Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= P5PG1041
(364 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g59630.1 68416.m06653 diphthamide synthesis DPH2 family prote... 77 5e-15
At5g62030.1 68418.m07786 diphthamide synthesis DPH2 family prote... 46 1e-05
At5g35753.1 68418.m04282 expressed protein 29 0.70
At4g25880.1 68417.m03721 pumilio/Puf RNA-binding domain-containi... 29 0.70
At2g36380.1 68415.m04464 ABC transporter family protein related ... 29 0.93
At3g30560.1 68416.m03867 hypothetical protein 28 1.6
At2g31130.1 68415.m03801 expressed protein 28 2.1
At2g02640.1 68415.m00203 DC1 domain-containing protein contain... 27 2.8
At5g08650.1 68418.m01029 GTP-binding protein LepA, putative 27 3.7
At4g25880.2 68417.m03722 pumilio/Puf RNA-binding domain-containi... 27 3.7
At3g42320.1 68416.m04373 hypothetical protein various predicted ... 27 3.7
At2g14500.1 68415.m01623 F-box family protein contains F-box dom... 27 4.9
At2g02620.1 68415.m00201 DC1 domain-containing protein / PHD fin... 27 4.9
At1g07360.1 68414.m00785 zinc finger (CCCH-type) family protein ... 27 4.9
At4g14000.1 68417.m02165 expressed protein 26 6.5
At2g29580.1 68415.m03592 zinc finger (CCCH-type) family protein ... 26 6.5
At2g05250.1 68415.m00553 DNAJ heat shock N-terminal domain-conta... 26 8.6
At2g05230.1 68415.m00551 DNAJ heat shock N-terminal domain-conta... 26 8.6
At2g02610.1 68415.m00200 DC1 domain-containing protein contain... 26 8.6
>At3g59630.1 68416.m06653 diphthamide synthesis DPH2 family protein
contains InterPro accession IPR002728: Diphthamide
synthesis DPH2 protein
Length = 491
Score = 76.6 bits (180), Expect = 5e-15
Identities = 34/86 (39%), Positives = 52/86 (60%), Gaps = 6/86 (6%)
Frame = +2
Query: 125 TNFDIKETCKWIERKKFAKVCLQFPDDLLEVSCKVYKEIKDRI------EVDLYIMGDTS 286
+ ++I T ++I K F ++ LQFPD+LL+ S KV +K + EV ++M DT+
Sbjct: 7 SKYEINRTAEFIISKSFTRIALQFPDELLKDSTKVVSALKSKTRLLTDREVRFFVMADTT 66
Query: 287 YASCSIDSVAAMHVQANGIVHYGHKC 364
Y SC ID V A+H+ + +VHYG C
Sbjct: 67 YGSCCIDEVGALHIDSECVVHYGQTC 92
>At5g62030.1 68418.m07786 diphthamide synthesis DPH2 family protein
contains InterPro accession IPR002728: Diphthamide
synthesis DPH2 protein
Length = 453
Score = 45.6 bits (103), Expect = 1e-05
Identities = 22/83 (26%), Positives = 43/83 (51%), Gaps = 2/83 (2%)
Frame = +2
Query: 122 ITNFDIKETCKW-IERKKFAKVCLQFPDDLLEVSCKVYKEIKDRIEVD-LYIMGDTSYAS 295
I +F++ + C W I+ ++ LQ P+ LL + + +++GD +Y +
Sbjct: 46 IYHFEVHK-CVWRIKTSNAKRIALQLPEGLLMYALTLSDIFTSFAGASHCFVLGDVTYGA 104
Query: 296 CSIDSVAAMHVQANGIVHYGHKC 364
C +D +A + A+ ++HYGH C
Sbjct: 105 CCVDDFSACALGADLLIHYGHSC 127
>At5g35753.1 68418.m04282 expressed protein
