Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= S06A01NCLL0002_B02
(341 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g62030.1 68418.m07786 diphthamide synthesis DPH2 family prote... 148 1e-36
At3g59630.1 68416.m06653 diphthamide synthesis DPH2 family prote... 57 3e-09
At5g20790.1 68418.m02470 expressed protein predicted protein, Ar... 29 1.1
At4g21250.1 68417.m03072 expressed protein contains Pfam profi... 27 2.5
At3g62820.1 68416.m07058 invertase/pectin methylesterase inhibit... 27 2.5
At2g16440.1 68415.m01883 DNA replication licensing factor, putat... 27 3.3
At1g65370.1 68414.m07416 meprin and TRAF homology domain-contain... 27 3.3
At5g48880.2 68418.m06047 acetyl-CoA C-acyltransferase 1 / 3-keto... 27 4.3
At1g78240.1 68414.m09118 dehydration-responsive protein-related ... 26 5.7
At1g31300.1 68414.m03830 expressed protein similar to hypothetic... 26 5.7
At5g66960.1 68418.m08442 prolyl oligopeptidase family protein si... 26 7.6
At3g52720.2 68416.m05809 carbonic anhydrase family protein low s... 26 7.6
At3g52720.1 68416.m05808 carbonic anhydrase family protein low s... 26 7.6
At3g46840.1 68416.m05084 subtilase family protein contains simil... 26 7.6
At1g62030.1 68414.m06996 DC1 domain-containing protein contains ... 26 7.6
>At5g62030.1 68418.m07786 diphthamide synthesis DPH2 family protein
contains InterPro accession IPR002728: Diphthamide
synthesis DPH2 protein
Length = 453
Score = 148 bits (358), Expect = 1e-36
Identities = 68/111 (61%), Positives = 83/111 (74%), Gaps = 1/111 (0%)
Frame = +1
Query: 10 LNRACEGLPQNYNFEIHKTIWRIRTLKAKRVALQMPEGLTMFATTLCDIVETFTDAD-TV 186
LN A LP Y+FE+HK +WRI+T AKR+ALQ+PEGL M+A TL DI +F A
Sbjct: 36 LNAAISILPSIYHFEVHKCVWRIKTSNAKRIALQLPEGLLMYALTLSDIFTSFAGASHCF 95
Query: 187 IMGDVTYGACCVDDFTAVALGVDLLVHYGHSCLIPIDQTNTIKVPYIFVDI 339
++GDVTYGACCVDDF+A ALG DLL+HYGHSCL+PID T I Y+FV+I
Sbjct: 96 VLGDVTYGACCVDDFSACALGADLLIHYGHSCLVPIDSTK-IPCLYVFVEI 145
>At3g59630.1 68416.m06653 diphthamide synthesis DPH2 family protein
contains InterPro accession IPR002728: Diphthamide
synthesis DPH2 protein
Length = 491
Score = 57.2 bits (132), Expect = 3e-09
Identities = 29/87 (33%), Positives = 50/87 (57%), Gaps = 6/87 (6%)
Frame = +1
Query: 49 FEIHKTIWRIRTLKAKRVALQMPEGLTMFATTLCDIVET----FTDADT--VIMGDVTYG 210
+EI++T I + R+ALQ P+ L +T + +++ TD + +M D TYG
Sbjct: 9 YEINRTAEFIISKSFTRIALQFPDELLKDSTKVVSALKSKTRLLTDREVRFFVMADTTYG 68
Query: 211 ACCVDDFTAVALGVDLLVHYGHSCLIP 291
+CC+D+ A+ + + +VHYG +CL P
Sbjct: 69 SCCIDEVGALHIDSECVVHYGQTCLSP 95
>At5g20790.1 68418.m02470 expressed protein predicted protein,
Arabidopsis thaliana
Length = 193
Score = 28.7 bits (61), Expect = 1.1
Identities = 14/57 (24%), Positives = 27/57 (47%)
Frame = +1
Query: 34 PQNYNFEIHKTIWRIRTLKAKRVALQMPEGLTMFATTLCDIVETFTDADTVIMGDVT 204
P++ + +T+ R++ K L +G+T T+C V+ D + GD+T
Sbjct: 76 PKSITGPVKETVARVKETVTKTEPLICDDGVTKGKLTMCYEVDVDVDGGRCVNGDLT 132
>At4g21250.1 68417.m03072 expressed protein contains Pfam profile:
PF01925 domain of unknown function DUF81
