BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= S06A01NCLL0002_E22 (487 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC1773.05c |tms1||hexitol dehydrogenase |Schizosaccharomyces p... 165 3e-42 SPCC13B11.01 |adh1|adh|alcohol dehydrogenase Adh1|Schizosaccharo... 47 1e-06 SPBC1198.01 |||glutathione-dependent formaldehyde dehydrogenase ... 46 3e-06 SPBC1539.07c |||glutathione-dependent formaldehyde dehydrogenase... 37 0.001 SPCC13B11.04c ||SPCC777.01c|glutathione-dependent formaldehyde d... 36 0.002 SPAC23G3.01 |rpb2|SPAC521.06|DNA-directed RNA polymerase II comp... 28 0.65 SPAC521.02 |||WLM domain protein|Schizosaccharomyces pombe|chr 1... 26 3.5 SPBC947.01 |||AAA family ATPase, unknown biological role|Schizos... 26 3.5 SPBC30B4.01c |wsc1|SPBC3D6.14c|transmembrane receptor Wsc1 |Schi... 25 6.1 SPAC19G12.16c |adg2|SPAC23A1.01c, mug46|conserved fungal protein... 25 8.0 SPAC144.16 |||DUF59 family protein|Schizosaccharomyces pombe|chr... 25 8.0 SPBC30D10.10c |tor1||phosphatidylinositol kinase Tor1|Schizosacc... 25 8.0 >SPBC1773.05c |tms1||hexitol dehydrogenase |Schizosaccharomyces pombe|chr 2|||Manual Length = 360 Score = 165 bits (401), Expect = 3e-42 Identities = 70/143 (48%), Positives = 90/143 (62%) Frame = +2 Query: 50 NDYEVLLRMDCVGICGSDVHYWQTGACGHFVLKEPMIMGHEXXXXXXXXXXXXXXXXXXD 229 +D++V + + GICGSDVHYW+ G G F+LK+PMI+GHE D Sbjct: 28 DDHQVKVAIKATGICGSDVHYWKEGGIGDFILKKPMILGHESAGVVVEVGKGVSSLKPGD 87 Query: 230 RVAIEPGVPCRYCEFCKTGRYHLCPDMSFCATPPVHGNLVRYYKHAADFCFKLPDHVSME 409 VA+EPG CR C++C++GRY+LCP M F ATPP G L YY DFC KLP +S+E Sbjct: 88 PVAVEPGCVCRLCDYCRSGRYNLCPHMEFAATPPYDGTLRTYYITTEDFCTKLPKQISVE 147 Query: 410 EGALLEPLSVGIHACRRGGVSAG 478 EGAL EP+SV +HA RG + G Sbjct: 148 EGALFEPMSVAVHAMTRGNLKCG 170 >SPCC13B11.01 |adh1|adh|alcohol dehydrogenase Adh1|Schizosaccharomyces pombe|chr 3|||Manual Length = 350 Score = 47.2 bits (107), Expect = 1e-06 Identities = 35/139 (25%), Positives = 56/139 (40%), Gaps = 3/139 (2%) Frame = +2 Query: 11 KDLQLVQTPIPEINDYEVLLRMDCVGICGSDVHYWQTGACGHFVL--KEPMIMGHEXXXX 184 ++++ + P+ E EVL+ + G+C +D+H Q G + L K P+I GHE Sbjct: 19 ENVKFEEVPVAEPGQDEVLVNIKYTGVCHTDLHALQ----GDWPLPAKMPLIGGHEGAGV 74 Query: 185 XXXXXXXXXXXXXXDRVAIE-PGVPCRYCEFCKTGRYHLCPDMSFCATPPVHGNLVRYYK 361 DRV ++ C CE+C +CP + V G Y Sbjct: 75 VVKVGAGVTRLKIGDRVGVKWMNSSCGNCEYCMKAEETICPHIQLSGY-TVDGTFQHYCI 133 Query: 362 HAADFCFKLPDHVSMEEGA 418 A +P+ V +E A Sbjct: 134 ANATHATIIPESVPLEVAA 152 >SPBC1198.01 |||glutathione-dependent formaldehyde dehydrogenase |Schizosaccharomyces pombe|chr 2|||Manual Length = 423 Score = 46.0 bits (104), Expect = 3e-06 Identities = 46/182 (25%), Positives = 68/182 (37%), Gaps = 24/182 (13%) Frame = +2 Query: 14 DLQLVQTPIPEI-NDYEVLLRMDCVGIC-GSDVHYWQTGACGHFVLKEPMIMGHEXXXXX 187 ++++ + P P I + +V+++ IC GSD H + G +++ I+GHE Sbjct: 47 NVKIAEVPKPTITHPKDVIVKTTACTICSGSDSHIFSGEMPG---IEKGAILGHESCGIV 103 Query: 188 XXXXXXXXXXXXXDRVAIEPGVPCRYCEFCKTGRYHLCPDMSFCATPPV-----HGNLVR 352 DRV I + C C FCK Y C + V H + Sbjct: 104 AEKGDEVNNLEIGDRVVIAFDLACGQCSFCKRHEYAACDTTNDSKLMDVNYGSHHSAIFG 163 Query: 353 YYKHAADF----------------CFKLPDHVSMEEGALL-EPLSVGIHACRRGGVSAGH 481 Y K D C KLPD + EG + + L +HAC G V G Sbjct: 164 YTKLLGDVPGCQAEYIRVPFAEINCCKLPDDIPDSEGLFMSDVLCTSLHACTLGEVKKGD 223 Query: 482 VV 487 V Sbjct: 224 TV 225 >SPBC1539.07c |||glutathione-dependent formaldehyde dehydrogenase |Schizosaccharomyces pombe|chr 2|||Manual Length = 378 Score = 37.1 bits (82), Expect = 0.001 Identities = 20/82 (24%), Positives = 37/82 (45%) Frame = +2 Query: 56 YEVLLRMDCVGICGSDVHYWQTGACGHFVLKEPMIMGHEXXXXXXXXXXXXXXXXXXDRV 235 +EV +++D +C +D Y +G P+++GHE D V Sbjct: 35 HEVRVKVDWSAVCHTDA-YTLSGVDPEGAF--PIVLGHEGAGIVESIGEGVINVRPGDHV 91 Query: 236 AIEPGVPCRYCEFCKTGRYHLC 301 + C+ C+FC++G+ +LC Sbjct: 92 ILLYTPECKECKFCRSGKTNLC 113 >SPCC13B11.04c ||SPCC777.01c|glutathione-dependent formaldehyde dehydrogenase|Schizosaccharomyces pombe|chr 3|||Manual Length = 380 Score = 36.3 bits (80), Expect = 0.002 Identities = 22/82 (26%), Positives = 37/82 (45%) Frame = +2 Query: 56 YEVLLRMDCVGICGSDVHYWQTGACGHFVLKEPMIMGHEXXXXXXXXXXXXXXXXXXDRV 235 +EV +++ G+C +D Y +G + P+I+GHE D V Sbjct: 38 HEVRIKIVNSGVCHTDA-YTLSGKDPEGLF--PVILGHEGAGIVESVGPQVTTVQVGDPV 94 Query: 236 AIEPGVPCRYCEFCKTGRYHLC 301 C+ C+FCK+G+ +LC Sbjct: 95 IALYTPECKTCKFCKSGKTNLC 116 >SPAC23G3.01 |rpb2|SPAC521.06|DNA-directed RNA polymerase II complex subunit Rpb2|Schizosaccharomyces pombe|chr 1|||Manual Length = 1210 Score = 28.3 bits (60), Expect = 0.65 Identities = 13/42 (30%), Positives = 22/42 (52%) Frame = -3 Query: 137 SDHTLQSASSVRRSHIYQRNPFAVALHNHLSQGSVFVLAVNP 12 +D T+++ S + RSH +Q F V H H + V + + P Sbjct: 1037 TDVTVEAVSKLLRSHGFQSRGFEVMYHGHTGRKLVAQVFLGP 1078 >SPAC521.02 |||WLM domain protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 283 Score = 25.8 bits (54), Expect = 3.5 Identities = 10/20 (50%), Positives = 13/20 (65%) Frame = -1 Query: 163 