Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= S06A01NCLL0001_A24
(409 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha Ef... 212 2e-56
SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha Ef... 212 2e-56
SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha Ef... 212 2e-56
SPCC584.04 |sup35|erf3|translation release factor eRF3 |Schizosa... 74 7e-15
SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related prote... 38 8e-04
SPAC631.01c |acp2||F-actin capping protein beta subunit |Schizos... 27 0.85
SPBC1709.17 |||folylpolyglutamate synthase|Schizosaccharomyces p... 26 2.0
SPAC15E1.05c |||ethanolamine-phosphate cytidylyltransferase |Sch... 26 2.0
SPCC777.03c |||nifs homolog|Schizosaccharomyces pombe|chr 3|||Ma... 26 2.0
SPBPJ4664.05 |||conserved fungal protein|Schizosaccharomyces pom... 25 3.4
SPAC19G12.16c |adg2|SPAC23A1.01c, mug46|conserved fungal protein... 25 3.4
SPAC19D5.04 |ptr1||HECT domain|Schizosaccharomyces pombe|chr 1||... 25 6.0
SPAC227.12 |||U4/U6 x U5 tri-snRNP complex subunit Prp4 family|S... 25 6.0
SPAC31A2.11c |cuf1||Cu metalloregulatory transcription factor Cu... 24 7.9
SPBC21C3.19 |||DUF1960 family protein|Schizosaccharomyces pombe|... 24 7.9
SPBC18H10.04c |sce3|tif48|translation initiation factor eIF4B|Sc... 24 7.9
>SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha
Ef1a-b |Schizosaccharomyces pombe|chr 1|||Manual
Length = 460
Score = 212 bits (517), Expect = 2e-56
Identities = 97/133 (72%), Positives = 111/133 (83%)
Frame = +1
Query: 10 VAGDSKNNPPRGAADFTAQVIVLNHPGQISNGYTPVLDCHTAHIACKFAEIKEKVDRRTG 189
V GDSKN+PP G A FTAQVI+LNHPGQIS GY+PVLDCHTAHIACKFAE+ EK+DRR+G
Sbjct: 323 VCGDSKNDPPMGCASFTAQVIILNHPGQISAGYSPVLDCHTAHIACKFAELIEKIDRRSG 382
Query: 190 KSTEDNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGRFAVRDMRQTVAVGVIKAVNFK 369
K E++PK +KSGDA I +VPSKP+CVE+F ++ PLGRFAVRDMRQTVAVGVIKAV
Sbjct: 383 KKIEESPKFVKSGDACIAKMVPSKPMCVEAFTDYAPLGRFAVRDMRQTVAVGVIKAVEKV 442
Query: 370 EAGGGKVTKAAEK 408
G KVTKAA K
Sbjct: 443 APGAAKVTKAAVK 455
>SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha
Ef1a-a |Schizosaccharomyces pombe|chr 3|||Manual
Length = 460
Score = 212 bits (517), Expect = 2e-56
Identities = 97/133 (72%), Positives = 111/133 (83%)
Frame = +1
Query: 10 VAGDSKNNPPRGAADFTAQVIVLNHPGQISNGYTPVLDCHTAHIACKFAEIKEKVDRRTG 189
V GDSKN+PP G A FTAQVI+LNHPGQIS GY+PVLDCHTAHIACKFAE+ EK+DRR+G
Sbjct: 323 VCGDSKNDPPMGCASFTAQVIILNHPGQISAGYSPVLDCHTAHIACKFAELIEKIDRRSG 382
Query: 190 KSTEDNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGRFAVRDMRQTVAVGVIKAVNFK 369
K E++PK +KSGDA I +VPSKP+CVE+F ++ PLGRFAVRDMRQTVAVGVIKAV
Sbjct: 383 KKIEESPKFVKSGDACIAKMVPSKPMCVEAFTDYAPLGRFAVRDMRQTVAVGVIKAVEKV 442
Query: 370 EAGGGKVTKAAEK 408
G KVTKAA K
Sbjct: 443 APGAAKVTKAAVK 455
>SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha
Ef1a-c |Schizosaccharomyces pombe|chr 2|||Manual
Length = 460
Score = 212 bits (517), Expect = 2e-56
Identities = 97/133 (72%), Positives = 111/133 (83%)
Frame = +1
Query: 10 VAGDSKNNPPRGAADFTAQVIVLNHPGQISNGYTPVLDCHTAHIACKFAEIKEKVDRRTG 189
