Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= S06A01NCLL0001_H14
(512 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g13950.1 68414.m01639 eukaryotic translation initiation facto... 198 2e-51
At1g69410.1 68414.m07972 eukaryotic translation initiation facto... 187 4e-48
At1g26630.1 68414.m03243 eukaryotic translation initiation facto... 185 1e-47
At3g26570.2 68416.m03317 phosphate transporter family protein co... 31 0.60
At3g26570.1 68416.m03316 phosphate transporter family protein co... 31 0.60
At3g46960.1 68416.m05099 DEAD/DEAH box helicase, putative simila... 29 1.8
At3g05830.1 68416.m00654 expressed protein 28 3.2
At5g03140.1 68418.m00262 lectin protein kinase family protein co... 28 4.2
At2g15860.1 68415.m01818 expressed protein and genefinder 28 4.2
At5g14230.1 68418.m01663 ankyrin repeat family protein contains ... 27 7.4
At3g48770.1 68416.m05326 hypothetical protein 27 7.4
At2g27680.1 68415.m03354 aldo/keto reductase family protein cont... 27 7.4
At5g17190.1 68418.m02014 expressed protein similar to unknown pr... 27 9.8
At3g19490.1 68416.m02470 sodium hydrogen antiporter, putative si... 27 9.8
At1g79920.2 68414.m09339 heat shock protein 70, putative / HSP70... 27 9.8
At1g79920.1 68414.m09338 heat shock protein 70, putative / HSP70... 27 9.8
At1g66120.1 68414.m07504 acyl-activating enzyme 11 (AAE11) simil... 27 9.8
At1g19485.1 68414.m02427 AT hook motif-containing protein contai... 27 9.8
>At1g13950.1 68414.m01639 eukaryotic translation initiation factor
5A-1 / eIF-5A 1 identical to SP|Q9XI91 Eukaryotic
translation initiation factor 5A-1 (eIF-5A 1)
{Arabidopsis thaliana}
Length = 158
Score = 198 bits (483), Expect = 2e-51
Identities = 85/144 (59%), Positives = 114/144 (79%), Gaps = 1/144 (0%)
Frame = +3
Query: 75 EDTHFETGDSGASATFPMQCSALRKNGFVMLKSRPCKIVEMSTSKTGKHGHAKVHLVGID 254
E+ HFE+ D+GAS T+P Q +RKNG++++K+RPCK+VE+STSKTGKHGHAK H V ID
Sbjct: 4 EEHHFESSDAGASKTYPQQAGTIRKNGYIVIKNRPCKVVEVSTSKTGKHGHAKCHFVAID 63
Query: 255 IFNGKKYEDICPSTHNMDVPHVKREDYQLTDISDDGYLTLMADNGDLREDLKIPDGD-LG 431
IF KK EDI PS+HN DVPHV R DYQL DIS+DGY++L+ DNG ++DLK+P+ D L
Sbjct: 64 IFTSKKLEDIVPSSHNCDVPHVNRTDYQLIDISEDGYVSLLTDNGSTKDDLKLPNDDTLL 123
Query: 432 VQLRTDFDSGKELLCTVLKSCGEE 503
Q+++ FD GK+L+ +V+ + GEE
Sbjct: 124 QQIKSGFDDGKDLVVSVMSAMGEE 147
>At1g69410.1 68414.m07972 eukaryotic translation initiation factor
5A, putative / eIF-5A, putative strong similarity to
eukaryotic initiation factor 5A (2) (Nicotiana
plumbaginifolia) GI:19702, SP|Q9AXQ6| Eukaryotic
translation initiation factor 5A-1 (eIF-5A 1)
{Lycopersicon esculentum}
Length = 158
Score = 187 bits (455), Expect = 4e-48
Identities = 81/144 (56%), Positives = 109/144 (75%), Gaps = 1/144 (0%)
Frame = +3
Query: 75 EDTHFETGDSGASATFPMQCSALRKNGFVMLKSRPCKIVEMSTSKTGKHGHAKVHLVGID 254
