BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= S06A01NCLL0001_G07 (461 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At4g26540.1 68417.m03823 protein kinase family protein Three fal... 27 8.2 At4g13420.1 68417.m02095 potassium transporter (HAK5) identical ... 27 8.2 >At4g26540.1 68417.m03823 protein kinase family protein Three false introns were added with non-consensus splice sites to circumenvent frameshifts likely due to sequencing errors; this is extremely unusual and is under investigation. Length = 1089 Score = 26.6 bits (56), Expect = 8.2 Identities = 13/32 (40%), Positives = 20/32 (62%) Frame = +2 Query: 116 SSTVFRLTILILTFIVTCTFIASVVWLLQTRA 211 +S+V RLTILIL + + +V L++ RA Sbjct: 695 NSSVVRLTILILVVVTAVLVLMAVYTLVRARA 726 >At4g13420.1 68417.m02095 potassium transporter (HAK5) identical to K+ transporter HAK5 [Arabidopsis thaliana] gi|7108597|gb|AAF36490; similar to high-affinity potassium transporter AtKUP1p [Arabidopsis thaliana] gi|2688979|gb|AAB88901; KUP/HAK/KT Transporter family member, PMID:11500563 Length = 785 Score = 26.6 bits (56), Expect = 8.2 Identities = 13/43 (30%), Positives = 22/43 (51%) Frame = +2 Query: 122 TVFRLTILILTFIVTCTFIASVVWLLQTRANVGQYENYYLSRI 250 TV +T L++T I+ + ++VW+ G E YLS + Sbjct: 456 TVMVITTLMVTLIMLVIWKTNIVWIAIFLVVFGSIEMLYLSSV 498 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 8,017,155 Number of Sequences: 28952 Number of extensions: 128978 Number of successful extensions: 347 Number of sequences better than 10.0: 2 Number of HSP's better than 10.0 without gapping: 346 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 347 length of database: 12,070,560 effective HSP length: 75 effective length of database: 9,899,160 effective search space used: 772134480 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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