Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= S06A01NCLL0002_E12
(490 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g70320.1 68414.m08090 ubiquitin-protein ligase 2 (UPL2) nearl... 104 3e-23
At1g55860.1 68414.m06406 ubiquitin-protein ligase 1 (UPL1) nearl... 104 3e-23
At4g12570.1 68417.m01983 ubiquitin-protein ligase, putative simi... 74 5e-14
At3g17205.1 68416.m02196 HECT-domain-containing protein / ubiqui... 66 2e-11
At3g53090.1 68416.m05851 HECT-domain-containing protein / ubiqui... 60 1e-09
At5g02880.1 68418.m00231 HECT-domain-containing protein / ubiqui... 40 9e-04
At4g38600.2 68417.m05463 HECT-domain-containing protein / ubiqui... 39 0.002
At4g38600.1 68417.m05464 HECT-domain-containing protein / ubiqui... 39 0.002
At5g12260.1 68418.m01441 expressed protein 29 2.2
At5g22910.1 68418.m02679 cation/hydrogen exchanger, putative (CH... 28 2.9
At5g18700.1 68418.m02219 protein kinase-related contains protein... 28 2.9
At3g14870.1 68416.m01880 expressed protein contains Pfam profile... 27 5.1
At2g20320.1 68415.m02373 DENN (AEX-3) domain-containing protein ... 27 5.1
At2g16270.1 68415.m01863 expressed protein and genefinder; expr... 27 5.1
At5g47990.1 68418.m05929 cytochrome P450 family protein similar ... 27 6.8
At5g09360.1 68418.m01084 laccase family protein / diphenol oxida... 27 6.8
At2g42120.2 68415.m05210 DNA polymerase delta small subunit-rela... 27 6.8
At2g42120.1 68415.m05209 DNA polymerase delta small subunit-rela... 27 6.8
At5g24530.1 68418.m02897 oxidoreductase, 2OG-Fe(II) oxygenase fa... 27 9.0
>At1g70320.1 68414.m08090 ubiquitin-protein ligase 2 (UPL2) nearly
identical to ubiquitin-protein ligase 2 [Arabidopsis
thaliana] GI:7108523; E3, HECT-domain protein family;
similar to ubiquitin-protein ligase 2 GI:7108523 from
[Arabidopsis thaliana]
Length = 3658
Score = 104 bits (250), Expect = 3e-23
Identities = 56/146 (38%), Positives = 88/146 (60%), Gaps = 7/146 (4%)
Frame = +3
Query: 9 VSREWFFLLSHEVLNPMYCLFEYANKNNYSLQINPASYVNPDHLLYFKFIGRFIAMALYH 188
++REW+ LLS + + LF N+ + Q NP S +HL YFKF+GR +A AL+
Sbjct: 3340 LTREWYQLLSRVIFDKGALLFTTVG-NDATFQPNPNSVYQTEHLSYFKFVGRMVAKALFD 3398
Query: 189 GRFIYSGFTMPFYKRMLNKKLTMKDIESIDPEFYNSLVWIKDNNIDECGLEMWFSVDFE- 365
G+ + FT FYK +L K+T DIE++DP++Y +L W+ +N++ + L++ FS+D +
Sbjct: 3399 GQLLDVYFTRSFYKHILGVKVTYHDIEAVDPDYYKNLKWLLENDVSDI-LDLTFSMDADE 3457
Query: 366 ---VL---GQVIHHELKPAGDKERVT 425
+L +V +ELKP G RVT
Sbjct: 3458 EKHILYEKTEVTDYELKPGGRNIRVT 3483
>At1g55860.1 68414.m06406 ubiquitin-protein ligase 1 (UPL1) nearly
