Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= S06A01NCLL0002_C17
(416 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g21240.1 68416.m02684 4-coumarate--CoA ligase 2 / 4-coumaroyl... 72 1e-13
At1g65060.2 68414.m07376 4-coumarate--CoA ligase 3 / 4-coumaroyl... 71 3e-13
At1g65060.1 68414.m07375 4-coumarate--CoA ligase 3 / 4-coumaroyl... 71 3e-13
At1g51680.2 68414.m05823 4-coumarate--CoA ligase 1 / 4-coumaroyl... 68 3e-12
At1g51680.1 68414.m05822 4-coumarate--CoA ligase 1 / 4-coumaroyl... 68 3e-12
At1g20510.2 68414.m02556 4-coumarate--CoA ligase family protein ... 67 5e-12
At1g20510.1 68414.m02555 4-coumarate--CoA ligase family protein ... 67 5e-12
At1g20500.1 68414.m02554 4-coumarate--CoA ligase family / 4-coum... 66 7e-12
At5g63380.1 68418.m07955 4-coumarate--CoA ligase family protein ... 65 2e-11
At3g21230.1 68416.m02683 4-coumarate--CoA ligase, putative / 4-c... 65 2e-11
At4g05160.1 68417.m00775 4-coumarate--CoA ligase, putative / 4-c... 64 5e-11
At1g20490.1 68414.m02553 AMP-dependent synthetase and ligase fam... 63 6e-11
At5g38120.1 68418.m04592 4-coumarate--CoA ligase family protein ... 62 1e-10
At1g62940.1 68414.m07107 4-coumarate--CoA ligase family protein ... 60 6e-10
At4g19010.1 68417.m02802 4-coumarate--CoA ligase family protein ... 57 4e-09
At4g14070.1 68417.m02172 AMP-binding protein, putative similar t... 54 4e-08
At1g20480.1 68414.m02552 4-coumarate--CoA ligase family protein ... 54 4e-08
At3g23790.1 68416.m02990 AMP-binding protein, putative similar t... 48 3e-06
At2g04350.2 68415.m00434 long-chain-fatty-acid--CoA ligase famil... 46 1e-05
At2g04350.1 68415.m00433 long-chain-fatty-acid--CoA ligase famil... 46 1e-05
At3g05970.1 68416.m00681 long-chain-fatty-acid--CoA ligase / lon... 45 2e-05
At1g30520.1 68414.m03734 acyl-activating enzyme 14 (AAE14) ident... 43 1e-04
At1g49430.1 68414.m05541 long-chain-fatty-acid--CoA ligase / lon... 42 1e-04
At3g16170.1 68416.m02041 acyl-activating enzyme 13 (AAE13) simil... 42 2e-04
At3g48990.1 68416.m05351 AMP-dependent synthetase and ligase fam... 41 4e-04
At2g47240.1 68415.m05899 long-chain-fatty-acid--CoA ligase famil... 41 4e-04
At3g16910.1 68416.m02162 AMP-dependent synthetase and ligase fam... 40 5e-04
At1g77590.1 68414.m09034 long-chain-fatty-acid--CoA ligase famil... 40 7e-04
At5g27600.1 68418.m03305 AMP-binding protein, putative similar t... 38 0.003
At1g77240.1 68414.m08996 AMP-binding protein, putative strong si... 37 0.005
At1g20560.1 68414.m02563 AMP-dependent synthetase and ligase fam... 37 0.006
At4g11030.1 68417.m01794 long-chain-fatty-acid--CoA ligase, puta... 36 0.008
At5g36880.1 68418.m04418 acetyl-CoA synthetase, putative / aceta... 36 0.014
At1g66120.1 68414.m07504 acyl-activating enzyme 11 (AAE11) simil... 33 0.077
At2g17650.1 68415.m02042 AMP-dependent synthetase and ligase fam... 33 0.10
At4g23850.1 68417.m03429 long-chain-fatty-acid--CoA ligase / lon... 32 0.14
At1g64400.1 68414.m07299 long-chain-fatty-acid--CoA ligase, puta... 32 0.14
At1g21540.1 68414.m02694 AMP-binding protein, putative strong si... 32 0.14
At1g21530.1 68414.m02693 AMP-binding protein, putative strong si... 32 0.18
At5g23050.1 68418.m02695 acyl-activating enzyme 17 (AAE17) nearl... 31 0.24
At5g16340.1 68418.m01910 AMP-binding protein, putative similar t... 31 0.31
At1g65880.1 68414.m07476 AMP-dependent synthetase and ligase fam... 31 0.31
At5g16370.1 68418.m01913 AMP-binding protein, putative similar t... 31 0.41
At1g75960.1 68414.m08822 AMP-binding protein, putative similar t... 30 0.54
At1g68270.1 68414.m07798 AMP-dependent synthetase and ligase fam... 30 0.54
At1g65890.1 68414.m07477 acyl-activating enzyme 12 (AAE12) simil... 30 0.54
At5g35930.1 68418.m04322 AMP-dependent synthetase and ligase fam... 30 0.72
At1g55320.1 68414.m06319 acyl-activating enzyme 18 (AAE18) nearl... 29 0.95
At5g14880.1 68418.m01745 potassium transporter, putative similar... 27 5.1
At5g49530.1 68418.m06130 SIN-like family protein low similarity ... 27 6.7
At1g21410.1 68414.m02679 F-box family protein similar to SKP1 i... 26 8.9
At1g20720.1 68414.m02596 helicase-related similar to BRCA1-bindi... 26 8.9
>At3g21240.1 68416.m02684 4-coumarate--CoA ligase 2 /
4-coumaroyl-CoA synthase 2 (4CL2) identical to SP|Q9S725
4-coumarate--CoA ligase 2 (EC 6.2.1.12) (4CL 2)
(4-coumaroyl-CoA synthase 2) {Arabidopsis thaliana}
Length = 556
Score = 72.1 bits (169), Expect = 1e-13
Identities = 42/103 (40%), Positives = 59/103 (57%), Gaps = 4/103 (3%)
Frame = +1
Query: 118 DTVFILFSSGTTGLPKGVMLTH---INALCAAMNFENDNMHEDKFKTMLSIVPWFHAYGL 288
D V + FSSGTTGLPKGVMLTH + ++ ++ EN N++ ++ +L ++P FH Y L
Sbjct: 196 DVVALPFSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFNRDDVILCVLPMFHIYAL 255
Query: 289 ISTINYIL-IKKTIVYFSGFNPQKYLNAIQEYKVNVLVAVPPI 414
S + L + TI+ F L IQ KV V + VPPI
Sbjct: 256 NSIMLCSLRVGATILIMPKFEITLLLEQIQRCKVTVAMVVPPI 298
>At1g65060.2 68414.m07376 4-coumarate--CoA ligase 3 /
4-coumaroyl-CoA synthase 3 (4CL3) identical to SP|Q9S777
4-coumarate--CoA ligase 3 (EC 6.2.1.12) (4CL 3)
(4-coumaroyl-CoA synthase 3) {Arabidopsis thaliana}
Length = 495
