (43%)
Frame = -3
Query: 362 SIILSNRVISLSRSCVDHLPMVLDYLFD*TFISKFDKCSPGKGASDLQPFRDNSGCDQLV 183
S++ S R S + HL LD L T I + KC KG+S Q F + +V
Sbjct: 743 SVLSSLREGRAIHSLIFHLAHDLDELTSNTLIDMYAKCGDMKGSS--QVFDEMRRRSNVV 800
Query: 182 LWYFLI 165
W LI
Sbjct: 801 SWNSLI 806
>At2g17250.1 68415.m01992 expressed protein weak similarity to
Ribosome biogenesis protein MAK21 (Swiss-Prot:Q12176)
[Saccharomyces cerevisiae]
Length = 577
Score = 27.9 bits (59), Expect = 2.8
Identities = 11/36 (30%), Positives = 24/36 (66%)
Frame = +3
Query: 123 NNQVLFDKPTYEKLYKEVPQYKLITPAVVSERLKIR 230
+++ + +KPT +K E L++PA +S+R+K++
Sbjct: 245 SDESISEKPTDKKKKTEKGDSTLLSPATISKRMKLK 280
>At1g48180.1 68414.m05378 expressed protein ; expression supported
by MPSS
Length = 239
Score = 27.5 bits (58), Expect = 3.7
Identities = 13/31 (41%), Positives = 18/31 (58%)
Frame = -1
Query: 235 EPRIFNLSETTAGVISLYCGTSLYSFSYVGL 143
+P I L + + + +YCGTS SYVGL
Sbjct: 144 KPHI-ELQQDASAITGIYCGTSGEPASYVGL 173
>At2g22180.1 68415.m02634 hydroxyproline-rich glycoprotein family
protein
Length = 291
Score = 27.1 bits (57), Expect = 4.8
Identities = 13/36 (36%), Positives = 23/36 (63%)
Frame = -3
Query: 200 GCDQLVLWYFLIQFLICGFVKQHLVV*LVTDFSF*P 93
GC + +LW+F+I +I GF+ ++ L+ F+F P
Sbjct: 115 GCSKRLLWFFII-LVIFGFLLGAII--LILHFAFNP 147
>At1g24706.1 68414.m03104 expressed protein
Length = 1781
Score = 27.1 bits (57), Expect = 4.8
Identities = 10/21 (47%), Positives = 13/21 (61%)
Frame = -1
Query: 121 NLSRTFPFDHFFFLALPPPDP 59
+L ++ P DHF LPPP P
Sbjct: 1562 SLEKSHPDDHFHSQGLPPPPP 1582
>At5g50140.1 68418.m06210 ankyrin repeat family protein contains
ankyrin repeat domains, Pfam:PF00023
Length = 535
Score = 26.2 bits (55), Expect = 8.5
Identities = 10/31 (32%), Positives = 21/31 (67%)
Frame = +3
Query: 255 VELRDKGLIKQVVQHHGQVIYTRATKGDDPV 347
V+L ++ L+K++V+ H ++ + TK D P+
Sbjct: 35 VKLGNEELVKKIVEIHPSLVSSTNTKSDTPL 65
>At5g37570.1 68418.m04526 pentatricopeptide (PPR) repeat-containing
protein contains Pfam profile PF01535: PPR repeat
Length = 550
Score = 26.2 bits (55), Expect = 8.5
Identities = 15/47 (31%), Positives = 27/47 (57%), Gaps = 2/47 (4%)
Frame = +3
Query: 159 KLYKEVPQYKLITPAVVSERLKIR--GSLARRALVELRDKGLIKQVV 293
KL++E+PQ L++ + E + I GS A R ++ D+G++ V
Sbjct: 361 KLFEEMPQRDLVSYCSMMEGMAIHGCGSEAIRLFEKMVDEGIVPDEV 407
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 7,364,768
Number of Sequences: 28952
Number of extensions: 130821
Number of successful extensions: 364
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 361
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 364
length of database: 12,070,560
effective HSP length: 74
effective length of database: 9,928,112
effective search space used: 605614832
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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