Length = 449
Score = 27.5 bits (58), Expect = 2.5
Identities = 24/83 (28%), Positives = 38/83 (45%), Gaps = 1/83 (1%)
Frame = +1
Query: 13 NRACEGLPQNYNFEIHKT-IWRIRTLKAKRVALQMPEGLTMFATTLCDIVETFTDADTVI 189
N+ E LPQ+++ +HK WR ++ L++ + M A LC + + A
Sbjct: 20 NQEEENLPQSHHNLLHKVQQWRTSLKESSAAELKLSSAIIM-AGVLCFLAALISSA---- 74
Query: 190 MGDVTYGACCVDDFTAVALGVDL 258
G + G + T VA GVDL
Sbjct: 75 -GGIGGGGLFIPIMTIVA-GVDL 95
>At3g62820.1 68416.m07058 invertase/pectin methylesterase inhibitor
family protein low similarity to pectinesterase from
Arabidopsis thaliana SP|Q43867, Lycopersicon esculentum
SP|Q43143; contains Pfam profile PF04043: Plant
invertase/pectin methylesterase inhibitor
Length = 194
Score = 27.5 bits (58), Expect = 2.5
Identities = 14/44 (31%), Positives = 24/44 (54%)
Frame = +1
Query: 22 CEGLPQNYNFEIHKTIWRIRTLKAKRVALQMPEGLTMFATTLCD 153
C L Q+ +HK++ +RTL+A QM + T +++L D
Sbjct: 103 CRELLQDSLDSLHKSLAVLRTLRASEFQQQMSDLATWLSSSLTD 146
>At2g16440.1 68415.m01883 DNA replication licensing factor, putative
similar to SP|P49717 DNA replication licensing factor
MCM4 (CDC21 homolog) {Mus musculus}, SP|P29458 Cdc21
protein {Schizosaccharomyces pombe}; contains Pfam
profile PF00493: MCM2/3/5 family
Length = 847
Score = 27.1 bits (57), Expect = 3.3
Identities = 12/49 (24%), Positives = 25/49 (51%)
Frame = +1
Query: 121 GLTMFATTLCDIVETFTDADTVIMGDVTYGACCVDDFTAVALGVDLLVH 267
GLT + + ET ++ +++ D G CC+D+F ++ ++H
Sbjct: 517 GLTAYVAKDPETGETVLESGALVLSD--RGICCIDEFDKMSDSARSMLH 563
>At1g65370.1 68414.m07416 meprin and TRAF homology domain-containing
protein / MATH domain-containing protein contains Pfam
profile PF00917: MATH domain
Length = 227
Score = 27.1 bits (57), Expect = 3.3
Identities = 14/43 (32%), Positives = 23/43 (53%)
Frame = +1
Query: 58 HKTIWRIRTLKAKRVALQMPEGLTMFATTLCDIVETFTDADTV 186
HKT+W + + A A +P+ L +L D+ E + D DT+
Sbjct: 175 HKTVWLQQWIMAATKARGIPQSL-----SLADLQEAYLDEDTL 212
>At5g48880.2 68418.m06047 acetyl-CoA C-acyltransferase 1 /
3-ketoacyl-CoA thiolase 1 (PKT1) identical to
3-keto-acyl-CoA-thiolase 1 [Arabidopsis thaliana]
GI:3169568
Length = 457
Score = 26.6 bits (56), Expect = 4.3
Identities = 14/48 (29%), Positives = 23/48 (47%)
Frame = -1
Query: 227 SSTQHAPYVTSPIITVSASVNVSTISHNVVANIVRPSGICKATLFAFR 84
SS +H P + SP+ VS ++ ++V + ICKA F+
Sbjct: 23 SSLKHEPSLLSPVNCVSEVSPMAAFGDDIVIVAAYRTAICKARRGGFK 70
>At1g78240.1 68414.m09118 dehydration-responsive protein-related
similar to early-responsive to dehydration stress ERD3
protein [Arabidopsis thaliana] GI:15320410; contains
Pfam profile PF03141: Putative methyltransferase
Length = 684
Score = 26.2 bits (55), Expect = 5.7
Identities = 15/51 (29%), Positives = 22/51 (43%), Gaps = 6/51 (11%)
Frame = -3
Query: 321 WNF--DSISLVNW----YKTGMAIMYKQVNTKCYSCKVIYTACSICDISHN 187
WNF D + W + + K +NTKCYS + S+C H+
Sbjct: 389 WNFVHDFAESICWTLLNQQDETVVWKKTINTKCYSSRKPGVGPSVCTKGHD 439
>At1g31300.1 68414.m03830 expressed protein similar to hypothetical
protein GB:AAF24587 GI:6692122 from [Arabidopsis
