Samia BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= S06A01NCLL0001_L19
         (551 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces...   136   2e-33
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|...    81   8e-17
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar...    70   2e-13
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch...    65   7e-12
SPCC1223.03c |gut2||glycerol-3-phosphate dehydrogenase Gut2|Schi...    28   0.80 
SPAC6C3.08 |||gankyrin|Schizosaccharomyces pombe|chr 1|||Manual        27   1.4  
SPBC19G7.16 |iws1||transcription elongation factor complex subun...    27   1.8  
SPAC57A10.02 |cdr2||GIN4 family protein kinase Cdr2|Schizosaccha...    26   3.2  
SPBC215.01 ||SPBC3B9.20|GTPase activating protein|Schizosaccharo...    26   4.2  
SPBC14F5.08 |med7||mediator complex subunit Med7|Schizosaccharom...    25   5.6  
SPCC962.06c |bpb1|sf1|zinc finger protein Bpb1|Schizosaccharomyc...    25   7.4  
SPBC887.11 |pus2||tRNA pseudouridylate synthase Pus2 |Schizosacc...    25   7.4  
SPAC20H4.09 |||ATP-dependent RNA helicase, spliceosomal |Schizos...    25   9.8  

>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 448

 Score =  136 bits (329), Expect = 2e-33
 Identities = 57/87 (65%), Positives = 70/87 (80%)
 Frame = +3

Query: 105 QCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERINVYYNEASGGKYVPRAILVDLEP 284
           QCGNQ+GA FW  I+DEHG+D  G YHG S+ Q ER+NVY+NEA+GGKYVPRA+LVDLEP
Sbjct: 11  QCGNQVGAAFWSTIADEHGLDSAGIYHGTSEAQHERLNVYFNEAAGGKYVPRAVLVDLEP 70

Query: 285 GTMDSVRSEPFGQIFLPDNVCFPDSPA 365
           GTMD+V+S  FG +F PDN+ +  S A
Sbjct: 71  GTMDAVKSGKFGNLFRPDNIIYGQSGA 97



 Score = 82.2 bits (194), Expect = 5e-17
 Identities = 39/66 (59%), Positives = 43/66 (65%)
 Frame = +2

Query: 353 GQSGAGNNWAKGHYTEGAELVDSVLDVVRKESESCDCLQ*FQXXXXXXXXXXXXXXXXXX 532
           GQSGAGN WAKGHYTEGAEL D+VLDVVR+E+E+CD LQ FQ                  
Sbjct: 93  GQSGAGNIWAKGHYTEGAELADAVLDVVRREAEACDALQGFQLTHSLGGGTGSGMGTLLL 152

Query: 533 XKIREE 550
            KIREE
Sbjct: 153 SKIREE 158


>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 446

 Score = 81.4 bits (192), Expect = 8e-17
 Identities = 31/80 (38%), Positives = 56/80 (70%)
 Frame = +3

Query: 105 QCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERINVYYNEASGGKYVPRAILVDLEP 284
           QCGNQIG++FW+ +  EHGI P G     +   ++R +V++ ++   +Y+PRAIL+DLEP
Sbjct: 12  QCGNQIGSQFWQQLCLEHGIGPDGTLESFATEGVDRKDVFFYQSDDTRYIPRAILIDLEP 71

Query: 285 GTMDSVRSEPFGQIFLPDNV 344
             ++++ S+ +G ++ P+N+
Sbjct: 72  RVVNNILSDTYGSLYNPENI 91



 Score = 37.5 bits (83), Expect = 0.001
 Identities = 14/38 (36%), Positives = 25/38 (65%)
 Frame = +2

Query: 362 GAGNNWAKGHYTEGAELVDSVLDVVRKESESCDCLQ*F 475
           GAGNNWA G Y+    + + ++D++ +E++  D L+ F
Sbjct: 99  GAGNNWANG-YSHAERIFEDIMDMIDREADGSDSLEGF 135


>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
           2|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 449