++ HFE+ D+GAS T+P Q +RK G +++K RPCK+VE+STSKTGKHGHAK H V ID
Sbjct: 4 DEHHFESSDAGASKTYPQQAGNIRKGGHIVIKGRPCKVVEVSTSKTGKHGHAKCHFVAID 63
Query: 255 IFNGKKYEDICPSTHNMDVPHVKREDYQLTDISDDGYLTLMADNGDLREDLKIP-DGDLG 431
IF KK EDI PS+HN DVPHV R DYQL DIS+DG+++L+ DNG ++DLK+P D L
Sbjct: 64 IFTSKKLEDIVPSSHNCDVPHVNRVDYQLIDISEDGFVSLLTDNGSTKDDLKLPTDEALL 123
Query: 432 VQLRTDFDSGKELLCTVLKSCGEE 503
QL+ F+ GK+++ +V+ + GEE
Sbjct: 124 TQLKNGFEEGKDIVVSVMSAMGEE 147
>At1g26630.1 68414.m03243 eukaryotic translation initiation factor
5A, putative / eIF-5A, putative strong similariy to
SP|Q9AXQ6 Eukaryotic translation initiation factor 5A-1
(eIF-5A 1) {Lycopersicon esculentum}
Length = 159
Score = 185 bits (451), Expect = 1e-47
Identities = 80/144 (55%), Positives = 108/144 (75%), Gaps = 1/144 (0%)
Frame = +3
Query: 75 EDTHFETGDSGASATFPMQCSALRKNGFVMLKSRPCKIVEMSTSKTGKHGHAKVHLVGID 254
++ HFE +SGAS T+P +RK G +++K+RPCK+VE+STSKTGKHGHAK H V ID
Sbjct: 4 DEHHFEASESGASKTYPQSAGNIRKGGHIVIKNRPCKVVEVSTSKTGKHGHAKCHFVAID 63
Query: 255 IFNGKKYEDICPSTHNMDVPHVKREDYQLTDISDDGYLTLMADNGDLREDLKIP-DGDLG 431
IF KK EDI PS+HN DVPHV R DYQL DI++DG+++L+ D+G ++DLK+P D L
Sbjct: 64 IFTAKKLEDIVPSSHNCDVPHVNRVDYQLIDITEDGFVSLLTDSGGTKDDLKLPTDDGLT 123
Query: 432 VQLRTDFDSGKELLCTVLKSCGEE 503
Q+R FD GK+++ +V+ S GEE
Sbjct: 124 AQMRLGFDEGKDIVVSVMSSMGEE 147
>At3g26570.2 68416.m03317 phosphate transporter family protein
contains Pfam profile: PF01384 phosphate transporter
family
Length = 587
Score = 30.7 bits (66), Expect = 0.60
Identities = 17/37 (45%), Positives = 22/37 (59%)
Frame = -2
Query: 496 PQDFSTVHSNSLPLSKSVRSCTPRSPSGILRSSRRSP 386
P FS+V ++SL L K+ CTPRS G S + SP
Sbjct: 4 PYRFSSVRNHSL-LLKTSHLCTPRSALGCCFSPKESP 39
>At3g26570.1 68416.m03316 phosphate transporter family protein
contains Pfam profile: PF01384 phosphate transporter
family
Length = 613
Score = 30.7 bits (66), Expect = 0.60
Identities = 17/37 (45%), Positives = 22/37 (59%)
Frame = -2
Query: 496 PQDFSTVHSNSLPLSKSVRSCTPRSPSGILRSSRRSP 386
P FS+V ++SL L K+ CTPRS G S + SP
Sbjct: 30 PYRFSSVRNHSL-LLKTSHLCTPRSALGCCFSPKESP 65
>At3g46960.1 68416.m05099 DEAD/DEAH box helicase, putative similar to
SP|P35207 Antiviral protein SKI2 {Saccharomyces
cerevisiae}; contains Pfam profiles PF00270: DEAD/DEAH
box helicase, PF00271: Helicase conserved C-terminal
domain
Length = 1347
Score = 29.1 bits (62), Expect = 1.8
Identities = 18/51 (35%), Positives = 31/51 (60%), Gaps = 6/51 (11%)
Frame = +3
Query: 348 ISDDGYLTLMADNG--DLREDLKIPDGDLGVQLR----TDFDSGKELLCTV 482
+SD+ L + A G D+ +++ D DL VQ++ + +SG+EL+CTV
Sbjct: 1141 MSDEALLQMPAFQGRIDVLKNIGCIDDDLVVQIKGRVACEMNSGEELICTV 1191
>At3g05830.1 68416.m00654 expressed protein
Length = 336
Score = 28.3 bits (60), Expect = 3.2
Identities = 24/82 (29%), Positives = 39/82 (47%)
Frame = +3
