Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= S06A01NCLL0001_I08
(481 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2||... 297 4e-82
SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex s... 173 9e-45
SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pomb... 158 4e-40
SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyce... 89 4e-19
SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces po... 88 6e-19
SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Sc... 82 4e-17
SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomy... 43 9e-10
SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe... 48 1e-06
SPAC1296.04 |mug65||spore wall assembly protein |Schizosaccharom... 27 1.9
SPAC664.02c |||actin-like protein Arp8 |Schizosaccharomyces pomb... 25 4.5
SPBC29A3.16 |||ribosome biogenesis protein Rrs1 |Schizosaccharom... 25 5.9
SPBC18E5.09c |||sequence orphan|Schizosaccharomyces pombe|chr 2|... 25 7.8
SPAC23H4.13c |||dubious|Schizosaccharomyces pombe|chr 1|||Manual 25 7.8
>SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr
2|||Manual
Length = 375
Score = 297 bits (730), Expect = 4e-82
Identities = 139/157 (88%), Positives = 147/157 (93%)
Frame = +1
Query: 10 SHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEREIVRDIKEKLCYV 189
+HTVPIYEGYALPHAI+RLDLAGRDLTDYLMKIL ERGY+F+TTAEREIVRDIKEKLCYV
Sbjct: 160 THTVPIYEGYALPHAIMRLDLAGRDLTDYLMKILMERGYTFSTTAEREIVRDIKEKLCYV 219
Query: 190 ALDFEQEMATAAASTSLEKSYELPDGQVITIGNERFRCPEALFQPSFLGMESCGIHETVY 369
ALDFEQE+ TAA S+SLEKSYELPDGQVITIGNERFR PEALFQPS LG+E+ GIHE Y
Sbjct: 220 ALDFEQELQTAAQSSSLEKSYELPDGQVITIGNERFRAPEALFQPSALGLENAGIHEATY 279
Query: 370 NSIMKCDVDIRKDLYANTVMSGGTTMYPGIADRMQKE 480
NSIMKCDVDIRKDLY N VMSGGTTMYPGIADRMQKE
Sbjct: 280 NSIMKCDVDIRKDLYGNVVMSGGTTMYPGIADRMQKE 316
>SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex
subunit Arp2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 390
Score = 173 bits (422), Expect = 9e-45
Identities = 77/157 (49%), Positives = 110/157 (70%)
Frame = +1
Query: 10 SHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEREIVRDIKEKLCYV 189
+H VP+YE L H + RLD+AGRD T YL+ +L +GY+F TA+ E VR++KEKLCYV
Sbjct: 160 THIVPVYESVVLNHLVGRLDVAGRDATRYLISLLLRKGYAFNRTADFETVREMKEKLCYV 219
Query: 190 ALDFEQEMATAAASTSLEKSYELPDGQVITIGNERFRCPEALFQPSFLGMESCGIHETVY 369
+ D E + + +T L ++Y LPDG+VI +G+ER+ CPE LFQP +G E G+ E ++
Sbjct: 220 SYDLELDHKLSEETTVLMRNYTLPDGRVIKVGSERYECPECLFQPHLVGSEQPGLSEFIF 279
Query: 370 NSIMKCDVDIRKDLYANTVMSGGTTMYPGIADRMQKE 480
++I DVDIRK LY V+SGG++MY G+ R++KE
Sbjct: 280 DTIQAADVDIRKYLYRAIVLSGGSSMYAGLPSRLEKE 316
>SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 379
Score = 158 bits (384), Expect = 4e-40
Identities = 70/157 (44%), Positives = 104/157 (66%)
Frame = +1
Query: 10 SHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEREIVRDIKEKLCYV 189
+H+VPIY+G+++P AI + ++ GRD+TDYL L + G+ ++AE+EIVR+IKEK CYV
Sbjct: 165 THSVPIYDGFSIPSAIQQEEIGGRDVTDYLQLQLRKSGHELVSSAEKEIVREIKEKCCYV 224
