Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= S06A01NCLL0001_E21
(420 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g52640.1 68418.m06535 heat shock protein 81-1 (HSP81-1) / hea... 169 7e-43
At5g56030.1 68418.m06991 heat shock protein 81-2 (HSP81-2) nearl... 163 6e-41
At5g56010.1 68418.m06989 heat shock protein, putative strong sim... 163 6e-41
At5g56000.1 68418.m06988 heat shock protein 81-4 (HSP81-4) nearl... 161 1e-40
At4g24190.2 68417.m03473 shepherd protein (SHD) / clavata format... 103 4e-23
At4g24190.1 68417.m03472 shepherd protein (SHD) / clavata format... 103 4e-23
At3g07770.1 68416.m00947 heat shock protein-related strong simil... 93 7e-20
At2g04030.2 68415.m00372 heat shock protein, putative strong sim... 91 2e-19
At2g04030.1 68415.m00371 heat shock protein, putative strong sim... 91 2e-19
At3g17620.1 68416.m02251 F-box family protein contains Pfam prof... 28 2.2
At3g13530.1 68416.m01701 MAP3K epsilon protein kinase identical ... 28 3.0
At2g01520.1 68415.m00076 major latex protein-related / MLP-relat... 28 3.0
At5g60840.1 68418.m07632 expressed protein predicted protein, Dr... 27 3.9
At3g07980.1 68416.m00975 protein kinase, putative similar to MAP... 27 6.8
At5g03650.1 68418.m00324 1,4-alpha-glucan branching enzyme / sta... 26 9.0
At1g75470.1 68414.m08766 purine permease-related contains Pfam p... 26 9.0
At1g14930.1 68414.m01784 major latex protein-related / MLP-relat... 26 9.0
>At5g52640.1 68418.m06535 heat shock protein 81-1 (HSP81-1) / heat
shock protein 83 (HSP83) nearly identical to SP|P27323
Heat shock protein 81-1 (HSP81-1) (Heat shock protein
83) {Arabidopsis thaliana}; contains Pfam profiles
PF02518: ATPase, histidine kinase-, DNA gyrase B-, and
HSP90-like domain protein, PF00183: Hsp90 protein
Length = 705
Score = 169 bits (411), Expect = 7e-43
Identities = 73/94 (77%), Positives = 85/94 (90%)
Frame = +2
Query: 137 LNKTKPIWTRNADDITQEEYGDFYKSLTNDWEDHLAVKHFSVEGQLEFRALLFVPRRAPF 316
+NK KPIW R ++IT+EEY FYKSLTNDWEDHLAVKHFSVEGQLEF+A+LFVP+RAPF
Sbjct: 267 INKQKPIWLRKPEEITKEEYAAFYKSLTNDWEDHLAVKHFSVEGQLEFKAILFVPKRAPF 326
Query: 317 DLFENKRRKNKIKLYVRRVFIMDNCEELIPEYLN 418
DLF+ +++ N IKLYVRRVFIMDNCEELIPEYL+
Sbjct: 327 DLFDTRKKLNNIKLYVRRVFIMDNCEELIPEYLS 360
>At5g56030.1 68418.m06991 heat shock protein 81-2 (HSP81-2) nearly
identical to SP|P55737 Heat shock protein 81-2 (HSP81-2)
{Arabidopsis thaliana}
Length = 699
Score = 163 bits (395), Expect = 6e-41
Identities = 69/93 (74%), Positives = 83/93 (89%)
Frame = +2
Query: 137 LNKTKPIWTRNADDITQEEYGDFYKSLTNDWEDHLAVKHFSVEGQLEFRALLFVPRRAPF 316
+NK KPIW R ++I +EEY FYKSL+NDWE+HLAVKHFSVEGQLEF+A+LFVP+RAPF
Sbjct: 261 VNKQKPIWMRKPEEINKEEYAAFYKSLSNDWEEHLAVKHFSVEGQLEFKAILFVPKRAPF 320
Query: 317 DLFENKRRKNKIKLYVRRVFIMDNCEELIPEYL 415
DLF+ K++ N IKLYVRRVFIMDNCE++IPEYL
Sbjct: 321 DLFDTKKKPNNIKLYVRRVFIMDNCEDIIPEYL 353
>At5g56010.1 68418.m06989 heat shock protein, putative strong
similarity to SP|P55737 Heat shock protein 81-2
(HSP81-2) {Arabidopsis thaliana}; contains Pfam profiles
