BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= S06A01NCLL0001_J24 (530 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2||... 330 6e-92 SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex s... 195 4e-51 SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pomb... 189 3e-49 SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyce... 103 2e-23 SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces po... 94 1e-20 SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Sc... 77 2e-15 SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomy... 39 5e-08 SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe... 44 2e-05 SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit Arp9|Schizosa... 38 0.001 SPAC664.02c |||actin-like protein Arp8 |Schizosaccharomyces pomb... 31 0.14 SPBC21B10.07 |||glycosyl hydrolase family 16|Schizosaccharomyces... 27 1.7 SPAC1834.05 |alg9||mannosyltransferase complex subunit Alg9 |Sch... 27 2.3 SPAC1039.11c ||SPAC922.02c|alpha-glucosidase|Schizosaccharomyces... 25 7.0 SPAC343.16 |lys2||homoaconitate hydratase Lys2|Schizosaccharomyc... 25 9.3 SPAC23H4.13c |||dubious|Schizosaccharomyces pombe|chr 1|||Manual 25 9.3 >SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2|||Manual Length = 375 Score = 330 bits (812), Expect = 6e-92 Identities = 156/173 (90%), Positives = 163/173 (94%) Frame = +1 Query: 10 SLYASGRTTGIVLDSGDGVSHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSF 189 SLYASGRTTGIVLDSGDGV+HTVPIYEGYALPHAI+RLDLAGRDLTDYLMKIL ERGY+F Sbjct: 141 SLYASGRTTGIVLDSGDGVTHTVPIYEGYALPHAIMRLDLAGRDLTDYLMKILMERGYTF 200 Query: 190 TTTAEREIVRDIKEKLCYVALDFEQEMATAASSSSLEKSYELPDGQVITIGNERFRCPEA 369 +TTAEREIVRDIKEKLCYVALDFEQE+ TAA SSSLEKSYELPDGQVITIGNERFR PEA Sbjct: 201 STTAEREIVRDIKEKLCYVALDFEQELQTAAQSSSLEKSYELPDGQVITIGNERFRAPEA 260 Query: 370 LFQPSFLGMEACGIHETTYNSIMKCDVDIRKDLYANTVLSGGTTMYPGIADRM 528 LFQPS LG+E GIHE TYNSIMKCDVDIRKDLY N V+SGGTTMYPGIADRM Sbjct: 261 LFQPSALGLENAGIHEATYNSIMKCDVDIRKDLYGNVVMSGGTTMYPGIADRM 313 >SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex subunit Arp2|Schizosaccharomyces pombe|chr 1|||Manual Length = 390 Score = 195 bits (475), Expect = 4e-51 Identities = 88/173 (50%), Positives = 123/173 (71%) Frame = +1 Query: 10 SLYASGRTTGIVLDSGDGVSHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSF 189 SLYA G ++G+V+DSGDGV+H VP+YE L H + RLD+AGRD T YL+ +L +GY+F Sbjct: 141 SLYAQGLSSGVVVDSGDGVTHIVPVYESVVLNHLVGRLDVAGRDATRYLISLLLRKGYAF 200 Query: 190 TTTAEREIVRDIKEKLCYVALDFEQEMATAASSSSLEKSYELPDGQVITIGNERFRCPEA 369 TA+ E VR++KEKLCYV+ D E + + ++ L ++Y LPDG+VI +G+ER+ CPE Sbjct: 201 NRTADFETVREMKEKLCYVSYDLELDHKLSEETTVLMRNYTLPDGRVIKVGSERYECPEC 260 Query: 370 LFQPSFLGMEACGIHETTYNSIMKCDVDIRKDLYANTVLSGGTTMYPGIADRM 