Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= S06A01NCLL0002_E12
(490 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC11G7.02 |pub1||ubiquitin-protein ligase E3|Schizosaccharomyc... 167 8e-43
SPBC16E9.11c |pub3||ubiquitin-protein ligase E3|Schizosaccharomy... 150 1e-37
SPAC1805.15c |pub2||ubiquitin-protein ligase Pub2|Schizosaccharo... 142 3e-35
SPAC19D5.04 |ptr1||HECT domain|Schizosaccharomyces pombe|chr 1||... 113 2e-26
SPAC167.07c ||SPAC57A7.03c|ubiquitin-protein ligase E3 |Schizosa... 44 9e-06
SPAC15A10.03c |rhp54|rad54|Rad54 homolog Rhp54|Schizosaccharomyc... 26 3.5
SPAC29B12.10c |||OPT oligopeptide transporter family|Schizosacch... 25 6.1
>SPAC11G7.02 |pub1||ubiquitin-protein ligase E3|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 767
Score = 167 bits (406), Expect = 8e-43
Identities = 76/139 (54%), Positives = 105/139 (75%)
Frame = +3
Query: 9 VSREWFFLLSHEVLNPMYCLFEYANKNNYSLQINPASYVNPDHLLYFKFIGRFIAMALYH 188
+SRE+FFLLSHE+ NP YCLFEY++ +NY+LQINP S +NP+HL YFKFIGR I +A++H
Sbjct: 457 LSREYFFLLSHEMFNPFYCLFEYSSVDNYTLQINPHSGINPEHLNYFKFIGRVIGLAIFH 516
Query: 189 GRFIYSGFTMPFYKRMLNKKLTMKDIESIDPEFYNSLVWIKDNNIDECGLEMWFSVDFEV 368
RF+ + F + FYK +L KK+T++D+ES+D E+Y SLVWI DN+I L++ FSV+
Sbjct: 517 RRFVDAFFVVSFYKMILQKKVTLQDMESMDAEYYRSLVWILDNDITGV-LDLTFSVEDNC 575
Query: 369 LGQVIHHELKPAGDKERVT 425
G+V+ +LKP G VT
Sbjct: 576 FGEVVTIDLKPNGRNIEVT 594
>SPBC16E9.11c |pub3||ubiquitin-protein ligase E3|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 786
Score = 150 bits (363), Expect = 1e-37
Identities = 69/139 (49%), Positives = 102/139 (73%)
Frame = +3
Query: 9 VSREWFFLLSHEVLNPMYCLFEYANKNNYSLQINPASYVNPDHLLYFKFIGRFIAMALYH 188
+SRE+FFLLSH++ +P+YCLFEY+ +NY+LQINP S +NP+HL YF+FIGR I +A++H
Sbjct: 476 LSREFFFLLSHKMFDPIYCLFEYSAVDNYTLQINPHSSINPEHLNYFRFIGRVIGLAIFH 535
Query: 189 GRFIYSGFTMPFYKRMLNKKLTMKDIESIDPEFYNSLVWIKDNNIDECGLEMWFSVDFEV 368
RF+ + F + YK++L KK+++ D+ESID EFY SL W+ +N+I L++ FSV+ +
Sbjct: 536 RRFLDAFFVVSLYKKLLRKKVSLADMESIDAEFYRSLKWVLENDITGI-LDLTFSVEEDH 594
Query: 369 LGQVIHHELKPAGDKERVT 425
G+V EL G+ VT
Sbjct: 595 FGEVRTVELITNGENIEVT 613
Score = 30.7 bits (66), Expect = 0.12
Identities = 11/28 (39%), Positives = 20/28 (71%)
Frame = +1
Query: 406 GTRRE*PESNKEQYLQVVTQWRMTRGIE 489
G E E NK++Y+ +VT+WR+++ +E
Sbjct: 607 GENIEVTEENKKKYVDLVTEWRVSKRVE 634
>SPAC1805.15c |pub2||ubiquitin-protein ligase
Pub2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 671
Score = 142 bits (344), Expect = 3e-35
Identities = 64/133 (48%), Positives = 93/133 (69%)
Frame = +3
Query: 9 VSREWFFLLSHEVLNPMYCLFEYANKNNYSLQINPASYVNPDHLLYFKFIGRFIAMALYH 188
VSRE+F++LSH + NP Y LFEYA +NY LQI+P S VNPD YF+F+GR + +A+YH
Sbjct: 361 VSREFFYILSHAIFNPGYSLFEYATDDNYGLQISPLSSVNPDFRSYFRFVGRVMGLAIYH 420
Query: 189 GRFIYSGFTMPFYKRMLNKKLTMKDIESIDPEFYNSLVWIKDNNIDECGLEMWFSVDFEV 368
