BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= S06A01NCLL0001_K12 (304 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At5g18380.1 68418.m02162 40S ribosomal protein S16 (RPS16C) 150 2e-37 At2g09990.1 68415.m01037 40S ribosomal protein S16 (RPS16A) Same... 150 2e-37 At3g04230.1 68416.m00447 40S ribosomal protein S16 (RPS16B) simi... 144 8e-36 At3g49080.1 68416.m05362 ribosomal protein S9 family protein con... 33 0.037 At1g11480.1 68414.m01319 eukaryotic translation initiation facto... 29 0.45 At3g24255.1 68416.m03045 expressed protein 29 0.79 At1g04360.1 68414.m00426 zinc finger (C3HC4-type RING finger) fa... 27 1.8 At1g43570.1 68414.m05001 hypothetical protein 27 3.2 At5g48630.1 68418.m06014 cyclin family protein similar to SP|P55... 26 4.2 At2g41680.1 68415.m05149 thioredoxin reductase, putative / NADPH... 26 4.2 At5g18480.1 68418.m02179 glycogenin glucosyltransferase (glycoge... 25 7.4 At3g44240.1 68416.m04747 CCR4-NOT transcription complex protein,... 25 9.7 >At5g18380.1 68418.m02162 40S ribosomal protein S16 (RPS16C) Length = 146 Score = 150 bits (364), Expect = 2e-37 Identities = 67/84 (79%), Positives = 79/84 (94%) Frame = +1 Query: 10 FSGVDIRVTVKGGGHVAQVYAIRQAISKALIAFYQKYVDEASKKEIKDILVQYDRSLLVA 189 F+GV++R+ V GGGH +QVYAIRQ+I+KAL+A+YQKYVDE SKKEIKDILV+YDR+LLVA Sbjct: 63 FAGVNMRIRVNGGGHTSQVYAIRQSIAKALVAYYQKYVDEQSKKEIKDILVRYDRTLLVA 122 Query: 190 DPRRCEPKKFGGPGARARYQKSYR 261 DPRRCEPKKFGG GAR+RYQKSYR Sbjct: 123 DPRRCEPKKFGGRGARSRYQKSYR 146 >At2g09990.1 68415.m01037 40S ribosomal protein S16 (RPS16A) Same as GB:Q42340 Length = 146 Score = 150 bits (364), Expect = 2e-37 Identities = 67/84 (79%), Positives = 79/84 (94%) Frame = +1 Query: 10 FSGVDIRVTVKGGGHVAQVYAIRQAISKALIAFYQKYVDEASKKEIKDILVQYDRSLLVA 189 F+GV++R+ V GGGH +QVYAIRQ+I+KAL+A+YQKYVDE SKKEIKDILV+YDR+LLVA Sbjct: 63 FAGVNMRIRVNGGGHTSQVYAIRQSIAKALVAYYQKYVDEQSKKEIKDILVRYDRTLLVA 122 Query: 190 DPRRCEPKKFGGPGARARYQKSYR 261 DPRRCEPKKFGG GAR+RYQKSYR Sbjct: 123 DPRRCEPKKFGGRGARSRYQKSYR 146 >At3g04230.1 68416.m00447 40S ribosomal protein S16 (RPS16B) similar to 40S ribosomal protein S16 GB:AAD22696 [Arabidopsis thaliana] Length = 146 Score = 144 bits (350), Expect = 8e-36 Identities = 64/84 (76%), Positives = 78/84 (92%) Frame = +1 Query: 10 FSGVDIRVTVKGGGHVAQVYAIRQAISKALIAFYQKYVDEASKKEIKDILVQYDRSLLVA 189 F+GVD+R+ GGG+ ++VYAIRQ+I+KAL+A+YQKYVDE SKKEIKDIL++YDR+LLVA Sbjct: 63 FAGVDMRIRATGGGNTSRVYAIRQSIAKALVAYYQKYVDEQSKKEIKDILMRYDRTLLVA 122 Query: 190 DPRRCEPKKFGGPGARARYQKSYR 261 DPRRCE KKFGGPGARAR+QKSYR Sbjct: 123 DPRRCESKKFGGPGARARFQKSYR 146 >At3g49080.1 68416.m05362 ribosomal protein S9 family protein contains Pfam profile PF00380: ribosomal protein S9 Length = 430 Score = 33.1 bits (72), Expect = 0.037 Identities = 16/26 (61%), Positives = 19/26 (73%) Frame = +1 Query: 22 DIRVTVKGGGHVAQVYAIRQAISKAL 99 DI+ TVKGGG QV AI+ IS+AL Sbjct: 362 DIKCTVKGGGTTGQVGAIQLGISRAL 387 >At1g11480.1 68414.m01319 eukaryotic translation initiation factor-related contains weak similarity to Swiss-Prot:P23588 eukaryotic translation initiation factor 4B (eIF-4B) [Homo sapiens] Length = 578 Score = 29.5 bits (63), Expect = 0.45 Identities = 13/30 (43%), Positives = 19/30 (63%) Frame = -1 Query: 271 NDINGRISGIWHGRLDHRISWARNDEGQRP 182 N ++G S IW+GR + +S A N+ GQ P Sbjct: 146 NSVSGNHSNIWNGRKE--VSVANNEPGQSP 173 >At3g24255.1 68416.m03045 expressed protein Length = 836 Score = 28.7 bits (61), Expect = 0.79 