PF02518: ATPase, histidine kinase-, DNA gyrase B-, and
HSP90-like domain protein, PF00183: Hsp90 protein
Length = 699
Score = 163 bits (395), Expect = 6e-41
Identities = 69/93 (74%), Positives = 83/93 (89%)
Frame = +2
Query: 137 LNKTKPIWTRNADDITQEEYGDFYKSLTNDWEDHLAVKHFSVEGQLEFRALLFVPRRAPF 316
+NK KPIW R ++I +EEY FYKSL+NDWE+HLAVKHFSVEGQLEF+A+LFVP+RAPF
Sbjct: 261 VNKQKPIWMRKPEEINKEEYAAFYKSLSNDWEEHLAVKHFSVEGQLEFKAILFVPKRAPF 320
Query: 317 DLFENKRRKNKIKLYVRRVFIMDNCEELIPEYL 415
DLF+ K++ N IKLYVRRVFIMDNCE++IPEYL
Sbjct: 321 DLFDTKKKPNNIKLYVRRVFIMDNCEDIIPEYL 353
>At5g56000.1 68418.m06988 heat shock protein 81-4 (HSP81-4) nearly
identical to heat shock protein hsp81.4 [Arabidopsis
thaliana] GI:1906828; contains Pfam profiles PF02518:
ATPase, histidine kinase-, DNA gyrase B-, and HSP90-like
domain protein, PF00183: Hsp90 protein
Length = 699
Score = 161 bits (392), Expect = 1e-40
Identities = 68/93 (73%), Positives = 83/93 (89%)
Frame = +2
Query: 137 LNKTKPIWTRNADDITQEEYGDFYKSLTNDWEDHLAVKHFSVEGQLEFRALLFVPRRAPF 316
+NK KPIW R ++I +EEY FYKSL+NDWE+HLAVKHFSVEGQLEF+A+LFVP+RAPF
Sbjct: 261 VNKQKPIWMRKPEEINKEEYAAFYKSLSNDWEEHLAVKHFSVEGQLEFKAILFVPKRAPF 320
Query: 317 DLFENKRRKNKIKLYVRRVFIMDNCEELIPEYL 415
DLF+ K++ N IKLYVRRVFIMDNCE++IP+YL
Sbjct: 321 DLFDTKKKPNNIKLYVRRVFIMDNCEDIIPDYL 353
>At4g24190.2 68417.m03473 shepherd protein (SHD) / clavata formation
protein, putative nearly identical to SHEPHERD
[Arabidopsis thaliana] GI:19570872; contains Pfam
profiles PF02518: ATPase, histidine kinase-, DNA gyrase
B-, and HSP90-like domain protein, PF00183: Hsp90
protein
Length = 823
Score = 103 bits (248), Expect = 4e-23
Identities = 49/98 (50%), Positives = 69/98 (70%), Gaps = 4/98 (4%)
Frame = +2
Query: 137 LNKTKPIWTRNADDITQEEYGDFYKSLTNDWEDH--LAVKHFSVEGQLEFRALLFVPRRA 310
LN K IW R+ ++T+EEY FY SL+ D+ D +A HF+ EG +EF+A+L+VP +A
Sbjct: 341 LNDVKAIWLRSPKEVTEEEYTKFYHSLSKDFTDEKPMAWSHFNAEGDVEFKAVLYVPPKA 400
Query: 311 PFDLFENKRRKNK--IKLYVRRVFIMDNCEELIPEYLN 418
P DL+E+ NK +KLYVRRVFI D +EL+P+YL+
Sbjct: 401 PHDLYESYYNSNKANLKLYVRRVFISDEFDELLPKYLS 438
>At4g24190.1 68417.m03472 shepherd protein (SHD) / clavata formation
protein, putative nearly identical to SHEPHERD
[Arabidopsis thaliana] GI:19570872; contains Pfam
profiles PF02518: ATPase, histidine kinase-, DNA gyrase
B-, and HSP90-like domain protein, PF00183: Hsp90
protein
Length = 823
Score = 103 bits (248), Expect = 4e-23
Identities = 49/98 (50%), Positives = 69/98 (70%), Gaps = 4/98 (4%)
Frame = +2
Query: 137 LNKTKPIWTRNADDITQEEYGDFYKSLTNDWEDH--LAVKHFSVEGQLEFRALLFVPRRA 310
LN K IW R+ ++T+EEY FY SL+ D+ D +A HF+ EG +EF+A+L+VP +A
Sbjct: 341 LNDVKAIWLRSPKEVTEEEYTKFYHSLSKDFTDEKPMAWSHFNAEGDVEFKAVLYVPPKA 400
Query: 311 PFDLFENKRRKNK--IKLYVRRVFIMDNCEELIPEYLN 418
P DL+E+ NK +KLYVRRVFI D +EL+P+YL+
Sbjct: 401 PHDLYESYYNSNKANLKLYVRRVFISDEFDELLPKYLS 438
>At3g07770.1 68416.m00947 heat shock protein-related strong
similarity to heat-shock protein [Secale cereale]
GI:556673; contains Pfam profiles PF02518: ATPase,