Score = 70.9 bits (166), Expect = 3e-13
Identities = 39/134 (29%), Positives = 68/134 (50%), Gaps = 4/134 (2%)
Frame = +1
Query: 25 KPLESGIISYSDIKVETDVRSYEPAEVQGWTDTVFILFSSGTTGLPKGVMLTH---INAL 195
+P + +S + + + ++ G D + FSSGTTGLPKGV+LTH I ++
Sbjct: 175 EPTPENCLPFSTLITDDETNPFQETVDIGGDDAAALPFSSGTTGLPKGVVLTHKSLITSV 234
Query: 196 CAAMNFENDNMHEDKFKTMLSIVPWFHAYGLIST-INYILIKKTIVYFSGFNPQKYLNAI 372
++ +N N++ +L ++P FH Y L S +N + T++ F L+ I
Sbjct: 235 AQQVDGDNPNLYLKSNDVILCVLPLFHIYSLNSVLLNSLRSGATVLLMHKFEIGALLDLI 294
Query: 373 QEYKVNVLVAVPPI 414
Q ++V + VPP+
Sbjct: 295 QRHRVTIAALVPPL 308
>At1g65060.1 68414.m07375 4-coumarate--CoA ligase 3 /
4-coumaroyl-CoA synthase 3 (4CL3) identical to SP|Q9S777
4-coumarate--CoA ligase 3 (EC 6.2.1.12) (4CL 3)
(4-coumaroyl-CoA synthase 3) {Arabidopsis thaliana}
Length = 561
Score = 70.9 bits (166), Expect = 3e-13
Identities = 39/134 (29%), Positives = 68/134 (50%), Gaps = 4/134 (2%)
Frame = +1
Query: 25 KPLESGIISYSDIKVETDVRSYEPAEVQGWTDTVFILFSSGTTGLPKGVMLTH---INAL 195
+P + +S + + + ++ G D + FSSGTTGLPKGV+LTH I ++
Sbjct: 175 EPTPENCLPFSTLITDDETNPFQETVDIGGDDAAALPFSSGTTGLPKGVVLTHKSLITSV 234
Query: 196 CAAMNFENDNMHEDKFKTMLSIVPWFHAYGLIST-INYILIKKTIVYFSGFNPQKYLNAI 372
++ +N N++ +L ++P FH Y L S +N + T++ F L+ I
Sbjct: 235 AQQVDGDNPNLYLKSNDVILCVLPLFHIYSLNSVLLNSLRSGATVLLMHKFEIGALLDLI 294
Query: 373 QEYKVNVLVAVPPI 414
Q ++V + VPP+
Sbjct: 295 QRHRVTIAALVPPL 308
>At1g51680.2 68414.m05823 4-coumarate--CoA ligase 1 /
4-coumaroyl-CoA synthase 1 (4CL1) identical to SP|Q42524
4-coumarate--CoA ligase 1 (EC 6.2.1.12) (4CL 1)
(4-coumaroyl-CoA synthase 1) {Arabidopsis thaliana}
Length = 490
Score = 67.7 bits (158), Expect = 3e-12
Identities = 45/134 (33%), Positives = 67/134 (50%), Gaps = 5/134 (3%)
Frame = +1
Query: 28 PLESGIISYSDIKVETDVRSYEPAEVQGWTDTVFIL-FSSGTTGLPKGVMLTH---INAL 195
P+ G + ++++ T S V+ D V L +SSGTTGLPKGVMLTH + ++
Sbjct: 172 PIPEGCLRFTELTQSTTEASEVIDSVEISPDDVVALPYSSGTTGLPKGVMLTHKGLVTSV 231
Query: 196 CAAMNFENDNMHEDKFKTMLSIVPWFHAYGLIS-TINYILIKKTIVYFSGFNPQKYLNAI 372
++ EN N++ +L ++P FH Y L S + + + I+ F L I
Sbjct: 232 AQQVDGENPNLYFHSDDVILCVLPMFHIYALNSIMLCGLRVGAAILIMPKFEINLLLELI 291
Query: 373 QEYKVNVLVAVPPI 414
Q KV V VPPI
Sbjct: 292 QRCKVTVAPMVPPI 305
>At1g51680.1 68414.m05822 4-coumarate--CoA ligase 1 /
4-coumaroyl-CoA synthase 1 (4CL1) identical to SP|Q42524
4-coumarate--CoA ligase 1 (EC 6.2.1.12) (4CL 1)
(4-coumaroyl-CoA synthase 1) {Arabidopsis thaliana}
Length = 561
Score = 67.7 bits (158), Expect = 3e-12
Identities = 45/134 (33%), Positives = 67/134 (50%), Gaps = 5/134 (3%)
Frame = +1
Query: 28 PLESGIISYSDIKVETDVRSYEPAEVQGWTDTVFIL-FSSGTTGLPKGVMLTH---INAL 195
P+ G + ++++ T S V+ D V L +SSGTTGLPKGVMLTH + ++
Sbjct: 172 PIPEGCLRFTELTQSTTEASEVIDSVEISPDDVVALPYSSGTTGLPKGVMLTHKGLVTSV 231
Query: 196 CAAMNFENDNMHEDKFKTMLSIVPWFHAYGLIS-TINYILIKKTIVYFSGFNPQKYLNAI 372
++ EN N++ +L ++P FH Y L S + + + I+ F L I
Sbjct: 232 AQQVDGENPNLYFHSDDVILCVLPMFHIYALNSIMLCGLRVGAAILIMPKFEINLLLELI 291
Query: 373 QEYKVNVLVAVPPI 414
Q KV V VPPI
Sbjct: 292 QRCKVTVAPMVPPI 305
>At1g20510.2 68414.m02556 4-coumarate--CoA ligase family protein /
4-coumaroyl-CoA synthase family protein similar to
SP|P14912 and SP|P14913 from Petroselinum crispum;
contains Pfam AMP-binding enzyme domain PF00501
Length = 473
Score = 66.9 bits (156), Expect = 5e-12
Identities = 42/133 (31%), Positives = 65/133 (48%), Gaps = 6/133 (4%)
Frame = +1
Query: 34 ESGIISYSDIKVETDVRSYEPA-----EVQGWTDTVFILFSSGTTGLPKGVMLTHINALC 198
E + S D++ ++ EP+ E DT +L+SSGTTG+ KGV+ +H N +
Sbjct: 157 EERVDSVGDVRRLVEMMKKEPSGNRVKERVDQDDTATLLYSSGTTGMSKGVISSHRNLIA 216
Query: 199 AAMNFENDNMHEDKFKTMLSIVPWFHAYGLISTINYIL-IKKTIVYFSGFNPQKYLNAIQ 375
N +D + + VP FH YGL + +L TI+ S F + ++AI
Sbjct: 217 MVQTIVNRFGSDDGEQRFICTVPMFHIYGLAAFATGLLAYGSTIIVLSKFEMHEMMSAIG 276
Query: 376 EYKVNVLVAVPPI 414
+Y+ L VPPI
Sbjct: 277 KYQATSLPLVPPI 289
>At1g20510.1 68414.m02555 4-coumarate--CoA ligase family protein /
4-coumaroyl-CoA synthase family protein similar to
SP|P14912 and SP|P14913 from Petroselinum crispum;
contains Pfam AMP-binding enzyme domain PF00501
Length = 546
Score = 66.9 bits (156), Expect = 5e-12
Identities = 42/133 (31%), Positives = 65/133 (48%), Gaps = 6/133 (4%)
Frame = +1
Query: 34 ESGIISYSDIKVETDVRSYEPA-----EVQGWTDTVFILFSSGTTGLPKGVMLTHINALC 198
E + S D++ ++ EP+ E DT +L+SSGTTG+ KGV+ +H N +
Sbjct: 157 EERVDSVGDVRRLVEMMKKEPSGNRVKERVDQDDTATLLYSSGTTGMSKGVISSHRNLIA 216
Query: 199 AAMNFENDNMHEDKFKTMLSIVPWFHAYGLISTINYIL-IKKTIVYFSGFNPQKYLNAIQ 375
N +D + + VP FH YGL + +L TI+ S F + ++AI
Sbjct: 217 MVQTIVNRFGSDDGEQRFICTVPMFHIYGLAAFATGLLAYGSTIIVLSKFEMHEMMSAIG 276
Query: 376 EYKVNVLVAVPPI 414
+Y+ L VPPI
Sbjct: 277 KYQATSLPLVPPI 289
>At1g20500.1 68414.m02554 4-coumarate--CoA ligase family /
4-coumaroyl-CoA synthase family similar to SP|Q42524 and
SP|Q9S725; contains Pfam AMP-binding enzyme domain
PF00501
Length = 412
Score = 66.5 bits (155), Expect = 7e-12
Identities = 32/100 (32%), Positives = 53/100 (53%), Gaps = 1/100 (1%)
Frame = +1
Query: 118 DTVFILFSSGTTGLPKGVMLTHINALCAAMNFENDNMHEDKFKTMLSIVPWFHAYGLIS- 294
DT +L+SSGTTG KGV+ +H N F +DN+ D + VP FH YGL++
