Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= S06A01NCLL0002_D22
(420 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha Ef... 233 6e-63
SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha Ef... 233 6e-63
SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha Ef... 233 6e-63
SPCC584.04 |sup35|erf3|translation release factor eRF3 |Schizosa... 77 1e-15
SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related prote... 47 1e-06
SPBC9B6.04c |tuf1||mitochondrial translation elongation factor E... 27 0.90
SPAC631.01c |acp2||F-actin capping protein beta subunit |Schizos... 27 0.90
SPBC1709.17 |||folylpolyglutamate synthase|Schizosaccharomyces p... 26 2.1
SPAC15E1.05c |||ethanolamine-phosphate cytidylyltransferase |Sch... 26 2.1
SPCC777.03c |||nifs homolog|Schizosaccharomyces pombe|chr 3|||Ma... 26 2.1
SPBPJ4664.05 |||conserved fungal protein|Schizosaccharomyces pom... 25 3.6
SPAC31G5.19 |||ATPase with bromodomain protein|Schizosaccharomyc... 25 6.3
SPAC19D5.04 |ptr1||HECT domain|Schizosaccharomyces pombe|chr 1||... 25 6.3
SPAC227.12 |||U4/U6 x U5 tri-snRNP complex subunit Prp4 family|S... 25 6.3
SPBC16A3.10 |||membrane bound O-acyltransferase, MBOAT |Schizosa... 25 6.3
SPAC31A2.11c |cuf1||Cu metalloregulatory transcription factor Cu... 24 8.4
>SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha
Ef1a-b |Schizosaccharomyces pombe|chr 1|||Manual
Length = 460
Score = 233 bits (571), Expect = 6e-63
Identities = 105/137 (76%), Positives = 121/137 (88%)
Frame = +1
Query: 10 GDNVGFNVKNVSVKELRRGYVAGDSKNNPPRGAADFTAQVIVLNHPGQISNGYTPVLDCH 189
GDNVGFNVKNVSVK++RRG V GDSKN+PP G A FTAQVI+LNHPGQIS GY+PVLDCH
Sbjct: 303 GDNVGFNVKNVSVKDIRRGNVCGDSKNDPPMGCASFTAQVIILNHPGQISAGYSPVLDCH 362
Query: 190 TAHIACKFAEIKEKVDRRTGKSTEDNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGRF 369
TAHIACKFAE+ EK+DRR+GK E++PK +KSGDA I +VPSKP+CVE+F ++ PLGRF
Sbjct: 363 TAHIACKFAELIEKIDRRSGKKIEESPKFVKSGDACIAKMVPSKPMCVEAFTDYAPLGRF 422
Query: 370 AVRDMRQTVADGVIKAV 420
AVRDMRQTVA GVIKAV
Sbjct: 423 AVRDMRQTVAVGVIKAV 439
>SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha
Ef1a-a |Schizosaccharomyces pombe|chr 3|||Manual
Length = 460
Score = 233 bits (571), Expect = 6e-63
Identities = 105/137 (76%), Positives = 121/137 (88%)
Frame = +1
Query: 10 GDNVGFNVKNVSVKELRRGYVAGDSKNNPPRGAADFTAQVIVLNHPGQISNGYTPVLDCH 189
GDNVGFNVKNVSVK++RRG V GDSKN+PP G A FTAQVI+LNHPGQIS GY+PVLDCH
Sbjct: 303 GDNVGFNVKNVSVKDIRRGNVCGDSKNDPPMGCASFTAQVIILNHPGQISAGYSPVLDCH 362
Query: 190 TAHIACKFAEIKEKVDRRTGKSTEDNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGRF 369
TAHIACKFAE+ EK+DRR+GK E++PK +KSGDA I +VPSKP+CVE+F ++ PLGRF
Sbjct: 363 TAHIACKFAELIEKIDRRSGKKIEESPKFVKSGDACIAKMVPSKPMCVEAFTDYAPLGRF 422
Query: 370 AVRDMRQTVADGVIKAV 420
AVRDMRQTVA GVIKAV
Sbjct: 423 AVRDMRQTVAVGVIKAV 439
>SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha
Ef1a-c |Schizosaccharomyces pombe|chr 2|||Manual
Length = 460
Score = 233 bits (571), Expect = 6e-63
Identities = 105/137 (76%), Positives = 121/137 (88%)
Frame = +1
Query: 10 GDNVGFNVKNVSVKELRRGYVAGDSKNNPPRGAADFTAQVIVLNHPGQISNGYTPVLDCH 189
GDNVGFNVKNVSVK++RRG V GDSKN+PP G A FTAQVI+LNHPGQIS GY+PVLDCH
Sbjct: 303 GDNVGFNVKNVSVKDIRRGNVCGDSKNDPPMGCASFTAQVIILNHPGQISAGYSPVLDCH 362