Query: 228 AKVHLVGIDIFNGKKYEDICPSTHNMDVPHVKREDYQLTDISDDGYLTLMADNGDLREDL 407
AK + G+D K ED+ P V+ +D ++T + D+ + LM +G +
Sbjct: 186 AKAGIGGMDSELQKLLEDVSPMKFERMNRLVEVKDEEITKLKDE--IRLM--SGQWKHKT 241
Query: 408 KIPDGDLGVQLRTDFDSGKELL 473
K + L Q RTD D K++L
Sbjct: 242 KELESQLEKQRRTDQDLKKKVL 263
>At5g03140.1 68418.m00262 lectin protein kinase family protein
contains Pfam domains, PF00138: Legume lectins alpha
domain, PF00139: Legume lectins beta domain and PF00069:
Protein kinase domain
Length = 711
Score = 27.9 bits (59), Expect = 4.2
Identities = 12/32 (37%), Positives = 19/32 (59%)
Frame = -2
Query: 163 ITKPFLRRAEHCMGNVAEAPESPVSKCVSSMS 68
+T+P +R + A+ PE P++K SSMS
Sbjct: 635 VTRPTMRSVVQILVGEADVPEVPIAKPSSSMS 666
>At2g15860.1 68415.m01818 expressed protein and genefinder
Length = 512
Score = 27.9 bits (59), Expect = 4.2
Identities = 22/62 (35%), Positives = 29/62 (46%), Gaps = 1/62 (1%)
Frame = -1
Query: 236 NLSVTVFASFGSGHLDNLAGATLQHYETVLTQGGALHGKCCRGTGVSGL-EMCIFNVTHC 60
N SV+ A +G + LAG L + + GG L GV L EMC F VTH
Sbjct: 328 NSSVSKAADMATGFTNALAG--LHVNDMIQRTGGRLES--LHSEGVHRLSEMCCFAVTHL 383
Query: 59 VV 54
++
Sbjct: 384 LI 385
>At5g14230.1 68418.m01663 ankyrin repeat family protein contains
ankyrin repeats, Pfam:PF00023
Length = 591
Score = 27.1 bits (57), Expect = 7.4
Identities = 14/34 (41%), Positives = 15/34 (44%)
Frame = +3
Query: 405 LKIPDGDLGVQLRTDFDSGKELLCTVLKSCGEEC 506
L IPDGD L G +C L SCG C
Sbjct: 412 LDIPDGDGYTPLMLAAREGHGHMCEYLISCGANC 445
>At3g48770.1 68416.m05326 hypothetical protein
Length = 1899
Score = 27.1 bits (57), Expect = 7.4
Identities = 17/48 (35%), Positives = 27/48 (56%)
Frame = +3
Query: 6 RPSVTYHSLP*LKLSTHNTMGDIEDTHFETGDSGASATFPMQCSALRK 149
R S+ H L KLST N++G + +T+F T S + ++ + SA K
Sbjct: 414 RLSIREHCLD-PKLSTVNSIGIVSETNFVTRKSIDAESYTIHLSASEK 460
>At2g27680.1 68415.m03354 aldo/keto reductase family protein
contains Pfam profile PF00248: oxidoreductase, aldo/keto
reductase family
Length = 384
Score = 27.1 bits (57), Expect = 7.4
Identities = 15/46 (32%), Positives = 24/46 (52%)
Frame = +3
Query: 276 EDICPSTHNMDVPHVKREDYQLTDISDDGYLTLMADNGDLREDLKI 413
++I S MDV + + D ++DGYL + DL+E+ KI
Sbjct: 147 QNIDISRKRMDVAALDMLQFHWWDYANDGYLDALKHLTDLKEEGKI 192
>At5g17190.1 68418.m02014 expressed protein similar to unknown
protein (gb|AAF26109.1)
Length = 130
Score = 26.6 bits (56), Expect = 9.8
Identities = 9/16 (56%), Positives = 12/16 (75%)
Frame = -3
Query: 210 FWKWTSRQSCRGDSST 163
+WK+ +R SC GDS T
Sbjct: 60 YWKYETRPSCDGDSCT 75
>At3g19490.1 68416.m02470 sodium hydrogen antiporter, putative
similar to NhaD [Vibrio parahaemolyticus]
gi|3123728|dbj|BAA25994; Na+/H+ aniporter (NhaD) family
member, PMID:11500563
Length = 576
Score = 26.6 bits (56), Expect = 9.8
Identities = 12/30 (40%), Positives = 20/30 (66%)
Frame = -2