Sbjct: 198 DTAMMLYSSGTTGPSKGVISSHRNLTAHVARFISDNLKRDDI--FICTVPMFHTYGLLTF 255
Query: 295 TINYILIKKTIVYFSGFNPQKYLNAIQEYKVNVLVAVPPI 414
+ + + T+V F ++A+++++ L PP+
Sbjct: 256 AMGTVALGSTVVILRRFQLHDMMDAVEKHRATALALAPPV 295
>At5g63380.1 68418.m07955 4-coumarate--CoA ligase family protein /
4-coumaroyl-CoA synthase family protein similar to 4CL2
[gi:12229665] from Arabidopsis thaliana, 4CL1
[gi:12229631] from Nicotiana tabacum; contains Pfam
AMP-binding enzyme domain PF00501
Length = 562
Score = 65.3 bits (152), Expect = 2e-11
Identities = 38/117 (32%), Positives = 60/117 (51%), Gaps = 3/117 (2%)
Frame = +1
Query: 73 TDVRSYEPAEVQ-GWTDTVFILFSSGTTGLPKGVMLTHINALCAAMNFENDNMHE--DKF 243
+D S P +VQ +D ILFSSGTTG KGV+LTH N + + + + +
Sbjct: 189 SDSSSVNPFQVQVNQSDPAAILFSSGTTGRVKGVLLTHRNLIASTAVSHQRTLQDPVNYD 248
Query: 244 KTMLSIVPWFHAYGLISTINYILIKKTIVYFSGFNPQKYLNAIQEYKVNVLVAVPPI 414
+ L +P FH +G + I I + +T+V F + A+++YKV + PP+
Sbjct: 249 RVGLFSLPLFHVFGFMMMIRAISLGETLVLLGRFELEAMFKAVEKYKVTGMPVSPPL 305
>At3g21230.1 68416.m02683 4-coumarate--CoA ligase, putative /
4-coumaroyl-CoA synthase, putative (4CL) similar to 4CL2
[gi:12229665] and 4CL1 [gi:12229649] from [Arabidopsis
thaliana], 4CL1 [gi:12229631] from Nicotiana tabacum
Length = 570
Score = 65.3 bits (152), Expect = 2e-11
Identities = 38/131 (29%), Positives = 67/131 (51%), Gaps = 4/131 (3%)
Frame = +1
Query: 34 ESGIISYSDIKVETDVRSYEPAEVQGWTDTVFILFSSGTTGLPKGVMLTH---INALCAA 204
+ G +S++++ + +P DTV + +SSGTTGLPKGVM+TH + ++
Sbjct: 185 DDGCVSFTELTQADETELLKPKISP--EDTVAMPYSSGTTGLPKGVMITHKGLVTSIAQK 242
Query: 205 MNFENDNMHEDKFKTMLSIVPWFHAYGLIS-TINYILIKKTIVYFSGFNPQKYLNAIQEY 381
++ EN N++ +L +P FH Y L + ++ + ++ F + IQ Y
Sbjct: 243 VDGENPNLNFTANDVILCFLPMFHIYALDALMLSAMRTGAALLIVPRFELNLVMELIQRY 302
Query: 382 KVNVLVAVPPI 414
KV V+ PP+
Sbjct: 303 KVTVVPVAPPV 313
>At4g05160.1 68417.m00775 4-coumarate--CoA ligase, putative /
4-coumaroyl-CoA synthase, putative similar to 4CL2
[gi:12229665] from Arabidopsis thaliana, 4CL1
[gi:12229631] from Nicotiana tabacum; contains Pfam
AMP-binding enzyme domain PF00501; acyl-activating
enzyme superfamily; identical to cDNA 4-coumarate-CoA
ligase-like protein (At4g05160) GI:29893226
Length = 544
Score = 63.7 bits (148), Expect = 5e-11
Identities = 40/129 (31%), Positives = 70/129 (54%), Gaps = 3/129 (2%)
Frame = +1
Query: 37 SGIISYSDI-KVETDVRSYEPAEVQGWTDTVFILFSSGTTGLPKGVMLTHINALCAAMNF 213
S I+S+ ++ ++ V Y E++ +DT +L+SSGTTG KGV LTH N + A++
Sbjct: 164 SKILSFDNVMELSEPVSEYPFVEIKQ-SDTAALLYSSGTTGTSKGVELTHGNFIAASLMV 222
Query: 214 END-NMHEDKFKTMLSIVPWFHAYGL-ISTINYILIKKTIVYFSGFNPQKYLNAIQEYKV 387
D ++ + L +P FH +GL + T + + +V + F + L I++++V
Sbjct: 223 TMDQDLMGEYHGVFLCFLPMFHVFGLAVITYSQLQRGNALVSMARFELELVLKNIEKFRV 282
Query: 388 NVLVAVPPI 414
L VPP+
Sbjct: 283 THLWVVPPV 291
>At1g20490.1 68414.m02553 AMP-dependent synthetase and ligase family
protein similar to SP|Q42524 and SP|Q9S725; contains
Pfam AMP-binding enzyme domain PF00501
Length = 447
Score = 63.3 bits (147), Expect = 6e-11
Identities = 31/100 (31%), Positives = 52/100 (52%), Gaps = 1/100 (1%)
Frame = +1
Query: 118 DTVFILFSSGTTGLPKGVMLTHINALCAAMNFENDNMHEDKFKTMLSIVPWFHAYGLIS- 294
DT +L+SSGTTG KGV+ +H N + +D D+ + VP FH++GL++
Sbjct: 200 DTAMMLYSSGTTGTSKGVISSHRNLTAYVAKYIDDKWKRDEI--FVCTVPMFHSFGLLAF 257
Query: 295 TINYILIKKTIVYFSGFNPQKYLNAIQEYKVNVLVAVPPI 414
+ + T+V F + A+++YK +L PP+
Sbjct: 258 AMGSVASGSTVVILRRFGLDDMMQAVEKYKATILSLAPPV 297
>At5g38120.1 68418.m04592 4-coumarate--CoA ligase family protein /
4-coumaroyl-CoA synthase family protein similar to 4CL2,
Arabidopsis thaliana [gi:12229665], 4CL1, Nicotiana
tabacum [gi:12229631]; contains Pfam AMP-binding enzyme
domain PF00501
Length = 550
Score = 62.1 bits (144), Expect = 1e-10
Identities = 30/100 (30%), Positives = 56/100 (56%), Gaps = 1/100 (1%)
Frame = +1
Query: 118 DTVFILFSSGTTGLPKGVMLTHINALCAAMNFENDNMHEDKFKTMLSIVPWFHAYGLIS- 294
DT +L+SSGTTG KGV +H N + + + + + +T + VP FH +GL++
Sbjct: 200 DTAMLLYSSGTTGRSKGVNSSHGNLIAHVARYIAEPFEQPQ-QTFICTVPLFHTFGLLNF 258
Query: 295 TINYILIKKTIVYFSGFNPQKYLNAIQEYKVNVLVAVPPI 414
+ + + T+V F+ + + A+++Y+ L+ VPP+
Sbjct: 259 VLATLALGTTVVILPRFDLGEMMAAVEKYRATTLILVPPV 298
>At1g62940.1 68414.m07107 4-coumarate--CoA ligase family protein /
4-coumaroyl-CoA synthase family protein similar to
gi:112801 from Petroselinum crispum, GB:AAD40664 from
[Solanum tuberosum] (J. Biol. Chem. 266 (13), 8551-8559
(1991)); contains Pfam AMP-binding enzyme domain PF00501
Length = 542
Score = 60.1 bits (139), Expect = 6e-10
Identities = 43/135 (31%), Positives = 66/135 (48%), Gaps = 4/135 (2%)
Frame = +1
Query: 22 GKPLESGIISYSDIKVETDVRSYEPAEVQGWTDTVFILFSSGTTGLPKGVMLTHINA--- 192
G+ G +++ D+ D E TD + FSSGTTGL KGVMLTH N
Sbjct: 150 GEEKIEGAVNWKDLLEAGDKCGDTDNEEILQTDLCALPFSSGTTGLQKGVMLTHRNLIAN 209
Query: 193 LCAAMNFENDNMHEDKFKTMLSIVPWFHAYGLISTINYILIKK-TIVYFSGFNPQKYLNA 369
LC+ + M L ++P+FH YG++ + K +V S ++ + +LNA
Sbjct: 210 LCSTLFGVRSEMIGQ--IVTLGLIPFFHIYGIVGICCATMKNKGKVVAMSRYDLRIFLNA 267
Query: 370 IQEYKVNVLVAVPPI 414
+ ++V+ VPPI
Sbjct: 268 LIAHEVSFAPIVPPI 282
>At4g19010.1 68417.m02802 4-coumarate--CoA ligase family protein /
4-coumaroyl-CoA synthase family protein similar to 4CL
