Samia BLAST Result against arabidopsis

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= S06A01NCLL0001_N20
         (496 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At2g40290.2 68415.m04961 eukaryotic translation initiation facto...   157   4e-39
At2g40290.1 68415.m04960 eukaryotic translation initiation facto...   157   4e-39
At5g05470.1 68418.m00589 eukaryotic translation initiation facto...   152   1e-37
At3g23700.1 68416.m02980 S1 RNA-binding domain-containing protei...    31   0.43 
At1g74830.1 68414.m08670 expressed protein contains Pfam profile...    29   1.3  
At3g05530.1 68416.m00606 26S proteasome AAA-ATPase subunit (RPT5...    27   9.2  
At1g48920.1 68414.m05480 nucleolin, putative similar to nuM1 pro...    27   9.2  

>At2g40290.2 68415.m04961 eukaryotic translation initiation factor 2
           subunit 1, putative / eIF-2A, putative / eIF-2-alpha,
           putative similar to Swiss-Prot:P05198 eukaryotic
           translation initiation factor 2 subunit 1 (Eukaryotic
           translation initiation factor 2 alpha subunit)
           (eIF-2-alpha) (EIF- 2alpha) (EIF-2A) [Homo sapiens]
          Length = 241

 Score =  157 bits (381), Expect = 4e-39
 Identities = 78/133 (58%), Positives = 93/133 (69%)
 Frame = +2

Query: 92  LSCRFYQEKYPEVEDVVMVNVRSIAEMGAYVHLLEYNNIEGMXXXXXXXXXXXXXXNKLI 271
           L CR Y+ KYPEV+  VM+ V++IA+MGAYV LLEYNNIEGM              + LI
Sbjct: 8   LECRMYEAKYPEVDMAVMIQVKNIADMGAYVSLLEYNNIEGMILFSELSRRRIRSVSSLI 67

Query: 272 RVGKTEPVVVIRVDKEKGYIDLSKRRVSAEDIDKCTERYAKAKAVNSILRHVAELLHYES 451
           +VG+ EPV+V+RVDKEKGYIDLSKRRVS EDI  C ERY K+K V+SI+RHVAE L    
Sbjct: 68  KVGRIEPVMVLRVDKEKGYIDLSKRRVSEEDIQTCEERYNKSKLVHSIMRHVAETL---- 123

Query: 452 SEQLEELYKTTAW 490
           S  LE+LY    W
Sbjct: 124 SIDLEDLYVNIGW 136


>At2g40290.1 68415.m04960 eukaryotic translation initiation factor 2
           subunit 1, putative / eIF-2A, putative / eIF-2-alpha,
           putative similar to Swiss-Prot:P05198 eukaryotic
           translation initiation factor 2 subunit 1 (Eukaryotic
           translation initiation factor 2 alpha subunit)
           (eIF-2-alpha) (EIF- 2alpha) (EIF-2A) [Homo sapiens]
          Length = 344

 Score =  157 bits (381), Expect = 4e-39
 Identities = 78/133 (58%), Positives = 93/133 (69%)
 Frame = +2

Query: 92  LSCRFYQEKYPEVEDVVMVNVRSIAEMGAYVHLLEYNNIEGMXXXXXXXXXXXXXXNKLI 271
           L CR Y+ KYPEV+  VM+ V++IA+MGAYV LLEYNNIEGM              + LI
Sbjct: 8   LECRMYEAKYPEVDMAVMIQVKNIADMGAYVSLLEYNNIEGMILFSELSRRRIRSVSSLI 67

Query: 272 RVGKTEPVVVIRVDKEKGYIDLSKRRVSAEDIDKCTERYAKAKAVNSILRHVAELLHYES 451
           +VG+ EPV+V+RVDKEKGYIDLSKRRVS EDI  C ERY K+K V+SI+RHVAE L    
Sbjct: 68  KVGRIEPVMVLRVDKEKGYIDLSKRRVSEEDIQTCEERYNKSKLVHSIMRHVAETL---- 123

Query: 452 SEQLEELYKTTAW 490
           S  LE+LY    W
Sbjct: 124 SIDLEDLYVNIGW 136


>At5g05470.1 68418.m00589 eukaryotic translation initiation factor 2
           subunit 1, putative / eIF-2A, putative / eIF-2-alpha,
           putative similar to SWISS-PROT:P20459 eukaryotic
           translation initiation factor 2 alpha subunit (eIF-2-
           alpha) [Saccharomyces cerevisiae]; identical to cDNA
           cohesin GI:6682280
          Length = 344