thaliana]
Length = 278
Score = 26.2 bits (55), Expect = 5.7
Identities = 11/28 (39%), Positives = 14/28 (50%)
Frame = -3
Query: 270 IMYKQVNTKCYSCKVIYTACSICDISHN 187
I Y V T + CKV+Y C SH+
Sbjct: 33 IPYTSVLTGIFLCKVVYDLCHFISNSHS 60
>At5g66960.1 68418.m08442 prolyl oligopeptidase family protein
similar to OpdB [Treponema denticola] GI:13786054;
contains Pfam profiles PF00326: prolyl oligopeptidase
family, PF02897: Prolyl oligopeptidase, N-terminal
beta-propeller domain
Length = 792
Score = 25.8 bits (54), Expect = 7.6
Identities = 9/29 (31%), Positives = 16/29 (55%)
Frame = -3
Query: 312 DSISLVNWYKTGMAIMYKQVNTKCYSCKV 226
D +S + W K G A++Y + K C++
Sbjct: 229 DRVSNIAWAKNGQALLYVVTDQKKRPCRI 257
>At3g52720.2 68416.m05809 carbonic anhydrase family protein low
similarity to storage protein (dioscorin) [Dioscorea
cayenensis] GI:433463; contains Pfam profile PF00194:
Eukaryotic-type carbonic anhydrase
Length = 230
Score = 25.8 bits (54), Expect = 7.6
Identities = 13/28 (46%), Positives = 15/28 (53%)
Frame = +3
Query: 66 YMEDTNSKSKESCFTNA*RSNNVCNYIM 149
Y NS +E FTNA N+VCN M
Sbjct: 70 YNHKLNSIHREYYFTNATLVNHVCNVAM 97
>At3g52720.1 68416.m05808 carbonic anhydrase family protein low
similarity to storage protein (dioscorin) [Dioscorea
cayenensis] GI:433463; contains Pfam profile PF00194:
Eukaryotic-type carbonic anhydrase
Length = 284
Score = 25.8 bits (54), Expect = 7.6
Identities = 13/28 (46%), Positives = 15/28 (53%)
Frame = +3
Query: 66 YMEDTNSKSKESCFTNA*RSNNVCNYIM 149
Y NS +E FTNA N+VCN M
Sbjct: 70 YNHKLNSIHREYYFTNATLVNHVCNVAM 97
>At3g46840.1 68416.m05084 subtilase family protein contains
similarity to prepro-cucumisin GI:807698 from [Cucumis
melo];
Length = 738
Score = 25.8 bits (54), Expect = 7.6
Identities = 21/75 (28%), Positives = 35/75 (46%), Gaps = 5/75 (6%)
Frame = -1
Query: 254 STPSATAVKSSTQHAPY-VTSPIIT--VSASVNVSTISHNVVANIVRPSGICKATLFA-- 90
S S++ K T+ P + P +T VSA+ I V N+ RP+ KA +
Sbjct: 620 SGDSSSCTKEQTKSLPRNLNYPSMTAQVSAAKPFKVIFRRTVTNVGRPNATYKAKVVGSK 679
Query: 89 FRVRILHIVLCISKL 45
+V+++ VL + L
Sbjct: 680 LKVKVVPAVLSLKSL 694
>At1g62030.1 68414.m06996 DC1 domain-containing protein contains
Pfam protein PF03107 DC1 domain
Length = 743
Score = 25.8 bits (54), Expect = 7.6
Identities = 13/54 (24%), Positives = 26/54 (48%), Gaps = 6/54 (11%)
Frame = -3
Query: 309 SISLVNWYKTGMAIMYKQVNTKCYSCKVIYTACSI-----CD-ISHNNRVSISK 166
SI W++ +A+ +Q C C + +T+C CD ++H +S+ +
Sbjct: 276 SIDHSKWHEHTLALFPRQTPFACSLCSLTHTSCPFYICPPCDFVAHQRCISLPR 329
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 7,490,423
Number of Sequences: 28952
Number of extensions: 144753
Number of successful extensions: 406
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 398
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 404
length of database: 12,070,560
effective HSP length: 72
effective length of database: 9,986,016
effective search space used: 409426656
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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