from Pinus taeda, gi:515503, gi:1143308; contains Pfam
AMP-binding enzyme domain PF00501
Length = 566
Score = 57.2 bits (132), Expect = 4e-09
Identities = 33/104 (31%), Positives = 53/104 (50%), Gaps = 5/104 (4%)
Frame = +1
Query: 118 DTVFILFSSGTTGLPKGVMLTHINALCAA---MNFE-NDNMHEDKFKTMLSIVPWFHAYG 285
D I++SSGTTG KGV+LTH N + + + FE + + L+ +P H YG
Sbjct: 205 DVAAIMYSSGTTGASKGVLLTHRNLIASMELFVRFEASQYEYPGSSNVYLAALPLCHIYG 264
Query: 286 L-ISTINYILIKKTIVYFSGFNPQKYLNAIQEYKVNVLVAVPPI 414
L + + + + TIV F+ +N I+ +K+ VPP+
Sbjct: 265 LSLFVMGLLSLGSTIVVMKRFDASDVVNVIERFKITHFPVVPPM 308
>At4g14070.1 68417.m02172 AMP-binding protein, putative similar to
AMP-binding protein [gi:1617272] from Brassica napus;
contains Pfam AMP-binding enzyme domain PF00501;
identical to cDNA acyl-CoA synthetase-like protein
GI:20799730
Length = 727
Score = 54.0 bits (124), Expect = 4e-08
Identities = 43/129 (33%), Positives = 63/129 (48%), Gaps = 3/129 (2%)
Frame = +1
Query: 31 LESGIISYSDIKVETDVRSYEPAEVQGWTDTVFILFSSGTTGLPKGVMLTHINALCAAMN 210
+ G S + + D RSY + DT I+++SGTTG PKGVMLTH N L +
Sbjct: 252 INQGQESRAKLSASNDTRSYRNQFIDS-DDTAAIMYTSGTTGNPKGVMLTHRNLLHQIKH 310
Query: 211 FEN--DNMHEDKFKTMLSIVPWFHAYGLISTINYILIKKTIVYFSGFNPQKYL-NAIQEY 381
DKF LS++P +HAY S Y + T + +YL + ++ Y
Sbjct: 311 LSKYVPAQAGDKF---LSMLPSWHAYERAS--EYFIF--TCGVEQMYTSIRYLKDDLKRY 363
Query: 382 KVNVLVAVP 408
+ N +V+VP
Sbjct: 364 QPNYIVSVP 372
>At1g20480.1 68414.m02552 4-coumarate--CoA ligase family protein /
4-coumaroyl-CoA synthase family protein similar to
SP|Q9S725 from Arabidopsis thaliana and SP|P17814 from
Oryza sativa; contains Pfam AMP-binding enzyme domain
PF00501
Length = 565
Score = 54.0 bits (124), Expect = 4e-08
Identities = 35/125 (28%), Positives = 58/125 (46%), Gaps = 1/125 (0%)
Frame = +1
Query: 43 IISYSDIKVETDVRSYEPAEVQGWTDTVFILFSSGTTGLPKGVMLTHINALCAAMNFEND 222
++ + +ET+ + DT +L+SSGTTG KGVML+H N + +
Sbjct: 189 LVGRLETMIETEPSESRVKQRVNQDDTAALLYSSGTTGTSKGVMLSHRNLIALVQAYRAR 248
Query: 223 NMHEDKFKTMLSIVPWFHAYGLIS-TINYILIKKTIVYFSGFNPQKYLNAIQEYKVNVLV 399
E + + +P H +G I + TIV F+ K L+A++ ++ + L
Sbjct: 249 FGLEQR---TICTIPMCHIFGFGGFATGLIALGWTIVVLPKFDMAKLLSAVETHRSSYLS 305
Query: 400 AVPPI 414
VPPI
Sbjct: 306 LVPPI 310
>At3g23790.1 68416.m02990 AMP-binding protein, putative similar to
AMP-binding protein GB:CAA96521 from [Brassica napus]
(Plant Mol. Biol. (1997) 33 (5), 911-922); contains Pfam
AMP-binding enzyme domain PF00501; identical to cDNA
acyl-CoA synthetase-like protein GI:20799732
Length = 722
Score = 48.0 bits (109), Expect = 3e-06
Identities = 25/55 (45%), Positives = 35/55 (63%)
Frame = +1
Query: 118 DTVFILFSSGTTGLPKGVMLTHINALCAAMNFENDNMHEDKFKTMLSIVPWFHAY 282
D I+++SGTTG PKGVMLTH N L N +D + + + LS++P +HAY
Sbjct: 264 DIATIMYTSGTTGNPKGVMLTHQNLLHQIRNL-SDFVPAEAGERFLSMLPSWHAY 317
>At2g04350.2 68415.m00434 long-chain-fatty-acid--CoA ligase family
protein / long-chain acyl-CoA synthetase family protein
(LACS8) similar to LACS 4 [SP|O35547] from Rattus
norvegicus, LACS 4 [SP|O60488] from Homo sapiens;
contains Pfam HMM hit: AMP-binding enzymes PF00501
Length = 720
Score = 46.0 bits (104), Expect = 1e-05
Identities = 29/94 (30%), Positives = 44/94 (46%)
Frame = +1
Query: 130 ILFSSGTTGLPKGVMLTHINALCAAMNFENDNMHEDKFKTMLSIVPWFHAYGLISTINYI 309
I+F+SG+TGLPKGVM+TH N + A DK T ++ +P H + L + I
Sbjct: 279 IMFTSGSTGLPKGVMITHGNLVATAAGVMKVVPKLDKNDTYIAYLPLAHVFELEAEIVVF 338
Query: 310 LIKKTIVYFSGFNPQKYLNAIQEYKVNVLVAVPP 411
I Y S N +++ + A+ P
Sbjct: 339 TSGSAIGYGSAMTLTDTSNKVKKGTKGDVSALKP 372
>At2g04350.1 68415.m00433 long-chain-fatty-acid--CoA ligase family
protein / long-chain acyl-CoA synthetase family protein
(LACS8) similar to LACS 4 [SP|O35547] from Rattus
norvegicus, LACS 4 [SP|O60488] from Homo sapiens;
contains Pfam HMM hit: AMP-binding enzymes PF00501
Length = 720
Score = 46.0 bits (104), Expect = 1e-05
Identities = 29/94 (30%), Positives = 44/94 (46%)
Frame = +1
Query: 130 ILFSSGTTGLPKGVMLTHINALCAAMNFENDNMHEDKFKTMLSIVPWFHAYGLISTINYI 309
I+F+SG+TGLPKGVM+TH N + A DK T ++ +P H + L + I
Sbjct: 279 IMFTSGSTGLPKGVMITHGNLVATAAGVMKVVPKLDKNDTYIAYLPLAHVFELEAEIVVF 338
Query: 310 LIKKTIVYFSGFNPQKYLNAIQEYKVNVLVAVPP 411
I Y S N +++ + A+ P
Sbjct: 339 TSGSAIGYGSAMTLTDTSNKVKKGTKGDVSALKP 372
>At3g05970.1 68416.m00681 long-chain-fatty-acid--CoA ligase /
long-chain acyl-CoA synthetase (LACS6) strong similarity
to AMP-binding protein (MF39P) gi:1617274 from Brassica
napus, similar to putative long-chain-fatty-acid--CoA
ligase (brain isozyme) GB:P33124 [Rattus norvegicus];
contains Pfam AMP-binding enzyme domain PF00501;
identical to cDNA AtLACS6 for long-chain acyl-CoA
synthetase GI:22531705
Length = 701
Score = 45.2 bits (102), Expect = 2e-05
Identities = 30/99 (30%), Positives = 47/99 (47%), Gaps = 2/99 (2%)
Frame = +1
Query: 118 DTVFILFSSGTTGLPKGVMLTHINALC--AAMNFENDNMHEDKFKTMLSIVPWFHAYGLI 291
D I ++SGTTG PKGV+LTH N + A +F D + +S +P H Y
Sbjct: 262 DVATICYTSGTTGTPKGVVLTHANLIANVAGSSFSVKFFSSDVY---ISYLPLAHIYERA 318
Query: 292 STINYILIKKTIVYFSGFNPQKYLNAIQEYKVNVLVAVP 408
+ I + + ++ G N K L+ + + V +VP
Sbjct: 319 NQILTVYFGVAVGFYQGDN-MKLLDDLAALRPTVFSSVP 356