Identities = 18/42 (42%), Positives = 24/42 (57%) Frame = -3 Query: 200 RRGSATSKLLSY*TRMSLISFLDASSTYFW*KAIRALEMACL 75 R G T++ LS+ R+ LIS + S T FW A R L AC+ Sbjct: 142 RIGKWTARHLSFAGRLQLISSVIHSLTNFWMSAFR-LPSACI 182 >At1g04360.1 68414.m00426 zinc finger (C3HC4-type RING finger) family protein contains Pfam profile: PF00097 zinc finger, C3HC4 type (RING finger) Length = 381 Score = 27.5 bits (58), Expect = 1.8 Identities = 11/26 (42%), Positives = 15/26 (57%) Frame = -3 Query: 80 CLIA*TCATCPPPFTVTLISTPENPR 3 C + T +C FT+ LIS P +PR Sbjct: 172 CPLCRTSVSCEASFTLDLISAPSSPR 197 >At1g43570.1 68414.m05001 hypothetical protein Length = 348 Score = 26.6 bits (56), Expect = 3.2 Identities = 15/36 (41%), Positives = 19/36 (52%) Frame = -3 Query: 203 QRRGSATSKLLSY*TRMSLISFLDASSTYFW*KAIR 96 +R S T + LSY R+ LI + S T FW A R Sbjct: 19 KRISSWTGRFLSYCGRLQLIKSVLMSITNFWSSAFR 54 >At5g48630.1 68418.m06014 cyclin family protein similar to SP|P55168 Cyclin C {Gallus gallus}; contains Pfam profile PF00134: Cyclin, N-terminal domain Length = 253 Score = 26.2 bits (55), Expect = 4.2 Identities = 14/32 (43%), Positives = 17/32 (53%), Gaps = 2/32 (6%) Frame = +1 Query: 70 AIRQAISKALIAFYQK--YVDEASKKEIKDIL 159 A + L+ FY K Y DE + EIKDIL Sbjct: 97 AEESVVHAKLLVFYMKKLYADEKFRYEIKDIL 128 >At2g41680.1 68415.m05149 thioredoxin reductase, putative / NADPH-dependent thioredoxin reductase, putative The last 2 exons encode thioredoxin. There is an EST match to exons 5-7, and the distance between exon 7 and exon 8 is only 90bp. It is unlikely this is two separate genes, but more likely a hybrid protein. Length = 529 Score = 26.2 bits (55), Expect = 4.2 Identities = 17/52 (32%), Positives = 28/52 (53%), Gaps = 2/52 (3%) Frame = +1 Query: 31 VTVKGGGHVAQVYAIRQAIS-KALIAFYQKYVDEASKK-EIKDILVQYDRSL 180 VT G G +A + A R S L+ F+Q +EA K+ +D+ ++D +L Sbjct: 374 VTAAGSGCIAALSAERYLTSNNLLVEFHQPQTEEAKKEFTQRDVQEKFDITL 425 >At5g18480.1 68418.m02179 glycogenin glucosyltransferase (glycogenin)-related low similarity to glycogenin-1 from Mus musculus [SP|Q9R062], Rattus norvegicus [SP|O08730], Homo sapiens [SP|P46976]; contains Pfam profile PF01501: Glycosyl transferase family 8 Length = 537 Score = 25.4 bits (53), Expect = 7.4 Identities = 13/46 (28%), Positives = 25/46 (54%) Frame = +1 Query: 88 SKALIAFYQKYVDEASKKEIKDILVQYDRSLLVADPRRCEPKKFGG 225 +K ++A V + SKK +K + ++ L+A+P + P +F G Sbjct: 59 TKDMVALVSDGVSDYSKKLLKADGWKVEKISLLANPNQVHPTRFWG 104 >At3g44240.1 68416.m04747 CCR4-NOT transcription complex protein, putative similar to SWISS-PROT:Q60809 CCR4-NOT transcription complex, subunit 7 (CCR4-associated factor 1, (CAF1) [Mus musculus] Length = 239 Score = 25.0 bits (52), Expect = 9.7 Identities = 12/26 (46%), Positives = 15/26 (57%), Gaps = 1/26 (3%) Frame = -1 Query: 268 DINGRISGIWHGRL-DHRISWARNDE 194 DINGRI G W D + ARN++ Sbjct: 58 DINGRIGGTWEINFSDFGVDDARNEK 83 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 7,211,090 Number of Sequences: 28952 Number of extensions: 132213 Number of successful extensions: 375 Number of sequences better than 10.0: 12 Number of HSP's better than 10.0 without gapping: 368 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 375 length of database: 12,070,560 effective HSP length: 70 effective length of database: 10,043,920 effective search space used: 301317600 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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