BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= S06A01NCLL0001_N16
(553 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC1687.06c |rpl44|rpl28|60S ribosomal protein L28/L44|Schizosa... 59 5e-10
SPAC31A2.06 |||conserved fungal protein|Schizosaccharomyces pomb... 26 4.2
SPAC6F12.05c |tnr3||thiamine diphosphokinase Tnr3 |Schizosacchar... 25 7.4
SPAC222.10c |byr4||two-component GAP Byr4|Schizosaccharomyces po... 25 7.4
SPCC1902.01 |gaf1|SPCC417.01c|transcription factor Gaf1 |Schizos... 25 9.8
SPBC8D2.05c |sfi1||spindle pole body protein Sfi1|Schizosaccharo... 25 9.8
>SPAC1687.06c |rpl44|rpl28|60S ribosomal protein
L28/L44|Schizosaccharomyces pombe|chr 1|||Manual
Length = 134
Score = 58.8 bits (136), Expect = 5e-10
Identities = 38/110 (34%), Positives = 56/110 (50%), Gaps = 1/110 (0%)
Frame = +3
Query: 54 MSSHLNWMIIRNNNAFLVKKANIKK-PFSKEPNNVTNLNSYRYNGLIHKKAVGVVENPDR 230
+S+ L W +IR+NN FLVK+ F++EP NV+ N+ R++GL + KAVGV N R
Sbjct: 3 VSNDLIWQVIRDNNRFLVKRPEFGGIQFNREPVNVSGKNAQRFSGLCNDKAVGVQANSPR 62
Query: 231 KGFTIVYKKAKATNKPVXEHYPSSIQSWSEEVSFQSQEIAKGKSLPYRPD 380
I K KP I + S +++S G++ YR D
Sbjct: 63 GVVLITKTNPKNAQKPAKLFRKDVIANASSRKTYKSIAGRIGRT-GYRDD 111
Score = 33.1 bits (72), Expect = 0.028
Identities = 15/22 (68%), Positives = 17/22 (77%)
Frame = +1
Query: 364 YRTDLTKATLRRASAILRSQRP 429
YR DL K ++ RASAIL SQRP
Sbjct: 108 YRDDLVKVSVARASAILSSQRP 129
>SPAC31A2.06 |||conserved fungal protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 542
Score = 25.8 bits (54), Expect = 4.2
Identities = 12/27 (44%), Positives = 14/27 (51%)
Frame = +1
Query: 301 PFKAGARRFLFKAKRLLKANHYRTDLT 381
P GA FL LLK +Y TDL+
Sbjct: 81 PIPKGAPNFLADVLNLLKKKYYATDLS 107
>SPAC6F12.05c |tnr3||thiamine diphosphokinase Tnr3
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 569
Score = 25.0 bits (52), Expect = 7.4
Identities = 14/36 (38%), Positives = 21/36 (58%)
Frame = -1
Query: 376 GLYGNDLPLAISWL*KETSSLQL*MDDG*CSFTGLL 269
GL+ LP+ S K+TS L+ ++D C F GL+
Sbjct: 507 GLHCGLLPVGQSVYVKKTSGLEWNIEDRICQFGGLV 542
>SPAC222.10c |byr4||two-component GAP Byr4|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 665
Score = 25.0 bits (52), Expect = 7.4
Identities = 19/83 (22%), Positives = 30/83 (36%)
Frame = +3
Query: 81 IRNNNAFLVKKANIKKPFSKEPNNVTNLNSYRYNGLIHKKAVGVVENPDRKGFTIVYKKA 260
IR+ N K I S +PN + Y I ENP + Y
Sbjct: 246 IRSPNTINQKHPYISSTISYQPNVHQDAKYYPLCKDIFPSLAN--ENPHSDNPNLKYSSK 303
Query: 261 KATNKPVXEHYPSSIQSWSEEVS 329
+ + HYP ++++ S+ S
Sbjct: 304 TLSKRDTSSHYPETLKASSKHSS 326
>SPCC1902.01 |gaf1|SPCC417.01c|transcription factor Gaf1
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 855
Score = 24.6 bits (51), Expect = 9.8
Identities = 11/27 (40%), Positives = 15/27 (55%)
Frame = -2
Query: 324 PPRSSFEWTTDNVXLQVYWLPWPSYKL 244
PP SS E T DN ++ P P+Y +
Sbjct: 101 PPTSSAETTADNSDTEMKLNPIPAYSV 127
>SPBC8D2.05c |sfi1||spindle pole body protein
Sfi1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 840
Score = 24.6 bits (51), Expect = 9.8
Identities = 9/18 (50%), Positives = 12/18 (66%)
Frame = +3
Query: 69 NWMIIRNNNAFLVKKANI 122
NWM R N L++KAN+
Sbjct: 289 NWMSKRRRNESLIQKANV 306
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,974,206
Number of Sequences: 5004
Number of extensions: 34677
Number of successful extensions: 94
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 91
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 93
length of database: 2,362,478
effective HSP length: 69
effective length of database: 2,017,202
effective search space used: 229961028
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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