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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= S06A01NCLL0001_P19
         (546 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch...   297   7e-82
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar...   292   3e-80
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces...   159   3e-40
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|...   114   9e-27
SPAC3F10.06c |||initiator methionine tRNA 2'-O-ribosyl phosphate...    29   0.34 
SPBC1703.15c |vps33|SPBC2A9.01c|vacuolar sorting protein Vps33|S...    28   1.0  
SPBC13G1.08c |ash2||Ash2-trithorax family protein|Schizosaccharo...    27   1.4  
SPAC1486.04c |alm1||medial ring protein Alm1|Schizosaccharomyces...    26   3.2  
SPBC1826.01c |mot1||TATA-binding protein associated factor Mot1|...    26   4.2  
SPBC2D10.16 |||sequence orphan|Schizosaccharomyces pombe|chr 2||...    25   5.5  
SPAC1834.08 |mak1|phk3|histidine kinase Mak1|Schizosaccharomyces...    25   5.5  
SPAC869.01 |||amidase |Schizosaccharomyces pombe|chr 1|||Manual        25   5.5  
SPBC26H8.04c |||DEP domain|Schizosaccharomyces pombe|chr 2|||Manual    25   7.3  
SPAC688.08 |srb8|med12|mediator complex subunit Srb8 |Schizosacc...    25   7.3  
SPBC13G1.05 |||DUF747 family protein|Schizosaccharomyces pombe|c...    25   9.6  
SPAC1805.15c |pub2||ubiquitin-protein ligase Pub2|Schizosaccharo...    25   9.6  
SPAC23D3.13c |||guanyl-nucleotide exchange factor|Schizosaccharo...    25   9.6  
SPBC428.07 |meu6||meiotic chromosome segregation protein Meu6|Sc...    25   9.6  
SPAC521.04c |||calcium permease |Schizosaccharomyces pombe|chr 1...    25   9.6  

>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 455

 Score =  297 bits (729), Expect = 7e-82
 Identities = 131/179 (73%), Positives = 156/179 (87%)
 Frame = +3

Query: 9   AANNYARGHYTIGKEIVDLVLDRIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLS 188
           A+NNYARGHYT+GKE++D VL+RIR++AD C+GLQGFL+FH            LL+ERL+
Sbjct: 103 ASNNYARGHYTVGKEMIDSVLERIRRMADNCSGLQGFLVFHSFGGGTGSGLGALLLERLN 162

Query: 189 VDYGKKSKLEFAIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLD 368
           ++YGKKS L+F++YPAPQVST+VVEPYNS+LTTH TL++SDC FMVDNEA YDICRRNLD
Sbjct: 163 MEYGKKSNLQFSVYPAPQVSTSVVEPYNSVLTTHATLDNSDCTFMVDNEACYDICRRNLD 222

Query: 369 IERPTYTNLNRLIGQIVSSITDSLRFDGALNVDLTEFQTNLVPYPRIHFPLVTYAPVIS 545
           IERPTY NLNRLI Q+VSSIT SLRF G+LNVDL EFQTNLVPYPRIHFPLVTY+P++S
Sbjct: 223 IERPTYENLNRLIAQVVSSITASLRFAGSLNVDLNEFQTNLVPYPRIHFPLVTYSPIVS 281


>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
           2|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 449

 Score =  292 bits (716), Expect = 3e-80
 Identities = 129/179 (72%), Positives = 155/179 (86%)
 Frame = +3

Query: 9   AANNYARGHYTIGKEIVDLVLDRIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLS 188
           A+NNYARGHYT+GKE+VD V D+IR++AD C+GLQGFL+FH            LL+ERL+
Sbjct: 99  ASNNYARGHYTVGKELVDEVTDKIRRIADNCSGLQGFLVFHSFGGGTGSGFGALLLERLA 158

Query: 189 VDYGKKSKLEFAIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLD 368
           ++Y KKSKL+F++YPAPQVST+VVEPYNS+LTTH TL+ +DC FMVDNE+ YDICRRNLD
Sbjct: 159 MEYTKKSKLQFSVYPAPQVSTSVVEPYNSVLTTHATLDLADCTFMVDNESCYDICRRNLD 218

Query: 369 IERPTYTNLNRLIGQIVSSITDSLRFDGALNVDLTEFQTNLVPYPRIHFPLVTYAPVIS 545
           IERP+Y NLNRLI Q+VSSIT SLRF+G+LNVDL EFQTNLVPYPRIHFPLVTYAP++S
Sbjct: 219 IERPSYENLNRLIAQVVSSITASLRFEGSLNVDLAEFQTNLVPYPRIHFPLVTYAPIVS 277


>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 448

 Score =  159 bits (385), Expect = 3e-40
 Identities = 72/177 (40%), Positives = 108/177 (61%)
 Frame = +3

Query: 9   AANNYARGHYTIGKEIVDLVLDRIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLS 188
           A N +A+GHYT G E+ D VLD +R+ A+ C  LQGF + H            LL+ ++ 
Sbjct: 97  AGNIWAKGHYTEGAELADAVLDVVRREAEACDALQGFQLTHSLGGGTGSGMGTLLLSKIR 156

Query: 189 VDYGKKSKLEFAIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLD 368
            +Y  +    F++ PAP+ S  VVEPYN+ L+ H  +E+SD  F +DNEA+  I    L 
Sbjct: 157 EEYPDRMMATFSVAPAPKSSDTVVEPYNATLSMHQLVENSDETFCIDNEALSSIFANTLK 216

Query: 369 IERPTYTNLNRLIGQIVSSITDSLRFDGALNVDLTEFQTNLVPYPRIHFPLVTYAPV 539
           I+ P+Y +LN L+  +++ +T S RF G LN DL +   N+VP+PR+HF +V +AP+
Sbjct: 217 IKSPSYDDLNHLVSAVMAGVTTSFRFPGELNSDLRKLAVNMVPFPRLHFFMVGFAPL 273


>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 446

 Score =  114 bits (274), Expect = 9e-27
 Identities = 60/177 (33%), Positives = 95/177 (53%), Gaps = 1/177 (0%)
 Frame = +3

Query: 9   AANNYARGHYTIGKEIVDLVLDRIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLS 188
           A NN+A G Y+  + I + ++D I + AD    L+GF + H             L+ERL+
Sbjct: 100 AGNNWANG-YSHAERIFEDIMDMIDREADGSDSLEGFSLLHSIAGGTGSGLGSFLLERLN 158

Query: 189 VDYGKKSKLEFAIYPAPQ-VSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNL 365
             Y KK    ++++P  Q VS  VV+PYNS+L       ++D   ++DN A+  I    L
Sbjct: 159 DRYPKKIIQTYSVFPNSQSVSDVVVQPYNSLLALKRLTLNADSVVVLDNAALAHIAADRL 218

Query: 366 DIERPTYTNLNRLIGQIVSSITDSLRFDGALNVDLTEFQTNLVPYPRIHFPLVTYAP 536
             + PT+   N+L+  ++S+ T +LR+ G +N DL     +L+P PR HF L +Y P
Sbjct: 219 HTQNPTFHQQNQLVSTVMSASTTTLRYPGYMNNDLVSIIASLIPSPRCHFLLTSYTP 275


>SPAC3F10.06c |||initiator methionine tRNA 2'-O-ribosyl phosphate
           transferase |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 453

 Score = 29.5 bits (63), Expect = 0.34
 Identities = 11/38 (28%), Positives = 18/38 (47%)
 Frame = +1

Query: 310 IVLSWSTMKPSMTSAAVTWILNARPTPTSIVLSARSYH 423
           ++ S+ T KP +     TW +N R  P S+   +   H
Sbjct: 37  VIASYPTFKPVVNERCGTWYVNRRHAPISVYFKSTDGH 74