528 LFQP +G E G+ E +++I DVDIRK LY VLSGG++MY G+ R+ Sbjct: 261 LFQPHLVGSEQPGLSEFIFDTIQAADVDIRKYLYRAIVLSGGSSMYAGLPSRL 313 >SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 379 Score = 189 bits (460), Expect = 3e-49 Identities = 85/172 (49%), Positives = 119/172 (69%) Frame = +1 Query: 10 SLYASGRTTGIVLDSGDGVSHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSF 189 +LYAS RTTGIVL+ GDG++H+VPIY+G+++P AI + ++ GRD+TDYL L + G+ Sbjct: 146 ALYASARTTGIVLECGDGLTHSVPIYDGFSIPSAIQQEEIGGRDVTDYLQLQLRKSGHEL 205 Query: 190 TTTAEREIVRDIKEKLCYVALDFEQEMATAASSSSLEKSYELPDGQVITIGNERFRCPEA 369 ++AE+EIVR+IKEK CYVA DF E+ + +Y+LPD Q IT+G E F PE Sbjct: 206 VSSAEKEIVREIKEKCCYVASDFRSEIESWTEHKPQIHTYQLPDNQTITLGTECFSAPEV 265 Query: 370 LFQPSFLGMEACGIHETTYNSIMKCDVDIRKDLYANTVLSGGTTMYPGIADR 525 LF P +G EA G+H + SI+ D+D+R LY+N VLSGG+T+ G +R Sbjct: 266 LFNPEMMGSEASGLHIQLFKSILLSDIDLRSTLYSNIVLSGGSTLLRGFGER 317 >SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyces pombe|chr 1|||Manual Length = 427 Score = 103 bits (247), Expect = 2e-23 Identities = 63/172 (36%), Positives = 91/172 (52%), Gaps = 7/172 (4%) Frame = +1 Query: 34 TGIVLDSGDGVSHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGY--SFTTTAER 207 TG V+DSGDGV+H +P+ EGY + +I + LAGRD+T ++ +L +R S TAER Sbjct: 180 TGTVVDSGDGVTHIIPVAEGYVIGSSIKTMPLAGRDVTYFVQSLLRDRNEPDSSLKTAER 239 Query: 208 EIVRDIKEKLCYVALDFEQEMA--TAASSSSLEKSYELPDGQ--VITIGNERFRCPEALF 375 IKE+ CYV D +E + L+ + E G I +G ERF PE F Sbjct: 240 -----IKEECCYVCPDIVKEFSRFDREPDRYLKYASESITGHSTTIDVGFERFLAPEIFF 294 Query: 376 QPSFLGME-ACGIHETTYNSIMKCDVDIRKDLYANTVLSGGTTMYPGIADRM 528 P + + E N + +D+RK LY N VLSGG+T++ +R+ Sbjct: 295 NPEIASSDFLTPLPELVDNVVQSSPIDVRKGLYKNIVLSGGSTLFKNFGNRL 346 >SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces pombe|chr 3|||Manual Length = 401 Score = 93.9 bits (223), Expect = 1e-20 Identities = 59/183 (32%), Positives = 91/183 (49%), Gaps = 20/183 (10%) Frame = +1 Query: 40 IVLDSGDGVSHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEREIVR 219 +V+DSG +H +P+ + A+ R+D+ G+ LT+YL ++++ R Y+ E IV Sbjct: 159 LVIDSGYSFTHIIPVIDFSVQEQAVKRVDVGGKLLTNYLKEVISYRKYNM--MEETYIVN 216 Query: 220 DIKEKLCYVALDFEQEMATAASS--SSLEKSYELPD------------------GQVITI 339 +IKE +CYV+ +F+++M S LE Y LPD QV+ + Sbjct: 217 EIKESVCYVSQNFKEDMEICHEKPRSKLEICYALPDYSTGKHGYIVRDINQKIEQQVLNL 276 Query: 340 GNERFRCPEALFQPSFLGMEACGIHETTYNSIMKCDVDIRKDLYANTVLSGGTTMYPGIA 519 NERF PE LF PS + + GI E S+ +I+ L N V GG +PG Sbjct: 277 SNERFMIPELLFSPSDIEIREAGIPEAVMQSVTHFPENIQAMLLENVVTIGGNCKFPGFH 336 Query: 520 DRM 528 R+ Sbjct: 337 KRL 339 >SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Schizosaccharomyces pombe|chr 1|||Manual Length = 430 Score = 76.6 