histidine kinase-, DNA gyrase B-, and HSP90-like domain
protein, PF00183: Hsp90 protein
Length = 803
Score = 93.1 bits (221), Expect = 7e-20
Identities = 45/96 (46%), Positives = 67/96 (69%), Gaps = 3/96 (3%)
Frame = +2
Query: 140 NKTKPIWTRNADDITQEEYGDFYKSLTNDWEDHLAVKHFSVEGQLEFRALLFVPRRAPF- 316
N+T+PIW RN ++T EY +FY+ N++ D LA HF+ EG++EFR++L+VP +P
Sbjct: 353 NETQPIWLRNPKEVTTAEYNEFYRKAFNEYLDPLASSHFTTEGEVEFRSILYVPPVSPSG 412
Query: 317 -DLFENKRRKNKIKLYVRRVFIMDNCE-ELIPEYLN 418
D N++ KN I+LYV+RVFI D+ + EL P YL+
Sbjct: 413 KDDIVNQKTKN-IRLYVKRVFISDDFDGELFPRYLS 447
>At2g04030.2 68415.m00372 heat shock protein, putative strong
similarity to heat shock protein [Arabidopsis thaliana]
GI:1906830; contains Pfam profiles PF02518: ATPase,
histidine kinase-, DNA gyrase B-, and HSP90-like domain
protein, PF00183: Hsp90 protein
Length = 777
Score = 91.5 bits (217), Expect = 2e-19
Identities = 43/96 (44%), Positives = 67/96 (69%), Gaps = 3/96 (3%)
Frame = +2
Query: 140 NKTKPIWTRNADDITQEEYGDFYKSLTNDWEDHLAVKHFSVEGQLEFRALLFVPRRAPFD 319
N+TKP+W RN+ ++ + EY +FYK N++ D LA HF+ EG++EFR++L++P P +
Sbjct: 330 NETKPLWMRNSKEVEKGEYNEFYKKAFNEFLDPLAHTHFTTEGEVEFRSILYIPGMGPLN 389
Query: 320 LFE--NKRRKNKIKLYVRRVFIMDNCE-ELIPEYLN 418
+ N + KN I+LYV+RVFI D+ + EL P YL+
Sbjct: 390 NEDVTNPKTKN-IRLYVKRVFISDDFDGELFPRYLS 424
>At2g04030.1 68415.m00371 heat shock protein, putative strong
similarity to heat shock protein [Arabidopsis thaliana]
GI:1906830; contains Pfam profiles PF02518: ATPase,
histidine kinase-, DNA gyrase B-, and HSP90-like domain
protein, PF00183: Hsp90 protein
Length = 780
Score = 91.5 bits (217), Expect = 2e-19
Identities = 43/96 (44%), Positives = 67/96 (69%), Gaps = 3/96 (3%)
Frame = +2
Query: 140 NKTKPIWTRNADDITQEEYGDFYKSLTNDWEDHLAVKHFSVEGQLEFRALLFVPRRAPFD 319
N+TKP+W RN+ ++ + EY +FYK N++ D LA HF+ EG++EFR++L++P P +
Sbjct: 330 NETKPLWMRNSKEVEKGEYNEFYKKAFNEFLDPLAHTHFTTEGEVEFRSILYIPGMGPLN 389
Query: 320 LFE--NKRRKNKIKLYVRRVFIMDNCE-ELIPEYLN 418
+ N + KN I+LYV+RVFI D+ + EL P YL+
Sbjct: 390 NEDVTNPKTKN-IRLYVKRVFISDDFDGELFPRYLS 424
>At3g17620.1 68416.m02251 F-box family protein contains Pfam
profile: PF00646 F-box domain
Length = 398
Score = 28.3 bits (60), Expect = 2.2
Identities = 13/29 (44%), Positives = 17/29 (58%)
Frame = -3
Query: 415 QVFWDKLFTVVHDKYPSDIKFNLVLASFI 329
+V W KLF V + +D KF + ASFI
Sbjct: 294 EVMWSKLFLAVDMQPLTDFKFGIADASFI 322
>At3g13530.1 68416.m01701 MAP3K epsilon protein kinase identical to
MAP3K epsilon protein kinase [Arabidopsis thaliana]
gi|3549652|emb|CAA12272
Length = 1368
Score = 27.9 bits (59), Expect = 3.0
Identities = 18/61 (29%), Positives = 29/61 (47%)
Frame = +2
Query: 173 DDITQEEYGDFYKSLTNDWEDHLAVKHFSVEGQLEFRALLFVPRRAPFDLFENKRRKNKI 352
D+I + YG YK L + D +A+K S+E ++ + DL +N KN +
Sbjct: 24 DEIGKGAYGRVYKGLDLENGDFVAIKQVSLENIVQEDLNTIMQE---IDLLKNLNHKNIV 80
Query: 353 K 355
K
Sbjct: 81 K 81
>At2g01520.1 68415.m00076 major latex protein-related / MLP-related
low similarity to major latex protein {Papaver