Length = 592
Score = 29.5 bits (63), Expect = 0.70
Identities = 15/45 (33%), Positives = 23/45 (51%), Gaps = 1/45 (2%)
Frame = +2
Query: 98 KTEGYDDIITNFDIKETCKWIERKKFAKVCLQFPDDLL-EVSCKV 229
K +GY + D +E+ KWI R++ + Q P L E +C V
Sbjct: 524 KVDGYKTVYCRRDKEESKKWIPRREMLRFSHQVPSRFLKEETCGV 568
>At4g25880.1 68417.m03721 pumilio/Puf RNA-binding domain-containing
protein contains Pfam profile:PF00806 Pumilio-family RNA
binding domains
Length = 861
Score = 29.5 bits (63), Expect = 0.70
Identities = 21/84 (25%), Positives = 41/84 (48%), Gaps = 5/84 (5%)
Frame = +2
Query: 26 YVKNKMGNFTTEDKICITREITVNKTEGYDDIITNFDIKETCKW---IERKKFAKVCLQ- 193
+++ K+ N E+K + REI + + D+ N+ I++ ++ +RK+ A +
Sbjct: 549 FIQQKLENCKPEEKAAVFREILPHACKLMTDVFGNYVIQKFFEYGNSTQRKELADQLMGQ 608
Query: 194 -FPDDLLEVSCKVYKEIKDRIEVD 262
P L C+V ++ D IE D
Sbjct: 609 IVPLSLQMYGCRVIQKALDVIEPD 632
>At2g36380.1 68415.m04464 ABC transporter family protein related to
multi drug resistance proteins and P-glycoproteins
Length = 1453
Score = 29.1 bits (62), Expect = 0.93
Identities = 14/32 (43%), Positives = 19/32 (59%), Gaps = 2/32 (6%)
Frame = -1
Query: 154 FACFLYIKIG-DYIVITFSFIYCY-LTCYTYF 65
+ LY IG D+ V+ F + Y Y LTC+ YF
Sbjct: 1294 YTLILYSMIGYDWTVVKFFWFYYYMLTCFVYF 1325
>At3g30560.1 68416.m03867 hypothetical protein
Length = 1473
Score = 28.3 bits (60), Expect = 1.6
Identities = 18/70 (25%), Positives = 33/70 (47%), Gaps = 3/70 (4%)
Frame = +2
Query: 107 GYDDIITNFDIKETCKWIERKKFAKVCLQFPDDLLEVSCKVYKEIKDRIEVDL---YIMG 277
G+ D+ F KW + +F K +D ++ C++YK + + DL +I G
Sbjct: 490 GFPDLFITFTCNS--KWPKITRFVKERKLNAEDKPDIICRIYKMKLENLMDDLTKNHIFG 547
Query: 278 DTSYASCSID 307
T A+ +I+
Sbjct: 548 KTKLATYTIE 557
>At2g31130.1 68415.m03801 expressed protein
Length = 419
Score = 27.9 bits (59), Expect = 2.1
Identities = 14/39 (35%), Positives = 23/39 (58%)
Frame = +2
Query: 137 IKETCKWIERKKFAKVCLQFPDDLLEVSCKVYKEIKDRI 253
++E+C ++R +F V FPD + K YK+I+D I
Sbjct: 333 VEESCILVDRDEFHSV---FPDKMENDKHKPYKKIRDAI 368
>At2g02640.1 68415.m00203 DC1 domain-containing protein contains
Pfam profile PF03107: DC1 domain
Length = 627
Score = 27.5 bits (58), Expect = 2.8
Identities = 15/56 (26%), Positives = 27/56 (48%)
Frame = +2
Query: 5 FYHKLKNYVKNKMGNFTTEDKICITREITVNKTEGYDDIITNFDIKETCKWIERKK 172
FYH +++K + +K C + +N+ Y + +F + ETC + RKK
Sbjct: 348 FYHH--HHLKFDISGVYVGNKFCQACVLPINEGNIYVCVECDFILHETCAYAPRKK 401
>At5g08650.1 68418.m01029 GTP-binding protein LepA, putative
Length = 681
Score = 27.1 bits (57), Expect = 3.7
Identities = 14/39 (35%), Positives = 21/39 (53%)
Frame = +2
Query: 245 DRIEVDLYIMGDTSYASCSIDSVAAMHVQANGIVHYGHK 361
++I+VD G+ Y + S+ SVA V + I HY K