>At1g30520.1 68414.m03734 acyl-activating enzyme 14 (AAE14)
identical to acyl-activating enzyme 14 [Arabidopsis
thaliana]; similar to SP|Q42524 4-coumarate--CoA ligase
1 (EC 6.2.1.12) (4-coumaroyl-CoA synthase 1)
{Arabidopsis thaliana}; contains Pfam profile PF00501:
AMP-binding enzyme; identical to cDNA acyl-activating
enzyme 14 (At1g30520) GI:29893263
Length = 560
Score = 42.7 bits (96), Expect = 1e-04
Identities = 27/98 (27%), Positives = 45/98 (45%), Gaps = 1/98 (1%)
Frame = +1
Query: 118 DTVFILFSSGTTGLPKGVMLTHINALCAAM-NFENDNMHEDKFKTMLSIVPWFHAYGLIS 294
D V I F+SGTTG PKGV ++H+ + ++ ED L P H GL S
Sbjct: 173 DAVVICFTSGTTGRPKGVTISHLAFITQSLAKIAIAGYGED--DVYLHTSPLVHIGGLSS 230
Query: 295 TINYILIKKTIVYFSGFNPQKYLNAIQEYKVNVLVAVP 408
+ +++ V F+ + L +++ + + VP
Sbjct: 231 AMAMLMVGACHVLLPKFDAKTALQVMEQNHITCFITVP 268
>At1g49430.1 68414.m05541 long-chain-fatty-acid--CoA ligase /
long-chain acyl-CoA synthetase nearly identical to acyl
CoA synthetase (MF45P) GI:1617268 from [Brassica napus]
Length = 665
Score = 42.3 bits (95), Expect = 1e-04
Identities = 31/106 (29%), Positives = 49/106 (46%), Gaps = 6/106 (5%)
Frame = +1
Query: 115 TDTVFILFSSGTTGLPKGVMLTHINALCAAMNFENDNMHE------DKFKTMLSIVPWFH 276
TD I+++SGTTG PKGV+L NA + D M E D S +P H
Sbjct: 223 TDICTIMYTSGTTGEPKGVILN--NAAISVQVLSIDKMLEVTDRSCDTSDVFFSYLPLAH 280
Query: 277 AYGLISTINYILIKKTIVYFSGFNPQKYLNAIQEYKVNVLVAVPPI 414
Y + I ++ ++ Y+ G + + ++ +Q K V VP +
Sbjct: 281 CYDQVMEIYFLSRGSSVGYWRG-DIRYLMDDVQALKPTVFCGVPRV 325
>At3g16170.1 68416.m02041 acyl-activating enzyme 13 (AAE13) similar
to malonyl CoA synthetase GB:AAF28840 from
[Bradyrhizobium japonicum]; contains Pfam AMP-binding
enzyme domain PF00501; identical to cDNA acyl-activating
enzyme 13 (At3g16170) GI:29893232, acyl-activating
enzyme 13 [Arabidopsis thaliana] GI:29893233
Length = 544
Score = 41.9 bits (94), Expect = 2e-04
Identities = 26/84 (30%), Positives = 44/84 (52%)
Frame = +1
Query: 85 SYEPAEVQGWTDTVFILFSSGTTGLPKGVMLTHINALCAAMNFENDNMHEDKFKTMLSIV 264
S+E AE + D I+++SGTTG PKGV+ TH N++ + + + L +
Sbjct: 161 SFE-AEGKFLDDPALIVYTSGTTGKPKGVVHTH-NSINSQVRMLTEAWEYTSADHFLHCL 218
Query: 265 PWFHAYGLISTINYILIKKTIVYF 336
P H +GL + + L +++V F
Sbjct: 219 PLHHVHGLFNALFAPLYARSLVEF 242
>At3g48990.1 68416.m05351 AMP-dependent synthetase and ligase family
protein similar to peroxisomal-coenzyme A synthetase
(FAT2) [gi:586339] from Saccharomyces cerevisiae;
contains Pfam AMP-binding enzyme domain PF00501;
identical to cDNA; identical to cDNA adenosine
monophosphate binding protein 3 AMPBP3
(AMPBP3)GI:20799714
Length = 514
Score = 40.7 bits (91), Expect = 4e-04
Identities = 28/102 (27%), Positives = 47/102 (46%), Gaps = 3/102 (2%)
Frame = +1
Query: 118 DTVFILFSSGTTGLPKGVMLTHINALCAAMNFENDNMHEDKFKTMLSIVPWFHAYGLIST 297
D L +SGTT PKGV LT +N + N + + T++ ++P FH +GL++
Sbjct: 163 DGALFLHTSGTTSRPKGVPLTQLNLASSVKNIKAVYKLTESDSTVI-VLPLFHVHGLLAG 221
Query: 298 INYIL---IKKTIVYFSGFNPQKYLNAIQEYKVNVLVAVPPI 414
+ L T+ F+ + +++Y AVP I
Sbjct: 222 LLSSLGAGAAVTLPAAGRFSATTFWPDMKKYNATWYTAVPTI 263
>At2g47240.1 68415.m05899 long-chain-fatty-acid--CoA ligase family
protein / long-chain acyl-CoA synthetase family protein
similar to GI:1617270 (MF7P) and gi:1617628 (MF45P) from
[Brassica napus] ; contains Pfam AMP-binding enzyme
domain PF00501
Length = 660
Score = 40.7 bits (91), Expect = 4e-04
Identities = 31/102 (30%), Positives = 50/102 (49%), Gaps = 7/102 (6%)
Frame = +1
Query: 130 ILFSSGTTGLPKGVMLTH--INALCAAMN-----FENDNMHEDKFKTMLSIVPWFHAYGL 288
I+++SGT+G PKGV+LTH + M+ FE+ H+D + LS +P H
Sbjct: 225 IMYTSGTSGDPKGVVLTHQAVATFVVGMDLYMDQFEDKMTHDDVY---LSFLPLAHILDR 281
Query: 289 ISTINYILIKKTIVYFSGFNPQKYLNAIQEYKVNVLVAVPPI 414
++ + ++ Y+ G N + IQE K L VP +
Sbjct: 282 MNEEYFFRKGASVGYYHG-NLNVLRDDIQELKPTYLAGVPRV 322
>At3g16910.1 68416.m02162 AMP-dependent synthetase and ligase family
protein similar to AMP-binding protein GI:1903034 from
[Brassica napus]; contains Pfam AMP-binding domain
PF00501; identical to cDNA adenosine monophosphate
binding protein 7 AMPBP7 (AMPBP7) GI:20799722
Length = 569
Score = 40.3 bits (90), Expect = 5e-04
Identities = 32/130 (24%), Positives = 52/130 (40%)
Frame = +1
Query: 25 KPLESGIISYSDIKVETDVRSYEPAEVQGWTDTVFILFSSGTTGLPKGVMLTHINALCAA 204
+ L G I Y D D W ++ + ++SGTT PKGV+L H A A
Sbjct: 168 RALSKGAIEYEDFLATGDPNYPWQPPADEW-QSIALGYTSGTTASPKGVVLHHRGAYIMA 226
Query: 205 MNFENDNMHEDKFKTMLSIVPWFHAYGLISTINYILIKKTIVYFSGFNPQKYLNAIQEYK 384
++ +D L +P FH G + ++ T + ++ + I +YK
Sbjct: 227 LSNPLIWGMQDG-AVYLWTLPMFHCNGWCFPWSLAVLSGTSICLRQVTAKEVYSMIAKYK 285
Query: 385 VNVLVAVPPI 414
V A P +
Sbjct: 286 VTHFCAAPVV 295
>At1g77590.1 68414.m09034 long-chain-fatty-acid--CoA ligase family
protein / long-chain acyl-CoA synthetase family protein
(LACS9) similar to LACS 3 [SP|O95573] from Homo Sapiens,
LACS 3 [SP|Q63151] from Rattus norvegicus; contains Pfam
HMM hit: AMP-binding enzymes PF00501
Length = 691
Score = 39.9 bits (89), Expect = 7e-04
Identities = 16/26 (61%), Positives = 21/26 (80%)
Frame = +1
Query: 118 DTVFILFSSGTTGLPKGVMLTHINAL 195
D I+++SG+TGLPKGVM+TH N L
Sbjct: 246 DVAVIMYTSGSTGLPKGVMMTHGNVL 271
>At5g27600.1 68418.m03305 AMP-binding protein, putative similar to
