Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= S06A01NCLL0002_F24
(450 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g26830.1 68418.m03201 threonyl-tRNA synthetase / threonine--t... 147 3e-36
At1g17960.1 68414.m02222 threonyl-tRNA synthetase, putative / th... 142 7e-35
At2g04842.1 68415.m00498 threonyl-tRNA synthetase, putative / th... 31 0.48
At1g10900.1 68414.m01252 phosphatidylinositol-4-phosphate 5-kina... 30 0.63
At5g65530.1 68418.m08245 protein kinase, putative contains prote... 28 2.5
At2g42580.1 68415.m05269 tetratricopeptide repeat (TPR)-containi... 28 3.4
At1g21650.1 68414.m02710 preprotein translocase secA family prot... 27 4.4
At4g23980.1 68417.m03447 auxin-responsive factor (ARF9) contains... 27 5.9
At5g20250.2 68418.m02411 raffinose synthase family protein / see... 27 7.7
At5g20250.1 68418.m02410 raffinose synthase family protein / see... 27 7.7
At4g00440.1 68417.m00061 expressed protein 27 7.7
At3g62370.1 68416.m07006 expressed protein 27 7.7
At1g75010.1 68414.m08709 MORN (Membrane Occupation and Recogniti... 27 7.7
>At5g26830.1 68418.m03201 threonyl-tRNA synthetase / threonine--tRNA
ligase (THRRS) identical to SP|O04630 Threonyl-tRNA
synthetase, mitochondrial precursor (EC 6.1.1.3)
(Threonine--tRNA ligase) (ThrRS) {Arabidopsis thaliana}
Length = 709
Score = 147 bits (356), Expect = 3e-36
Identities = 68/135 (50%), Positives = 93/135 (68%), Gaps = 2/135 (1%)
Frame = +1
Query: 49 INKRLALWDKYKKDYEEKLAAQPEVSIVVTLPDGKTVEAKAWRTTPYDVAKGISQGLADS 228
I KR+ L+++ + + E L + P I VTLPDG E K W TTP D+A IS+GLA+S
Sbjct: 49 IPKRIKLFEQIQANQLENLKSLPHDPIKVTLPDGNVKEGKKWETTPMDIAAQISKGLANS 108
Query: 229 TIIARVNNELWDLDRPLEGDCKLELLRWDNTDAQSVFWHSSAHMLGEAMERVYGGCLCYG 408
+I+ V++ LWD++RPLEGDCKLEL ++D+ + WHSSAH+LG+A+E+ YG LC G
Sbjct: 109 ALISAVDDVLWDMNRPLEGDCKLELFKFDSDKGRDTLWHSSAHILGQALEQEYGCQLCIG 168
Query: 409 PPIE--EGFYYDMYY 447
P EGFYYD +Y
Sbjct: 169 PCTTRGEGFYYDGFY 183
>At1g17960.1 68414.m02222 threonyl-tRNA synthetase, putative /
threonine--tRNA ligase, putative similar to SP|O04630
Threonyl-tRNA synthetase, mitochondrial precursor (EC
6.1.1.3) (Threonine--tRNA ligase) (ThrRS) {Arabidopsis
thaliana}; contains Pfam profiles PF00587: tRNA
synthetase class II core domain (G, H, P, S and T),
PF03129: Anticodon binding domain, PF02824: TGS domain
Length = 458
Score = 142 bits (345), Expect = 7e-35
Identities = 65/136 (47%), Positives = 95/136 (69%), Gaps = 3/136 (2%)
Frame = +1
Query: 49 INKRLALWDKYKKDYEEKLAAQPEVSIVVTL-PDGKTVEAKAWRTTPYDVAKGISQGLAD 225
I KR+ L+++ + + E+L ++P I +TL PDG E + W T+P D+A IS+GLA
Sbjct: 17 IPKRIRLFEEIQAEQLEQLQSRPHDPIKITLLPDGIEKEGRRWETSPMDIAVQISKGLAK 76
Query: 226 STIIARVNNELWDLDRPLEGDCKLELLRWDNTDAQSVFWHSSAHMLGEAMERVYGGCLCY 405
S +++ VN+ LWD++RPLEGDC LE+ +D+ ++ FWHSSAH+LG+A+E+ YG LC
Sbjct: 77 SALVSSVNHVLWDMNRPLEGDCSLEIFGFDSDQGRNTFWHSSAHILGQALEQEYGCKLCI 136
Query: 406 GP--PIEEGFYYDMYY 447
GP P +EGFYYD Y
Sbjct: 137 GPCEPRDEGFYYDSLY 152