Sbjct: 324 NQIQVDELYAGEVGYIAASVRSVADARV-GDTITHYSRK 361
>At4g25880.2 68417.m03722 pumilio/Puf RNA-binding domain-containing
protein contains Pfam profile:PF00806 Pumilio-family RNA
binding domains
Length = 852
Score = 27.1 bits (57), Expect = 3.7
Identities = 21/82 (25%), Positives = 39/82 (47%), Gaps = 5/82 (6%)
Frame = +2
Query: 32 KNKMGNFTTEDKICITREITVNKTEGYDDIITNFDIKETCKW---IERKKFAKVCLQ--F 196
+ K+ N E+K + REI + + D+ N+ I++ ++ +RK+ A +
Sbjct: 542 QQKLENCKPEEKAAVFREILPHACKLMTDVFGNYVIQKFFEYGNSTQRKELADQLMGQIV 601
Query: 197 PDDLLEVSCKVYKEIKDRIEVD 262
P L C+V ++ D IE D
Sbjct: 602 PLSLQMYGCRVIQKALDVIEPD 623
>At3g42320.1 68416.m04373 hypothetical protein various predicted
Helicases, Arabidopsis thaliana
Length = 541
Score = 27.1 bits (57), Expect = 3.7
Identities = 21/73 (28%), Positives = 35/73 (47%), Gaps = 5/73 (6%)
Frame = +2
Query: 146 TC--KWIERKKFAKVCLQFPDDLLEVSCKVYKEIKDRIEVDL---YIMGDTSYASCSIDS 310
TC KW E +F K +D ++ C+++K D + DL Y++G T SC
Sbjct: 166 TCNPKWHEIVRFYKARNLKSEDRPDIICQIFKMKLDNLMYDLTTKYLLGKT--VSC---- 219
Query: 311 VAAMHVQANGIVH 349
+ + Q G++H
Sbjct: 220 MYTIEFQKRGLLH 232
>At2g14500.1 68415.m01623 F-box family protein contains F-box domain
Pfam:PF00646
Length = 347
Score = 26.6 bits (56), Expect = 4.9
Identities = 10/13 (76%), Positives = 11/13 (84%)
Frame = -1
Query: 148 CFLYIKIGDYIVI 110
CFLYI +GDYI I
Sbjct: 215 CFLYIGVGDYIQI 227
>At2g02620.1 68415.m00201 DC1 domain-containing protein / PHD finger
protein-related contains Pfam profiles PF03107: DC1
domain, weak hit to PF00628: PHD-finger
Length = 513
Score = 26.6 bits (56), Expect = 4.9
Identities = 13/39 (33%), Positives = 19/39 (48%)
Frame = +2
Query: 59 EDKICITREITVNKTEGYDDIITNFDIKETCKWIERKKF 175
E+K C + +N+ Y + F + ETC RKKF
Sbjct: 248 EEKFCQACVLPINEKNLYVCVECGFILHETCADAPRKKF 286
>At1g07360.1 68414.m00785 zinc finger (CCCH-type) family protein /
RNA recognition motif (RRM)-containing protein similar
to SP|O59800 Cell cycle control protein cwf5
{Schizosaccharomyces pombe}, RNA Binding Protein 47
[Nicotiana plumbaginifolia] GI:9663769; contains Pfam
profile: PF00076 RNA recognition motif (aka RRM, RBD, or
RNP domain)
Length = 481
Score = 26.6 bits (56), Expect = 4.9
Identities = 20/56 (35%), Positives = 27/56 (48%), Gaps = 1/56 (1%)
Frame = +2
Query: 26 YVKNKMGNFTTEDKICITREITVNK-TEGYDDIITNFDIKETCKWIERKKFAKVCL 190
YV+ N+ E KIC TR TV + G D +I +TC + K +VCL
Sbjct: 32 YVRMTKANYDKECKIC-TRPFTVFRWRPGRDARYKKTEICQTC--CKLKNVCQVCL 84
>At4g14000.1 68417.m02165 expressed protein
Length = 290
Score = 26.2 bits (55), Expect = 6.5
Identities = 14/46 (30%), Positives = 21/46 (45%)
Frame = +2
