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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= S06A01NCLL0002_D23
         (566 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPCC191.09c |gst1||glutathione S-transferase Gst1|Schizosaccharo...    46   4e-06
SPCC965.07c |gst2||glutathione S-transferase Gst2|Schizosaccharo...    43   3e-05
SPAC688.04c |gst3||glutathione S-transferase |Schizosaccharomyce...    42   8e-05
SPBC1306.01c ||SPBC409.22c|translation elongation factor G|Schiz...    29   0.36 
SPAC6F12.05c |tnr3||thiamine diphosphokinase Tnr3 |Schizosacchar...    29   0.63 
SPAC343.16 |lys2||homoaconitate hydratase Lys2|Schizosaccharomyc...    28   1.1  
SPBC1652.01 |||conserved fungal protein|Schizosaccharomyces pomb...    27   1.5  
SPBPB21E7.01c |eno102|eno1, SPBPB8B6.07c, eno1|enolase |Schizosa...    27   1.5  
SPAC1834.01 |sup45||translation release factor eRF1|Schizosaccha...    26   3.4  
SPBC4F6.07c |||ATP-dependent RNA helicase Mak5 |Schizosaccharomy...    26   4.4  
SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr 1...    25   5.9  
SPAC2F3.01 ||SPAC323.09|mannosyltransferase complex subunit |Sch...    25   5.9  

>SPCC191.09c |gst1||glutathione S-transferase
           Gst1|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 229

 Score = 46.0 bits (104), Expect = 4e-06
 Identities = 28/67 (41%), Positives = 35/67 (52%), Gaps = 4/67 (5%)
 Frame = +2

Query: 164 EHLKPEFLKLNPQHTVPTLVD---DGLSIWESRAIITYLVNKYGKGSSL-YPEDARARAL 331
           E   PE L LNP   VPTL+D   +  +IWES AI+ YL +KY     +  P D      
Sbjct: 40  EQKSPEHLALNPNGRVPTLIDHHNNDYTIWESDAILIYLADKYDTERKISLPRDHPEYYK 99

Query: 332 VDQRLYF 352
           V Q L+F
Sbjct: 100 VIQYLFF 106


>SPCC965.07c |gst2||glutathione S-transferase
           Gst2|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 230

 Score = 43.2 bits (97), Expect = 3e-05
 Identities = 43/126 (34%), Positives = 61/126 (48%), Gaps = 15/126 (11%)
 Frame = +2

Query: 161 GEHLKPEFLKLNPQHTVPTLVD---DGLSIWESRAIITYLVNKY--GKGSSLYPEDARAR 325
           GE    E L LNP   VPTLVD   +  +IWES AI+ YL +KY   +  SL  +D    
Sbjct: 39  GEQKCKEHLALNPNGRVPTLVDHKNNDYTIWESDAILIYLADKYDTDRKISLSFDDPEYY 98

Query: 326 ALVDQRLYFDI---GTLYQRFG--DYFYPQVFAGAPAD-----KEKLAKIEDALQLLDKF 475
            L+ Q L+F     G ++ + G  ++F+ +    A        K  L  +ED L+  D +
Sbjct: 99  KLI-QYLFFQASGQGVIWGQAGWFNFFHHEPVVSAVTRYRNEIKRVLGVLEDILKDRD-Y 156

Query: 476 LDGQKY 493
           L   KY
Sbjct: 157 LVANKY 162


>SPAC688.04c |gst3||glutathione S-transferase |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 242

 Score = 41.5 bits (93), Expect = 8e-05
 Identities = 22/64 (34%), Positives = 33/64 (51%)
 Frame = +2

Query: 176 PEFLKLNPQHTVPTLVDDGLSIWESRAIITYLVNKYGKGSSLYPEDARARALVDQRLYFD 355
           P + KL+P    P +VDDG++  ES AI+ +LV KYG       ED       +  ++F 
Sbjct: 40  PAYTKLSPLGKSPIVVDDGVTYIESAAILEHLVRKYGPSFKPSEEDVAELEKYELWMHFS 99

Query: 356 IGTL 367
             +L
Sbjct: 100 EASL 103


>SPBC1306.01c ||SPBC409.22c|translation elongation factor
           G|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 770

 Score = 29.5 bits (63), Expect = 0.36
 Identities = 22/64 (34%), Positives = 35/64 (54%), Gaps = 3/64 (4%)
 Frame = -2

Query: 511 QIRAGCVLLTIEEFVQKLESVLDLGQLLFVGRRSGKYLRIEV---ISESLVEGTNVKVQA 341
           +I A  V + I +   KLE V+DL Q+  +  R  K  +IE+   + E+L+E    K  A
Sbjct: 226 KIPAAAVQIPIGQ-EDKLEGVVDLIQMRAIYNRGSKGEKIEISQQVPENLIELAKEKRSA 284

Query: 340 LIDE 329
           LI++
Sbjct: 285 LIEK 288


>SPAC6F12.05c |tnr3||thiamine diphosphokinase Tnr3
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 569