>At2g04842.1 68415.m00498 threonyl-tRNA synthetase, putative /
threonine--tRNA ligase, putative similar to SP|P18256
Threonyl-tRNA synthetase 2 (EC 6.1.1.3) (Threonine--tRNA
ligase) (ThrRS) {Bacillus subtilis}; contains Pfam
profiles PF00587: tRNA synthetase class II core domain
(G, H, P, S and T), PF03129: Anticodon binding domain
Length = 650
Score = 30.7 bits (66), Expect = 0.48
Identities = 12/33 (36%), Positives = 22/33 (66%), Gaps = 1/33 (3%)
Frame = +1
Query: 343 HSSAHMLGEAMERVYGGC-LCYGPPIEEGFYYD 438
H+ AH++ A+++++ + GP I+ GFYYD
Sbjct: 84 HTCAHVMAMAVQKLFPDAKVTIGPWIDNGFYYD 116
>At1g10900.1 68414.m01252 phosphatidylinositol-4-phosphate 5-kinase
family protein similar to
phosphatidylinositol-4-phosphate 5-kinase AtPIP5K1
[Arabidopsis thaliana] GI:3702691; contains Pfam
profiles PF01504: Phosphatidylinositol-4-phosphate
5-Kinase, PF02493: MORN repeat
Length = 754
Score = 30.3 bits (65), Expect = 0.63
Identities = 13/40 (32%), Positives = 20/40 (50%)
Frame = +1
Query: 289 CKLELLRWDNTDAQSVFWHSSAHMLGEAMERVYGGCLCYG 408
C+ ++RW+N D FW + G + + GCL YG
Sbjct: 141 CERGVMRWENGDLYDGFW-LNGFRHGSGVYKFADGCLYYG 179
>At5g65530.1 68418.m08245 protein kinase, putative contains protein
kinase domain, Pfam:PF00069
Length = 456
Score = 28.3 bits (60), Expect = 2.5
Identities = 15/38 (39%), Positives = 22/38 (57%)
Frame = +1
Query: 139 LPDGKTVEAKAWRTTPYDVAKGISQGLADSTIIARVNN 252
LPDG+TV K +V + +S L++ IIA VN+
Sbjct: 163 LPDGETVAIKKLTRHAKEVEERVSDFLSELGIIAHVNH 200
>At2g42580.1 68415.m05269 tetratricopeptide repeat (TPR)-containing
protein contains Pfam profile PF00515 TPR Domain
Length = 691
Score = 27.9 bits (59), Expect = 3.4
Identities = 13/28 (46%), Positives = 16/28 (57%)
Frame = -3
Query: 340 KKHSGHLCYPNAGVPTCNHLRVADPGPR 257
K HSG L P +G+PT R+ PG R
Sbjct: 90 KSHSGELSSPGSGMPTTT--RILKPGHR 115
>At1g21650.1 68414.m02710 preprotein translocase secA family protein
contains Pfam profiles: PF01043 SecA protein, amino
terminal region, PF00400 WD domain, G-beta repeat,
PF00097 zinc finger, C3HC4 type (RING finger)
Length = 1579
Score = 27.5 bits (58), Expect = 4.4
Identities = 20/91 (21%), Positives = 41/91 (45%), Gaps = 4/91 (4%)
Frame = +1
Query: 25 ELNPWPEFINKRLALWDKYKKDYEEKLAAQPEVSIVVTLPDGKTVEAKAWRTTPYDVAKG 204
E +PW F+ L + YK+D + + ++++ G+ + + W + +
Sbjct: 999 ENDPWARFVMNALKAKEFYKRDVQ--YIVRDGKALIINELTGRVEDKRRWSEGVHQAVEA 1056
Query: 205 ISQGL---ADSTIIARVN-NELWDLDRPLEG 285
+GL ADS ++A++ L+ L L G
Sbjct: 1057 -KEGLEIQADSIVVAQITYQSLFKLYPKLSG 1086
>At4g23980.1 68417.m03447 auxin-responsive factor (ARF9) contains
Pfam profiles: PF02309 AUX/IAA family and PF02362: B3
DNA binding domain
Length = 638
Score = 27.1 bits (57), Expect = 5.9
Identities = 10/18 (55%), Positives = 13/18 (72%), Gaps = 2/18 (11%)
Frame = +1
Query: 31 NPWPEFIN--KRLALWDK 78
+PWPEF N KR+ +W K
Sbjct: 587 DPWPEFCNMVKRIFIWSK 604
>At5g20250.2 68418.m02411 raffinose synthase family protein / seed
imbibition protein, putative (din10) similar to seed