identical to ubiquitin-protein ligase 1 [Arabidopsis
thaliana] GI:7108521; E3, HECT-domain protein family;
similar to GI:7108521, GB:AAF36454 from [Arabidopsis
thaliana]
Length = 3891
Score = 104 bits (250), Expect = 3e-23
Identities = 56/146 (38%), Positives = 88/146 (60%), Gaps = 7/146 (4%)
Frame = +3
Query: 9 VSREWFFLLSHEVLNPMYCLFEYANKNNYSLQINPASYVNPDHLLYFKFIGRFIAMALYH 188
++REW+ LLS + + LF N+ + Q NP S +HL YFKF+GR +A AL+
Sbjct: 3573 LTREWYQLLSRVIFDKGALLFTTVG-NDATFQPNPNSVYQTEHLSYFKFVGRMVAKALFD 3631
Query: 189 GRFIYSGFTMPFYKRMLNKKLTMKDIESIDPEFYNSLVWIKDNNIDECGLEMWFSVDFE- 365
G+ + FT FYK +L K+T DIE++DP++Y +L W+ +N++ + L++ FS+D +
Sbjct: 3632 GQLLDVYFTRSFYKHILGVKVTYHDIEAVDPDYYKNLKWLLENDVSDI-LDLTFSMDADE 3690
Query: 366 ---VL---GQVIHHELKPAGDKERVT 425
+L +V +ELKP G RVT
Sbjct: 3691 EKHILYEKTEVTDYELKPGGRNIRVT 3716
>At4g12570.1 68417.m01983 ubiquitin-protein ligase, putative similar
to SP|P39940 Ubiquitin--protein ligase RSP5 (EC 6.3.2.-)
{Saccharomyces cerevisiae}; contains Pfam profiles
PF00240: Ubiquitin family, PF00632: HECT-domain
(ubiquitin-transferase)
Length = 873
Score = 74.1 bits (174), Expect = 5e-14
Identities = 48/142 (33%), Positives = 74/142 (52%), Gaps = 4/142 (2%)
Frame = +3
Query: 9 VSREWFFLLSHEVLNPMYCLFEYANKNNYSLQINPASYVNPDHLLYFKFIGRFIAMALYH 188
V REWF+L+ E+ NP LF + + NPAS V+P H +F+F GR IA+AL H
Sbjct: 554 VLREWFYLVCQEIFNPKNTLFLRSADDFRRFSPNPASKVDPLHPDFFEFTGRVIALALMH 613
Query: 189 GRFIYSGFTMPFYKRMLNKKLTMKDIESIDPEFYNSLVWIKDNNID----ECGLEMWFSV 356
+ F F+ ++ K++++DI+ D YNS I + + + GL + F +
Sbjct: 614 KVQVGVLFDRVFFLQLAGLKISLEDIKDTDRIMYNSCKQILEMDPEFFDSNAGLGLTFVL 673
Query: 357 DFEVLGQVIHHELKPAGDKERV 422
+ E LG+ EL P G + V
Sbjct: 674 ETEELGKRDTIELCPDGKLKAV 695
>At3g17205.1 68416.m02196 HECT-domain-containing protein /
ubiquitin-transferase family protein weak similarity to
ubiquitin-protein ligase 2 [Arabidopsis thaliana]
GI:7108523; contains Pfam profile PF00632: HECT-domain
(ubiquitin-transferase)
Length = 873
Score = 65.7 bits (153), Expect = 2e-11
Identities = 38/140 (27%), Positives = 68/140 (48%), Gaps = 1/140 (0%)
Frame = +3
Query: 9 VSREWFFLLSHEVLNPMYCLFEYANKNNYSLQINPAS-YVNPDHLLYFKFIGRFIAMALY 185
+ +++ ++ + Y LF+ ++ L NP S ++ HL +F F+G +A A++
Sbjct: 560 IFKDFMEKITRAAFDVQYGLFK--ETADHMLYPNPGSGMIHEQHLQFFHFLGSLLAKAMF 617
Query: 186 HGRFIYSGFTMPFYKRMLNKKLTMKDIESIDPEFYNSLVWIKDNNIDECGLEMWFSVDFE 365
G + F F ++ K + D+ S+DPE Y L+++K D LE++F +
Sbjct: 618 EGILVDIPFATFFLSKLKQKYNYLNDLPSLDPELYRHLIFLKRYKGDISDLELYFVILNN 677
Query: 366 VLGQVIHHELKPAGDKERVT 425
