Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= S06A01NCLL0002_C10
(465 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g26720.1 68416.m03341 glycosyl hydrolase family 38 protein si... 43 1e-04
At5g13980.2 68418.m01635 glycosyl hydrolase family 38 protein si... 42 2e-04
At5g13980.1 68418.m01634 glycosyl hydrolase family 38 protein si... 42 2e-04
At5g66150.1 68418.m08333 glycosyl hydrolase family 38 protein si... 37 0.006
At1g20720.1 68414.m02596 helicase-related similar to BRCA1-bindi... 29 2.0
At1g28695.1 68414.m03534 expressed protein ; expression supporte... 27 4.7
At2g21630.1 68415.m02573 transport protein, putative similar to ... 27 6.2
At3g01670.1 68416.m00098 expressed protein 27 8.3
>At3g26720.1 68416.m03341 glycosyl hydrolase family 38 protein
similar to lysosomal alpha-mannosidase GI:3522867 from
[Homo sapiens]
Length = 1019
Score = 42.7 bits (96), Expect = 1e-04
Identities = 29/127 (22%), Positives = 55/127 (43%), Gaps = 11/127 (8%)
Frame = +1
Query: 10 SQQAFNKMKQGKSSKQ-PLFNYDRCHFNESSCLTAETSDNFIVTIYNSLAWKVKKFIEIP 186
++ + K G +Q PL N C +E+ L+ ++ +V +YNSL WK ++ + +P
Sbjct: 450 AKSSTEKKNPGTKFQQCPLLNISYCPASEARLLSGKS---LVVVVYNSLGWKREEVVRVP 506
Query: 187 VVKADYEVYDPKGNKLPSNLAEIPDFLKHIPTR----------KSFATHILYFLTELSPS 336
V + V D G ++ L + + I + A H+L F + P
Sbjct: 507 VSSENVIVKDASGKEVVFQLLPLSEIALRIRNEYVKAYLGRSPRDTAKHVLAFTASVPPL 566
Query: 337 SMTSFYV 357
+S+ +
Sbjct: 567 GFSSYVI 573
>At5g13980.2 68418.m01635 glycosyl hydrolase family 38 protein
similar to alpha-mannosidase GI:1419374 from [Homo
sapiens]
Length = 1024
Score = 41.9 bits (94), Expect = 2e-04
Identities = 25/88 (28%), Positives = 45/88 (51%)
Frame = +1
Query: 94 SSCLTAETSDNFIVTIYNSLAWKVKKFIEIPVVKADYEVYDPKGNKLPSNLAEIPDFLKH 273
SS + + IV YN L WK + +PVV D V+D +G+++ S L D ++
Sbjct: 471 SSEVNLSDGKSLIVLAYNPLGWKRVDIVRLPVVGGDVSVHDSEGHEVESQLVPFTD--EY 528
Query: 274 IPTRKSFATHILYFLTELSPSSMTSFYV 357
+ RK H+ +L + SP+ + +++
Sbjct: 529 VALRK---YHVEAYLGQ-SPTQVPKYWL 552
>At5g13980.1 68418.m01634 glycosyl hydrolase family 38 protein
similar to alpha-mannosidase GI:1419374 from [Homo
sapiens]
Length = 921
Score = 41.9 bits (94), Expect = 2e-04
Identities = 25/88 (28%), Positives = 45/88 (51%)
Frame = +1
Query: 94 SSCLTAETSDNFIVTIYNSLAWKVKKFIEIPVVKADYEVYDPKGNKLPSNLAEIPDFLKH 273
SS + + IV YN L WK + +PVV D V+D +G+++ S L D ++
Sbjct: 471 SSEVNLSDGKSLIVLAYNPLGWKRVDIVRLPVVGGDVSVHDSEGHEVESQLVPFTD--EY 528
Query: 274 IPTRKSFATHILYFLTELSPSSMTSFYV 357
+ RK H+ +L + SP+ + +++
Sbjct: 529 VALRK---YHVEAYLGQ-SPTQVPKYWL 552
>At5g66150.1 68418.m08333 glycosyl hydrolase family 38 protein