Query: 310 TSMLWVEGQISSYFLPLNISMPTK*TLA*P 221
T+MLW+ GQIS+ ++ +P+ +LA P
Sbjct: 301 TTMLWIHGQISTLPTMKDLFLPSVVSLAVP 330
>At1g79920.2 68414.m09339 heat shock protein 70, putative / HSP70,
putative contains Pfam profile: PF00012 Heat shock hsp70
proteins; similar to heat-shock proteins GB:CAA94389,
GB:AAD55461 [Arabidopsis thaliana]
Length = 736
Score = 26.6 bits (56), Expect = 9.8
Identities = 13/43 (30%), Positives = 22/43 (51%), Gaps = 1/43 (2%)
Frame = +1
Query: 355 TTATSPSWLTTVTCVKTSRYRTAISAYNCVPTLTAA-RSYCAL 480
++ +S W+T + CV SR++ S + LTA R C +
Sbjct: 679 SSRSSRRWVTLLKCVTRSRWKEGRSLISLATVLTATERQLCLM 721
>At1g79920.1 68414.m09338 heat shock protein 70, putative / HSP70,
putative contains Pfam profile: PF00012 Heat shock hsp70
proteins; similar to heat-shock proteins GB:CAA94389,
GB:AAD55461 [Arabidopsis thaliana]
Length = 736
Score = 26.6 bits (56), Expect = 9.8
Identities = 13/43 (30%), Positives = 22/43 (51%), Gaps = 1/43 (2%)
Frame = +1
Query: 355 TTATSPSWLTTVTCVKTSRYRTAISAYNCVPTLTAA-RSYCAL 480
++ +S W+T + CV SR++ S + LTA R C +
Sbjct: 679 SSRSSRRWVTLLKCVTRSRWKEGRSLISLATVLTATERQLCLM 721
>At1g66120.1 68414.m07504 acyl-activating enzyme 11 (AAE11) similar
to AMP-binding protein GI:1903034 from [Brassica napus];
contains Pfam AMP-binding domain PF00501; identical to
cDNA acyl-activating enzyme 11 (At1g66120) GI:29893230,
acyl-activating enzyme 11 [Arabidopsis thaliana]
GI:29893231
Length = 572
Score = 26.6 bits (56), Expect = 9.8
Identities = 15/45 (33%), Positives = 23/45 (51%)
Frame = -2
Query: 508 THSSPQDFSTVHSNSLPLSKSVRSCTPRSPSGILRSSRRSPLSAM 374
T SS VH+ P+S + S T P G++ S + + LSA+
Sbjct: 172 TPSSSASMFRVHNEHDPISLNYTSGTTADPKGVVISHQGAYLSAL 216
>At1g19485.1 68414.m02427 AT hook motif-containing protein contains
Pfam profile: PF00730 HhH-GPD superfamily base excision
DNA repair protein; contains Pfam PF02178: AT hook
motif; contains Pfam PF00400: WD domain, G-beta repeat
(5 copies); contains Prosite PS00354: HMG-I and HMG-Y
DNA-binding domain (A+T-hook)
Length = 815
Score = 26.6 bits (56), Expect = 9.8
Identities = 10/35 (28%), Positives = 19/35 (54%)
Frame = -2
Query: 154 PFLRRAEHCMGNVAEAPESPVSKCVSSMSPIVLCV 50
P + +GNV P S +S+ + ++ +VLC+
Sbjct: 318 PMILEYSEAVGNVPSKPSSGISEDIVALPRVVLCL 352
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 11,849,414
Number of Sequences: 28952
Number of extensions: 258041
Number of successful extensions: 747
Number of sequences better than 10.0: 18
Number of HSP's better than 10.0 without gapping: 721
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 744
length of database: 12,070,560
effective HSP length: 76
effective length of database: 9,870,208
effective search space used: 927799552
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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