 Score =  152 bits (368), Expect = 1e-37
 Identities = 73/133 (54%), Positives = 94/133 (70%)
 Frame = +2

Query: 92  LSCRFYQEKYPEVEDVVMVNVRSIAEMGAYVHLLEYNNIEGMXXXXXXXXXXXXXXNKLI 271
           L CR Y+ +YP+V+  VM+ V++IA+MGAYV LLEYNNIEGM              + LI
Sbjct: 8   LECRMYESRYPDVDMAVMIQVKTIADMGAYVSLLEYNNIEGMILFSELSRRRIRSISSLI 67

Query: 272 RVGKTEPVVVIRVDKEKGYIDLSKRRVSAEDIDKCTERYAKAKAVNSILRHVAELLHYES 451
           +VG+TEPV+V+RVD+E+GYIDLSKRRVS ED + C ERY K+K V+SI+RHVAE +  + 
Sbjct: 68  KVGRTEPVMVLRVDRERGYIDLSKRRVSDEDKEACEERYNKSKLVHSIMRHVAETVGVD- 126

Query: 452 SEQLEELYKTTAW 490
              LEELY    W
Sbjct: 127 ---LEELYVNIGW 136


>At3g23700.1 68416.m02980 S1 RNA-binding domain-containing protein
           contains Pfam domain, PF00575: S1 RNA binding domain
          Length = 392

 Score = 31.1 bits (67), Expect = 0.43
 Identities = 21/83 (25%), Positives = 37/83 (44%), Gaps = 3/83 (3%)
 Frame = +2

Query: 128 VEDVVMVNVRSIAEMGAYVHLL---EYNNIEGMXXXXXXXXXXXXXXNKLIRVGKTEPVV 298
           V DV    V S+ + GA++HL       ++ G+                ++R G    V+
Sbjct: 195 VGDVFNGRVGSVEDYGAFIHLRFDDGLYHLTGLVHVSEVSWDYVQDVRDVLRDGDEVRVI 254

Query: 299 VIRVDKEKGYIDLSKRRVSAEDI 367
           V  +DKEK  I LS +++  + +
Sbjct: 255 VTNIDKEKSRITLSIKQLEDDPL 277


>At1g74830.1 68414.m08670 expressed protein contains Pfam profile
           PF04576: Protein of unknown function, DUF593
          Length = 542

 Score = 29.5 bits (63), Expect = 1.3
 Identities = 15/55 (27%), Positives = 31/55 (56%)
 Frame = +2

Query: 299 VIRVDKEKGYIDLSKRRVSAEDIDKCTERYAKAKAVNSILRHVAELLHYESSEQL 463
           V+  D+EKG    SK  +  +++ + TER +  ++   +L+H+A++L     E +
Sbjct: 467 VVSADEEKG--SESKEGI-VKELSEITERLSTLQSNGDLLKHIADVLDVSEGEAI 518


>At3g05530.1 68416.m00606 26S proteasome AAA-ATPase subunit (RPT5a)
           identical to GB:AAF22525 GI:6652886 from [Arabidopsis
           thaliana]
          Length = 424

 Score = 26.6 bits (56), Expect = 9.2
 Identities = 14/45 (31%), Positives = 23/45 (51%)
 Frame = +2

Query: 68  KEYKHKMPLSCRFYQEKYPEVEDVVMVNVRSIAEMGAYVHLLEYN 202
           KE   +  L C  Y+EK  E ++ + +N +    +G  V +LE N
Sbjct: 40  KEDAQRTNLECDSYKEKIKENQEKIKLNKQLPYLVGNIVEILEMN 84


>At1g48920.1 68414.m05480 nucleolin, putative similar to nuM1
           protein GI:1279562 from [Medicago sativa]
          Length = 557

 Score = 26.6 bits (56), Expect = 9.2
 Identities = 15/46 (32%), Positives = 24/46 (52%)
 Frame = +2

Query: 269 IRVGKTEPVVVIRVDKEKGYIDLSKRRVSAEDIDKCTERYAKAKAV 406
           + + K +  V+  V KEK    + K+  S++D D  +E   KAK V
Sbjct: 35  VSLKKQKKDVIAAVQKEKAVKKVPKKVESSDDSDSESEEEEKAKKV 80


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 9,610,233
Number of Sequences: 28952
Number of extensions: 167236
Number of successful extensions: 423
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 421
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 423
length of database: 12,070,560
effective HSP length: 76
effective length of database: 9,870,208
effective search space used: 868578304
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)