BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= S06A01NCLL0001_M22 (423 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At5g13190.1 68418.m01510 expressed protein 27 5.2 At2g39930.1 68415.m04907 isoamylase, putative / starch debranchi... 27 6.9 >At5g13190.1 68418.m01510 expressed protein Length = 134 Score = 27.1 bits (57), Expect = 5.2 Identities = 9/24 (37%), Positives = 14/24 (58%) Frame = -3 Query: 109 CFLCGR*VSCYYHRSAHCPQANQR 38 CFLC + C +++ HCPQ + Sbjct: 85 CFLCPS-MDCLWNKQHHCPQCGNK 107 >At2g39930.1 68415.m04907 isoamylase, putative / starch debranching enzyme, putative similar to isoamylase from [Solanum tuberosum] GI:27728145, [Triticum aestivum] GI:17932898, [Hordeum vulgare] GI:21314275, [Oryza sativa] GI:3252794; contains Pfam profiles PF00128: Alpha amylase catalytic domain, PF02922: Isoamylase N-terminal domain Length = 783 Score = 26.6 bits (56), Expect = 6.9 Identities = 14/36 (38%), Positives = 21/36 (58%) Frame = +3 Query: 99 HKKQSTRFSNYSWTKLESTNRKALVVNSSILAQRSR 206 H K +T FSN+++ K+ + N K L SI A+ R Sbjct: 13 HTKLNTLFSNHTFPKISAPNFKPLFRPISISAKDRR 48 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 7,280,138 Number of Sequences: 28952 Number of extensions: 111196 Number of successful extensions: 334 Number of sequences better than 10.0: 2 Number of HSP's better than 10.0 without gapping: 330 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 334 length of database: 12,070,560 effective HSP length: 74 effective length of database: 9,928,112 effective search space used: 655255392 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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