bits (180), Expect = 2e-15 Identities = 44/124 (35%), Positives = 67/124 (54%), Gaps = 9/124 (7%) Frame = +1 Query: 184 SFTTTAEREIVRDIKEKLCYVALDFEQEMATAASSSSLEKSYELPDGQVITIGNERFRCP 363 S+ +R ++ + KE + V LD + A A+S + K +E PDG G ERFR Sbjct: 243 SYEIFQKRRVIEEWKESVLDV-LDTPFDEAKASSRNP--KPFEFPDGVTHKFGQERFRIS 299 Query: 364 EALFQPSFLGMEAC---------GIHETTYNSIMKCDVDIRKDLYANTVLSGGTTMYPGI 516 E LF PSF + G+HE Y SI+ CD ++R L N V++GGT++ PG+ Sbjct: 300 EILFNPSFSASRSAETTPPQGSVGLHELVYQSILACDSELRSPLLNNIVVTGGTSLIPGL 359 Query: 517 ADRM 528 ++R+ Sbjct: 360 SERL 363 >SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomyces pombe|chr 2|||Manual Length = 433 Score = 38.7 bits (86), Expect(2) = 5e-08 Identities = 17/41 (41%), Positives = 25/41 (60%) Frame = +1 Query: 406 GIHETTYNSIMKCDVDIRKDLYANTVLSGGTTMYPGIADRM 528 GI + N I +CDVDIR L N ++ GGT++ G + R+ Sbjct: 326 GIPQLFQNCISECDVDIRASLLNNVIVCGGTSLMQGFSLRL 366 Score = 33.1 bits (72), Expect(2) = 5e-08 Identities = 13/35 (37%), Positives = 21/35 (60%) Frame = +1 Query: 277 AASSSSLEKSYELPDGQVITIGNERFRCPEALFQP 381 ++ ++ E +E PDG + G ER++ PE LF P Sbjct: 254 SSETTIAESEFEFPDGSRMMFGAERYQIPEHLFVP 288 Score = 30.7 bits (66), Expect = 0.14 Identities = 15/56 (26%), Positives = 27/56 (48%) Frame = +1 Query: 16 YASGRTTGIVLDSGDGVSHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGY 183 +ASG+ T ++D G S IY+G+ L +G + D L + L ++ + Sbjct: 141 FASGKGTACLVDIGAERSSVSAIYDGFVLQKGYQVQHFSGNAINDILAQTLRDKNF 196 >SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe|chr 2|||Manual Length = 721 Score = 43.6 bits (98), Expect = 2e-05 Identities = 18/61 (29%), Positives = 36/61 (59%) Frame = +1 Query: 346 ERFRCPEALFQPSFLGMEACGIHETTYNSIMKCDVDIRKDLYANTVLSGGTTMYPGIADR 525 E+ R PE +F PS +G++ GI E + + + ++ ++ L +N +++GG PG+ R Sbjct: 594 EQIRVPEVIFSPSIVGIDQAGILEIMRSILQRHSLEEQQKLVSNVLITGGLGSLPGMETR 653 Query: 526 M 528 + Sbjct: 654 I 654 Score = 43.2 bits (97), Expect = 2e-05 Identities = 23/91 (25%), Positives = 42/91 (46%) Frame = +1 Query: 31 TTGIVLDSGDGVSHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAERE 210 ++GIVL+ G+ SH +P+ G + R+ G + YL+K+ + SF Sbjct: 165 SSGIVLNLGNAASHVIPVLNGERILSEAKRISWGGSQSSSYLLKLFQIKYPSFPIKMLPS 224 Query: 211 IVRDIKEKLCYVALDFEQEMATAASSSSLEK 303 + C+V+ D+ ++A A LE+ Sbjct: 225 QAELLMHDHCHVSSDYTHDIAHALDRDILER 255 >SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit Arp9|Schizosaccharomyces pombe|chr 1|||Manual Length = 523 Score = 37.5 bits (83), Expect = 0.001 Identities = 23/73 (31%), Positives = 36/73 (49%) Frame = +1 Query: 13 LYASGRTTGIVLDSGDGVSHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFT 192 LYA G G+V+D G + PI +G + A +L L GR +T +L +L E + Sbjct: 188 LYAIGILHGLVIDIGYEKTDITPILDGQIIFTATQQLPLGGRYMTQHLQNLLRESLPTLK 