AMP-binding protein (MF39P) gi:1617274 from Brassica
napus, long-chain-fatty-acid--CoA ligase - Brassica
napus, EMBL:Z72152; contains Pfam AMP-binding enzyme
domain PF00501
Length = 700
Score = 37.9 bits (84), Expect = 0.003
Identities = 27/82 (32%), Positives = 39/82 (47%), Gaps = 2/82 (2%)
Frame = +1
Query: 43 IISYSDIKVETDVRSYEPAEVQGWTDTVFILFSSGTTGLPKGVMLTHINALC--AAMNFE 216
I+SY + + S P D I ++SGTTG PKGV+LTH N + A + E
Sbjct: 238 IVSYQKL-LSQGRSSLHPFSPPKPEDIATICYTSGTTGTPKGVVLTHGNLIANVAGSSVE 296
Query: 217 NDNMHEDKFKTMLSIVPWFHAY 282
+ D + +S +P H Y
Sbjct: 297 AEFFPSDVY---ISYLPLAHIY 315
>At1g77240.1 68414.m08996 AMP-binding protein, putative strong
similarity to AMP-binding protein GI:1903034 from
[Brassica napus]; contains Pfam AMP-binding domain
PF00501
Length = 545
Score = 37.1 bits (82), Expect = 0.005
Identities = 27/101 (26%), Positives = 46/101 (45%)
Frame = +1
Query: 112 WTDTVFILFSSGTTGLPKGVMLTHINALCAAMNFENDNMHEDKFKTMLSIVPWFHAYGLI 291
WT V + ++SGTT PKGV+ +H + + +N D ++ L +P FHA G
Sbjct: 185 WTPMV-LNYTSGTTSSPKGVVHSHRSVFMSTINSLLDWSLPNR-PVYLWTLPMFHANGWS 242
Query: 292 STINYILIKKTIVYFSGFNPQKYLNAIQEYKVNVLVAVPPI 414
T + + + + N I +Y+V + A P +
Sbjct: 243 YTWATAAVGARNICVTRVDVPTIFNLIDKYQVTHMCAAPMV 283
>At1g20560.1 68414.m02563 AMP-dependent synthetase and ligase family
protein similar to AMP-binding protein GI:1903034 from
[Brassica napus]; contains Pfam AMP-binding domain
PF00501; identical to adenosine monophosphate binding
protein 1 AMPBP1 (AMPBP1) GI:20799710
Length = 556
Score = 36.7 bits (81), Expect = 0.006
Identities = 33/128 (25%), Positives = 52/128 (40%), Gaps = 4/128 (3%)
Frame = +1
Query: 43 IISYSDIKVETDVRSYEPAEVQGWTDTVFILFSSGTTGLPKGVMLTH----INALCAAMN 210
++ Y D+ V +E D + + ++SGTT PKGV+ +H +N+L A +
Sbjct: 164 MMEYEDV-VAMGKSDFEVIRPTDECDAISVNYTSGTTSSPKGVVYSHRGAYLNSLAAVLL 222
Query: 211 FENDNMHEDKFKTMLSIVPWFHAYGLISTINYILIKKTIVYFSGFNPQKYLNAIQEYKVN 390
E MH T L P FH G I T + + + I ++KV
Sbjct: 223 NE---MHSS--PTYLWTNPMFHCNGWCLLWGVTAIGGTNICLRNVTAKAIFDNISQHKVT 277
Query: 391 VLVAVPPI 414
+ P I
Sbjct: 278 HMGGAPTI 285
>At4g11030.1 68417.m01794 long-chain-fatty-acid--CoA ligase,
putative / long-chain acyl-CoA synthetase, putative
similar to acyl-CoA synthetase (MF7P) gi:1617270 from
Brassica napus
Length = 666
Score = 36.3 bits (80), Expect = 0.008
Identities = 27/104 (25%), Positives = 51/104 (49%), Gaps = 4/104 (3%)
Frame = +1
Query: 115 TDTVFILFSSGTTGLPKGVMLTH---INALCAAMNF-ENDNMHEDKFKTMLSIVPWFHAY 282
+D I+++SGTTG PKGVM+++ + M+F N N + +S +P H +
Sbjct: 223 SDICTIMYTSGTTGDPKGVMISNESIVTITTGVMHFLGNVNASLSEKDVYISYLPLAHVF 282
Query: 283 GLISTINYILIKKTIVYFSGFNPQKYLNAIQEYKVNVLVAVPPI 414
I + +I ++ G + + + + E K ++ AVP +
Sbjct: 283 DRAIEECIIQVGGSIGFWRG-DVKLLIEDLGELKPSIFCAVPRV 325
>At5g36880.1 68418.m04418 acetyl-CoA synthetase, putative /
acetate-CoA ligase, putative similar to SP|P27550
(Escherichia coli) and gi:8439651 (Homo sapiens);
contains Pfam AMP-binding enzyme domain PF00501
Length = 693
Score = 35.5 bits (78), Expect = 0.014
Identities = 26/106 (24%), Positives = 49/106 (46%), Gaps = 7/106 (6%)
Frame = +1
Query: 118 DTVFILFSSGTTGLPKGVMLT---HINALCAAMNFENDNMHEDKFKTMLSIVPWFHAYGL 288
D +F+L++SG+TG PKGV+ T ++ + D D + + W +
Sbjct: 303 DPLFLLYTSGSTGKPKGVLHTTGGYMIYTATTFKYAFDYKSTDVY-WCTADCGWITGHSY 361
Query: 289 ISTINYILIKKTIVYFSGF----NPQKYLNAIQEYKVNVLVAVPPI 414
+ T +L T+V F G +P + + + +YKV++ P +
Sbjct: 362 V-TYGPMLNGATVVVFEGAPNYPDPGRCWDIVDKYKVSIFYTAPTL 406
>At1g66120.1 68414.m07504 acyl-activating enzyme 11 (AAE11) similar
to AMP-binding protein GI:1903034 from [Brassica napus];
contains Pfam AMP-binding domain PF00501; identical to
cDNA acyl-activating enzyme 11 (At1g66120) GI:29893230,
acyl-activating enzyme 11 [Arabidopsis thaliana]
GI:29893231
Length = 572
Score = 33.1 bits (72), Expect = 0.077
Identities = 19/60 (31%), Positives = 30/60 (50%)
Frame = +1
Query: 118 DTVFILFSSGTTGLPKGVMLTHINALCAAMNFENDNMHEDKFKTMLSIVPWFHAYGLIST 297
D + + ++SGTT PKGV+++H A +A++ F L +P FH G T
Sbjct: 187 DPISLNYTSGTTADPKGVVISHQGAYLSALS-SIIGWEMGIFPVYLWTLPMFHCNGWTHT 245
>At2g17650.1 68415.m02042 AMP-dependent synthetase and ligase family
protein similar to AMP-binding protein GI:1903034 from
[Brassica napus]; contains Pfam AMP-binding domain
PF00501; identical to cDNA adenosine monophosphate
binding protein 2 AMPBP2 (AMPBP2) GI:20799712
Length = 603
Score = 32.7 bits (71), Expect = 0.10
Identities = 25/100 (25%), Positives = 41/100 (41%), Gaps = 1/100 (1%)
Frame = +1
Query: 118 DTVFILFSSGTTGLPKGVMLTHINALCAAMNFENDNMHE-DKFKTMLSIVPWFHAYGLIS 294
D + I ++SGTT PKGV+ +H A ++ +H+ + L VP FH G
Sbjct: 242 DPISINYTSGTTSRPKGVVYSHRGAYLNSL--ATVFLHQMSVYPVYLWTVPMFHCNGWCL 299
Query: 295 TINYILIKKTIVYFSGFNPQKYLNAIQEYKVNVLVAVPPI 414
T + +P+ I +KV + P +
Sbjct: 300 VWGVAAQGGTNICLRKVSPKMIFKNIAMHKVTHMGGAPTV 339
>At4g23850.1 68417.m03429 long-chain-fatty-acid--CoA ligase /
long-chain acyl-CoA synthetase nearly identical to
acyl-CoA synthetase (MF7P) from Brassica napus
[gi:1617270]
Length = 666
Score = 32.3 bits (70), Expect = 0.14
Identities = 28/104 (26%), Positives = 50/104 (48%), Gaps = 4/104 (3%)