Query: 95 NKTEGYDDIITNFDIKETCKWIERKKFAKVCLQFPDDLLEVSCKVY 232
NK GYD I+ I + + K CL +PD + ++ K Y
Sbjct: 200 NKKGGYDIILMAETIYSISAQKSQYELIKRCLAYPDGAVYMAAKKY 245
>At2g29580.1 68415.m03592 zinc finger (CCCH-type) family protein /
RNA recognition motif (RRM)-containing protein similar
to SP|O59800 Cell cycle control protein cwf5
{Schizosaccharomyces pombe}; contains Pfam profile:
PF00076 RNA recognition motif (aka RRM, RBD, or RNP
domain)
Length = 483
Score = 26.2 bits (55), Expect = 6.5
Identities = 19/56 (33%), Positives = 27/56 (48%), Gaps = 1/56 (1%)
Frame = +2
Query: 26 YVKNKMGNFTTEDKICITREITVNK-TEGYDDIITNFDIKETCKWIERKKFAKVCL 190
YV+ N+ E KIC TR TV + G D ++ +TC + K +VCL
Sbjct: 32 YVRMTKANYDKECKIC-TRPFTVFRWRPGRDARYKKTEVCQTC--CKLKNVCQVCL 84
>At2g05250.1 68415.m00553 DNAJ heat shock N-terminal
domain-containing protein contains Pfam profile PF00226
DnaJ domain
Length = 706
Score = 25.8 bits (54), Expect = 8.6
Identities = 15/66 (22%), Positives = 30/66 (45%)
Frame = +2
Query: 41 MGNFTTEDKICITREITVNKTEGYDDIITNFDIKETCKWIERKKFAKVCLQFPDDLLEVS 220
+ +T + +C+T + K EGY + +++ KWI R + + Q P L+ +
Sbjct: 622 LDEYTEQYGVCVTPLV---KLEGYKTVYHRSTREDSKKWIPRCEMLRFSHQVPSWFLKDA 678
Query: 221 CKVYKE 238
+ E
Sbjct: 679 TSGFPE 684
>At2g05230.1 68415.m00551 DNAJ heat shock N-terminal
domain-containing protein contains Pfam profile PF00226
DnaJ domain
Length = 706
Score = 25.8 bits (54), Expect = 8.6
Identities = 15/66 (22%), Positives = 30/66 (45%)
Frame = +2
Query: 41 MGNFTTEDKICITREITVNKTEGYDDIITNFDIKETCKWIERKKFAKVCLQFPDDLLEVS 220
+ +T + +C+T + K EGY + +++ KWI R + + Q P L+ +
Sbjct: 622 LDEYTEQYGVCVTPLV---KLEGYKTVYHRSTREDSKKWIPRCEMLRFSHQVPSWFLKDA 678
Query: 221 CKVYKE 238
+ E
Sbjct: 679 TSGFPE 684
>At2g02610.1 68415.m00200 DC1 domain-containing protein contains
Pfam profile PF03107: DC1 domain
Length = 627
Score = 25.8 bits (54), Expect = 8.6
Identities = 12/51 (23%), Positives = 25/51 (49%)
Frame = +2
Query: 20 KNYVKNKMGNFTTEDKICITREITVNKTEGYDDIITNFDIKETCKWIERKK 172
++++K + +K C + +N+ Y + +F + ETC + RKK
Sbjct: 351 RHHLKFDISGVFVGNKFCQACVLPINEGNVYVCVECDFILHETCAYAPRKK 401
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 7,928,691
Number of Sequences: 28952
Number of extensions: 151674
Number of successful extensions: 388
Number of sequences better than 10.0: 19
Number of HSP's better than 10.0 without gapping: 383
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 387
length of database: 12,070,560
effective HSP length: 73
effective length of database: 9,957,064
effective search space used: 467982008
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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