G+ EL P G RVT
Sbjct: 678 EYGERTEEELLPGGQDMRVT 697
>At3g53090.1 68416.m05851 HECT-domain-containing protein /
ubiquitin-transferase family protein / IQ
calmodulin-binding motif-containing protein contains Pfam
profiles PF00632: HECT-domain (ubiquitin-transferase),
PF00612: IQ calmodulin-binding motif
Length = 1142
Score = 59.7 bits (138), Expect = 1e-09
Identities = 36/139 (25%), Positives = 70/139 (50%)
Frame = +3
Query: 9 VSREWFFLLSHEVLNPMYCLFEYANKNNYSLQINPASYVNPDHLLYFKFIGRFIAMALYH 188
+S+E+ ++ Y LF ++ L +P++ + + +F+GR + ALY
Sbjct: 822 LSKEFLTDITKAAFATEYGLFSQTPTSDRLLVPSPSARHLENGIQMIEFLGRIVGKALYE 881
Query: 189 GRFIYSGFTMPFYKRMLNKKLTMKDIESIDPEFYNSLVWIKDNNIDECGLEMWFSVDFEV 368
G + F+ F +++L + + ++ +DPE Y +L+++K + D L + F+V E
Sbjct: 882 GILLDYSFSHVFIQKLLGRYSFIDELSGLDPELYRNLMYVKHYDGDLKELCLDFTVTEEF 941
Query: 369 LGQVIHHELKPAGDKERVT 425
G++ ELKP G VT
Sbjct: 942 CGKMSIIELKPGGKDTSVT 960
>At5g02880.1 68418.m00231 HECT-domain-containing protein /
ubiquitin-transferase family protein /
armadillo/beta-catenin-like repeat-containing protein
similar to SP|Q14669 Thyroid receptor interacting protein
12 (TRIP12) {Homo sapiens}; contains Pfam profiles
PF00632: HECT-domain (ubiquitin-transferase), PF00514:
Armadillo/beta-catenin-like repeat
Length = 1502
Score = 39.9 bits (89), Expect = 9e-04
Identities = 21/57 (36%), Positives = 31/57 (54%)
Frame = +3
Query: 132 DHLLYFKFIGRFIAMALYHGRFIYSGFTMPFYKRMLNKKLTMKDIESIDPEFYNSLV 302
D L F +G +A AL GR + + FYK +L ++L+ DI +DPE +LV
Sbjct: 1211 DVLQKFVLLGTVVAKALQDGRVLDLPLSKAFYKLILGQELSSFDIHFVDPELCKTLV 1267
>At4g38600.2 68417.m05463 HECT-domain-containing protein /
ubiquitin-transferase family protein similar to
SP|Q14669Thyroid receptor interacting protein 12 (TRIP12)
{Homo sapiens}; contains Pfam profile PF00632:
HECT-domain (ubiquitin-transferase)
Length = 1794
Score = 39.1 bits (87), Expect = 0.002
Identities = 19/52 (36%), Positives = 29/52 (55%)
Frame = +3
Query: 144 YFKFIGRFIAMALYHGRFIYSGFTMPFYKRMLNKKLTMKDIESIDPEFYNSL 299
YF+ +GR +A AL GR + + FYK +L ++L + DI D E +L
Sbjct: 1500 YFRLLGRVMAKALQDGRLLDVPLSTAFYKLILGQELDLHDIVLFDAELGKTL 1551
>At4g38600.1 68417.m05464 HECT-domain-containing protein /
ubiquitin-transferase family protein similar to
SP|Q14669Thyroid receptor interacting protein 12 (TRIP12)
{Homo sapiens}; contains Pfam profile PF00632:
HECT-domain (ubiquitin-transferase)
Length = 1888
Score = 39.1 bits (87), Expect = 0.002
Identities = 19/52 (36%), Positives = 29/52 (55%)
Frame = +3
Query: 144 YFKFIGRFIAMALYHGRFIYSGFTMPFYKRMLNKKLTMKDIESIDPEFYNSL 299