Frame = +1
Query: 115 TDTVFILFSSGTTGLPKGVMLTH--INALCAAM--NFENDNMHEDKFKTMLSIVPWFHAY 282
+D I+++SGTTG PKGVM+++ I L A + ++ N LS +P H +
Sbjct: 223 SDICTIMYTSGTTGDPKGVMISNESIVTLIAGVIRLLKSANEALTVKDVYLSYLPLAHIF 282
Query: 283 GLISTINYILIKKTIVYFSGFNPQKYLNAIQEYKVNVLVAVPPI 414
+ +I I ++ G + + + + E K + AVP +
Sbjct: 283 DRVIEECFIQHGAAIGFWRG-DVKLLIEDLAELKPTIFCAVPRV 325
>At1g64400.1 68414.m07299 long-chain-fatty-acid--CoA ligase,
putative / long-chain acyl-CoA synthetase, putative
similar to GI:1617270 (MF7P) from [Brassica napus]
Length = 665
Score = 32.3 bits (70), Expect = 0.14
Identities = 13/23 (56%), Positives = 19/23 (82%)
Frame = +1
Query: 115 TDTVFILFSSGTTGLPKGVMLTH 183
+D I+++SGTTG PKGV+LT+
Sbjct: 223 SDVCTIMYTSGTTGDPKGVLLTN 245
>At1g21540.1 68414.m02694 AMP-binding protein, putative strong
similarity to AMP-binding protein GI:1903034 from
[Brassica napus]; contains Pfam AMP-binding domain
PF00501; identical to cDNA adenosine monophosphate
binding protein 9 AMPBP9 (AMPBP9) GI:20799726
Length = 550
Score = 32.3 bits (70), Expect = 0.14
Identities = 25/93 (26%), Positives = 39/93 (41%)
Frame = +1
Query: 136 FSSGTTGLPKGVMLTHINALCAAMNFENDNMHEDKFKTMLSIVPWFHAYGLISTINYILI 315
++SGTT PKGV+L+H ++ D H L +P FHA G T +
Sbjct: 194 YTSGTTSSPKGVVLSHRAIFMLTVSSLLD-WHFPNRPVYLWTLPMFHANGWGYTWGTAAV 252
Query: 316 KKTIVYFSGFNPQKYLNAIQEYKVNVLVAVPPI 414
T V + + I ++ V + A P +
Sbjct: 253 GATNVCTRRVDAPTIYDLIDKHHVTHMCAAPMV 285
>At1g21530.1 68414.m02693 AMP-binding protein, putative strong
similarity to AMP-binding protein GI:1903034 from
[Brassica napus]; contains Pfam AMP-binding domain
PF00501; identical to cDNA adenosine monophosphate
binding protein 10 AMPBP10 (AMPBP10) GI:20799728
Length = 547
Score = 31.9 bits (69), Expect = 0.18
Identities = 24/93 (25%), Positives = 40/93 (43%)
Frame = +1
Query: 136 FSSGTTGLPKGVMLTHINALCAAMNFENDNMHEDKFKTMLSIVPWFHAYGLISTINYILI 315
++SGTT PKGV+L+H ++ D ++ L +P FHA G T +
Sbjct: 191 YTSGTTSSPKGVVLSHRAIFMLTVSSLLDWSVPNR-PVYLWTLPMFHANGWGYTWGTAAV 249
Query: 316 KKTIVYFSGFNPQKYLNAIQEYKVNVLVAVPPI 414
T + + N I ++ V + A P +
Sbjct: 250 GATNICTRRVDAPTIYNLIDKHNVTHMCAAPMV 282
>At5g23050.1 68418.m02695 acyl-activating enzyme 17 (AAE17) nearly
identical to acyl-activating enzyme 17 [Arabidopsis
thaliana] GI:29893266; similar to acetyl-CoA synthetase
[SP|P27095] from Methanothrix soehngenii; contains Pfam
AMP-binding enzyme domain PF00501; identical to cDNA
acyl-activating enzyme 17 (At5g23050) GI:29893265
Length = 721
Score = 31.5 bits (68), Expect = 0.24
Identities = 15/25 (60%), Positives = 19/25 (76%)
Frame = +1
Query: 130 ILFSSGTTGLPKGVMLTHINALCAA 204
ILFSSGTTG PK + T+I+ L +A
Sbjct: 354 ILFSSGTTGEPKAIPWTNISPLKSA 378
>At5g16340.1 68418.m01910 AMP-binding protein, putative similar to
AMP-binding protein GI:1903034 from [Brassica napus];
contains Pfam AMP-binding domain PF00501; identical to
adenosine monophosphate binding protein 6 AMPBP6
(AMPBP6) GI:20799720
Length = 550
Score = 31.1 bits (67), Expect = 0.31
Identities = 24/99 (24%), Positives = 41/99 (41%)
Frame = +1
Query: 118 DTVFILFSSGTTGLPKGVMLTHINALCAAMNFENDNMHEDKFKTMLSIVPWFHAYGLIST 297
D V + ++SGTT PKGV+ H +++ D K L +P FH+ G +
Sbjct: 186 DPVVLNYTSGTTSAPKGVVHCHRGIFVMSVDSLID-WAVPKNPVYLWTLPIFHSNGWTNP 244
Query: 298 INYILIKKTIVYFSGFNPQKYLNAIQEYKVNVLVAVPPI 414
+ T V F+ I+++ V + P +
Sbjct: 245 WGIAAVGGTNVCLRKFDAPLIYRLIRDHGVTHMCGAPVV 283
>At1g65880.1 68414.m07476 AMP-dependent synthetase and ligase family
protein similar to AMP-binding protein GI:1903034 from
[Brassica napus]; contains Pfam AMP-binding domain
PF00501
Length = 580
Score = 31.1 bits (67), Expect = 0.31
Identities = 27/105 (25%), Positives = 41/105 (39%), Gaps = 1/105 (0%)
Frame = +1
Query: 103 VQGWTDTVFILFSSGTTGLPKGVMLTHINA-LCAAMNFENDNMHEDKFKTMLSIVPWFHA 279
+Q D + + ++SGTT PKGV+++H A LC M L +P FH
Sbjct: 182 IQDEHDPISLNYTSGTTADPKGVVISHRGAYLCTLSAIIGWEM--GTCPVYLWTLPMFHC 239
Query: 280 YGLISTINYILIKKTIVYFSGFNPQKYLNAIQEYKVNVLVAVPPI 414
G T T V + I+ + V + VP +
Sbjct: 240 NGWTFTWGTAARGGTSVCMRHVTAPEIYKNIEMHNVTHMCCVPTV 284
>At5g16370.1 68418.m01913 AMP-binding protein, putative similar to
AMP-binding protein GI:1903034 from [Brassica napus];
contains Pfam AMP-binding domain PF00501; identical to
cDNA adenosine monophosphate binding protein 5 AMPBP5
(AMPBP5) GI:20799718
Length = 552
Score = 30.7 bits (66), Expect = 0.41
Identities = 25/99 (25%), Positives = 40/99 (40%)
Frame = +1
Query: 118 DTVFILFSSGTTGLPKGVMLTHINALCAAMNFENDNMHEDKFKTMLSIVPWFHAYGLIST 297
D V + ++SGTT PKGV+ H +++ D K L +P FHA G
Sbjct: 186 DPVVLNYTSGTTSAPKGVVHCHRGIFVMSIDSLID-WTVPKNPVYLWTLPIFHANGWSYP 244
Query: 298 INYILIKKTIVYFSGFNPQKYLNAIQEYKVNVLVAVPPI 414
+ T V F+ I+++ V + P +
Sbjct: 245 WGIAAVGGTNVCLRKFDAPLIYRLIRDHGVTHMCGAPVV 283
>At1g75960.1 68414.m08822 AMP-binding protein, putative similar to
AMP-binding protein GI:1903034 from [Brassica napus];
contains Pfam profile: PF00501 AMP-binding enzyme;
identical to cDNA adenosine monophosphate binding
protein 8 AMPBP8 (AMPBP8) GI:20799724
Length = 544
Score = 30.3 bits (65), Expect = 0.54
Identities = 18/56 (32%), Positives = 26/56 (46%)
Frame = +1