Query: 190 TAHIACKFAEIKEKVDRRTGKSTEDNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGRF 369
TAHIACKFAE+ EK+DRR+GK E++PK +KSGDA I +VPSKP+CVE+F ++ PLGRF
Sbjct: 363 TAHIACKFAELIEKIDRRSGKKIEESPKFVKSGDACIAKMVPSKPMCVEAFTDYAPLGRF 422
Query: 370 AVRDMRQTVADGVIKAV 420
AVRDMRQTVA GVIKAV
Sbjct: 423 AVRDMRQTVAVGVIKAV 439
>SPCC584.04 |sup35|erf3|translation release factor eRF3
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 662
Score = 77.0 bits (181), Expect = 1e-15
Identities = 43/134 (32%), Positives = 69/134 (51%)
Frame = +1
Query: 10 GDNVGFNVKNVSVKELRRGYVAGDSKNNPPRGAADFTAQVIVLNHPGQISNGYTPVLDCH 189
GD V V+ +++ GYV +KN P F AQ+ +L P ++ GY+ V+ H
Sbjct: 527 GDQVRLRVRGDD-SDVQTGYVLTSTKN-PVHATTRFIAQIAILELPSILTTGYSCVMHIH 584
Query: 190 TAHIACKFAEIKEKVDRRTGKSTEDNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGRF 369
TA FA++ K+D +T + ++ P G I L P+C+E F+++ +GRF
Sbjct: 585 TAVEEVSFAKLLHKLD-KTNRKSKKPPMFATKGMKIIAELETQTPVCMERFEDYQYMGRF 643
Query: 370 AVRDMRQTVADGVI 411
+RD TVA G +
Sbjct: 644 TLRDQGTTVAVGKV 657
>SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related
protein|Schizosaccharomyces pombe|chr 2|||Manual
Length = 592
Score = 47.2 bits (107), Expect = 1e-06
Identities = 41/138 (29%), Positives = 62/138 (44%)
Frame = +1
Query: 1 ARGGDNVGFNVKNVSVKELRRGYVAGDSKNNPPRGAADFTAQVIVLNHPGQISNGYTPVL 180
A GD V + ++ V +LR G + + +N P R F A++ + G I +G T VL
Sbjct: 461 AVAGDTVTLQLADIEVNQLRPGDILSNYEN-PVRRVRSFVAEIQTFDIHGPILSGSTLVL 519
Query: 181 DCHTAHIACKFAEIKEKVDRRTGKSTEDNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPL 360
H+ + K+ K + + S K I L PLC+ +E P L
Sbjct: 520 -----HLGRTVTSVSLKIVTVNNKRSR-HIASRKRALVRISFLDGLFPLCLA--EECPAL 571
Query: 361 GRFAVRDMRQTVADGVIK 414
GRF +R TVA G++K
Sbjct: 572 GRFILRRSGDTVAAGIVK 589
>SPBC9B6.04c |tuf1||mitochondrial translation elongation factor
EF-Tu Tuf1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 439
Score = 27.5 bits (58), Expect = 0.90
Identities = 28/137 (20%), Positives = 55/137 (40%)
Frame = +1
Query: 1 ARGGDNVGFNVKNVSVKELRRGYVAGDSKNNPPRGAADFTAQVIVLNHPGQISNGYTPVL 180
A GDN G ++++ ++L+RG + P F A +L + T +
Sbjct: 311 AVAGDNCGLLLRSIKREQLKRGMIVAQPGTVAPH--QKFKASFYILTK--EEGGRRTGFV 366
Query: 181 DCHTAHIACKFAEIKEKVDRRTGKSTEDNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPL 360
D + + + +++ ++ T D+ K + GD + P+ +E Q
Sbjct: 367 DKYRPQLYSRTSDVTVEL---THPDPNDSDKMVMPGDNVEMICTLIHPIVIEKGQ----- 418
Query: 361 GRFAVRDMRQTVADGVI 411
RF VR+ TV ++
Sbjct: 419 -RFTVREGGSTVGTALV 434
>SPAC631.01c |acp2||F-actin capping protein beta subunit
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 268
Score = 27.5 bits (58), Expect = 0.90
Identities = 12/25 (48%), Positives = 19/25 (76%)
Frame = -2
Query: 299 MAASPDLMDLGLSSVDLPVRRSTFS 225
++ +PDL D+ LSSVD P++ +T S
Sbjct: 27 LSVAPDLADVLLSSVDQPLKVNTCS 51
>SPBC1709.17 |||folylpolyglutamate synthase|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 505
Score = 26.2 bits (55), Expect = 2.1
Identities = 15/48 (31%), Positives = 24/48 (50%), Gaps = 1/48 (2%)
Frame = +3
Query: 99 QGSCRLHSASHCAKSPRSNIKRIHTCIGLPHSPHSLQIC-RNQRESRP 239
+GS ++S + +S + I CIG+ SPH +C R Q +P
Sbjct: 90 KGSTCAFTSSILQQIQKSGERSIPKCIGMYTSPHLRSVCERIQLNGKP 137
>SPAC15E1.05c |||ethanolamine-phosphate cytidylyltransferase