HDHRFFEHEVTTRSSLPVVY 104 HD RFF H T R++L +Y Sbjct: 127 HDRRFFSHLSTLRAALIALY 146 >SPBC947.01 |||AAA family ATPase, unknown biological role|Schizosaccharomyces pombe|chr 2|||Manual Length = 660 Score = 25.8 bits (54), Expect = 3.5 Identities = 13/42 (30%), Positives = 20/42 (47%) Frame = +3 Query: 30 KHRSLR*MIMKCYCEWIALVYVAPTYTTGRLERVVTSCSKNL 155 +H S R + + +W +L AP TG RV+ + NL Sbjct: 493 EHESSRRLKTEFLIQWSSLTNAAPDKQTGHSPRVLVLAATNL 534 >SPBC30B4.01c |wsc1|SPBC3D6.14c|transmembrane receptor Wsc1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 374 Score = 25.0 bits (52), Expect = 6.1 Identities = 14/59 (23%), Positives = 29/59 (49%) Frame = +3 Query: 222 SVTASLSSQASHAGTANSVKPDXXXXXXXXXXXXLRPFTATSLDITSTLPTSVSSYLTT 398 S ++S SS +S + +++S +++S +S+ P+S SS++TT Sbjct: 185 SSSSSSSSSSSSSSSSSSSSSSSVPITSSTSSSHSSSSSSSSSSSSSSRPSSSSSFITT 243 >SPAC19G12.16c |adg2|SPAC23A1.01c, mug46|conserved fungal protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 670 Score = 24.6 bits (51), Expect = 8.0 Identities = 15/59 (25%), Positives = 27/59 (45%) Frame = +3 Query: 222 SVTASLSSQASHAGTANSVKPDXXXXXXXXXXXXLRPFTATSLDITSTLPTSVSSYLTT 398 S T+S SS + +N+ PF + S I +++P+SVSS+ ++ Sbjct: 311 SFTSSSSSYTTTLTASNTSVTYTGTGTGSATFTSSPPFYSNSSVIPTSVPSSVSSFTSS 369 >SPAC144.16 |||DUF59 family protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 179 Score = 24.6 bits (51), Expect = 8.0 Identities = 9/35 (25%), Positives = 20/35 (57%) Frame = -2 Query: 465 PPRRHACIPTESGSKSAPSSIDTWSGNLKQKSAAC 361 PPR H + + G+ ++ S ++ + ++ +AAC Sbjct: 128 PPRFHVDVKVKKGTHASESQVNKQLNDKERVAAAC 162 >SPBC30D10.10c |tor1||phosphatidylinositol kinase Tor1|Schizosaccharomyces pombe|chr 2|||Manual Length = 2335 Score = 24.6 bits (51), Expect = 8.0 Identities = 12/28 (42%), Positives = 15/28 (53%) Frame = -3 Query: 140 RSDHTLQSASSVRRSHIYQRNPFAVALH 57 R DHTL V +H + N +A ALH Sbjct: 1215 REDHTLPIPIKVISAHASKCNVYAKALH 1242 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 1,937,100 Number of Sequences: 5004 Number of extensions: 33917 Number of successful extensions: 96 Number of sequences better than 10.0: 12 Number of HSP's better than 10.0 without gapping: 88 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 95 length of database: 2,362,478 effective HSP length: 68 effective length of database: 2,022,206 effective search space used: 188065158 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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