Samia BLAST Result against arabidopsis

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= S06A01NCLL0001_J03
         (457 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At1g54220.1 68414.m06182 dihydrolipoamide S-acetyltransferase, p...    78   3e-15
At3g13930.1 68416.m01759 dihydrolipoamide S-acetyltransferase, p...    75   2e-14
At3g52200.1 68416.m05733 dihydrolipoamide S-acetyltransferase, p...    52   1e-07
At2g29210.1 68415.m03550 splicing factor PWI domain-containing p...    27   6.0  

>At1g54220.1 68414.m06182 dihydrolipoamide S-acetyltransferase,
           putative similar to dihydrolipoamide S-acetyltransferase
           GI:5669871 [Zea mays]; contains Pfam profiles PF00198:
           2-oxo acid dehydrogenases acyltransferase (catalytic
           domain), PF00364: Biotin-requiring enzyme, PF02817: e3
           binding domain
          Length = 539

 Score = 77.8 bits (183), Expect = 3e-15
 Identities = 46/140 (32%), Positives = 68/140 (48%), Gaps = 4/140 (2%)
 Frame = +1

Query: 46  DRIYASPFARRIAELKNFRLGG-QGTGLYGSLKSGDLSDXXXXXXXXXXXXXXXXXXXXX 222
           DR++ASP AR++AE  N  L   +GTG  G +   D+ +                     
Sbjct: 245 DRVFASPLARKLAEDNNVPLSDIEGTGPEGRIVKADIDEYLASSGKGATAKPSKSTDSKA 304

Query: 223 T---FVDLPLSGMRETIARRLTAAKQSIPHYQLVATVNVESTLAMRKLVNDKLAAEKSDI 393
               +VD+P S +R+  A RL  +KQ+IPHY L     V+  +A+R  +N    A     
Sbjct: 305 PALDYVDIPHSQIRKVTASRLAFSKQTIPHYYLTVDTCVDKLMALRSQLNSFKEASGGK- 363

Query: 394 NVSVNDLIVNAVAATCKRVP 453
            +SVNDL+V A A   ++VP
Sbjct: 364 RISVNDLVVKAAALALRKVP 383


>At3g13930.1 68416.m01759 dihydrolipoamide S-acetyltransferase,
           putative similar to dihydrolipoamide S-acetyltransferase
           [Zea mays] GI:5669871; contains Pfam profiles PF00198:
           2-oxo acid dehydrogenases acyltransferase (catalytic
           domain), PF00364: Biotin-requiring enzyme, PF02817: e3
           binding domain
          Length = 539

 Score = 75.4 bits (177), Expect = 2e-14
 Identities = 45/140 (32%), Positives = 67/140 (47%), Gaps = 4/140 (2%)
 Frame = +1

Query: 46  DRIYASPFARRIAELKNFRLGG-QGTGLYGSLKSGDLSDXXXXXXXXXXXXXXXXXXX-- 216
           DRI+ASP AR++AE  N  L   +GTG  G +   D+ D                     
Sbjct: 245 DRIFASPLARKLAEDNNVPLSSIKGTGPEGRIVKADVEDFLASGSKETTAKPSKQVDSKV 304

Query: 217 -XXTFVDLPLSGMRETIARRLTAAKQSIPHYQLVATVNVESTLAMRKLVNDKLAAEKSDI 393
               +VD+P + +R+  A RL  +KQ+IPHY L     V+  + +R  +N    A     
Sbjct: 305 PALDYVDIPHTQIRKVTASRLAFSKQTIPHYYLTVDTCVDKMMGLRSQLNSFQEASGGK- 363

Query: 394 NVSVNDLIVNAVAATCKRVP 453
            +SVNDL++ A A   ++VP
Sbjct: 364 RISVNDLVIKAAALALRKVP 383


>At3g52200.1 68416.m05733 dihydrolipoamide S-acetyltransferase,
           putative similar to dihydrolipoamide acetyltransferase
           (E2) subunit of PDC [Arabidopsis thaliana] GI:559395;
           contains Pfam profiles PF00198: 2-oxo acid
           dehydrogenases acyltransferase (catalytic domain),
           PF00364: Biotin-requiring enzyme, PF02817: e3 binding
           domain; supporting cDNA
           gi|5881964|gb|AF066080.1|AF066080
          Length = 637

 Score = 52.4 bits (120), Expect = 1e-07
 Identities = 29/75 (38%), Positives = 43/75 (57%)
 Frame = +1

Query: 226 FVDLPLSGMRETIARRLTAAKQSIPHYQLVATVNVESTLAMRKLVNDKLAAEKSDINVSV 405
           + D P S +R+ IA+RL  +KQ IPH  L + V ++  LA RK +      E   + VSV
Sbjct: 407 YEDFPNSQIRKIIAKRLLESKQKIPHLYLQSDVVLDPLLAFRKELQ-----ENHGVKVSV 461

Query: 406 NDLIVNAVAATCKRV 450
           ND+++ AVA   + V
Sbjct: 462 NDIVIKAVAVALRNV 476


>At2g29210.1 68415.m03550 splicing factor PWI domain-containing
           protein contains Pfam profile PF01480: PWI domain
          Length = 878

 Score = 27.1 bits (57), Expect = 6.0
 Identities = 14/32 (43%), Positives = 19/32 (59%), Gaps = 1/32 (3%)
 Frame = +3

Query: 228 RGLAPERHAGDHR-PSPHSRQAKHPALPARCH 320
           R  +P   A  HR P+P +RQ + P+ PAR H
Sbjct: 329 RRRSPSPPARRHRSPTPPARQRRSPSPPARRH 360


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.317    0.132    0.359 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 7,793,687
Number of Sequences: 28952
Number of extensions: 135319
Number of successful extensions: 538
Number of sequences better than 10.0: 4
Number of HSP's better than 10.0 without gapping: 465
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 532
length of database: 12,070,560
effective HSP length: 75
effective length of database: 9,899,160
effective search space used: 752336160
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)