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 365
Score = 26.2 bits (55), Expect = 2.1
Identities = 15/54 (27%), Positives = 28/54 (51%)
Frame = -2
Query: 320 LEGTRLTMAASPDLMDLGLSSVDLPVRRSTFSLISANLQAMWAVWQSNTGVYPF 159
LE R ++ +L+D LSSV L + + S++S+ + + + G+ PF
Sbjct: 122 LEVKRTEGVSTTELLDRLLSSVPLEIYSTPVSVLSSQIDLLRRFATDSDGLTPF 175
>SPCC777.03c |||nifs homolog|Schizosaccharomyces pombe|chr
3|||Manual
Length = 396
Score = 26.2 bits (55), Expect = 2.1
Identities = 14/42 (33%), Positives = 22/42 (52%), Gaps = 2/42 (4%)
Frame = -2
Query: 284 DLMDLGLSSVDL--PVRRSTFSLISANLQAMWAVWQSNTGVY 165
+L LG++ + P +RST S ++ L W + N GVY
Sbjct: 316 ELSKLGVTIIGSKDPKKRSTHSYVAKILNPEWDAFLKNEGVY 357
>SPBPJ4664.05 |||conserved fungal protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 163
Score = 25.4 bits (53), Expect = 3.6
Identities = 11/34 (32%), Positives = 19/34 (55%)
Frame = -1
Query: 243 TTVDFLFDFGKFAGYVGCVAIQYRCVSV*YLTWV 142
T +D+LF F+ +G + Y ++V Y+ WV
Sbjct: 73 TLIDYLFFSPPFSLSIGPSLLVYLSIAVSYMLWV 106
>SPAC31G5.19 |||ATPase with bromodomain protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1190
Score = 24.6 bits (51), Expect = 6.3
Identities = 15/47 (31%), Positives = 24/47 (51%), Gaps = 3/47 (6%)
Frame = +2
Query: 272 NPLNLVMPPLSTWFPPSPCVWS---PSRNSHPSVVSPCVT*GRRSPT 403
+PL+ + P L WF V+S P+R+S + P + + SPT
Sbjct: 707 SPLSELHPQLREWFSSKQSVYSLQYPTRDSIIAFFQPILELIKASPT 753
>SPAC19D5.04 |ptr1||HECT domain|Schizosaccharomyces pombe|chr
1|||Manual
Length = 3227
Score = 24.6 bits (51), Expect = 6.3
Identities = 9/30 (30%), Positives = 18/30 (60%)
Frame = -1
Query: 354 WEFLEGLHTQGLGGNQVDNGGITRFNGFRV 265
++FL+ + + LGGN + G+T G+ +
Sbjct: 389 YDFLQFMTSSPLGGNMACSAGLTSLLGYHL 418
>SPAC227.12 |||U4/U6 x U5 tri-snRNP complex subunit Prp4
family|Schizosaccharomyces pombe|chr 1|||Manual
Length = 462
Score = 24.6 bits (51), Expect = 6.3
Identities = 8/19 (42%), Positives = 12/19 (63%)
Frame = -2
Query: 224 LISANLQAMWAVWQSNTGV 168
L+SA+ W +W +TGV
Sbjct: 280 LVSASFDTTWRLWDVHTGV 298
>SPBC16A3.10 |||membrane bound O-acyltransferase, MBOAT
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 509
Score = 24.6 bits (51), Expect = 6.3
Identities = 9/31 (29%), Positives = 17/31 (54%)
Frame = -1
Query: 96 WVVFRISCNVTTAQFLDRYVFDVETNIVTAS 4
W++F + T + + RY++ E +TAS
Sbjct: 95 WIIFIVILGHTFSSHVIRYIYPSENTDITAS 125
>SPAC31A2.11c |cuf1||Cu metalloregulatory transcription factor Cuf1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 411
Score = 24.2 bits (50), Expect = 8.4
Identities = 11/25 (44%), Positives = 14/25 (56%)
Frame = +1
Query: 136 LNHPGQISNGYTPVLDCHTAHIACK 210
L HP Q+SN +T C A AC+
Sbjct: 305 LPHPIQLSNYFTLPSSCAQADAACQ 329
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.317 0.135 0.396
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,998,158
Number of Sequences: 5004
Number of extensions: 43583
Number of successful extensions: 119
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 116
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 118
length of database: 2,362,478
effective HSP length: 66
effective length of database: 2,032,214
effective search space used: 148351622
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.6 bits)
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