BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= S06A01NCLL0001_K08
(350 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC1711.06 |rpl401|rpl4-1, rpl4|60S ribosomal protein L2|Schizo... 141 3e-35
SPBP8B7.03c |rpl402|rpl4-2, rpl4|60S ribosomal protein L2|Schizo... 141 3e-35
SPBC543.09 |||mitochondrial m-AAA protease|Schizosaccharomyces p... 26 2.0
SPBC19C2.11c |||mitochondrial outer membrane protein |Schizosacc... 25 4.5
SPAC23G3.12c |||serine protease |Schizosaccharomyces pombe|chr 1... 24 7.9
SPCC1672.12c |||DUF410 family protein|Schizosaccharomyces pombe|... 24 7.9
SPAC6G10.05c |||TRAPP complex subunit Trs120 |Schizosaccharomyce... 24 7.9
SPBC11G11.05 |rpa34||DNA-directed RNA polymerase I complex subun... 24 7.9
>SPBC1711.06 |rpl401|rpl4-1, rpl4|60S ribosomal protein
L2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 363
Score = 141 bits (341), Expect = 3e-35
Identities = 63/115 (54%), Positives = 86/115 (74%)
Frame = +1
Query: 4 RGVLKVYKSQRLRAGKGKMRNRRRVQRKGPLIIYNKDKGLTRAFRNIPGIEMXXXXXXXX 183
R V+KV S++LRAGKGK+RNRR VQR+GPL+++N+D G+ +AFRNIPG+E+
Sbjct: 178 RDVIKVANSRKLRAGKGKLRNRRHVQRRGPLVVFNEDTGIVKAFRNIPGVEIVNVRRLNL 237
Query: 184 XXXAPGGHLGRFIIWTKSAFDRLDPLFGSWKTPSKEKKNFNLPQPKMANTDLSRL 348
APGGHLGRF+IWTKSAF LD +FGS ++ KKN+ LP+ ++N D++RL
Sbjct: 238 LQLAPGGHLGRFVIWTKSAFGLLDSVFGSTTEVAQLKKNYFLPENIISNADVTRL 292
>SPBP8B7.03c |rpl402|rpl4-2, rpl4|60S ribosomal protein
L2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 363
Score = 141 bits (341), Expect = 3e-35
Identities = 63/115 (54%), Positives = 86/115 (74%)
Frame = +1
Query: 4 RGVLKVYKSQRLRAGKGKMRNRRRVQRKGPLIIYNKDKGLTRAFRNIPGIEMXXXXXXXX 183
R V+KV S++LRAGKGK+RNRR VQR+GPL+++N+D G+ +AFRNIPG+E+
Sbjct: 178 RDVVKVANSRKLRAGKGKLRNRRHVQRRGPLVVFNEDAGIVKAFRNIPGVEIVNVRRLNL 237
Query: 184 XXXAPGGHLGRFIIWTKSAFDRLDPLFGSWKTPSKEKKNFNLPQPKMANTDLSRL 348
APGGHLGRF+IWTKSAF LD +FGS ++ KKN+ LP+ ++N D++RL
Sbjct: 238 LQLAPGGHLGRFVIWTKSAFGLLDSVFGSTTEAAQLKKNYFLPENIISNADVTRL 292
>SPBC543.09 |||mitochondrial m-AAA protease|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 773
Score = 25.8 bits (54), Expect = 2.0
Identities = 16/47 (34%), Positives = 24/47 (51%)
Frame = -3
Query: 222 DEPAEMAAGCQLQEVQLVHVEHLDARDVAECTSQTLVLIIDDKGSFA 82
+E A +AA EVQ+VH E R A ++ VL ++K + A
Sbjct: 513 NEGALIAARSNSNEVQMVHFEQAIERVTAGLEKKSRVLSPEEKNTVA 559
>SPBC19C2.11c |||mitochondrial outer membrane protein
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 452
Score = 24.6 bits (51), Expect = 4.5
Identities = 8/18 (44%), Positives = 14/18 (77%)
Frame = +1
Query: 94 LIIYNKDKGLTRAFRNIP 147
+++++K KG+T FRN P
Sbjct: 134 VLVFSKQKGITVVFRNDP 151
>SPAC23G3.12c |||serine protease |Schizosaccharomyces pombe|chr
1|||Manual
Length = 996
Score = 23.8 bits (49), Expect = 7.9
Identities = 9/24 (37%), Positives = 16/24 (66%)
Frame = +1
Query: 70 RRVQRKGPLIIYNKDKGLTRAFRN 141
R+ ++KG ++ +KDKGL R+
Sbjct: 545 RKSRKKGTALVLDKDKGLAVTSRS 568
>SPCC1672.12c |||DUF410 family protein|Schizosaccharomyces pombe|chr
3|||Manual
Length = 303
Score = 23.8 bits (49), Expect = 7.9
Identities = 15/49 (30%), Positives = 24/49 (48%), Gaps = 8/49 (16%)
Frame = +1
Query: 205 HLGRFIIWTKSAFDRLDP--------LFGSWKTPSKEKKNFNLPQPKMA 327
++ R + W+KSA + D +F WK P+ +K+F L K A
Sbjct: 102 YVKRLLEWSKSAGPQGDKDVHFAVATMFVKWKEPASAEKHFVLGNEKSA 150
>SPAC6G10.05c |||TRAPP complex subunit Trs120 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1210
Score = 23.8 bits (49), Expect = 7.9
Identities = 13/32 (40%), Positives = 18/32 (56%)
Frame = +1
Query: 253 DPLFGSWKTPSKEKKNFNLPQPKMANTDLSRL 348
+PLF + KT S ++ L +PK N L RL
Sbjct: 971 EPLFVTVKTASTDESRSVLIKPKADNVILFRL 1002
>SPBC11G11.05 |rpa34||DNA-directed RNA polymerase I complex subunit
Rpa34 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 251
Score = 23.8 bits (49), Expect = 7.9
Identities = 16/40 (40%), Positives = 21/40 (52%), Gaps = 4/40 (10%)
Frame = -3
Query: 261 QRVKPIESGLGPDDEP-AEMAAGCQ---LQEVQLVHVEHL 154
Q +PI +GP+ EP AE +G + LQE VE L
Sbjct: 173 QHFRPIGDAVGPESEPEAEPKSGIKEHILQETGDATVEEL 212
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,193,721
Number of Sequences: 5004
Number of extensions: 20664
Number of successful extensions: 69
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 66
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 68
length of database: 2,362,478
effective HSP length: 64
effective length of database: 2,042,222
effective search space used: 106195544
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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