BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= S06A01NCLL0001_N08 (342 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At3g24450.1 68416.m03068 copper-binding family protein similar t... 27 2.5 At1g45403.1 68414.m05196 membrane protein 26 5.7 At5g62760.2 68418.m07879 nuclear protein ZAP-related similar to ... 26 7.6 At5g62760.1 68418.m07878 nuclear protein ZAP-related similar to ... 26 7.6 At5g51770.1 68418.m06419 protein kinase family protein contains ... 26 7.6 At3g62910.1 68416.m07067 peptide chain release factor, putative ... 25 10.0 At3g61780.1 68416.m06931 expressed protein ; expression supporte... 25 10.0 At3g51180.1 68416.m05603 zinc finger (CCCH-type) family protein ... 25 10.0 At1g55220.1 68414.m06307 hypothetical protein 25 10.0 At1g01840.1 68414.m00103 expressed protein 25 10.0 >At3g24450.1 68416.m03068 copper-binding family protein similar to copper homeostasis factor gi:3168840 from Arabidopsis thaliana; contains Pfam profile PF00403: Heavy-metal-associated domain Length = 140 Score = 27.5 bits (58), Expect = 2.5 Identities = 13/29 (44%), Positives = 18/29 (62%) Frame = -2 Query: 107 LAMHIYGCNKKIREGNCNLLSLHKWQKVE 21 ++MH YGC KK+ E + + L W KVE Sbjct: 80 VSMHCYGCAKKV-EKHISKLDGVTWYKVE 107 >At1g45403.1 68414.m05196 membrane protein Length = 169 Score = 26.2 bits (55), Expect = 5.7 Identities = 13/40 (32%), Positives = 21/40 (52%) Frame = -1 Query: 282 LLAVEPKLRTTHHKVQRAADSASL*VSM*RNISHGLYRFI 163 +L + L THHK+ AD +SL V + + G+ F+ Sbjct: 41 ILNIAANLYMTHHKIPLTADESSLTVCLLMYLVCGMMSFM 80 >At5g62760.2 68418.m07879 nuclear protein ZAP-related similar to nuclear protein ZAP, Mus musculus, EMBL:AB033168 this cDNA provides a truncated ORF likely due to a skipped exon. An alternative ORF is provided. Length = 383 Score = 25.8 bits (54), Expect = 7.6 Identities = 10/25 (40%), Positives = 15/25 (60%) Frame = +2 Query: 266 GSTARSPLPTSPPATPRQINPNALY 340 GS + PLP SPP +P++L+ Sbjct: 198 GSNGQPPLPVSPPPPLPSSHPSSLF 222 >At5g62760.1 68418.m07878 nuclear protein ZAP-related similar to nuclear protein ZAP, Mus musculus, EMBL:AB033168 this cDNA provides a truncated ORF likely due to a skipped exon. An alternative ORF is provided. Length = 661 Score = 25.8 bits (54), Expect = 7.6 Identities = 10/25 (40%), Positives = 15/25 (60%) Frame = +2 Query: 266 GSTARSPLPTSPPATPRQINPNALY 340 GS + PLP SPP +P++L+ Sbjct: 198 GSNGQPPLPVSPPPPLPSSHPSSLF 222 >At5g51770.1 68418.m06419 protein kinase family protein contains protein kinase domain, Pfam:PF00069 Length = 654 Score = 25.8 bits (54), Expect = 7.6 Identities = 13/34 (38%), Positives = 16/34 (47%) Frame = +2 Query: 221 ESAARCTL*CVVRNLGSTARSPLPTSPPATPRQI 322 ES T C N G T +SP+P S P Q+ Sbjct: 269 ESVVTNTTGCDESNFGFTDQSPVPLSSPEMVEQV 302 >At3g62910.1 68416.m07067 peptide chain release factor, putative similar to peptide chain release factor 1 [Escherichia coli] GI:147567; contains Pfam profiles PF00472: Peptidyl-tRNA hydrolase domain, PF03462: PCRF domain Length = 422 Score = 25.4 bits (53), Expect = 10.0 Identities = 14/43 (32%), Positives = 19/43 (44%), Gaps = 1/43 (2%) Frame = -2 Query: 299 ARSGGDSLQ-WNLNYVRHITKYNEPQIRRVSRFRCEETSHMVY 174 A +GGD W + VR +Y+E + S C E H Y Sbjct: 173 AGTGGDEAAIWTGDLVRMYQRYSERSSWKFSMVSCSEAEHGGY 215 >At3g61780.1 68416.m06931 expressed protein ; expression supported by MPSS Length = 1121 Score = 25.4 bits (53), Expect = 10.0 Identities = 10/24 (41%), Positives = 14/24 (58%) Frame = -2 Query: 104 AMHIYGCNKKIREGNCNLLSLHKW 33 AM + G KK+ + N L LH+W Sbjct: 718 AMFLQGLEKKVEKENEKLSHLHQW 741 >At3g51180.1 68416.m05603 zinc finger (CCCH-type) family protein contains Pfam domain, PF00642: Zinc finger C-x8-C-x5-C-x3-H type (and similar) Length = 521 Score = 25.4 bits (53), Expect = 10.0 Identities = 12/37 (32%), Positives = 20/37 (54%) Frame = -2 Query: 332 HSGLSAAVLREARSGGDSLQWNLNYVRHITKYNEPQI 222 H G++ + AR SL NLN+ R ++EP++ Sbjct: 336 HVGIAKEQPQPARFPSSSLPMNLNFHRPPNVFSEPKV 372 >At1g55220.1 68414.m06307 hypothetical protein Length = 164 Score = 25.4 bits (53), Expect = 10.0 Identities = 12/40 (30%), Positives = 23/40 (57%), Gaps = 2/40 (5%) Frame = -2 Query: 125 FANVSLLAMH--IYGCNKKIREGNCNLLSLHKWQKVEFHF 12 F+N + M GC+K++ G C++ +H+ Q+ +HF Sbjct: 91 FSNSGTMVMKKKYLGCDKRV--GCCSMNDIHQLQRNLYHF 128 >At1g01840.1 68414.m00103 expressed protein Length = 152 Score = 25.4 bits (53), Expect = 10.0 Identities = 17/53 (32%), Positives = 21/53 (39%) Frame = -3 Query: 340 IESIRVYLPRCCGRRGREGTPCSGT*ITYDTSQSTTSRRFGESLGFDVKKHLT 182 IE + + C R R P S + +TS T R G GFD H T Sbjct: 89 IEKVEEKMRECFIRNKRLKRPLSPSSAVVETS--ATEERSGRDYGFDSDPHAT 139 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 7,826,605 Number of Sequences: 28952 Number of extensions: 148282 Number of successful extensions: 406 Number of sequences better than 10.0: 10 Number of HSP's better than 10.0 without gapping: 398 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 406 length of database: 12,070,560 effective HSP length: 72 effective length of database: 9,986,016 effective search space used: 409426656 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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