Query: 190 ALDFEQEMATAAASTSLEKSYELPDGQVITIGNERFRCPEALFQPSFLGMESCGIHETVY 369
A DF E+ + +Y+LPD Q IT+G E F PE LF P +G E+ G+H ++
Sbjct: 225 ASDFRSEIESWTEHKPQIHTYQLPDNQTITLGTECFSAPEVLFNPEMMGSEASGLHIQLF 284
Query: 370 NSIMKCDVDIRKDLYANTVMSGGTTMYPGIADRMQKE 480
SI+ D+D+R LY+N V+SGG+T+ G +R E
Sbjct: 285 KSILLSDIDLRSTLYSNIVLSGGSTLLRGFGERFISE 321
>SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 427
Score = 88.6 bits (210), Expect = 4e-19
Identities = 56/164 (34%), Positives = 85/164 (51%), Gaps = 7/164 (4%)
Frame = +1
Query: 10 SHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGY--SFTTTAEREIVRDIKEKLC 183
+H +P+ EGY + +I + LAGRD+T ++ +L +R S TAER IKE+ C
Sbjct: 191 THIIPVAEGYVIGSSIKTMPLAGRDVTYFVQSLLRDRNEPDSSLKTAER-----IKEECC 245
Query: 184 YVALDFEQEMATAAASTS--LEKSYELPDGQVITI--GNERFRCPEALFQPSFLGMESCG 351
YV D +E + L+ + E G TI G ERF PE F P +
Sbjct: 246 YVCPDIVKEFSRFDREPDRYLKYASESITGHSTTIDVGFERFLAPEIFFNPEIASSDFLT 305
Query: 352 -IHETVYNSIMKCDVDIRKDLYANTVMSGGTTMYPGIADRMQKE 480
+ E V N + +D+RK LY N V+SGG+T++ +R+Q++
Sbjct: 306 PLPELVDNVVQSSPIDVRKGLYKNIVLSGGSTLFKNFGNRLQRD 349
>SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 401
Score = 88.2 bits (209), Expect = 6e-19
Identities = 56/177 (31%), Positives = 87/177 (49%), Gaps = 20/177 (11%)
Frame = +1
Query: 10 SHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEREIVRDIKEKLCYV 189
+H +P+ + A+ R+D+ G+ LT+YL ++++ R Y+ E IV +IKE +CYV
Sbjct: 168 THIIPVIDFSVQEQAVKRVDVGGKLLTNYLKEVISYRKYNM--MEETYIVNEIKESVCYV 225
Query: 190 ALDFEQEMATA--AASTSLEKSYELPD------------------GQVITIGNERFRCPE 309
+ +F+++M + LE Y LPD QV+ + NERF PE
Sbjct: 226 SQNFKEDMEICHEKPRSKLEICYALPDYSTGKHGYIVRDINQKIEQQVLNLSNERFMIPE 285
Query: 310 ALFQPSFLGMESCGIHETVYNSIMKCDVDIRKDLYANTVMSGGTTMYPGIADRMQKE 480
LF PS + + GI E V S+ +I+ L N V GG +PG R+ E
Sbjct: 286 LLFSPSDIEIREAGIPEAVMQSVTHFPENIQAMLLENVVTIGGNCKFPGFHKRLSSE 342
>SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit
Arp42|Schizosaccharomyces pombe|chr 1|||Manual
Length = 430
Score = 82.2 bits (194), Expect = 4e-17
Identities = 46/127 (36%), Positives = 67/127 (52%), Gaps = 9/127 (7%)
Frame = +1
Query: 127 SFTTTAEREIVRDIKEKLCYVALDFEQEMATAAASTSLEKSYELPDGQVITIGNERFRCP 306
S+ +R ++ + KE + V + A AS+ K +E PDG G ERFR
Sbjct: 243 SYEIFQKRRVIEEWKESVLDV---LDTPFDEAKASSRNPKPFEFPDGVTHKFGQERFRIS 299
Query: 307 EALFQPSFLGMESC---------GIHETVYNSIMKCDVDIRKDLYANTVMSGGTTMYPGI 459
E LF PSF S G+HE VY SI+ CD ++R L N V++GGT++ PG+
Sbjct: 300 EILFNPSFSASRSAETTPPQGSVGLHELVYQSILACDSELRSPLLNNIVVTGGTSLIPGL 359
Query: 460 ADRMQKE 480
++R+Q E
Sbjct: 360 SERLQAE 366
>SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 433
Score = 43.2 bits (97), Expect(2) = 9e-10
Identities = 19/44 (43%), Positives = 27/44 (61%)
Frame = +1
Query: 349 GIHETVYNSIMKCDVDIRKDLYANTVMSGGTTMYPGIADRMQKE 480
GI + N I +CDVDIR L N ++ GGT++ G + R+Q E
Sbjct: 326 GIPQLFQNCISECDVDIRASLLNNVIVCGGTSLMQGFSLRLQNE 369
Score = 34.3 bits (75), Expect(2) = 9e-10
Identities = 14/35 (40%), Positives = 21/35 (60%)
Frame = +1
Query: 220 AAASTSLEKSYELPDGQVITIGNERFRCPEALFQP 324