YF+ +GR +A AL GR + + FYK +L ++L + DI D E +L
Sbjct: 1591 YFRLLGRVMAKALQDGRLLDVPLSTAFYKLILGQELDLHDIVLFDAELGKTL 1642
>At5g12260.1 68418.m01441 expressed protein
Length = 624
Score = 28.7 bits (61), Expect = 2.2
Identities = 17/36 (47%), Positives = 21/36 (58%), Gaps = 2/36 (5%)
Frame = -2
Query: 471 APLCYYLKILLFI*LRSLSPCPRQVSVR--GVSLDR 370
AP + +K+L F L SLS C R +S GVS DR
Sbjct: 67 APFTFLIKVLTFNRLHSLSRCLRSLSAADYGVSGDR 102
>At5g22910.1 68418.m02679 cation/hydrogen exchanger, putative (CHX9)
monovalent cation:proton antiporter family 2 (CPA2)
member, PMID:11500563
Length = 800
Score = 28.3 bits (60), Expect = 2.9
Identities = 11/28 (39%), Positives = 16/28 (57%)
Frame = +3
Query: 12 SREWFFLLSHEVLNPMYCLFEYANKNNY 95
S+ W +L+ EVLN + + AN NY
Sbjct: 695 SKNWDYLVDDEVLNDLKATYSLANNFNY 722
>At5g18700.1 68418.m02219 protein kinase-related contains protein
kinase domain, INTERPRO:IPR000719
Length = 1366
Score = 28.3 bits (60), Expect = 2.9
Identities = 10/30 (33%), Positives = 17/30 (56%)
Frame = -2
Query: 147 NITDDRDSRNWRGLFANCNYSCSRTRIDNT 58
N+ + + N+R N N +C+ +IDNT
Sbjct: 365 NLQKENEKENYRRPLPNSNENCAEVKIDNT 394
>At3g14870.1 68416.m01880 expressed protein contains Pfam profile
PF04859: Plant protein of unknown function (DUF641
Length = 445
Score = 27.5 bits (58), Expect = 5.1
Identities = 13/50 (26%), Positives = 26/50 (52%)
Frame = +3
Query: 114 ASYVNPDHLLYFKFIGRFIAMALYHGRFIYSGFTMPFYKRMLNKKLTMKD 263
A + PD +LY+K + A+ Y + + F +P++ +KK + +D
Sbjct: 233 AELIQPD-VLYYKQDHKCFALEHYVCKIMLEAFQLPYFSNESSKKTSRED 281
>At2g20320.1 68415.m02373 DENN (AEX-3) domain-containing protein
contains Pfam domain PF02141: DENN (AEX-3) domain
Length = 976
Score = 27.5 bits (58), Expect = 5.1
Identities = 11/21 (52%), Positives = 14/21 (66%)
Frame = -3
Query: 422 HSLLVPGRFQFVVYHLTEHLE 360
HSL +P R VV+H EHL+
Sbjct: 743 HSLAIPSRGSEVVFHPLEHLQ 763
>At2g16270.1 68415.m01863 expressed protein and genefinder;
expression supported by MPSS
Length = 759
Score = 27.5 bits (58), Expect = 5.1
Identities = 22/67 (32%), Positives = 32/67 (47%)
Frame = +3
Query: 147 FKFIGRFIAMALYHGRFIYSGFTMPFYKRMLNKKLTMKDIESIDPEFYNSLVWIKDNNID 326
FKF+G F+A+AL G + S P K N+ K+I EF K NN+D
Sbjct: 310 FKFLGWFLALAL--GYLLVSATFSPLMKSSFNEFHIPKEI----TEF------AKANNLD 357
Query: 327 ECGLEMW 347
+ ++W
Sbjct: 358 QLSDKLW 364
>At5g47990.1 68418.m05929 cytochrome P450 family protein similar to
Cytochrome P450 93A3 (P450 CP5) (SP:O81973) [Glycine
max];
Length = 511
Score = 27.1 bits (57), Expect = 6.8
Identities = 18/64 (28%), Positives = 32/64 (50%), Gaps = 6/64 (9%)