similar to lysosomal alpha-mannosidase SP:O09159 from
[Mus musculus]
Length = 1047
Score = 37.1 bits (82), Expect = 0.006
Identities = 25/75 (33%), Positives = 34/75 (45%), Gaps = 5/75 (6%)
Frame = +1
Query: 25 NKMKQGKSSKQPLFNYDRCHF-NESSCLTAETS----DNFIVTIYNSLAWKVKKFIEIPV 189
NK +G +K + +C N S C + E + + I+ YNSL W + I IPV
Sbjct: 458 NKAPKGGCTKPAIAFSQQCSLMNISYCPSTEETLPGQKSLILVAYNSLGWNRTEIIRIPV 517
Query: 190 VKADYEVYDPKGNKL 234
A V D GN L
Sbjct: 518 NDAGLSVEDSSGNTL 532
>At1g20720.1 68414.m02596 helicase-related similar to BRCA1-binding
helicase-like protein BACH1 (GI:13661819) Homo sapiens].;
Length = 1169
Score = 28.7 bits (61), Expect = 2.0
Identities = 18/53 (33%), Positives = 28/53 (52%), Gaps = 1/53 (1%)
Frame = +1
Query: 73 DRCHFNESSCLTAETSDNFIVTIYNSLAWKVKKFIEIPVVKADYEVYD-PKGN 228
+R F + LT E +F +++ NS A K +KF PV+ + E D P+ N
Sbjct: 978 ERYSFGGTRSLTLEAESSFNMSV-NSQALKRRKFTSSPVIDLEVENSDAPRPN 1029
>At1g28695.1 68414.m03534 expressed protein ; expression supported
by MPSS
Length = 329
Score = 27.5 bits (58), Expect = 4.7
Identities = 14/38 (36%), Positives = 17/38 (44%)
Frame = +1
Query: 70 YDRCHFNESSCLTAETSDNFIVTIYNSLAWKVKKFIEI 183
YDRC F C ET D V + + K FIE+
Sbjct: 110 YDRCRFKRLHCYKMETEDG--VDLEGEKVFMSKDFIEM 145
>At2g21630.1 68415.m02573 transport protein, putative similar to
Swiss-Prot:Q15436 protein transport protein Sec23A [Homo
sapiens]
Length = 761
Score = 27.1 bits (57), Expect = 6.2
Identities = 13/45 (28%), Positives = 20/45 (44%)
Frame = +1
Query: 142 YNSLAWKVKKFIEIPVVKADYEVYDPKGNKLPSNLAEIPDFLKHI 276
+N W K I + DY+ +P L S L+ P F+ H+
Sbjct: 544 FNPQRWVDKALINLCTWFGDYQKGNPSSFSLSSQLSIFPQFVFHL 588
>At3g01670.1 68416.m00098 expressed protein
Length = 822
Score = 26.6 bits (56), Expect = 8.3
Identities = 21/62 (33%), Positives = 29/62 (46%), Gaps = 1/62 (1%)
Frame = +1
Query: 187 VVKADYEVYDPKGNKLPSNLAEIPDFLKHIPTRKSFATHIL-YFLTELSPSSMTSFYVKK 363
V KA + KGN LAE ++ +IPT K F T + + L L P T F + +
Sbjct: 735 VSKASDMMVRAKGNLFSRGLAEFNEWEVNIPT-KGFLTALNDHLLMRLPPHHCTRFMLPE 793
Query: 364 HA 369
A
Sbjct: 794 TA 795
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.317 0.131 0.390
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 8,540,713
Number of Sequences: 28952
Number of extensions: 156046
Number of successful extensions: 384
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 383
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 384
length of database: 12,070,560
effective HSP length: 75
effective length of database: 9,899,160
effective search space used: 782033640
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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