V GDSKN+PP G A FTAQVI+LNHPGQIS GY+PVLDCHTAHIACKFAE+ EK+DRR+G
Sbjct: 323 VCGDSKNDPPMGCASFTAQVIILNHPGQISAGYSPVLDCHTAHIACKFAELIEKIDRRSG 382
Query: 190 KSTEDNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGRFAVRDMRQTVAVGVIKAVNFK 369
K E++PK +KSGDA I +VPSKP+CVE+F ++ PLGRFAVRDMRQTVAVGVIKAV
Sbjct: 383 KKIEESPKFVKSGDACIAKMVPSKPMCVEAFTDYAPLGRFAVRDMRQTVAVGVIKAVEKV 442
Query: 370 EAGGGKVTKAAEK 408
G KVTKAA K
Sbjct: 443 APGAAKVTKAAVK 455
>SPCC584.04 |sup35|erf3|translation release factor eRF3
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 662
Score = 74.1 bits (174), Expect = 7e-15
Identities = 39/115 (33%), Positives = 62/115 (53%), Gaps = 1/115 (0%)
Frame = +1
Query: 22 SKNNPPRGAADFTAQVIVLNHPGQISNGYTPVLDCHTAHIACKFAEIKEKVDRRTGKSTE 201
S NP F AQ+ +L P ++ GY+ V+ HTA FA++ K+D+ T + ++
Sbjct: 549 STKNPVHATTRFIAQIAILELPSILTTGYSCVMHIHTAVEEVSFAKLLHKLDK-TNRKSK 607
Query: 202 DNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGRFAVRDMRQTVAVG-VIKAVN 363
P G I L P+C+E F+++ +GRF +RD TVAVG V+K ++
Sbjct: 608 KPPMFATKGMKIIAELETQTPVCMERFEDYQYMGRFTLRDQGTTVAVGKVVKILD 662
>SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related
protein|Schizosaccharomyces pombe|chr 2|||Manual
Length = 592
Score = 37.5 bits (83), Expect = 8e-04
Identities = 33/108 (30%), Positives = 47/108 (43%)
Frame = +1
Query: 31 NPPRGAADFTAQVIVLNHPGQISNGYTPVLDCHTAHIACKFAEIKEKVDRRTGKSTEDNP 210
NP R F A++ + G I +G T VL H+ + K+ K + +
Sbjct: 490 NPVRRVRSFVAEIQTFDIHGPILSGSTLVL-----HLGRTVTSVSLKIVTVNNKRSR-HI 543
Query: 211 KSIKSGDAAIVNLVPSKPLCVESFQEFPPLGRFAVRDMRQTVAVGVIK 354
S K I L PLC+ +E P LGRF +R TVA G++K
Sbjct: 544 ASRKRALVRISFLDGLFPLCLA--EECPALGRFILRRSGDTVAAGIVK 589
>SPAC631.01c |acp2||F-actin capping protein beta subunit
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 268
Score = 27.5 bits (58), Expect = 0.85
Identities = 12/25 (48%), Positives = 19/25 (76%)
Frame = -3
Query: 239 MAASPDLMDLGLSSVDLPVRRSTFS 165
++ +PDL D+ LSSVD P++ +T S
Sbjct: 27 LSVAPDLADVLLSSVDQPLKVNTCS 51
>SPBC1709.17 |||folylpolyglutamate synthase|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 505
Score = 26.2 bits (55), Expect = 2.0
Identities = 15/48 (31%), Positives = 24/48 (50%), Gaps = 1/48 (2%)
Frame = +3
Query: 39 QGSCRLHSASHCAKSPRSNIKRIHTCIGLPHSPHSLQIC-RNQRESRP 179
+GS ++S + +S + I CIG+ SPH +C R Q +P
Sbjct: 90 KGSTCAFTSSILQQIQKSGERSIPKCIGMYTSPHLRSVCERIQLNGKP 137
>SPAC15E1.05c |||ethanolamine-phosphate cytidylyltransferase
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 365
Score = 26.2 bits (55), Expect = 2.0
Identities = 15/54 (27%), Positives = 28/54 (51%)
Frame = -3
Query: 260 LEGTRLTMAASPDLMDLGLSSVDLPVRRSTFSLISANLQAMWAVWQSNTGVYPF 99
LE R ++ +L+D LSSV L + + S++S+ + + + G+ PF
Sbjct: 122 LEVKRTEGVSTTELLDRLLSSVPLEIYSTPVSVLSSQIDLLRRFATDSDGLTPF 175
>SPCC777.03c |||nifs homolog|Schizosaccharomyces pombe|chr
3|||Manual
Length = 396
Score = 26.2 bits (55), Expect = 2.0
Identities = 14/42 (33%), Positives = 22/42 (52%), Gaps = 2/42 (4%)
Frame = -3
Query: 224 DLMDLGLSSVDL--PVRRSTFSLISANLQAMWAVWQSNTGVY 105
+L LG++ + P +RST S ++ L W + N GVY
Sbjct: 316 ELSKLGVTIIGSKDPKKRSTHSYVAKILNPEWDAFLKNEGVY 357