Query: 118 DTVFILFSSGTTGLPKGVMLTHINALCAAMNFENDNMHEDKFKTMLSIVPWFHAYG 285
D + + ++SGTT PKGV+ H ++ D K L +P FHA G
Sbjct: 185 DPIVVNYTSGTTSSPKGVVHCHRGIFVMTLDSLTD-WAVPKTPVYLWTLPIFHANG 239
>At1g68270.1 68414.m07798 AMP-dependent synthetase and ligase family
protein similar to AMP-binding protein GI:1903034 from
[Brassica napus]; contains Pfam AMP-binding domain
PF00501
Length = 535
Score = 30.3 bits (65), Expect = 0.54
Identities = 12/35 (34%), Positives = 22/35 (62%)
Frame = +1
Query: 103 VQGWTDTVFILFSSGTTGLPKGVMLTHINALCAAM 207
+Q D + + ++SGTT PKGV+++H A ++
Sbjct: 182 IQNEQDPISLNYTSGTTADPKGVVISHRGAYLTSL 216
>At1g65890.1 68414.m07477 acyl-activating enzyme 12 (AAE12) similar
to AMP-binding protein GI:1903034 from [Brassica napus];
contains Pfam AMP-binding domain PF00501; identical to
cDNA acyl-activating enzyme 12 (At1g65890) mRNA
GI:29893228, acyl-activating enzyme 12 [Arabidopsis
thaliana] GI:29893229
Length = 578
Score = 30.3 bits (65), Expect = 0.54
Identities = 24/104 (23%), Positives = 41/104 (39%)
Frame = +1
Query: 103 VQGWTDTVFILFSSGTTGLPKGVMLTHINALCAAMNFENDNMHEDKFKTMLSIVPWFHAY 282
+Q D + + ++SGTT PKGV+++H A + ++ L +P FH
Sbjct: 182 IQDEHDPISLNYTSGTTADPKGVVISHRGAYLSTLS-AIIGWEMGTCPVYLWTLPMFHCN 240
Query: 283 GLISTINYILIKKTIVYFSGFNPQKYLNAIQEYKVNVLVAVPPI 414
G T T V + I+ + V + VP +
Sbjct: 241 GWTFTWGTAARGGTSVCMRHVTAPEIYKNIEMHNVTHMCCVPTV 284
>At5g35930.1 68418.m04322 AMP-dependent synthetase and ligase family
protein similar to iturin A synthetase C [Bacillus
subtilis] GI:16040972; contains Pfam profile PF00501:
AMP-binding enzyme
Length = 1040
Score = 29.9 bits (64), Expect = 0.72
Identities = 29/122 (23%), Positives = 57/122 (46%), Gaps = 6/122 (4%)
Frame = +1
Query: 61 IKVETDVRSYE-PAEVQGWTDTVFILFSSGTTGLPKGVMLTHINALCAAMNFEN--DNMH 231
+ VET S+ P + + +++++SG+TG PKGV T L + + +
Sbjct: 83 LSVETGCSSFVWPCKKERQRKFCYLMYTSGSTGKPKGVCGTEQGLLNRFLWMQELYPVVG 142
Query: 232 EDK--FKTMLSIVPWFHAY-GLISTINYILIKKTIVYFSGFNPQKYLNAIQEYKVNVLVA 402
E + FKT + + + G I + ++I + N ++ ++EY ++ L+A
Sbjct: 143 EQRFAFKTSVGFIDHIQEFLGAILSSTALVIPPFTLLKE--NMISIIDFLEEYSISRLLA 200
Query: 403 VP 408
VP
Sbjct: 201 VP 202
>At1g55320.1 68414.m06319 acyl-activating enzyme 18 (AAE18) nearly
identical to acyl-activating enzyme 18 [Arabidopsis
thaliana] GI:29893268; similar to acetyl-CoA synthetase
[SP|P27095] from Methanothrix soehngenii; contains Pfam
AMP-binding enzyme domain PF00501l; identical to cDNA
acyl-activating enzyme 18 (At1g55320) GI: 29893267
Length = 725
Score = 29.5 bits (63), Expect = 0.95
Identities = 13/25 (52%), Positives = 18/25 (72%)
Frame = +1
Query: 130 ILFSSGTTGLPKGVMLTHINALCAA 204
ILFSSGTTG PK + T ++ + +A
Sbjct: 359 ILFSSGTTGEPKAIPWTQLSPIRSA 383
>At5g14880.1 68418.m01745 potassium transporter, putative similar to
potassium transporter HAK2p [Mesembryanthemum
crystallinum] gi|14091471|gb|AAK53759; KUP/HAK/KT
Transporter family member, PMID:11500563; contains Pfam
profile PF02705: K+ potassium transporter
Length = 781
Score = 27.1 bits (57), Expect = 5.1
Identities = 17/52 (32%), Positives = 25/52 (48%), Gaps = 4/52 (7%)
Frame = -3
Query: 315 YQYI---VDCRYQAVCMKPWYYGQHSLEFVFVHVIIFKIHC-STQSVYMC*H 172
+QY+ V C + +YG H L FVF +++ + C ST VY H
Sbjct: 194 HQYVEVPVVCAILILLFSLQHYGTHRLGFVFAPIVLAWLLCISTIGVYNIFH 245
>At5g49530.1 68418.m06130 SIN-like family protein low similarity to
Sex-lethal interactor [Drosophila melanogaster]
GI:6049274; contains Pfam profile PF04801: Sin-like
protein conserved region
Length = 689
Score = 26.6 bits (56), Expect = 6.7
Identities = 19/70 (27%), Positives = 33/70 (47%), Gaps = 2/70 (2%)
Frame = +1
Query: 178 THINALCAAMNFENDNMHEDKFKTMLSIVPWFHAYGLISTINYILIKKTIV--YFSGFNP 351
T+IN LC + N E + +LS+ P + LI+ +++ Y F+
Sbjct: 311 TYINELCRGGSSRNSESKETLKRVLLSL-PLKERVQKLLCEGSPLIRYSVLKHYAPEFSD 369
Query: 352 QKYLNAIQEY 381
+ +L A+QEY
Sbjct: 370 EDFLGALQEY 379
>At1g21410.1 68414.m02679 F-box family protein similar to SKP1
interacting partner 2 (SKIP2) TIGR_Ath1:At5g67250
Length = 360
Score = 26.2 bits (55), Expect = 8.9
Identities = 17/40 (42%), Positives = 18/40 (45%)
Frame = -1
Query: 299 IVDIKPYA*NHGTMDSIVLNLSSCMLSFSKFIAAHRAFIC 180
I D YA HG D LNLS C SFS A+ C
Sbjct: 130 ITDRSLYALAHGCPDLTKLNLSGC-TSFSDTAIAYLTRFC 168
>At1g20720.1 68414.m02596 helicase-related similar to BRCA1-binding
helicase-like protein BACH1 (GI:13661819) Homo
sapiens].;
Length = 1169
Score = 26.2 bits (55), Expect = 8.9
Identities = 12/33 (36%), Positives = 17/33 (51%)
Frame = -1
Query: 206 IAAHRAFICVSITPLGNPVVPDENNMNTVSVHP 108
+ A R C + LG V DE N+N ++ HP
Sbjct: 179 VLASRKHYCTNRHVLGKDNVDDEWNVNKITSHP 211
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 8,831,959
Number of Sequences: 28952
Number of extensions: 168248
Number of successful extensions: 437
Number of sequences better than 10.0: 52
Number of HSP's better than 10.0 without gapping: 413
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 418
length of database: 12,070,560
effective HSP length: 74
effective length of database: 9,928,112
effective search space used: 635399168
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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