 Score = 70.1 bits (164), Expect = 2e-13
 Identities = 33/82 (40%), Positives = 48/82 (58%), Gaps = 2/82 (2%)
 Frame = +3

Query: 105 QCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLER--INVYYNEASGGKYVPRAILVDL 278
           Q G QIG   WE+   EHGI P G  + ++  Q      + +++E   GKYVPR+I VDL
Sbjct: 11  QAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGKYVPRSIYVDL 70

Query: 279 EPGTMDSVRSEPFGQIFLPDNV 344
           EP  +D VR+ P+  +F P+ +
Sbjct: 71  EPNVIDQVRTGPYRDLFHPEQL 92



 Score = 52.8 bits (121), Expect = 3e-08
 Identities = 22/46 (47%), Positives = 33/46 (71%)
 Frame = +2

Query: 338 QRLFSGQSGAGNNWAKGHYTEGAELVDSVLDVVRKESESCDCLQ*F 475
           ++L +G+  A NN+A+GHYT G ELVD V D +R+ +++C  LQ F
Sbjct: 90  EQLITGKEDASNNYARGHYTVGKELVDEVTDKIRRIADNCSGLQGF 135


>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 455

 Score = 64.9 bits (151), Expect = 7e-12
 Identities = 32/86 (37%), Positives = 47/86 (54%), Gaps = 6/86 (6%)
 Frame = +3

Query: 105 QCGNQIGAKFWEIISDEHGIDPTG------AYHGDSDLQLERINVYYNEASGGKYVPRAI 266
           Q G QIG   WE+   EHGI P G        H ++    +    +++E   GK+VPR+I
Sbjct: 11  QAGVQIGNACWELYCLEHGIGPDGFPTENSEVHKNNSYLNDGFGTFFSETGQGKFVPRSI 70

Query: 267 LVDLEPGTMDSVRSEPFGQIFLPDNV 344
            VDLEP  +D VR+ P+  +F P+ +
Sbjct: 71  YVDLEPNVIDQVRTGPYKDLFHPEQM 96



 Score = 52.4 bits (120), Expect = 4e-08
 Identities = 20/46 (43%), Positives = 35/46 (76%)
 Frame = +2

Query: 338 QRLFSGQSGAGNNWAKGHYTEGAELVDSVLDVVRKESESCDCLQ*F 475
           +++ +G+  A NN+A+GHYT G E++DSVL+ +R+ +++C  LQ F
Sbjct: 94  EQMVTGKEDASNNYARGHYTVGKEMIDSVLERIRRMADNCSGLQGF 139


>SPCC1223.03c |gut2||glycerol-3-phosphate dehydrogenase
           Gut2|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 649

 Score = 28.3 bits (60), Expect = 0.80
 Identities = 20/60 (33%), Positives = 34/60 (56%), Gaps = 1/60 (1%)
 Frame = -2

Query: 430 VKN*VD*LSALCVVSLGPVVTGAGLSGKQTLSGRNICPKGSERTE-SMVPGSRSTRMARG 254
           +KN V+ +S   V+ +G   TG G++   +  G N+C    E+T+ +    S+ST+MA G
Sbjct: 59  LKN-VEDISKFDVLIIGGGATGTGVAVDASTRGLNVCL--LEKTDFASETSSKSTKMAHG 115


>SPAC6C3.08 |||gankyrin|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 234

 Score = 27.5 bits (58), Expect = 1.4
 Identities = 13/30 (43%), Positives = 19/30 (63%)
 Frame = +2

Query: 380 AKGHYTEGAELVDSVLDVVRKESESCDCLQ 469
           A+GH   G ELV +  D +RK+SE+   L+
Sbjct: 183 AEGHPDVGVELVRAGADTLRKDSENHTALE 212


>SPBC19G7.16 |iws1||transcription elongation factor complex subunit
           Iws1 |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 428

 Score = 27.1 bits (57), Expect = 1.8
 Identities = 10/34 (29%), Positives = 22/34 (64%)
 Frame = +3