 Score = 28.7 bits (61), Expect = 0.63
 Identities = 12/32 (37%), Positives = 22/32 (68%)
 Frame = +3

Query: 345 CTLTLVPSTRDSEITSIRKYLPERLPTKRSWP 440
           CT+  +P+T++  +   R ++P R PTK++WP
Sbjct: 139 CTM-YIPATKEHPL---RIWVPRRSPTKQTWP 166


>SPAC343.16 |lys2||homoaconitate hydratase Lys2|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 721

 Score = 27.9 bits (59), Expect = 1.1
 Identities = 16/42 (38%), Positives = 23/42 (54%)
 Frame = +2

Query: 227 DGLSIWESRAIITYLVNKYGKGSSLYPEDARARALVDQRLYF 352
           + LSI ESR  I  +  ++G  S L+P D +  A  + RL F
Sbjct: 263 NSLSI-ESRLTIANMTTEWGALSGLFPTDEKLLAWYEDRLKF 303


>SPBC1652.01 |||conserved fungal protein|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 386

 Score = 27.5 bits (58), Expect = 1.5
 Identities = 19/71 (26%), Positives = 35/71 (49%), Gaps = 5/71 (7%)
 Frame = +3

Query: 258 SSLTWSTSTVKAAASTQKTLERGRSSISACTLTLVPSTRDSEITSIRK-----YLPERLP 422
           SS+ W++++  +  +  K      SS+ +   + +PS+ ++  +S+ K     YLP  L 
Sbjct: 62  SSVKWASTSFDSTVTAHKEETPYSSSLGSHDSSSLPSSTNNRYSSVLKELCNTYLPSILS 121

Query: 423 TKRSWPRSRTL 455
           T  S P  R L
Sbjct: 122 TYGSLPIRRLL 132


>SPBPB21E7.01c |eno102|eno1, SPBPB8B6.07c, eno1|enolase
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 440

 Score = 27.5 bits (58), Expect = 1.5
 Identities = 13/33 (39%), Positives = 19/33 (57%)
 Frame = +2

Query: 398 QVFAGAPADKEKLAKIEDALQLLDKFLDGQKYA 496
           Q+ +GAP   E+LAK  + L++ D   D   YA
Sbjct: 395 QMKSGAPCRSERLAKYNELLRIEDNLGDSAIYA 427


>SPAC1834.01 |sup45||translation release factor
           eRF1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 433

 Score = 26.2 bits (55), Expect = 3.4
 Identities = 8/26 (30%), Positives = 17/26 (65%)
 Frame = +2

Query: 224 DDGLSIWESRAIITYLVNKYGKGSSL 301
           +  + IW+ R ++  L+N +G G+S+
Sbjct: 6   EKAIEIWKIRRLVKQLINCHGNGTSM 31


>SPBC4F6.07c |||ATP-dependent RNA helicase Mak5 |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 648

 Score = 25.8 bits (54), Expect = 4.4
 Identities = 15/40 (37%), Positives = 22/40 (55%), Gaps = 3/40 (7%)
 Frame = +2

Query: 173 KPEFLKLNPQHTVPTLVDDGL---SIWESRAIITYLVNKY 283
           KP+FL  NPQ  V + V +GL   +  E    + YL+ +Y
Sbjct: 349 KPKFLDANPQSAVASRVLEGLIECAPAEKDLYLYYLIMRY 388


>SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 800

 Score = 25.4 bits (53), Expect = 5.9
 Identities = 32/110 (29%), Positives = 49/110 (44%), Gaps = 6/110 (5%)
 Frame = +3

Query: 255 PSSLTWSTSTVKAAASTQKTLERG----RSSISACTLTLVPSTRDSEITSIRKYLPERLP 422
           P + T STST    AST  T         +S S  T T +P T +S  T +   +P   P
Sbjct: 360 PPTSTSSTSTPPPPASTSSTGTSSSPLLSTSTSCTTSTSIPPTGNS-TTPVTPTVP---P 415

Query: 423 TKRSWPRSRTLSSFWTNSSMVKSTQPALI*P*LT--*ASSQACPASKRPI 566
           T  S P   T ++  T++S+  ++ P    P  T    +S + P +  P+
Sbjct: 416 TSSSTP--LTTTNCTTSTSVPYTSTPVTSTPLATTNCTTSTSVPYTSTPV 463


>SPAC2F3.01 ||SPAC323.09|mannosyltransferase complex subunit
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 319

 Score = 25.4 bits (53), Expect = 5.9
 Identities = 12/29 (41%), Positives = 17/29 (58%)
 Frame = -2

Query: 364 GTNVKVQALIDERPRSSVFWVEAAAFTVL 278
           GT+ KV   ID   +S +F+   AAF +L
Sbjct: 263 GTDAKVFIWIDRNSKSVLFFAFLAAFAIL 291


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,051,342
Number of Sequences: 5004
Number of extensions: 36940
Number of successful extensions: 111
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 102
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 109
length of database: 2,362,478
effective HSP length: 69
effective length of database: 2,017,202
effective search space used: 240047038
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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