>SPBPJ4664.05 |||conserved fungal protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 163
Score = 25.4 bits (53), Expect = 3.4
Identities = 11/34 (32%), Positives = 19/34 (55%)
Frame = -2
Query: 183 TTVDFLFDFGKFAGYVGCVAIQYRCVSV*YLTWV 82
T +D+LF F+ +G + Y ++V Y+ WV
Sbjct: 73 TLIDYLFFSPPFSLSIGPSLLVYLSIAVSYMLWV 106
>SPAC19G12.16c |adg2|SPAC23A1.01c, mug46|conserved fungal
protein|Schizosaccharomyces pombe|chr 1|||Manual
Length = 670
Score = 25.4 bits (53), Expect = 3.4
Identities = 24/85 (28%), Positives = 43/85 (50%), Gaps = 2/85 (2%)
Frame = -3
Query: 404 SAALVTLPPPASLKFTAFITPTATVCLMSRTAKRPRGGNSWKDSTH--RGLEGTRLTMAA 231
S +VTLPPPAS ++F T T T + S ++ G+ + +++ + ++++
Sbjct: 183 STDIVTLPPPAS-STSSFSTITNTSMIPSSSSFTTTTGSPYYNTSSFLPSSVISSASLSS 241
Query: 230 SPDLMDLGLSSVDLPVRRSTFSLIS 156
S L ++S PV S+ SL S
Sbjct: 242 SSVLPTSIITSTSTPVTVSSSSLSS 266
>SPAC19D5.04 |ptr1||HECT domain|Schizosaccharomyces pombe|chr
1|||Manual
Length = 3227
Score = 24.6 bits (51), Expect = 6.0
Identities = 9/30 (30%), Positives = 18/30 (60%)
Frame = -2
Query: 294 WEFLEGLHTQGLGGNQVDNGGITRFNGFRV 205
++FL+ + + LGGN + G+T G+ +
Sbjct: 389 YDFLQFMTSSPLGGNMACSAGLTSLLGYHL 418
>SPAC227.12 |||U4/U6 x U5 tri-snRNP complex subunit Prp4
family|Schizosaccharomyces pombe|chr 1|||Manual
Length = 462
Score = 24.6 bits (51), Expect = 6.0
Identities = 8/19 (42%), Positives = 12/19 (63%)
Frame = -3
Query: 164 LISANLQAMWAVWQSNTGV 108
L+SA+ W +W +TGV
Sbjct: 280 LVSASFDTTWRLWDVHTGV 298
>SPAC31A2.11c |cuf1||Cu metalloregulatory transcription factor Cuf1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 411
Score = 24.2 bits (50), Expect = 7.9
Identities = 11/25 (44%), Positives = 14/25 (56%)
Frame = +1
Query: 76 LNHPGQISNGYTPVLDCHTAHIACK 150
L HP Q+SN +T C A AC+
Sbjct: 305 LPHPIQLSNYFTLPSSCAQADAACQ 329
>SPBC21C3.19 |||DUF1960 family protein|Schizosaccharomyces pombe|chr
2|||Manual
Length = 106
Score = 24.2 bits (50), Expect = 7.9
Identities = 11/30 (36%), Positives = 17/30 (56%)
Frame = -2
Query: 387 FATTCFLEVHSLYYTDGDRLPHVTHGETTE 298
F T+ ++V + TDG +PH HG+ E
Sbjct: 75 FGTSKDVDVVTKILTDGKIIPHREHGKHKE 104
>SPBC18H10.04c |sce3|tif48|translation initiation factor
eIF4B|Schizosaccharomyces pombe|chr 2|||Manual
Length = 388
Score = 24.2 bits (50), Expect = 7.9
Identities = 13/41 (31%), Positives = 19/41 (46%)
Frame = -3
Query: 392 VTLPPPASLKFTAFITPTATVCLMSRTAKRPRGGNSWKDST 270
+ L P +S TP+AT S+ + P GG D+T
Sbjct: 244 LNLKPRSSSNVNTEATPSATTTTSSKPKRDPFGGAKPVDNT 284
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.315 0.132 0.386
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,938,327
Number of Sequences: 5004
Number of extensions: 42417
Number of successful extensions: 115
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 112
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 114
length of database: 2,362,478
effective HSP length: 66
effective length of database: 2,032,214
effective search space used: 140222766
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.6 bits)
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