Query: 114 NQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERI 215
           N++G    E+++++  +DPT A   + DLQ++ +
Sbjct: 133 NELGENEEEVLTEQKQLDPTLAAKKELDLQMDAV 166


>SPAC57A10.02 |cdr2||GIN4 family protein kinase
           Cdr2|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 775

 Score = 26.2 bits (55), Expect = 3.2
 Identities = 10/28 (35%), Positives = 15/28 (53%)
 Frame = +3

Query: 288 TMDSVRSEPFGQIFLPDNVCFPDSPAPV 371
           TM+ +R  PF   F+  N+  P   AP+
Sbjct: 252 TMEQIREHPFLSCFVHPNISIPIISAPI 279


>SPBC215.01 ||SPBC3B9.20|GTPase activating
           protein|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 834

 Score = 25.8 bits (54), Expect = 4.2
 Identities = 14/55 (25%), Positives = 26/55 (47%)
 Frame = +3

Query: 210 RINVYYNEASGGKYVPRAILVDLEPGTMDSVRSEPFGQIFLPDNVCFPDSPAPVT 374
           RI++  +E +   +  + +  +L     D  RS  F + F  + +C P+ P P T
Sbjct: 113 RISLQVSETTAAHFC-QLLREELVSHKADMQRSSEFSKQFFSERLCKPNVPEPET 166


>SPBC14F5.08 |med7||mediator complex subunit
           Med7|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 376

 Score = 25.4 bits (53), Expect = 5.6
 Identities = 12/28 (42%), Positives = 17/28 (60%)
 Frame = +3

Query: 24  PSYVSNLFTILLN*NKK*NEERAHSSRQ 107
           PS V N+  +LLN +   N+ R H SR+
Sbjct: 254 PSKVENIRVLLLNLHHLINDYRPHQSRE 281


>SPCC962.06c |bpb1|sf1|zinc finger protein Bpb1|Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 587

 Score = 25.0 bits (52), Expect = 7.4
 Identities = 20/76 (26%), Positives = 29/76 (38%), Gaps = 1/76 (1%)
 Frame = -2

Query: 346 QTLSGRNICPKGSERTESMVPGSRSTRMARGTYLPPEASL**TLMRSSCKSESPWYAPVG 167
           Q L G +    G+   +  +  S S   +     PP  +   T + SS  S +PW  P  
Sbjct: 372 QELGGGSAISNGNGEPQKSIEFSESGAASPQAGHPPPWAAASTSVSSSTSSPAPWAKPAS 431

Query: 166 S-MPCSSEMISQNLAP 122
           S  P +     Q  AP
Sbjct: 432 SAAPSNPAPWQQPAAP 447


>SPBC887.11 |pus2||tRNA pseudouridylate synthase Pus2
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 451

 Score = 25.0 bits (52), Expect = 7.4
 Identities = 13/27 (48%), Positives = 16/27 (59%)
 Frame = -3

Query: 381 AQLLPAPDCPENKRCQAGISVRKVPNE 301
           A L P P CP  K+ Q  +S RK+ NE
Sbjct: 165 ALLPPKPSCPLFKKMQKNLS-RKLDNE 190


>SPAC20H4.09 |||ATP-dependent RNA helicase, spliceosomal
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 647

 Score = 24.6 bits (51), Expect = 9.8
 Identities = 12/31 (38%), Positives = 17/31 (54%)
 Frame = -3

Query: 387 PLAQLLPAPDCPENKRCQAGISVRKVPNEQS 295
           P AQ LP+ DC +  +C     VR V + Q+
Sbjct: 556 PTAQRLPSSDCSKILKCLLDGFVRNVAHLQN 586


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,986,804
Number of Sequences: 5004
Number of extensions: 35903
Number of successful extensions: 98
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 91
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 96
length of database: 2,362,478
effective HSP length: 69
effective length of database: 2,017,202
effective search space used: 229961028
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)