>SPBC1703.15c |vps33|SPBC2A9.01c|vacuolar sorting protein
           Vps33|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 592

 Score = 27.9 bits (59), Expect = 1.0
 Identities = 20/64 (31%), Positives = 30/64 (46%), Gaps = 1/64 (1%)
 Frame = +3

Query: 276 ILTTHTTLEHSDCAFMVDNEAI-YDICRRNLDIERPTYTNLNRLIGQIVSSITDSLRFDG 452
           I+TT+T  EH        NE I  DI ++ + + RPTY N   +   +     D LR + 
Sbjct: 39  IVTTNTLQEHGIPQVYWFNENIPNDIEKKTIYLCRPTYENAKLVATHVRQFQRDMLRIES 98

Query: 453 ALNV 464
            + V
Sbjct: 99  TVIV 102


>SPBC13G1.08c |ash2||Ash2-trithorax family
           protein|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 652

 Score = 27.5 bits (58), Expect = 1.4
 Identities = 15/32 (46%), Positives = 18/32 (56%), Gaps = 4/32 (12%)
 Frame = -1

Query: 84  CGCGRGPSLRFPFQWCS---DHEH-SCLRPSC 1
           C CG+  +LRFP   CS   +  H SCL P C
Sbjct: 43  CYCGKDRNLRFPDLQCSVCLNMFHLSCLSPPC 74


>SPAC1486.04c |alm1||medial ring protein Alm1|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 1727

 Score = 26.2 bits (55), Expect = 3.2
 Identities = 20/73 (27%), Positives = 34/73 (46%), Gaps = 1/73 (1%)
 Frame = -2

Query: 281 KDRVVGLYNSSGHLGCGIDSELQFRLLAVVNGESLHEERSETRTGATTERVEDEESL-EA 105
           KD++  L N + HLG  + S  Q           + E + +    +++ RV++E  + E 
Sbjct: 146 KDQIEALQNENSHLGEQVQSAHQAL-------SDIEERKKQHMFASSSSRVKEEILVQEK 198

Query: 104 CALVSEFADAVED 66
            ALVS+ A    D
Sbjct: 199 SALVSDLASLQSD 211


>SPBC1826.01c |mot1||TATA-binding protein associated factor
            Mot1|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 1953

 Score = 25.8 bits (54), Expect = 4.2
 Identities = 13/21 (61%), Positives = 13/21 (61%)
 Frame = +1

Query: 367  ILNARPTPTSIVLSARSYHRL 429
            I NAR   T  V S RSYHRL
Sbjct: 1499 IKNARAKLTKAVKSLRSYHRL 1519


>SPBC2D10.16 |||sequence orphan|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 135

 Score = 25.4 bits (53), Expect = 5.5
 Identities = 12/32 (37%), Positives = 19/32 (59%)
 Frame = -2

Query: 464 DIEGAVETQRVGNR*YDLADKTIEVGVGRAFN 369
           DIE   E    GN+ ++L +K I++  G+ FN
Sbjct: 58  DIEAFAEYALDGNKYFNLINKIIDMQDGKRFN 89


>SPAC1834.08 |mak1|phk3|histidine kinase Mak1|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 1639

 Score = 25.4 bits (53), Expect = 5.5
 Identities = 12/42 (28%), Positives = 22/42 (52%)
 Frame = +3

Query: 378 PTYTNLNRLIGQIVSSITDSLRFDGALNVDLTEFQTNLVPYP 503
           PT T ++ +   ++   +D     G   + +  F++NLVPYP
Sbjct: 493 PTETAVSIVHVPLMFPCSDQTSSRGRAPIAILSFKSNLVPYP 534


>SPAC869.01 |||amidase |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 583

 Score = 25.4 bits (53), Expect = 5.5
 Identities = 11/24 (45%), Positives = 17/24 (70%), Gaps = 1/24 (4%)
 Frame = -2