Frame = +3
Query: 96 SLQINPASYVNPDHLLYFKFIGRFIA------MALYHGRFIYSGFTMPFYKRMLNKKLTM 257
SL + +S++N + Y KF+ +F+ AL R I + FYK +L+K +
Sbjct: 118 SLILGSSSFINTPYGDYSKFMKKFMVQKLLGPQALQRSRNIRADELERFYKTLLDKAMKK 177
Query: 258 KDIE 269
+ +E
Sbjct: 178 QTVE 181
>At5g09360.1 68418.m01084 laccase family protein / diphenol oxidase
family protein similar to laccase [Pinus
taeda][GI:13661201]
Length = 569
Score = 27.1 bits (57), Expect = 6.8
Identities = 22/77 (28%), Positives = 34/77 (44%), Gaps = 6/77 (7%)
Frame = +3
Query: 93 YSLQINPASYVNP--DHL-LYFKFIGRFIAMALYHG---RFIYSGFTMPFYKRMLNKKLT 254
+S IN S+VNP D L Y++ IG +F Y+G +PF R K +
Sbjct: 389 FSSSINNISFVNPSVDILRAYYRHIGGVFQEDFPRNPPTKFNYTGENLPFPTRFGTKVVV 448
Query: 255 MKDIESIDPEFYNSLVW 305
+ S++ + VW
Sbjct: 449 LDYNSSVELILQGTTVW 465
>At2g42120.2 68415.m05210 DNA polymerase delta small subunit-related
similar to DNA polymerase delta small subunit SP:Q9LRE5
from [Oryza sativa]
Length = 440
Score = 27.1 bits (57), Expect = 6.8
Identities = 13/32 (40%), Positives = 18/32 (56%)
Frame = -3
Query: 407 PGRFQFVVYHLTEHLEVNTEPHLETAFVYIVI 312
P +FQ +V H+T HL E L V++VI
Sbjct: 197 PLQFQLLVDHITGHLGDEEEQGLAAQIVHVVI 228
>At2g42120.1 68415.m05209 DNA polymerase delta small subunit-related
similar to DNA polymerase delta small subunit SP:Q9LRE5
from [Oryza sativa]
Length = 441
Score = 27.1 bits (57), Expect = 6.8
Identities = 13/32 (40%), Positives = 18/32 (56%)
Frame = -3
Query: 407 PGRFQFVVYHLTEHLEVNTEPHLETAFVYIVI 312
P +FQ +V H+T HL E L V++VI
Sbjct: 198 PLQFQLLVDHITGHLGDEEEQGLAAQIVHVVI 229
>At5g24530.1 68418.m02897 oxidoreductase, 2OG-Fe(II) oxygenase
family protein similar to flavanone 3-hydroxylase
[Persea americana][GI:727410]; contains PF03171
2OG-Fe(II) oxygenase superfamily domain
Length = 341
Score = 26.6 bits (56), Expect = 9.0
Identities = 9/16 (56%), Positives = 12/16 (75%)
Frame = -2
Query: 339 RDRIRLYCYPLSKLTN 292
RD +RL+CYP+ K N
Sbjct: 124 RDYLRLHCYPIHKYVN 139
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 10,568,561
Number of Sequences: 28952
Number of extensions: 210449
Number of successful extensions: 483
Number of sequences better than 10.0: 19
Number of HSP's better than 10.0 without gapping: 474
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 481
length of database: 12,070,560
effective HSP length: 76
effective length of database: 9,870,208
effective search space used: 848837888
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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