Samia BLAST Result against arabidopsis

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= S06A01NCLL0002_H08
         (514 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At5g02490.1 68418.m00182 heat shock cognate 70 kDa protein 2 (HS...    28   3.2  
At4g16340.1 68417.m02476 adapter protein SPIKE1 (SPK1) One model...    28   4.2  
At5g02500.1 68418.m00183 heat shock cognate 70 kDa protein 1 (HS...    27   7.4  
At2g39930.1 68415.m04907 isoamylase, putative / starch debranchi...    27   7.4  

>At5g02490.1 68418.m00182 heat shock cognate 70 kDa protein 2
           (HSC70-2) (HSP70-2) identical to SP|P22954 Heat shock
           cognate 70 kDa protein 2 (Hsc70.2) {Arabidopsis
           thaliana}
          Length = 653

 Score = 28.3 bits (60), Expect = 3.2
 Identities = 13/42 (30%), Positives = 23/42 (54%)
 Frame = +1

Query: 307 ILGTLVIAVTSLPISEDKFFIDYSDGVIGEFGEHFEGDMVLT 432
           ++ TL+   T++P  +++ F  YSD   G   + FEG+   T
Sbjct: 415 VMTTLIQRNTTIPTKKEQVFSTYSDNQPGVLIQVFEGERART 456


>At4g16340.1 68417.m02476 adapter protein SPIKE1 (SPK1) One model
           reflects the alignment of a full-length cDNA sequence
           gi:18496702.  There are multiple frame shifts in the
           gene model resulting in a truncated protein.  The
           alternate model includes modifications in exons 14, 17
           and 29 to compensate for frame shifts and maximize the
           protein length.  It is not based on EST data.  adapter
           protein SPIKE1 [Arabidopsis thaliana] GI:18496703
          Length = 1757

 Score = 27.9 bits (59), Expect = 4.2
 Identities = 14/37 (37%), Positives = 21/37 (56%), Gaps = 1/37 (2%)
 Frame = -3

Query: 485 LSPFRASIA-AVIACFCLEVNTISPSKCSPNSPMTPS 378
           + P+R S A AV+  F   + T +    SP+SP+ PS
Sbjct: 263 IMPYRESFAWAVVPLFDNNLTTNTGESASPSSPLAPS 299


>At5g02500.1 68418.m00183 heat shock cognate 70 kDa protein 1
           (HSC70-1) (HSP70-1) identical to SP|P22953 Heat shock
           cognate 70 kDa protein 1 (Hsc70.1) {Arabidopsis
           thaliana}
          Length = 651

 Score = 27.1 bits (57), Expect = 7.4
 Identities = 12/42 (28%), Positives = 23/42 (54%)
 Frame = +1

Query: 307 ILGTLVIAVTSLPISEDKFFIDYSDGVIGEFGEHFEGDMVLT 432
           ++ TL+   T++P  +++ F  YSD   G   + +EG+   T
Sbjct: 415 VMTTLIPRNTTIPTKKEQVFSTYSDNQPGVLIQVYEGERART 456


>At2g39930.1 68415.m04907 isoamylase, putative / starch debranching
           enzyme, putative similar to isoamylase from [Solanum
           tuberosum] GI:27728145, [Triticum aestivum] GI:17932898,
           [Hordeum vulgare] GI:21314275, [Oryza sativa]
           GI:3252794; contains Pfam profiles PF00128: Alpha
           amylase catalytic domain, PF02922: Isoamylase N-terminal
           domain
          Length = 783

 Score = 27.1 bits (57), Expect = 7.4
 Identities = 15/34 (44%), Positives = 18/34 (52%)
 Frame = +1

Query: 325 IAVTSLPISEDKFFIDYSDGVIGEFGEHFEGDMV 426
           IAV   P+  D+FFI  SDG+   FG     D V
Sbjct: 55  IAVVEKPLKSDRFFI--SDGLPSPFGPTVRDDGV 86


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 11,199,808
Number of Sequences: 28952
Number of extensions: 223252
Number of successful extensions: 445
Number of sequences better than 10.0: 4
Number of HSP's better than 10.0 without gapping: 438
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 445
length of database: 12,070,560
effective HSP length: 76
effective length of database: 9,870,208
effective search space used: 927799552
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)