++ +T E +E PDG + G ER++ PE LF P
Sbjct: 254 SSETTIAESEFEFPDGSRMMFGAERYQIPEHLFVP 288
>SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 721
Score = 47.6 bits (108), Expect = 1e-06
Identities = 19/64 (29%), Positives = 40/64 (62%)
Frame = +1
Query: 289 ERFRCPEALFQPSFLGMESCGIHETVYNSIMKCDVDIRKDLYANTVMSGGTTMYPGIADR 468
E+ R PE +F PS +G++ GI E + + + + ++ ++ L +N +++GG PG+ R
Sbjct: 594 EQIRVPEVIFSPSIVGIDQAGILEIMRSILQRHSLEEQQKLVSNVLITGGLGSLPGMETR 653
Query: 469 MQKE 480
+++E
Sbjct: 654 IKRE 657
Score = 31.5 bits (68), Expect = 0.068
Identities = 18/79 (22%), Positives = 33/79 (41%)
Frame = +1
Query: 10 SHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEREIVRDIKEKLCYV 189
SH +P+ G + R+ G + YL+K+ + SF + C+V
Sbjct: 177 SHVIPVLNGERILSEAKRISWGGSQSSSYLLKLFQIKYPSFPIKMLPSQAELLMHDHCHV 236
Query: 190 ALDFEQEMATAAASTSLEK 246
+ D+ ++A A LE+
Sbjct: 237 SSDYTHDIAHALDRDILER 255
>SPAC1296.04 |mug65||spore wall assembly protein
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 248
Score = 26.6 bits (56), Expect = 1.9
Identities = 9/16 (56%), Positives = 9/16 (56%)
Frame = +2
Query: 26 STKVTPCPTPSSVWTW 73
STK CP PS W W
Sbjct: 52 STKTATCPDPSWEWAW 67
>SPAC664.02c |||actin-like protein Arp8 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 620
Score = 25.4 bits (53), Expect = 4.5
Identities = 9/36 (25%), Positives = 21/36 (58%)
Frame = +1
Query: 25 IYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSF 132
+ EG +P++ ++++ G D+T MK+L + +
Sbjct: 308 VEEGVVVPNSRIKINYGGDDITLLFMKLLMRSHFPY 343
>SPBC29A3.16 |||ribosome biogenesis protein Rrs1
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 166
Score = 25.0 bits (52), Expect = 5.9
Identities = 9/21 (42%), Positives = 11/21 (52%)
Frame = +2
Query: 5 EALTPCPSTKVTPCPTPSSVW 67
E +TP P K P P P + W
Sbjct: 73 ETVTPLPRAKPLPKPKPETKW 93
>SPBC18E5.09c |||sequence orphan|Schizosaccharomyces pombe|chr
2|||Manual
Length = 128
Score = 24.6 bits (51), Expect = 7.8
Identities = 13/43 (30%), Positives = 21/43 (48%), Gaps = 1/43 (2%)
Frame = +1
Query: 181 CYVALDFEQEMATAAASTSLEKSYELPDGQVITIGNE-RFRCP 306
CYV+ +E + + SL SY +PD + I + R+ P
Sbjct: 39 CYVSRPLTKERSCSHPYISLGSSYGIPDAENIEYPRDARYHSP 81
>SPAC23H4.13c |||dubious|Schizosaccharomyces pombe|chr 1|||Manual
Length = 184
Score = 24.6 bits (51), Expect = 7.8
Identities = 16/36 (44%), Positives = 19/36 (52%)
Frame = +1
Query: 166 IKEKLCYVALDFEQEMATAAASTSLEKSYELPDGQV 273
IKEKL VA EQE A +SL+ S + G V
Sbjct: 111 IKEKLRRVANITEQEQQCIPAESSLDLSIQAMKGVV 146
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,906,001
Number of Sequences: 5004
Number of extensions: 34492
Number of successful extensions: 134
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 121
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 129
length of database: 2,362,478
effective HSP length: 68
effective length of database: 2,022,206
effective search space used: 184020746
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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