247 Query: 193 TTAEREIVRDIKE 231 ++ + DI E Sbjct: 248 SSGQYVSKEDITE 260 >SPAC664.02c |||actin-like protein Arp8 |Schizosaccharomyces pombe|chr 1|||Manual Length = 620 Score = 30.7 bits (66), Expect = 0.14 Identities = 13/58 (22%), Positives = 30/58 (51%) Frame = +1 Query: 16 YASGRTTGIVLDSGDGVSHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSF 189 + +G + V+D G + + EG +P++ ++++ G D+T MK+L + + Sbjct: 286 FGAGMSAACVVDMGAQKTSISCVEEGVVVPNSRIKINYGGDDITLLFMKLLMRSHFPY 343 >SPBC21B10.07 |||glycosyl hydrolase family 16|Schizosaccharomyces pombe|chr 2|||Manual Length = 419 Score = 27.1 bits (57), Expect = 1.7 Identities = 19/59 (32%), Positives = 25/59 (42%) Frame = -3 Query: 525 TVGNTRVHGGTTRQYCVGVQVFTDVNVTLHDGVVCRLVDATSFHTQERGLEESLWATEP 349 T+G+ +GG GV V T VTLH G C + D T L+ + W P Sbjct: 227 TLGDDWPNGGEI-DIVEGVNVGTSNQVTLHTGDGCEMEDIKRVMT-GTALQTNCWVDAP 283 >SPAC1834.05 |alg9||mannosyltransferase complex subunit Alg9 |Schizosaccharomyces pombe|chr 1|||Manual Length = 577 Score = 26.6 bits (56), Expect = 2.3 Identities = 12/33 (36%), Positives = 17/33 (51%) Frame = +2 Query: 5 EAHCTRPVVPPVSCWIPAMVYPTQYPSTRVTPY 103 +A P+ WIP MVYP + T+ TP+ Sbjct: 507 KATVNEPIYSKSDGWIPVMVYP--FIDTKQTPF 537 >SPAC1039.11c ||SPAC922.02c|alpha-glucosidase|Schizosaccharomyces pombe|chr 1|||Manual Length = 995 Score = 25.0 bits (52), Expect = 7.0 Identities = 15/37 (40%), Positives = 19/37 (51%) Frame = -3 Query: 453 VNVTLHDGVVCRLVDATSFHTQERGLEESLWATEPFV 343 VN T HDG V + T + Q R +SL + EP V Sbjct: 610 VNATYHDGTVRYNLFNTYGYDQSRVTYDSLTSIEPNV 646 >SPAC343.16 |lys2||homoaconitate hydratase Lys2|Schizosaccharomyces pombe|chr 1|||Manual Length = 721 Score = 24.6 bits (51), Expect = 9.3 Identities = 7/18 (38%), Positives = 13/18 (72%) Frame = +1 Query: 52 SGDGVSHTVPIYEGYALP 105 +G G+ H + + +GYA+P Sbjct: 157 AGRGIGHQIMVEQGYAMP 174 >SPAC23H4.13c |||dubious|Schizosaccharomyces pombe|chr 1|||Manual Length = 184 Score = 24.6 bits (51), Expect = 9.3 Identities = 16/36 (44%), Positives = 19/36 (52%) Frame = +1 Query: 223 IKEKLCYVALDFEQEMATAASSSSLEKSYELPDGQV 330 IKEKL VA EQE + SSL+ S + G V Sbjct: 111 IKEKLRRVANITEQEQQCIPAESSLDLSIQAMKGVV 146 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,188,015 Number of Sequences: 5004 Number of extensions: 42266 Number of successful extensions: 154 Number of sequences better than 10.0: 15 Number of HSP's better than 10.0 without gapping: 140 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 149 length of database: 2,362,478 effective HSP length: 68 effective length of database: 2,022,206 effective search space used: 218398248 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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