Query: 80  DAVEDQVYDFLSNGVVT-TSIVVC 12
           DA  DQ+ +++ NG++T T IV C
Sbjct: 68  DATIDQLQNYMENGILTSTDIVHC 91


>SPBC26H8.04c |||DEP domain|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 1496

 Score = 25.0 bits (52), Expect = 7.3
 Identities = 10/23 (43%), Positives = 14/23 (60%)
 Frame = +3

Query: 3   TRAANNYARGHYTIGKEIVDLVL 71
           +R+ NN   GH T+G + V L L
Sbjct: 887 SRSVNNQRNGHLTVGSDAVCLSL 909


>SPAC688.08 |srb8|med12|mediator complex subunit Srb8
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1233

 Score = 25.0 bits (52), Expect = 7.3
 Identities = 13/20 (65%), Positives = 14/20 (70%)
 Frame = +3

Query: 414 IVSSITDSLRFDGALNVDLT 473
           IVSSI D+  FD  L VDLT
Sbjct: 442 IVSSIFDNANFDRNLIVDLT 461


>SPBC13G1.05 |||DUF747 family protein|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 649

 Score = 24.6 bits (51), Expect = 9.6
 Identities = 10/21 (47%), Positives = 13/21 (61%)
 Frame = +3

Query: 6   RAANNYARGHYTIGKEIVDLV 68
           R ANN+ RGH  I K+  D +
Sbjct: 601 RKANNFFRGHIEIDKKTKDFL 621


>SPAC1805.15c |pub2||ubiquitin-protein ligase
           Pub2|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 671

 Score = 24.6 bits (51), Expect = 9.6
 Identities = 9/23 (39%), Positives = 13/23 (56%)
 Frame = +2

Query: 218 VRYLSRTPGVHCCCRALQLYPYN 286
           V ++S+ P  H C   L + PYN
Sbjct: 627 VGHISQLPKAHTCFNRLDIPPYN 649


>SPAC23D3.13c |||guanyl-nucleotide exchange factor|Schizosaccharomyces
            pombe|chr 1|||Manual
          Length = 1616

 Score = 24.6 bits (51), Expect = 9.6
 Identities = 11/50 (22%), Positives = 25/50 (50%), Gaps = 1/50 (2%)
 Frame = -2

Query: 179  LHEERSETRTGATTERVEDEESLEACALVSEFADAVEDQVYDFLS-NGVV 33
            LH +  +  +    ++ ED+    +C ++S  AD   + +   L+ NG++
Sbjct: 1078 LHSQPIQNVSDVNNKKDEDDHEQTSCLIISGIADVFSENMSLLLNVNGIL 1127


>SPBC428.07 |meu6||meiotic chromosome segregation protein
           Meu6|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 651

 Score = 24.6 bits (51), Expect = 9.6
 Identities = 10/25 (40%), Positives = 15/25 (60%)
 Frame = +3

Query: 453 ALNVDLTEFQTNLVPYPRIHFPLVT 527
           A +V++ E +TN +P    HFP  T
Sbjct: 368 AQSVEVPENETNQIPTTEEHFPATT 392


>SPAC521.04c |||calcium permease |Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 881

 Score = 24.6 bits (51), Expect = 9.6
 Identities = 11/31 (35%), Positives = 15/31 (48%)
 Frame = -2

Query: 200 AVVNGESLHEERSETRTGATTERVEDEESLE 108
           AVVNG S H    +  +   +    + ESLE
Sbjct: 662 AVVNGNSQHHHNRDDASSGLSSNGSENESLE 692


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,395,753
Number of Sequences: 5004
Number of extensions: 48350
Number of successful extensions: 172
Number of sequences better than 10.0: 19
Number of HSP's better than 10.0 without gapping: 168
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 171
length of database: 2,362,478
effective HSP length: 69
effective length of database: 2,017,202
effective search space used: 225926624
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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