imbibition protein GB:AAA32975 GI:167100 from [Hordeum
vulgare]; contains nonconsensus AT donor splice site at
intron 1; contains Pfam profile PF05691: Raffinose
synthase or seed imbibition protein Sip1; identical to
cDNA seed imbibition protein (din10) partial cds
GI:10834551
Length = 749
Score = 26.6 bits (56), Expect = 7.7
Identities = 10/22 (45%), Positives = 14/22 (63%)
Frame = +2
Query: 380 ECTEVVYAMGLPLKRGSIMTCI 445
EC + VY + LPL GS +C+
Sbjct: 116 ECNQKVYTVFLPLIEGSFRSCL 137
>At5g20250.1 68418.m02410 raffinose synthase family protein / seed
imbibition protein, putative (din10) similar to seed
imbibition protein GB:AAA32975 GI:167100 from [Hordeum
vulgare]; contains nonconsensus AT donor splice site at
intron 1; contains Pfam profile PF05691: Raffinose
synthase or seed imbibition protein Sip1; identical to
cDNA seed imbibition protein (din10) partial cds
GI:10834551
Length = 844
Score = 26.6 bits (56), Expect = 7.7
Identities = 10/22 (45%), Positives = 14/22 (63%)
Frame = +2
Query: 380 ECTEVVYAMGLPLKRGSIMTCI 445
EC + VY + LPL GS +C+
Sbjct: 211 ECNQKVYTVFLPLIEGSFRSCL 232
>At4g00440.1 68417.m00061 expressed protein
Length = 831
Score = 26.6 bits (56), Expect = 7.7
Identities = 12/25 (48%), Positives = 16/25 (64%), Gaps = 3/25 (12%)
Frame = -2
Query: 395 QPPYTLSIASPSIC---AEECQKTL 330
QPP + S+ASPS C EEC+ +
Sbjct: 571 QPPLSSSVASPSHCLAQTEECKSAI 595
>At3g62370.1 68416.m07006 expressed protein
Length = 361
Score = 26.6 bits (56), Expect = 7.7
Identities = 18/67 (26%), Positives = 31/67 (46%), Gaps = 6/67 (8%)
Frame = +1
Query: 52 NKRLALWDKYKKDYEEKLAAQPEVSIVVTLP-DGKTVEAKAWRT-----TPYDVAKGISQ 213
++ L D+ ++D + L + ++S+ P D K T TP D++ G S
Sbjct: 251 SRPLRTMDRLQQDVQFTLGSPAKMSVAFWYPVDSKPWHGSGHYTINCDWTPLDISSGSSS 310
Query: 214 GLADSTI 234
GL ST+
Sbjct: 311 GLTASTV 317
>At1g75010.1 68414.m08709 MORN (Membrane Occupation and Recognition
Nexus) repeat-containing protein
/phosphatidylinositol-4-phosphate 5-kinase-related low
similarity to phosphatidylinositol-4-phosphate 5-kinase
AtPIP5K1 [Arabidopsis thaliana] GI:3702691; contains
Pfam profile PF02493: MORN repeat
Length = 712
Score = 26.6 bits (56), Expect = 7.7
Identities = 15/50 (30%), Positives = 23/50 (46%)
Frame = -3
Query: 202 PLQHHMVWFSMLLLQQSSRLAMLPLC*LQVGQQVSLHNLSCTYPKVLNVC 53
P+ + FS L + SRLA+LP + + L+CT K +C
Sbjct: 2 PISMELPVFSTLRVPLFSRLALLPTFGVPFSSLGATTRLNCTSRKARRIC 51
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.317 0.136 0.437
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 10,717,188
Number of Sequences: 28952
Number of extensions: 227856
Number of successful extensions: 626
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 603
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 619
length of database: 12,070,560
effective HSP length: 75
effective length of database: 9,899,160
effective search space used: 732537840
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.6 bits)
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