somniferum}[GI:169000] contains Pfam profile PF00407:
Pathogenesis-related protein Bet v I family
Length = 151
Score = 27.9 bits (59), Expect = 3.0
Identities = 13/34 (38%), Positives = 18/34 (52%), Gaps = 1/34 (2%)
Frame = +2
Query: 143 KTKPIWTRNADDITQ-EEYGDFYKSLTNDWEDHL 241
K IW + DD+ + Y F KSL D +DH+
Sbjct: 115 KITMIWEKQNDDMPEPSNYMKFVKSLAADMDDHV 148
>At5g60840.1 68418.m07632 expressed protein predicted protein,
Drosophila melanogaster
Length = 207
Score = 27.5 bits (58), Expect = 3.9
Identities = 12/33 (36%), Positives = 18/33 (54%)
Frame = +2
Query: 179 ITQEEYGDFYKSLTNDWEDHLAVKHFSVEGQLE 277
+ EEY + Y+S D+ +L KHFS + E
Sbjct: 71 LDSEEYAEKYRSYEADFRKYLMDKHFSEVDEYE 103
>At3g07980.1 68416.m00975 protein kinase, putative similar to MAP3K
epsilon protein kinase [Arabidopsis thaliana]
gi|3549652|emb|CAA12272
Length = 1367
Score = 26.6 bits (56), Expect = 6.8
Identities = 19/63 (30%), Positives = 30/63 (47%), Gaps = 2/63 (3%)
Frame = +2
Query: 173 DDITQEEYGDFYKSLTNDWEDHLAVKHFSVE--GQLEFRALLFVPRRAPFDLFENKRRKN 346
D+I + YG Y L + D +A+K S+E GQ + ++ DL +N KN
Sbjct: 24 DEIGKGAYGRVYIGLDLENGDFVAIKQVSLENIGQEDLNTIM-----QEIDLLKNLNHKN 78
Query: 347 KIK 355
+K
Sbjct: 79 IVK 81
>At5g03650.1 68418.m00324 1,4-alpha-glucan branching enzyme / starch
branching enzyme class II (SBE2-2) identical to starch
branching enzyme class II [Arabidopsis thaliana]
GI:726490
Length = 716
Score = 26.2 bits (55), Expect = 9.0
Identities = 12/34 (35%), Positives = 19/34 (55%)
Frame = +2
Query: 158 WTRNADDITQEEYGDFYKSLTNDWEDHLAVKHFS 259
W NAD +T+ E+G + L N+ + A+ H S
Sbjct: 111 WNSNADIMTRNEFGVWEIFLPNNTDGSPAIPHGS 144
>At1g75470.1 68414.m08766 purine permease-related contains Pfam
profile PF03151: Domain of unknown function, DUF250; low
similarity to purine permease [Arabidopsis thaliana]
GI:7620007
Length = 381
Score = 26.2 bits (55), Expect = 9.0
Identities = 10/16 (62%), Positives = 13/16 (81%)
Frame = -3
Query: 397 LFTVVHDKYPSDIKFN 350
LFT++ KY +DIKFN
Sbjct: 154 LFTLIFSKYYNDIKFN 169
>At1g14930.1 68414.m01784 major latex protein-related / MLP-related
low similarity to major latex protein {Papaver
somniferum}[GI:20810] ; contains Pfam profile PF00407:
Pathogenesis-related protein Bet v I family
Length = 155
Score = 26.2 bits (55), Expect = 9.0
Identities = 14/34 (41%), Positives = 16/34 (47%), Gaps = 1/34 (2%)
Frame = +2
Query: 143 KTKPIW-TRNADDITQEEYGDFYKSLTNDWEDHL 241
K IW RN D Y F KSL D +DH+
Sbjct: 115 KVTLIWENRNEDSPEPINYMKFVKSLVADMDDHI 148
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 6,680,802
Number of Sequences: 28952
Number of extensions: 118997
Number of successful extensions: 367
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 356
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 360
length of database: 12,070,560
effective HSP length: 74
effective length of database: 9,928,112
effective search space used: 645327280
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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