R++ F +PFYKR+L K L ++D++ +D +Y SL WIK+N++DE L + FSV+
Sbjct: 421 RRYLDVQFVLPFYKRILQKPLCLEDVKDVDEVYYESLKWIKNNDVDE-SLCLNFSVEENR 479
Query: 369 LGQVIHHELKPAG 407
G+ + +L P G
Sbjct: 480 FGESVTVDLIPNG 492
>SPAC19D5.04 |ptr1||HECT domain|Schizosaccharomyces pombe|chr
1|||Manual
Length = 3227
Score = 113 bits (271), Expect = 2e-26
Identities = 58/139 (41%), Positives = 85/139 (61%)
Frame = +3
Query: 9 VSREWFFLLSHEVLNPMYCLFEYANKNNYSLQINPASYVNPDHLLYFKFIGRFIAMALYH 188
V+REW +L+ ++ NP Y LF + + N S VNPDHL +FKF GR I ALY
Sbjct: 2915 VTREWLQVLARQMFNPDYALFLPVTGDATTFHPNRDSSVNPDHLSFFKFTGRIIGKALYD 2974
Query: 189 GRFIYSGFTMPFYKRMLNKKLTMKDIESIDPEFYNSLVWIKDNNIDECGLEMWFSVDFEV 368
GR + F+ YK ML++ +++KDIES+DP++Y SLVW+ +N+I + E F+V+ +V
Sbjct: 2975 GRLLDCHFSRAVYKHMLHRSVSVKDIESLDPDYYKSLVWMLNNDITDIITEE-FAVEKDV 3033
Query: 369 LGQVIHHELKPAGDKERVT 425
G+ +L P G VT
Sbjct: 3034 FGEKTVVDLIPNGRNIPVT 3052
>SPAC167.07c ||SPAC57A7.03c|ubiquitin-protein ligase E3
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1029
Score = 44.4 bits (100), Expect = 9e-06
Identities = 37/141 (26%), Positives = 63/141 (44%), Gaps = 2/141 (1%)
Frame = +3
Query: 9 VSREWFFLLSHEVLNPMYCLFEYANKNNYSLQINPASYVNP-DHLLYFKFIGRFIAMALY 185
+++E+ + V + Y LF + + L N +Y + L ++F+G I +Y
Sbjct: 720 LTKEFLTSICKTVFDINYGLF--SETKAHLLYPNTHAYAQDVERLRCYEFLGMLIGKCIY 777
Query: 186 HGRFIYSGFTMPFYKRMLNKKLTMKDIESIDPEFYNSLVWIKDNNID-ECGLEMWFSVDF 362
G I + F F + L D+ S+DP Y LV++K+ + D E + + F+V
Sbjct: 778 EGIQIDAAFASFFVAKWLGHPSYFDDLTSLDPNLYEGLVFLKNYDGDVENDMALNFTVVH 837
Query: 363 EVLGQVIHHELKPAGDKERVT 425
E G +L P G VT
Sbjct: 838 EEFGVRNVIDLIPNGSNISVT 858
>SPAC15A10.03c |rhp54|rad54|Rad54 homolog Rhp54|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 852
Score = 25.8 bits (54), Expect = 3.5
Identities = 11/19 (57%), Positives = 13/19 (68%)
Frame = +2
Query: 254 DEGHRVYRSRVLQFVSLDK 310
DEGHR+ S L F +LDK
Sbjct: 410 DEGHRLKNSDSLTFTALDK 428
>SPAC29B12.10c |||OPT oligopeptide transporter
family|Schizosaccharomyces pombe|chr 1|||Manual
Length = 851
Score = 25.0 bits (52), Expect = 6.1
Identities = 18/56 (32%), Positives = 24/56 (42%)
Frame = +3
Query: 36 SHEVLNPMYCLFEYANKNNYSLQINPASYVNPDHLLYFKFIGRFIAMALYHGRFIY 203
S VLN Y F + +YS P SY L + F ALY+G+ I+
Sbjct: 453 SSRVLNSDYS-FNHTKYESYSPLYMPMSYSMSTALNFAAVTAIFTHCALYNGKDIW 507
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,078,157
Number of Sequences: 5004
Number of extensions: 42584
Number of successful extensions: 127
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 122
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 126
length of database: 2,362,478
effective HSP length: 68
effective length of database: 2,022,206
effective search space used: 190087364
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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